Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP16_FL5_E20
(837 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
DQ667183-1|ABG75735.1| 463|Apis mellifera GABA-gated ion channe... 33 0.002
DQ026039-1|AAY87898.1| 427|Apis mellifera nicotinic acetylcholi... 33 0.002
DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 28 0.093
DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 28 0.093
DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholi... 28 0.12
DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholi... 28 0.12
DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated... 27 0.21
AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic ac... 27 0.21
AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic ac... 27 0.28
AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 26 0.50
DQ026036-1|AAY87895.1| 529|Apis mellifera nicotinic acetylcholi... 25 0.65
DQ026035-1|AAY87894.1| 529|Apis mellifera nicotinic acetylcholi... 25 0.65
AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic ac... 25 0.65
EF127805-1|ABL67942.1| 461|Apis mellifera nicotinic acetylcholi... 25 0.86
EF127804-1|ABL67941.1| 461|Apis mellifera nicotinic acetylcholi... 25 0.86
EF127803-1|ABL67940.1| 461|Apis mellifera nicotinic acetylcholi... 25 0.86
EF127802-1|ABL67939.1| 461|Apis mellifera nicotinic acetylcholi... 25 0.86
EF127801-1|ABL67938.1| 461|Apis mellifera nicotinic acetylcholi... 25 0.86
EF127800-1|ABL67937.1| 461|Apis mellifera nicotinic acetylcholi... 25 0.86
DQ667182-1|ABG75734.1| 445|Apis mellifera GABA-gated chloride c... 25 0.86
DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholi... 25 0.86
DQ667181-1|ABG75733.1| 445|Apis mellifera GABA-gated chloride c... 24 2.0
>DQ667183-1|ABG75735.1| 463|Apis mellifera GABA-gated ion channel
protein.
Length = 463
Score = 33.5 bits (73), Expect = 0.002
Identities = 19/71 (26%), Positives = 37/71 (52%), Gaps = 4/71 (5%)
Frame = +2
Query: 119 NATSLSLMIDSLLATYDRESPPD---SKIVVNLTLHLRHAN-IRESESTVRILADLQMNW 286
N +++S ++D+LL YD PD V + + +R I E + T + + +W
Sbjct: 2 NHSNISELLDNLLRGYDNSVRPDFGGPPATVEVDIMVRSMGPISEVDMTYSMDCYFRQSW 61
Query: 287 IDKRLSWNAGR 319
+D+RL++ G+
Sbjct: 62 VDRRLAFQGGK 72
>DQ026039-1|AAY87898.1| 427|Apis mellifera nicotinic acetylcholine
receptor beta2subunit protein.
Length = 427
Score = 33.5 bits (73), Expect = 0.002
Identities = 20/72 (27%), Positives = 34/72 (47%), Gaps = 5/72 (6%)
Frame = +2
Query: 113 CENATSLSLMIDS---LLATYDRESPPDSKIV--VNLTLHLRHANIRESESTVRILADLQ 277
C++ TS S + L YDR+ P+ K ++ L ++H N+ E TV L+
Sbjct: 28 CKDITSTSALYRLKLYLFCDYDRDIIPEQKNATKIDFGLSIQHYNVDEYSHTVDFHVMLK 87
Query: 278 MNWIDKRLSWNA 313
+ W L+W +
Sbjct: 88 LMWEQSHLTWKS 99
>DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 28.3 bits (60), Expect = 0.093
Identities = 20/65 (30%), Positives = 31/65 (47%), Gaps = 4/65 (6%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAAATTAGDYAL----RARVSNXXSVSWIKRLDMSTPISXQLDNWP 509
V S+ +WRPD+VL N A G+Y + +A V V W + S ++ +P
Sbjct: 105 VPSDHIWRPDIVLYNNA---DGNYEVTLMTKATVYYSGLVVWQPPAVYKSSCSIDVEFFP 161
Query: 510 NDLXT 524
D+ T
Sbjct: 162 YDVQT 166
>DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 28.3 bits (60), Expect = 0.093
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLL-NAAATTAGDYALRARVSNXXSVSW 455
V S+ +WRPD+VL NA A +A + + V W
Sbjct: 105 VPSDHIWRPDIVLYNNADGNFEVTLATKATIYHQGLVEW 143
>DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholine
receptor alpha3subunit protein.
Length = 566
Score = 27.9 bits (59), Expect = 0.12
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLL-NAAATTAGDYALRARVSNXXSVSW 455
V S+ +WRPD+VL NA A +A ++ V W
Sbjct: 101 VPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEW 139
>DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholine
receptor alpha1subunit protein.
Length = 601
Score = 27.9 bits (59), Expect = 0.12
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = +3
Query: 324 GCSTWLVSSERLWRPDVVLLNAA 392
G T V SE +W PD+VL N A
Sbjct: 91 GVDTLHVPSEHIWLPDIVLYNNA 113
>DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated
ion channel subunit protein.
