BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP14_FL5_F12
(875 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F7.04 |rho1||Rho family GTPase Rho1|Schizosaccharomyces pom... 30 0.38
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 29 0.66
SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|c... 29 0.87
SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 27 3.5
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 27 4.6
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 6.1
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 26 8.1
>SPAC1F7.04 |rho1||Rho family GTPase Rho1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 202
Score = 30.3 bits (65), Expect = 0.38
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +2
Query: 245 RDVFEHVRSAAKIKHYADQNTSSGTPQEREC 337
R+VFE AA +KH SSGT +++ C
Sbjct: 169 REVFESATRAAMLKHKPKVKPSSGTKKKKRC 199
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 29.5 bits (63), Expect = 0.66
Identities = 22/88 (25%), Positives = 44/88 (50%), Gaps = 1/88 (1%)
Frame = +2
Query: 179 FNKIMSTLKLTSEVRSGLYSHWRDVFEHVRSAAKIKHYADQNTSSGTPQEREC-SIRKSC 355
F+K++ST+ ++E+ + L WRD +HV + + +S C
Sbjct: 384 FSKMLSTI--SNEI-TNLQGDWRDQLDHVEFLQEHLGPLEVELASVKVLYDNCFQAGIEE 440
Query: 356 NNVSVTSMEDLSHEEIVTSYVLAHVAQF 439
N+ ++ S EDL HE +T+ ++A+ ++
Sbjct: 441 NDYTMFSYEDLEHEFGITANIIANKIKY 468
>SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1142
Score = 29.1 bits (62), Expect = 0.87
Identities = 18/59 (30%), Positives = 28/59 (47%)
Frame = -3
Query: 741 ACLLEPEVIERVVLLVLNRVDSKISATIHSLMSWFTVLVPYNSSSSHWLKNDSCRNYPL 565
AC L+P++ + L+ N + I+A H L T+L + +S DS NY L
Sbjct: 507 ACRLDPKIDNTISALLENGNNKVINANCHLLRELVTLLPASTAYTSSPYGGDSFYNYVL 565
>SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2812
Score = 27.1 bits (57), Expect = 3.5
Identities = 14/42 (33%), Positives = 19/42 (45%)
Frame = +2
Query: 278 KIKHYADQNTSSGTPQERECSIRKSCNNVSVTSMEDLSHEEI 403
K K Y Q SG + SIRK+C N+ + L H +
Sbjct: 1717 KQKEYVTQLILSGLLNKNTNSIRKTCMNILLYLRRQLGHHAL 1758
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 26.6 bits (56), Expect = 4.6
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +2
Query: 356 NNVSVTSMEDLSHEEIVTSYVLAHVAQFDCRRHHMAFTNGNT 481
+N+SVT+ L + +V++Y L CR AFTNG T
Sbjct: 853 SNISVTNENRLQYIHLVSNYYLNARLSRQCR----AFTNGFT 890
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 26.2 bits (55), Expect = 6.1
Identities = 29/101 (28%), Positives = 46/101 (45%), Gaps = 18/101 (17%)
Frame = -3
Query: 705 VLLVLNRVDSKISATI--------HSLMSWFTVLVPYNSS----SSHWLKNDSCRN---- 574
VL + N + S + A I +SL+SW + +NS S+ +L+ D C+N
Sbjct: 30 VLSLTNSIQSNVGAVISYAVLYFVNSLLSWCMLSSWFNSKLSKLSAGYLQFD-CQNDGKC 88
Query: 573 YPLVMTDPLLRS--KFHLRFALALISCNSLLRVVFPLVNAM 457
Y ++ L + FHL A L CN+ RV + N +
Sbjct: 89 YSVIAVHRLSFTLVMFHLFLAFILSLCNTRSRVAIKIQNGL 129
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 25.8 bits (54), Expect = 8.1
Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 4/51 (7%)
Frame = -3
Query: 738 CLLE-PEVIERVVLLVLN---RVDSKISATIHSLMSWFTVLVPYNSSSSHW 598
C LE PEV+ L ++ R D +SA + W T L+ SS SH+
Sbjct: 604 CFLELPEVLHGKKFLKVDEATREDMLMSAETPDDILWITTLISTGSSMSHF 654
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,370,923
Number of Sequences: 5004
Number of extensions: 65567
Number of successful extensions: 151
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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