BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP16_FL5_D04
(935 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 36 0.008
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 33 0.058
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.076
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.23
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 30 0.41
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 25 1.7
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 2.9
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 5.0
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 35.9 bits (79), Expect = 0.008
Identities = 15/43 (34%), Positives = 17/43 (39%)
Frame = +2
Query: 578 PPPPPPXKXXKTPPPXRRGXXAXXXXPPPPGXXXXXXXXXPXP 706
PPPPPP + + PP G PPPP P P
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLP 354
Score = 33.5 bits (73), Expect = 0.044
Identities = 16/44 (36%), Positives = 17/44 (38%), Gaps = 2/44 (4%)
Frame = +3
Query: 576 PPPPPPPXK--XXKPPPXRXXGXXRXXXPXXPPXRXXXGXXPXP 701
PPPPPPP + KPP P PP G P P
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLP 354
Score = 28.3 bits (60), Expect = 1.6
Identities = 12/27 (44%), Positives = 13/27 (48%), Gaps = 2/27 (7%)
Frame = +3
Query: 546 PXXPPGXGXXPPPPP--PPXKXXKPPP 620
P P G PPPPP P +PPP
Sbjct: 352 PLPPQGRSAPPPPPPRSAPSTGRQPPP 378
Score = 27.5 bits (58), Expect = 2.9
Identities = 24/101 (23%), Positives = 27/101 (26%)
Frame = +2
Query: 578 PPPPPPXKXXKTPPPXRRGXXAXXXXPPPPGXXXXXXXXXPXPTXXXGXXGRXXXGXXPX 757
PP PPP ++ P A PP P P+ G P
Sbjct: 388 PPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAP-PSLPPSAPPSLPMGA-PA 445
Query: 758 GXXXXPXXGCXPXAXTTXXXPXPXPPRXXXXPRAPXPXPXA 880
P P P PP P AP P P A
Sbjct: 446 APPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAA 486
Score = 27.1 bits (57), Expect = 3.8
Identities = 26/100 (26%), Positives = 28/100 (28%), Gaps = 1/100 (1%)
Frame = +2
Query: 578 PPPPPPXKXXK-TPPPXRRGXXAXXXXPPPPGXXXXXXXXXPXPTXXXGXXGRXXXGXXP 754
PPPPPP + P +G A PPPP P P
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSA---PPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPA 394
Query: 755 XGXXXXPXXGCXPXAXTTXXXPXPXPPRXXXXPRAPXPXP 874
P A T P P PP P AP P
Sbjct: 395 IPGRSAPALPPLGNASRTSTPPVPTPP--SLPPSAPPSLP 432
Score = 26.6 bits (56), Expect = 5.0
Identities = 26/107 (24%), Positives = 27/107 (25%)
Frame = +2
Query: 554 PPGXRGXXPPPPPPXKXXKTPPPXRRGXXAXXXXPPPPGXXXXXXXXXPXPTXXXGXXGR 733
PP P PP PPP R + PPP P GR
Sbjct: 342 PPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPP---LSSSRAVSNPPAPPPAIPGR 398
Query: 734 XXXGXXPXGXXXXPXXGCXPXAXTTXXXPXPXPPRXXXXPRAPXPXP 874
P G P T P PP P AP P
Sbjct: 399 SAPALPPLGNASRT---STPPVPTPPSLPPSAPP--SLPPSAPPSLP 440
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 33.1 bits (72), Expect = 0.058
Identities = 15/25 (60%), Positives = 15/25 (60%)
Frame = -2
Query: 619 GGGFXXFXGGGGGGGXXPXPGGXXG 545
GGGF F GGG GG P PGG G
Sbjct: 187 GGGFGGF--GGGSGGPPPGPGGFGG 209
Score = 26.2 bits (55), Expect = 6.6
Identities = 13/25 (52%), Positives = 13/25 (52%)
Frame = -2
Query: 619 GGGFXXFXGGGGGGGXXPXPGGXXG 545
GGG F GGG GG PGG G
Sbjct: 248 GGGLGGF--GGGPGGFGGGPGGHGG 270
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 32.7 bits (71), Expect = 0.076
Identities = 16/37 (43%), Positives = 16/37 (43%)
Frame = +3
Query: 546 PXXPPGXGXXPPPPPPPXKXXKPPPXRXXGXXRXXXP 656
P PPG PPPPPP PPP G R P
Sbjct: 764 PPPPPGVAGAGPPPPPP-----PPPAVSAGGSRYYAP 795
Score = 31.1 bits (67), Expect = 0.23
Identities = 15/37 (40%), Positives = 16/37 (43%)
Frame = +2
Query: 554 PPGXRGXXPPPPPPXKXXKTPPPXRRGXXAXXXXPPP 664
