BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP16_FL5_M14
(883 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.16
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 30 0.50
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 1.5
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 28 1.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 2.7
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 2.7
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.5
SPAC56E4.02c |alg13||N-acetylglucosaminyldiphosphodolichol N-ace... 27 4.7
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.5 bits (68), Expect = 0.16
Identities = 24/101 (23%), Positives = 28/101 (27%)
Frame = -2
Query: 561 VXPXXXGPWXXAXXXXPXPXXXGXXAXXXXRXXGGSPPPPGXXXXRXXRXPXXPPXXGXP 382
+ P P + P P + PPPP R P P G
Sbjct: 275 IAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKP--PIGNGS- 331
Query: 381 XPSGGXXPPPPPXVXKKXGXXXXPPXLXKXXPPXXXPRGGP 259
S PPPPP P + PP PR P
Sbjct: 332 --SNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAP 370
Score = 29.5 bits (63), Expect = 0.66
Identities = 20/75 (26%), Positives = 23/75 (30%), Gaps = 2/75 (2%)
Frame = -1
Query: 466 PRGEPXXPGXGPXXAAXXPTXTXXXGXPX-PXWGXPPPPPXAXXKKXXXXXXP-PPSLXK 293
PR P P ++ P P P PP + P PPSL
Sbjct: 366 PRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPP 425
Query: 292 XPPPXXPPXGPXXXP 248
PP PP P P
Sbjct: 426 SAPPSLPPSAPPSLP 440
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 29.9 bits (64), Expect = 0.50
Identities = 16/42 (38%), Positives = 17/42 (40%)
Frame = -1
Query: 385 PXPXWGXPPPPPXAXXKKXXXXXXPPPSLXKXPPPXXPPXGP 260
P P G PPPPP PPP + PP PP P
Sbjct: 754 PAPIMGGPPPPP------------PPPGVAGAGPPPPPPPPP 783
Score = 29.1 bits (62), Expect = 0.88
Identities = 16/49 (32%), Positives = 16/49 (32%)
Frame = -2
Query: 453 PPPPGXXXXRXXRXPXXPPXXGXPXPSGGXXPPPPPXVXKKXGXXXXPP 307
PPPP P P P G PPPPP V PP
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPA-PIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 28.3 bits (60), Expect = 1.5
Identities = 15/38 (39%), Positives = 17/38 (44%), Gaps = 4/38 (10%)
Frame = -1
Query: 364 PPPPPXAXX---KKXXXXXXPPPS-LXKXPPPXXPPXG 263
PPPPP A PPP+ + PPP PP G
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPG 769
Score = 26.2 bits (55), Expect = 6.2
Identities = 14/48 (29%), Positives = 17/48 (35%), Gaps = 1/48 (2%)
Frame = -2
Query: 411 PXXPPXXGXPXPSGGXXP-PPPPXVXKKXGXXXXPPXLXKXXPPXXXP 271
P PP P P+ P PPP + PP + PP P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.3 bits (60), Expect = 1.5
Identities = 12/27 (44%), Positives = 13/27 (48%)
Frame = -2
Query: 411 PXXPPXXGXPXPSGGXXPPPPPXVXKK 331
P PP G PS PPPP V K+
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPPGYVKKR 36
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 28.3 bits (60), Expect = 1.5
Identities = 18/66 (27%), Positives = 20/66 (30%)
Frame = -1
Query: 466 PRGEPXXPGXGPXXAAXXPTXTXXXGXPXPXWGXPPPPPXAXXKKXXXXXXPPPSLXKXP 287
P P P P A P P P PP P A K P ++ P
Sbjct: 140 PTSAPPRPSIPPPSPASAPPIPSK-APPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMP 198
Query: 286 PPXXPP 269
P PP
Sbjct: 199 PKVPPP 204
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.5 bits (58), Expect = 2.7
Identities = 14/41 (34%), Positives = 16/41 (39%), Gaps = 2/41 (4%)
Frame = -1
Query: 364 PPPPPXAXXKKXXXXXXPPPSLXKXPPPXXP--PXGPXXXP 248
P P A + PPP + PPP P P GP P
Sbjct: 1691 PVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAP 1731
Score = 27.1 bits (57), Expect = 3.5
Identities = 14/41 (34%), Positives = 14/41 (34%)
Frame = -1
Query: 385 PXPXWGXPPPPPXAXXKKXXXXXXPPPSLXKXPPPXXPPXG 263
P P PPPP A PPP L P P G
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPG 1747
Score = 26.2 bits (55), Expect = 6.2
Identities = 12/42 (28%), Positives = 14/42 (33%)
Frame = -2
Query: 471 RXXGGSPPPPGXXXXRXXRXPXXPPXXGXPXPSGGXXPPPPP 346
R +PP PP P P+G PPPP
Sbjct: 1693 RPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 25.8 bits (54), Expect = 8.2
Identities = 12/39 (30%), Positives = 13/39 (33%)
Frame = -1
Query: 364 PPPPPXAXXKKXXXXXXPPPSLXKXPPPXXPPXGPXXXP 248
PP P + PPP PPP P P P
Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPP 1728
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 27.5 bits (58), Expect = 2.7
Identities = 14/34 (41%), Positives = 15/34 (44%)
Frame = -1
Query: 364 PPPPPXAXXKKXXXXXXPPPSLXKXPPPXXPPXG 263
PPPPP A K P +L PP PP G
Sbjct: 1883 PPPPPMALPKAGPPSAAPTSAL----PPAGPPAG 1912
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.1 bits (57), Expect = 3.5
Identities = 17/48 (35%), Positives = 17/48 (35%), Gaps = 2/48 (4%)
Frame = +3
Query: 249 GXXXGPXGGXXGGGXFXKXGGGXXXNXXFXXXAXGGGGGXP--HXGXG 386
G GP G G G F GG GG GG P H G G
Sbjct: 225 GFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGPG 272
Score = 25.8 bits (54), Expect = 8.2
Identities = 17/49 (34%), Positives = 17/49 (34%), Gaps = 1/49 (2%)
Frame = +3
Query: 249 GXXXGPXGGXXGG-GXFXKXGGGXXXNXXFXXXAXGGGGGXPHXGXGXP 392
G G GG GG G F GG GG GG P G P
Sbjct: 217 GHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGP 265
>SPAC56E4.02c |alg13||N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase Alg13
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 26.6 bits (56), Expect = 4.7
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = -2
Query: 165 YVVRSESIMRDSDVAFSHSAALAIAQVRRNGNK 67
Y ES + D+ + SH+ A +I Q R+G +
Sbjct: 63 YAPEIESYIHDASIVISHAGAGSILQTLRSGKR 95
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,941,118
Number of Sequences: 5004
Number of extensions: 29520
Number of successful extensions: 163
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 60
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -