Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP14_FL5_G17
(862 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 392 e-110
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 390 e-109
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 198 1e-51
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 157 2e-39
SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo... 28 1.5
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 2.6
SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr... 26 7.9
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.9
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 26 7.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.9
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 392 bits (966), Expect = e-110
Identities = 175/257 (68%), Positives = 210/257 (81%)
Frame = +1
Query: 31 VHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAV 210
+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+VPR++
Sbjct: 7 IHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSI 66
Query: 211 FVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYVRGHYTIVKEIVDLVLDRIRKLA 390
+VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNY RGHYT+ KE+VD V D+IR++A
Sbjct: 67 YVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIA 126
Query: 391 DQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYS 570
D C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPY+
Sbjct: 127 DNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYN 186
Query: 571 SILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDG 750
S+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G
Sbjct: 187 SVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEG 246
Query: 751 ALKCGPHRVQTNLVPYP 801
+L QTNLVPYP
Sbjct: 247 SLNVDLAEFQTNLVPYP 263
Score = 39.5 bits (88), Expect = 6e-04
Identities = 21/35 (60%), Positives = 24/35 (68%), Gaps = 3/35 (8%)
Frame = +2
Query: 758 NVDLTEFRLTWCLTPY---HXPLVTYAPVISAEKA 853
NVDL EF+ L PY H PLVTYAP++SA KA
Sbjct: 249 NVDLAEFQTN--LVPYPRIHFPLVTYAPIVSAAKA 281
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 390 bits (961), Expect = e-109
Identities = 179/262 (68%), Positives = 213/262 (81%), Gaps = 5/262 (1%)
Frame = +1
Query: 31 VHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKH 195
VHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSETG GK
Sbjct: 7 VHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKF 65
Query: 196 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYVRGHYTIVKEIVDLVLDR 375
VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNY RGHYT+ KE++D VL+R
Sbjct: 66 VPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLER 125
Query: 376 IRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAV 555
IR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST+V
Sbjct: 126 IRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSV 185
Query: 556 VEPYSSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITAS 735
VEPY+S+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITAS
Sbjct: 186 VEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITAS 245
Query: 736 LRFDGALKCGPHRVQTNLVPYP 801
LRF G+L + QTNLVPYP
Sbjct: 246 LRFAGSLNVDLNEFQTNLVPYP 267
Score = 38.3 bits (85), Expect = 0.001
Identities = 20/35 (57%), Positives = 24/35 (68%), Gaps = 3/35 (8%)
Frame = +2
Query: 758 NVDLTEFRLTWCLTPY---HXPLVTYAPVISAEKA 853
NVDL EF+ L PY H PLVTY+P++SA KA
Sbjct: 253 NVDLNEFQTN--LVPYPRIHFPLVTYSPIVSAAKA 285
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 198 bits (482), Expect = 1e-51
Identities = 97/260 (37%), Positives = 146/260 (56%), Gaps = 2/260 (0%)
Frame = +1
Query: 28 LVHV--GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVP 201
+VH+ GQ G Q+G A W EHG+ G T + N +F+E GK+VP
Sbjct: 4 IVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVP 61
Query: 202 RAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYVRGHYTIVKEIVDLVLDRIR 381
RAV VDLEP +D V++G + LF P+ +I G+ A N + +GHYT E+ D VLD +R
Sbjct: 62 RAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVR 121
Query: 382 KLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVE 561
+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S VVE
Sbjct: 122 REAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVE 181
Query: 562 PYSSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLR 741
PY++ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T S R
Sbjct: 182 PYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFR 241
Query: 742 FDGALKCGPHRVQTNLVPYP 801
F G L ++ N+VP+P
Sbjct: 242 FPGELNSDLRKLAVNMVPFP 261
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 157 bits (380), Expect = 2e-39
Identities = 85/260 (32%), Positives = 141/260 (54%), Gaps = 3/260 (1%)
Frame = +1
Query: 31 VHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAV 210
+ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +++PRA+
Sbjct: 8 LQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAI 65
Query: 211 FVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYVRGHYTIVKEIVDLVLDRIRK 384
+DLEP VV+ + + TY L++PE ++ K A NN+ G Y+ + I + ++D I +
Sbjct: 66 LIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDR 124
Query: 385 LADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVE 561
AD L+GF + H L+ERL+ Y KK ++++P Q VS VV+
Sbjct: 125 EADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQ 184
Query: 562 PYSSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLR 741
PY+S+L ++D ++DN A+ I L + PT+ N+L+ ++S+ T +LR
Sbjct: 185 PYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLR 244
Query: 742 FDGALKCGPHRVQTNLVPYP 801
+ G + + +L+P P
Sbjct: 245 YPGYMNNDLVSIIASLIPSP 264
>SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase
Wis4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1401
Score = 28.3 bits (60), Expect = 1.5
Identities = 34/122 (27%), Positives = 54/122 (44%), Gaps = 7/122 (5%)
Frame = -2
Query: 417 ESLQTGTLVSELADSVQNQIYD-------FLYNGVVTTDIVVGRIFLTSNKLFWMKQLSV 259
ES+Q TLV L V N +D F Y+GV T V RIF S +Q ++
Sbjct: 376 ESIQVETLV--LQKWVGNDEFDLTMRTPQFNYDGVENTSSFVERIFRQSGLQRTFEQRTL 433
Query: 258 CASADLINNSRFKIDEDSTGYVLASSGLAEESVERIVSTPDGLVCGHLAIRLDAVLQAVK 79
+I+ ++ I E++ + E+ + +V P L+ L +RL A + +K
Sbjct: 434 TTLNRIIHQAKQTISENAQAFEEMKLPTYEDKLLPLVRFPIKLLEEALRLRL-AYAKKIK 492
Query: 78 LP 73
P
Sbjct: 493 GP 494
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 27.5 bits (58), Expect = 2.6
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = -2
Query: 744 ESQRSSDRGDNLSNETIQVGVGWALNVEITXADVIDG 634
+ Q+SSD ++ ++ T VG+G AL I D +DG
Sbjct: 182 DKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 703
Score = 25.8 bits (54), Expect = 7.9
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -2
Query: 786 VSLNSVRSTFQSAVESQRSSDRGDNLSNETIQ 691
+SLNS +TF + + S +S GD T+Q
Sbjct: 456 LSLNSTLNTFMNELNSSMTSAFGDTFLASTVQ 487
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.9
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 92 STASSLMARCPQTRPSGVETILSTLSSARPELAST 196
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 25.8 bits (54), Expect = 7.9
Identities = 16/68 (23%), Positives = 31/68 (45%)
Frame = -2
Query: 444 TSTEGVEDQESLQTGTLVSELADSVQNQIYDFLYNGVVTTDIVVGRIFLTSNKLFWMKQL 265
TS G+ + T L S L ++V+N+ N I++G++F + ++ +
Sbjct: 1329 TSLNGLRTDSADSTDALNSNLNNTVENEANQTALNYARNLLIIIGQLFQLAAQMPYTSMQ 1388
Query: 264 SVCASADL 241
V A +L
Sbjct: 1389 EVPADHEL 1396
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 111 WPDAHRQDHRGWRRFFQHFLQR 176
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,562,027
Number of Sequences: 5004
Number of extensions: 74300
Number of successful extensions: 257
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 243
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 252
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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