Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP15_FL5_D05
(856 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB8E5.06c |rpl302|rpl3-2, rpl3-b|60S ribosomal protein L3|Sch... 192 4e-50
SPAC17A5.03 |rpl301|rpl3-1, rpl3|60S ribosomal protein L3 |Schiz... 192 5e-50
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 27 3.4
SPBC1683.12 |||nicotinic acid plasma membrane transporter |Schiz... 26 5.9
SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFII... 26 5.9
>SPAPB8E5.06c |rpl302|rpl3-2, rpl3-b|60S ribosomal protein
L3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 388
Score = 192 bits (469), Expect = 4e-50
Identities = 89/158 (56%), Positives = 117/158 (74%)
Frame = +2
Query: 176 GYKAGMTHVVREPDRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWA 355
GYKAGMTH+VR+ DRPGSK++K+EI+EAVTIIETPPMV VGVVGY+ETP GLR+L TVWA
Sbjct: 48 GYKAGMTHIVRDLDRPGSKMHKREILEAVTIIETPPMVVVGVVGYVETPRGLRSLTTVWA 107
Query: 356 EHMSEDCRRRXXXXXXXXXXXXXXXXXXXXQDELGRKSIEKDFKKMIRYCSVVRVIAHTQ 535
EH+SE+ +RR + +SI ++ +++ +YCSVVRV+AHTQ
Sbjct: 108 EHLSEEVKRRFYKNWFKSKKKAFTKYAKKYAE--STQSINRELERIKKYCSVVRVLAHTQ 165
Query: 536 MKLLKQRQKKAHIMEIQLNGGTIEDKVKWAREHLEKPI 649
++ QKKAH+MEIQ+NGG++ DKV+WAREH EK +
Sbjct: 166 IRKTPLAQKKAHLMEIQVNGGSVADKVEWAREHFEKTV 203
Score = 83.4 bits (197), Expect = 4e-17
Identities = 37/67 (55%), Positives = 44/67 (65%)
Frame = +3
Query: 654 VDSVFXQDEMIDCIXXXXXXXXXXXXSRWHTKKLPRKTHKGLRKVACIGXWHPSRVSFTV 833
+ S F Q+EMID I +RW TK+LPRKTH+GLRKVACIG WHP+ V +TV
Sbjct: 205 IKSTFEQNEMIDVIGVTRGKGNEGTTARWGTKRLPRKTHRGLRKVACIGAWHPANVQWTV 264
Query: 834 XRAGQKG 854
RAG G
Sbjct: 265 ARAGNAG 271
Score = 77.4 bits (182), Expect = 2e-15
Identities = 32/43 (74%), Positives = 37/43 (86%)
Frame = +1
Query: 49 FQHPXHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFIG 177
F+ P HGS+GF P+KR+ R RGKVKAFPKDD SKPVHLTAF+G
Sbjct: 6 FEQPRHGSLGFLPRKRASRQRGKVKAFPKDDASKPVHLTAFLG 48
>SPAC17A5.03 |rpl301|rpl3-1, rpl3|60S ribosomal protein L3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 388
Score = 192 bits (468), Expect = 5e-50
Identities = 88/158 (55%), Positives = 117/158 (74%)
Frame = +2
Query: 176 GYKAGMTHVVREPDRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWA 355
GYKAGMTH+VR+ DRPGSK++K+EI+EAVT+IETPPMV VGVVGY+ETP GLR+L TVWA
Sbjct: 48 GYKAGMTHIVRDLDRPGSKMHKREILEAVTVIETPPMVVVGVVGYVETPRGLRSLTTVWA 107
Query: 356 EHMSEDCRRRXXXXXXXXXXXXXXXXXXXXQDELGRKSIEKDFKKMIRYCSVVRVIAHTQ 535
EH+SE+ +RR + +SI ++ +++ +YCSVVRV+AHTQ
Sbjct: 108 EHLSEEVKRRFYKNWFKSKKKAFTKYAKKYAE--STQSINRELERIKKYCSVVRVLAHTQ 165
Query: 536 MKLLKQRQKKAHIMEIQLNGGTIEDKVKWAREHLEKPI 649
++ QKKAH+MEIQ+NGG++ DKV+WAREH EK +
Sbjct: 166 IRKTPLAQKKAHLMEIQVNGGSVADKVEWAREHFEKTV 203
Score = 83.4 bits (197), Expect = 4e-17
Identities = 37/67 (55%), Positives = 44/67 (65%)
Frame = +3
Query: 654 VDSVFXQDEMIDCIXXXXXXXXXXXXSRWHTKKLPRKTHKGLRKVACIGXWHPSRVSFTV 833
+ S F Q+EMID I +RW TK+LPRKTH+GLRKVACIG WHP+ V +TV
Sbjct: 205 IKSTFEQNEMIDVIGVTRGKGNEGTTARWGTKRLPRKTHRGLRKVACIGAWHPANVQWTV 264
Query: 834 XRAGQKG 854
RAG G
Sbjct: 265 ARAGNAG 271
Score = 77.4 bits (182), Expect = 2e-15
Identities = 32/43 (74%), Positives = 37/43 (86%)
Frame = +1
Query: 49 FQHPXHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFIG 177
F+ P HGS+GF P+KR+ R RGKVKAFPKDD SKPVHLTAF+G
Sbjct: 6 FEQPRHGSLGFLPRKRASRQRGKVKAFPKDDASKPVHLTAFLG 48
>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
Shk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 658
Score = 27.1 bits (57), Expect = 3.4
Identities = 12/42 (28%), Positives = 20/42 (47%), Gaps = 2/42 (4%)
Frame = +1
Query: 37 RTENFQHPXH--GSMGFYPKKRSRRHRGKVKAFPKDDPSKPV 156
++E QHP +M FY + + G FP++ KP+
Sbjct: 186 KSEQVQHPQAVLDAMAFYSQSKKYLEEGAKPPFPRESTEKPL 227
>SPBC1683.12 |||nicotinic acid plasma membrane transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 482
Score = 26.2 bits (55), Expect = 5.9
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +2
Query: 503 CSVVRVIAHTQMKLLKQRQKKAHIMEIQLNGGTIEDK 613
C+VV + T M+ QRQ K I + LNG +++K
Sbjct: 433 CTVVGTLTATAMRFYLQRQNK--IKQEILNGERVDEK 467
>SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFIID
complex subunit Taf111|Schizosaccharomyces pombe|chr
1|||Manual
Length = 979
Score = 26.2 bits (55), Expect = 5.9
Identities = 18/51 (35%), Positives = 25/51 (49%)
Frame = +3
Query: 309 TLRPLMDYALC*LSGRSICLKTVDVASTKTGTNARRRLSLKPVRNGRMSLD 461
TL DYA+ S +S+ LK V+ T T RRR + RN + L+
Sbjct: 219 TLEVDTDYAIIFNSKKSLPLKRNVVSPISTHTKKRRRTANTSQRNDGLDLN 269
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,521,536
Number of Sequences: 5004
Number of extensions: 71469
Number of successful extensions: 165
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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