BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP16_FL5_I21
(810 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 29 0.78
SPAC1F7.04 |rho1||Rho family GTPase Rho1|Schizosaccharomyces pom... 29 1.0
SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 28 1.8
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 27 4.2
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 7.3
SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 25 9.6
SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat protei... 25 9.6
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 29.1 bits (62), Expect = 0.78
Identities = 22/88 (25%), Positives = 43/88 (48%), Gaps = 1/88 (1%)
Frame = +1
Query: 178 FNKIMSTLKLXSEVRSGLYSHWRDVFEHVRSTAKIKHYADQNTSSGTPQEREC-SIRKSC 354
F+K++ST+ +E+ + L WRD +HV + + +S C
Sbjct: 384 FSKMLSTIS--NEI-TNLQGDWRDQLDHVEFLQEHLGPLEVELASVKVLYDNCFQAGIEE 440
Query: 355 NNVSVTSMEDLSHEEIVTSYVLAHVAQF 438
N+ ++ S EDL HE +T+ ++A+ ++
Sbjct: 441 NDYTMFSYEDLEHEFGITANIIANKIKY 468
>SPAC1F7.04 |rho1||Rho family GTPase Rho1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 202
Score = 28.7 bits (61), Expect = 1.0
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +1
Query: 244 RDVFEHVRSTAKIKHYADQNTSSGTPQEREC 336
R+VFE A +KH SSGT +++ C
Sbjct: 169 REVFESATRAAMLKHKPKVKPSSGTKKKKRC 199
>SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2812
Score = 27.9 bits (59), Expect = 1.8
Identities = 15/50 (30%), Positives = 22/50 (44%)
Frame = +1
Query: 253 FEHVRSTAKIKHYADQNTSSGTPQERECSIRKSCNNVSVTSMEDLSHEEI 402
F+ + K K Y Q SG + SIRK+C N+ + L H +
Sbjct: 1709 FDFNGNNKKQKEYVTQLILSGLLNKNTNSIRKTCMNILLYLRRQLGHHAL 1758
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 26.6 bits (56), Expect = 4.2
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +1
Query: 355 NNVSVTSMEDLSHEEIVTSYVLAHVAQFDCRRHHMAFTNGNT 480
+N+SVT+ L + +V++Y L CR AFTNG T
Sbjct: 853 SNISVTNENRLQYIHLVSNYYLNARLSRQCR----AFTNGFT 890
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 7.3
Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 10/78 (12%)
Frame = -2
Query: 659 IHSLMSWFTVLVPYNSS----SSHWLKNDSCRN----YPLVMTDPLLRS--KFHLRFALA 510
++SL+SW + +NS S+ +L+ D C+N Y ++ L + FHL A
Sbjct: 53 VNSLLSWCMLSSWFNSKLSKLSAGYLQFD-CQNDGKCYSVIAVHRLSFTLVMFHLFLAFI 111
Query: 509 LISCNSLLRVVFPLVNAM 456
L CN+ RV + N +
Sbjct: 112 LSLCNTRSRVAIKIQNGL 129
>SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 163
Score = 25.4 bits (53), Expect = 9.6
Identities = 15/62 (24%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
Frame = -2
Query: 653 SLMSWFTVLVPYNSSSSHWLKNDSCRNYP-LVMTDPLLRSKFHLRFALALISCNSLLRVV 477
SL+ + ++ V Y + + YP L + DP+ ++ F+ ++ SC +L++V
Sbjct: 91 SLLVYLSIAVSYMLWVEKCYQMNKFYAYPILAILDPIKKTIFYTVASIISFSCYIVLKMV 150
Query: 476 FP 471
P
Sbjct: 151 HP 152
>SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 945
Score = 25.4 bits (53), Expect = 9.6
Identities = 8/48 (16%), Positives = 22/48 (45%)
Frame = +1
Query: 223 SGLYSHWRDVFEHVRSTAKIKHYADQNTSSGTPQERECSIRKSCNNVS 366
S HW D ++ +T+++ HY++ + + + + ++S
Sbjct: 372 SNFEQHWYDAEDYESTTSQLNHYSESGAHAADATKSSVAHNEKVEDIS 419
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,007,600
Number of Sequences: 5004
Number of extensions: 57455
Number of successful extensions: 132
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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