Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP15_FL5_J18
(805 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 280 2e-76
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 272 3e-74
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 142 7e-35
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 121 1e-28
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 27 2.4
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.2
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.2
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.5
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 280 bits (686), Expect = 2e-76
Identities = 124/183 (67%), Positives = 149/183 (81%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 286 HVPRAVFVDLEPTVVDGVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPR+++VDLEP V+D VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 645
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 646 VVE 654
VVE
Sbjct: 181 VVE 183
Score = 73.7 bits (173), Expect = 3e-14
Identities = 30/40 (75%), Positives = 35/40 (87%)
Frame = +3
Query: 657 YNSILTTHTXLEHSDCAFMVDNEAIYDICRRNLDIERPTY 776
YNS+LTTH L+ +DC FMVDNE+ YDICRRNLDIERP+Y
Sbjct: 185 YNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSY 224
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 272 bits (668), Expect = 3e-74
Identities = 125/188 (66%), Positives = 151/188 (80%), Gaps = 5/188 (2%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 271 TGAGKHVPRAVFVDLEPTVVDGVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 450
TG GK VPR+++VDLEP V+D VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 451 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 630
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 631 QVSTAVVE 654
QVST+VVE
Sbjct: 180 QVSTSVVE 187
Score = 79.4 bits (187), Expect = 6e-16
Identities = 33/40 (82%), Positives = 36/40 (90%)
Frame = +3
Query: 657 YNSILTTHTXLEHSDCAFMVDNEAIYDICRRNLDIERPTY 776
YNS+LTTH L++SDC FMVDNEA YDICRRNLDIERPTY
Sbjct: 189 YNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTY 228
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 142 bits (343), Expect = 7e-35
Identities = 70/183 (38%), Positives = 100/183 (54%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 286 HVPRAVFVDLEPTVVDGVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 645
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 646 VVE 654
VVE
Sbjct: 179 VVE 181
Score = 39.5 bits (88), Expect = 5e-04
Identities = 17/40 (42%), Positives = 25/40 (62%)
Frame = +3
Query: 657 YNSILTTHTXLEHSDCAFMVDNEAIYDICRRNLDIERPTY 776
YN+ L+ H +E+SD F +DNEA+ I L I+ P+Y
Sbjct: 183 YNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSY 222
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 121 bits (291), Expect = 1e-28
Identities = 67/185 (36%), Positives = 104/185 (56%), Gaps = 3/185 (1%)
Frame = +1
Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 289 VPRAVFVDLEPTVVDGVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 462
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 463 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 639
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 640 TAVVE 654
VV+
Sbjct: 180 DVVVQ 184
Score = 27.1 bits (57), Expect = 3.1
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +3
Query: 657 YNSILTTHTXLEHSDCAFMVDNEAIYDICRRNLDIERPTY 776
YNS+L ++D ++DN A+ I L + PT+
Sbjct: 186 YNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTF 225
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 27.5 bits (58), Expect = 2.4
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = -3
Query: 365 NNCLYVPVRTPSTTVGSRSTKTARGTCLPAPVSLKKVLKESSPP 234
N+ +Y PV + STT S ST + T A S + +S P
Sbjct: 740 NDAVYYPVSSSSTTTTSTSTGSVTTTSTTATASCTLPISYTSTP 783
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.2
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 185 STASSLMARCPQTRPSGVETILSTLSSARPELAST 289
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.5
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 95 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 217
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,365,098
Number of Sequences: 5004
Number of extensions: 70683
Number of successful extensions: 238
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 234
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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