Length = 510
Score = 27.1 bits (57), Expect = 0.21
Identities = 15/58 (25%), Positives = 24/58 (41%), Gaps = 3/58 (5%)
Frame = +3
Query: 351 ERLWRPDVVLLNAAATTAGDYAL---RARVSNXXSVSWIKRLDMSTPISXQLDNWPND 515
ER+WRPD N + + R+S + + RL + +L N+P D
Sbjct: 115 ERIWRPDTYFYNGKHSYVHTITVPNKLLRISQDGDILYSMRLTIKAKCPMELRNFPMD 172
>AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic
acetylcholine receptorApisa2 subunit protein.
Length = 541
Score = 27.1 bits (57), Expect = 0.21
Identities = 14/26 (53%), Positives = 17/26 (65%), Gaps = 1/26 (3%)
Frame = +3
Query: 318 EWGCSTWL-VSSERLWRPDVVLLNAA 392
E+G T L V SE +W PD+VL N A
Sbjct: 88 EYGGVTELYVPSEHIWLPDIVLYNNA 113
>AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic
acetylcholine receptoralpha-3 protein.
Length = 537
Score = 26.6 bits (56), Expect = 0.28
Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 4/48 (8%)
Frame = +3
Query: 324 GCSTWLVSSERLWRPDVVLLNAAATTAGDYAL----RARVSNXXSVSW 455
G V SE +W PD+VL N A G+Y + +A + VSW
Sbjct: 104 GVEMLYVPSENIWLPDIVLYNNA---DGNYEVTLMTKATLKYTGDVSW 148
>AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic
acetylcholine Apisa7-2 subunit protein.
Length = 461
Score = 25.8 bits (54), Expect = 0.50
Identities = 15/60 (25%), Positives = 24/60 (40%), Gaps = 2/60 (3%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAAATTAGDYALRAR--VSNXXSVSWIKRLDMSTPISXQLDNWPND 515
V R+WRPD +L N A + VS+ V W+ + ++ +P D
Sbjct: 82 VPYNRVWRPDTILYNNADPQYSSAVINTNVIVSHTGEVVWLSHGIFRSSCDIDVEFFPFD 141
>DQ026036-1|AAY87895.1| 529|Apis mellifera nicotinic acetylcholine
receptor alpha6subunit protein.
Length = 529
Score = 25.4 bits (53), Expect = 0.65
Identities = 15/67 (22%), Positives = 30/67 (44%), Gaps = 5/67 (7%)
Frame = +2
Query: 140 MIDSLLATYDRESPP----DSKIVVNLTLHLRHA-NIRESESTVRILADLQMNWIDKRLS 304
+++ LL++Y+ P + V + L+ ++ E + A L++ W+D L
Sbjct: 58 LLNELLSSYNTLERPVANESEPLEVKFGITLQQIIDVDEKNQILTTNAWLKLEWVDYNLQ 117
Query: 305 WNAGRMG 325
WN G
Sbjct: 118 WNESEYG 124
Score = 24.6 bits (51), Expect = 1.1
Identities = 6/17 (35%), Positives = 14/17 (82%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA 392
++ +LW+PD+++ N+A
Sbjct: 131 ITPNKLWKPDILMYNSA 147
>DQ026035-1|AAY87894.1| 529|Apis mellifera nicotinic acetylcholine
receptor alpha6subunit protein.
Length = 529
Score = 25.4 bits (53), Expect = 0.65
Identities = 15/67 (22%), Positives = 30/67 (44%), Gaps = 5/67 (7%)
Frame = +2
Query: 140 MIDSLLATYDRESPP----DSKIVVNLTLHLRHA-NIRESESTVRILADLQMNWIDKRLS 304
+++ LL++Y+ P + V + L+ ++ E + A L++ W+D L
Sbjct: 58 LLNELLSSYNTLERPVANESEPLEVKFGITLQQIIDVDEKNQILTTNAWLKLEWVDYNLQ 117
Query: 305 WNAGRMG 325
WN G
Sbjct: 118 WNESEYG 124
Score = 24.6 bits (51), Expect = 1.1
Identities = 6/17 (35%), Positives = 14/17 (82%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA 392
++ +LW+PD+++ N+A
Sbjct: 131 ITPNKLWKPDILMYNSA 147
>AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic
acetylcholine receptoralpha7-1 protein.
Length = 555
Score = 25.4 bits (53), Expect = 0.65
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA 392
+ RLW+PDV++ N+A
Sbjct: 100 IPPHRLWKPDVLMYNSA 116
>EF127805-1|ABL67942.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 6 protein.
Length = 461
Score = 25.0 bits (52), Expect = 0.86
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRILADLQMNWIDKRLSWNAGRMG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 24.6 bits (51), Expect = 1.1
Identities = 6/17 (35%), Positives = 14/17 (82%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA 392
++ +LW+PD+++ N+A
Sbjct: 63 ITPNKLWKPDILMYNSA 79
>EF127804-1|ABL67941.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 5 protein.