PPG G PPPPPP PPP + P P
Sbjct: 767 PPGVAGAGPPPPPP------PPPAVSAGGSRYYAPAP 797
Score = 30.3 bits (65), Expect = 0.41
Identities = 13/37 (35%), Positives = 13/37 (35%)
Frame = +3
Query: 558 PGXGXXPPPPPPPXKXXKPPPXRXXGXXRXXXPXXPP 668
P P PPP P PPP G P PP
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 30.3 bits (65), Expect = 0.41
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = +3
Query: 546 PXXPPGXGXXPPPPPPPXKXXKPPP 620
P P G PPPPPPP PP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPP 776
Score = 29.9 bits (64), Expect = 0.54
Identities = 19/66 (28%), Positives = 19/66 (28%), Gaps = 7/66 (10%)
Frame = +2
Query: 578 PPPPPPXKXXKTP-------PPXRRGXXAXXXXPPPPGXXXXXXXXXPXPTXXXGXXGRX 736
PPPPPP TP PP PPPPG P P G
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSR 791
Query: 737 XXGXXP 754
P
Sbjct: 792 YYAPAP 797
Score = 29.9 bits (64), Expect = 0.54
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = +3
Query: 546 PXXPPGXGXXPPPPPPPXKXXKPPP 620
P P G PPPPPP PPP
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPP 777
Score = 27.1 bits (57), Expect = 3.8
Identities = 15/46 (32%), Positives = 15/46 (32%), Gaps = 4/46 (8%)
Frame = +3
Query: 576 PPPPPP----PXKXXKPPPXRXXGXXRXXXPXXPPXRXXXGXXPXP 701
PPPPPP P P P P PP G P P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
Score = 25.8 bits (54), Expect = 8.8
Identities = 17/52 (32%), Positives = 17/52 (32%)
Frame = +3
Query: 546 PXXPPGXGXXPPPPPPPXKXXKPPPXRXXGXXRXXXPXXPPXRXXXGXXPXP 701
P PP P P P P PPP G P PP G P P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPV--PPPAPIMGG--PPPPPPPPGVAGAGPPPPP 779
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 31.1 bits (67), Expect = 0.23
Identities = 13/25 (52%), Positives = 14/25 (56%), Gaps = 3/25 (12%)
Frame = +3
Query: 555 PPGXGXXPPPPP---PPXKXXKPPP 620
PPG PPPPP PP + PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPP 29
Score = 27.9 bits (59), Expect = 2.2
Identities = 13/29 (44%), Positives = 14/29 (48%), Gaps = 3/29 (10%)
Frame = +2
Query: 545 ARXPPGXRGXXPPPP---PPXKXXKTPPP 622
A PPG PPPP PP + PPP
Sbjct: 2 ASLPPGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 30.3 bits (65), Expect = 0.41
Identities = 13/31 (41%), Positives = 13/31 (41%)
Frame = +2
Query: 578 PPPPPPXKXXKTPPPXRRGXXAXXXXPPPPG 670
PPPPPP K PP A PP G
Sbjct: 1882 PPPPPPMALPKAGPPSAAPTSALPPAGPPAG 1912
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 25.0 bits (52), Expect(2) = 1.7
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +3
Query: 576 PPPPPPPXKXXK 611
PPPPPPP + K
Sbjct: 1096 PPPPPPPAEVEK 1107
Score = 21.4 bits (43), Expect(2) = 1.7
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = +3
Query: 558 PGXGXXPPPPPP 593
P PPPPPP
Sbjct: 1091 PSAVPPPPPPPP 1102
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.5 bits (58), Expect = 2.9
Identities = 16/51 (31%), Positives = 16/51 (31%)
Frame = +2
Query: 554 PPGXRGXXPPPPPPXKXXKTPPPXRRGXXAXXXXPPPPGXXXXXXXXXPXP 706
PP PPPPP PPP A PPP P P
Sbjct: 1699 PPQMSAPTPPPPP---MSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 26.6 bits (56), Expect = 5.0
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +3
Query: 576 PPPPPPPXKXXKPPPXRXXG 635
PPPPPPP P R G
Sbjct: 371 PPPPPPPELLNHSPKSRPLG 390
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,537,046
Number of Sequences: 5004
Number of extensions: 19675
Number of successful extensions: 303
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 52
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 475330268
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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