Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP14_T7_L09
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 31 0.13
SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity... 31 0.18
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 29 0.94
SPBC4B4.07c |usp102|mud1|U1 snRNP-associated protein Usp102|Schi... 29 0.94
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 28 1.6
SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 27 2.9
SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schiz... 27 3.8
SPBC1703.12 |ubp9||ubiquitin C-terminal hydrolase Ubp9|Schizosac... 26 5.0
SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 26 6.6
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 26 6.6
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 25 8.7
>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 422
Score = 31.5 bits (68), Expect = 0.13
Identities = 15/66 (22%), Positives = 32/66 (48%)
Frame = -2
Query: 524 PSKVLHFFNTPPDVSDEQLLQVFKDYGVTPPHTISKFPLKSERSSSGLMEFQNISQSVMA 345
PS V++ N P ++++EQ++ +FK G + P + G E+ + + + A
Sbjct: 3 PSCVVYVGNIPYEMAEEQVIDIFKQSGPVKSFQLVIDPESGQPKGYGFCEYHDPATAASA 62
Query: 344 IMACNH 327
+ N+
Sbjct: 63 VRNLNN 68
>SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ctf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 363
Score = 31.1 bits (67), Expect = 0.18
Identities = 15/61 (24%), Positives = 27/61 (44%)
Frame = -2
Query: 500 NTPPDVSDEQLLQVFKDYGVTPPHTISKFPLKSERSSSGLMEFQNISQSVMAIMACNHAT 321
N P DVS++Q+ ++F G + P G EF + + MA+ N++
Sbjct: 13 NIPYDVSEQQMTEIFNQVGPVKTFKLVLDPETGSGKGYGFCEFFDSETTAMAVRKLNNSE 72
Query: 320 I 318
+
Sbjct: 73 L 73
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 28.7 bits (61), Expect = 0.94
Identities = 24/84 (28%), Positives = 37/84 (44%), Gaps = 3/84 (3%)
Frame = -2
Query: 563 LTPASIHKNRIQPPSKVLHFFNTPP--DVSDEQLLQVFKDYGVTPPHTIS-KFPLKSERS 393
LTP HKN+ HFF+ P VS + L+ G++ +TIS F + +
Sbjct: 177 LTPQETHKNKSFERDDQKHFFDRPAFLTVSTQLHLEALA-LGLSRVYTISPAFRAEQSHT 235
Query: 392 SSGLMEFQNISQSVMAIMACNHAT 321
S L EF + V + + + T
Sbjct: 236 SRHLAEFWMLEAEVAFMTSLSQLT 259
>SPBC4B4.07c |usp102|mud1|U1 snRNP-associated protein
Usp102|Schizosaccharomyces pombe|chr 2|||Manual
Length = 249
Score = 28.7 bits (61), Expect = 0.94
Identities = 16/53 (30%), Positives = 31/53 (58%)
Frame = -2
Query: 605 PSFKDYVGNKNNRFLTPASIHKNRIQPPSKVLHFFNTPPDVSDEQLLQVFKDY 447
P+ K V +N ++I+++ + PP+KVL N P +V+ + L Q+F+ +
Sbjct: 146 PTHKKPVPKRNVGAERKSTINEDLL-PPNKVLLLQNIPQEVNADVLTQIFEAF 197
>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1327
Score = 27.9 bits (59), Expect = 1.6
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +3
Query: 528 LYSVLVYRGRR*EAIVLVTHVIFERRTLVGKRI 626
L +V+ + RR AI L V FER T VGK +
Sbjct: 626 LQTVVCTQPRRISAISLAERVAFERDTTVGKEV 658
>SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 561
Score = 27.1 bits (57), Expect = 2.9
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = -2
Query: 560 TPASIHKNRIQPPSKVLHFFNTPPDVSDEQLLQVF 456
TP + + PP ++ N PP S+E+L +F
Sbjct: 412 TPTNTNPADQNPPCNTIYVGNLPPSTSEEELKVLF 446
>SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 900
Score = 26.6 bits (56), Expect = 3.8
Identities = 16/51 (31%), Positives = 26/51 (50%)
Frame = -2
Query: 683 YTLTLAFSKQAYLSEVMNPYPLPDKSPSFKDYVGNKNNRFLTPASIHKNRI 531
YTL L SK+ ++MNP +P +DY N++ + P ++ N I
Sbjct: 265 YTLALKTSKRIIYLDIMNPV-----NPDIQDYEFNESPKLSVP-TVEMNMI 309
>SPBC1703.12 |ubp9||ubiquitin C-terminal hydrolase
Ubp9|Schizosaccharomyces pombe|chr 2|||Manual
Length = 585
Score = 26.2 bits (55), Expect = 5.0
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -2
Query: 635 MNPYPLPDKSPSFKDYVGNKNNRFLTPASIHKNR 534
+N YPLP P+FK V K N + +S H +
Sbjct: 69 LNSYPLPSAPPNFKS-VCTKTNHPESSSSRHSKK 101
>SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1372
Score = 25.8 bits (54), Expect = 6.6
Identities = 23/91 (25%), Positives = 42/91 (46%), Gaps = 7/91 (7%)
Frame = -2
Query: 701 NVTVGD-YTLTLAFSKQAYLSEVMNPYPLPDKSPS---FKDYVG--NKNNRFLTPASIHK 540
N+ V D Y LTL + + ++ + YPL + + S K+Y G + + + +H+
Sbjct: 896 NIVVADPYLLTLKLTAEGIFKQLEDCYPLREVTVSNKKVKEYTGFMPVDLQQMVSIRLHR 955
Query: 539 -NRIQPPSKVLHFFNTPPDVSDEQLLQVFKD 450
N + P FF+ D+ +L+ F D
Sbjct: 956 FNSLTPIEIAKKFFHCDHDIM--ELVDFFND 984
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 25.8 bits (54), Expect = 6.6
Identities = 22/114 (19%), Positives = 48/114 (42%), Gaps = 6/114 (5%)
Frame = -2
Query: 635 MNPYPLPDKSPSFKDYVGNKNNRFLTPASIHKNRIQPPSKVLHFFNTPPD----VSD--E 474
M+P P+P F ++ +L+P S ++N + S+ + P +S+ +
Sbjct: 270 MSP-PIPPTPSQFSAFMYQNQQSYLSPQSHYENPLSISSRPSPCCPSTPSSAVTLSNGFD 328
Query: 473 QLLQVFKDYGVTPPHTISKFPLKSERSSSGLMEFQNISQSVMAIMACNHATIQH 312
+ + G PP + KFP +S + + Q S+ ++ + ++H
Sbjct: 329 SQSNAYNNSGTGPP-MLYKFPQRSYTAPNTSFNSQRRMSSITSLPTASFCPVKH 381
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 25.4 bits (53), Expect = 8.7
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -2
Query: 515 VLHFFNTPPDVSDEQLLQ 462
VLHFF T DV D++L +
Sbjct: 819 VLHFFGTMTDVHDQKLAE 836
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,904,727
Number of Sequences: 5004
Number of extensions: 57201
Number of successful extensions: 152
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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