Length = 461
Score = 25.0 bits (52), Expect = 0.86
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRILADLQMNWIDKRLSWNAGRMG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 24.6 bits (51), Expect = 1.1
Identities = 6/17 (35%), Positives = 14/17 (82%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA 392
++ +LW+PD+++ N+A
Sbjct: 63 ITPNKLWKPDILMYNSA 79
>EF127803-1|ABL67940.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 4 protein.
Length = 461
Score = 25.0 bits (52), Expect = 0.86
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRILADLQMNWIDKRLSWNAGRMG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 24.6 bits (51), Expect = 1.1
Identities = 6/17 (35%), Positives = 14/17 (82%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA 392
++ +LW+PD+++ N+A
Sbjct: 63 ITPNKLWKPDILMYNSA 79
>EF127802-1|ABL67939.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 3 protein.
Length = 461
Score = 25.0 bits (52), Expect = 0.86
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRILADLQMNWIDKRLSWNAGRMG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 24.6 bits (51), Expect = 1.1
Identities = 6/17 (35%), Positives = 14/17 (82%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA 392
++ +LW+PD+++ N+A
Sbjct: 63 ITPNKLWKPDILMYNSA 79
>EF127801-1|ABL67938.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 2 protein.
Length = 461
Score = 25.0 bits (52), Expect = 0.86
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRILADLQMNWIDKRLSWNAGRMG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 24.6 bits (51), Expect = 1.1
Identities = 6/17 (35%), Positives = 14/17 (82%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA 392
++ +LW+PD+++ N+A
Sbjct: 63 ITPNKLWKPDILMYNSA 79
>EF127800-1|ABL67937.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 1 protein.
Length = 461
Score = 25.0 bits (52), Expect = 0.86
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRILADLQMNWIDKRLSWNAGRMG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 24.6 bits (51), Expect = 1.1
Identities = 6/17 (35%), Positives = 14/17 (82%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA 392
++ +LW+PD+++ N+A
Sbjct: 63 ITPNKLWKPDILMYNSA 79
>DQ667182-1|ABG75734.1| 445|Apis mellifera GABA-gated chloride
channel protein.
Length = 445
Score = 25.0 bits (52), Expect = 0.86
Identities = 18/78 (23%), Positives = 40/78 (51%), Gaps = 4/78 (5%)
Frame = +2
Query: 119 NATSLSLMIDSLLATYDRESPPD---SKIVVNLTLH-LRHANIRESESTVRILADLQMNW 286
N ++S ++DS +YD+ P+ + V +T++ L +++ E + + + W
Sbjct: 8 NDVNISAILDSFSVSYDKRVRPNYGGPPVEVGVTMYVLSISSLSEVKMDFTLDFYFRQFW 67
Query: 287 IDKRLSWNAGRMGLQHLA 340
D RL++ R G++ L+
Sbjct: 68 TDPRLAFKK-RTGVETLS 84
>DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholine
receptor beta1subunit protein.
Length = 520
Score = 25.0 bits (52), Expect = 0.86
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 351 ERLWRPDVVLLNAAATTAGDYALR 422
+++W+PD+VL N A G+Y +R
Sbjct: 106 DKVWKPDIVLFNNA---DGNYEVR 126
Score = 22.2 bits (45), Expect = 6.1
Identities = 12/46 (26%), Positives = 21/46 (45%), Gaps = 1/46 (2%)
Frame = +2
Query: 191 KIVVNLTL-HLRHANIRESESTVRILADLQMNWIDKRLSWNAGRMG 325
K+ VN L ++ N+ E ++ L+ W D +L W+ G
Sbjct: 51 KVHVNFGLAFVQLINVNEKNQIMKSNVWLRFIWTDYQLQWDEADYG 96
>DQ667181-1|ABG75733.1| 445|Apis mellifera GABA-gated chloride
channel protein.
Length = 445
Score = 23.8 bits (49), Expect = 2.0
Identities = 18/78 (23%), Positives = 39/78 (50%), Gaps = 4/78 (5%)
Frame = +2
Query: 119 NATSLSLMIDSLLATYDRESPPD---SKIVVNLTLH-LRHANIRESESTVRILADLQMNW 286
N ++S ++DS +YD+ P+ + V +T++ L +++ E + + W
Sbjct: 8 NDVNISAILDSFSVSYDKRVRPNYGGPPVEVGVTMYVLSISSVSEVLMDFTLDFYFRQFW 67
Query: 287 IDKRLSWNAGRMGLQHLA 340
D RL++ R G++ L+
Sbjct: 68 TDPRLAFKK-RTGVETLS 84
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 181,771
Number of Sequences: 438
Number of extensions: 4054
Number of successful extensions: 35
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 26
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 26824317
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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