Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP14_FL5_N07
(872 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 312 3e-86
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 309 3e-85
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 148 1e-36
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 122 7e-29
SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat protei... 29 0.86
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 312 bits (767), Expect = 3e-86
Identities = 145/225 (64%), Positives = 175/225 (77%), Gaps = 4/225 (1%)
Frame = +2
Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 283
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 284 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 463
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 464 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVPL- 640
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQV
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 641 ---PSSSLQLYPHHPXNLEHSDCAFMVDNEAIYDICRRNLDIERP 766
P +S+ L H L+ +DC FMVDNE+ YDICRRNLDIERP
Sbjct: 181 VVEPYNSV-LTTH--ATLDLADCTFMVDNESCYDICRRNLDIERP 222
Score = 34.3 bits (75), Expect = 0.023
Identities = 15/22 (68%), Positives = 18/22 (81%)
Frame = +1
Query: 763 PTYTNLNRLIGXIVSSITXSLK 828
P+Y NLNRLI +VSSIT SL+
Sbjct: 222 PSYENLNRLIAQVVSSITASLR 243
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 309 bits (759), Expect = 3e-85
Identities = 148/230 (64%), Positives = 178/230 (77%), Gaps = 9/230 (3%)
Frame = +2
Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 268
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 269 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 448
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 449 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 628
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 629 QVPL----PSSSLQLYPHHPXNLEHSDCAFMVDNEAIYDICRRNLDIERP 766
QV P +S+ L H L++SDC FMVDNEA YDICRRNLDIERP
Sbjct: 180 QVSTSVVEPYNSV-LTTH--ATLDNSDCTFMVDNEACYDICRRNLDIERP 226
Score = 35.9 bits (79), Expect = 0.008
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = +1
Query: 763 PTYTNLNRLIGXIVSSITXSLK 828
PTY NLNRLI +VSSIT SL+
Sbjct: 226 PTYENLNRLIAQVVSSITASLR 247
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 148 bits (358), Expect = 1e-36
Identities = 81/226 (35%), Positives = 119/226 (52%), Gaps = 5/226 (2%)
Frame = +2
Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 283
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 284 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 463
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 464 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVPLP 643
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK---- 174
Query: 644 SSSLQLYPHHP-----XNLEHSDCAFMVDNEAIYDICRRNLDIERP 766
SS + P++ +E+SD F +DNEA+ I L I+ P
Sbjct: 175 SSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSP 220
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 122 bits (294), Expect = 7e-29
Identities = 75/227 (33%), Positives = 117/227 (51%), Gaps = 7/227 (3%)
Frame = +2
Query: 107 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 286
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 287 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 460
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 461 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ--- 631
D I + AD L+GF + H L+ERL+ Y KK ++++P Q
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 632 --VPLPSSSLQLYPHHPXNLEHSDCAFMVDNEAIYDICRRNLDIERP 766
V P +SL N +D ++DN A+ I L + P
Sbjct: 180 DVVVQPYNSLLALKRLTLN---ADSVVVLDNAALAHIAADRLHTQNP 223
>SPAC23H4.01c ||SPAP27G11.01|sterol binding ankyrin repeat
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 945
Score = 29.1 bits (62), Expect = 0.86
Identities = 20/56 (35%), Positives = 24/56 (42%), Gaps = 4/56 (7%)
Frame = +2
Query: 620 PAPQVPLPSSSLQLY--PHHPXNLEHSDCAFMVDNEAIYDICRRNL--DIERPPTP 775
P P P PS S LY PH + A V +I I R+N+ DI P P
Sbjct: 536 PKPLTPKPSDSNSLYPLPHSKVGRRKNIPAITVPPPSILSILRKNIGKDISSIPAP 591
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.1
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 183 STASSLMARCPQTRPSGVETILSTLSSARPELAST 287
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 202 WPDAHRQDHRGWRRFFQHFLQR 267
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,459,960
Number of Sequences: 5004
Number of extensions: 72245
Number of successful extensions: 253
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 247
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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