Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BmNP13_FL5_D21
         (873 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ...    38   0.002
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit...    31   0.21 
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    26   6.1  
SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr 1||...    26   6.1  
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    26   8.1  

>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 407

 Score = 37.9 bits (84), Expect = 0.002
 Identities = 37/136 (27%), Positives = 60/136 (44%), Gaps = 4/136 (2%)
 Frame = +1

Query: 469 VRIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRAMPNLGIIYGNRDRESDGKMVFDVIQ 648
           +R+++  GF+    KQYR +++ N I SL  +L AM N      N     + +    VI 
Sbjct: 94  MRLLYSPGFSQVVRKQYRVMIFENIISSLCLLLEAMDN-----SNVSLLPENEKYRAVIL 148

Query: 649 RMEDTEPVQ*GASRRDEKALGPTLAFRSALVVQ---RVPLNDSAKYFLDDLDRXGXKDYS 819
           R   ++P +  +    E     TL  +   V      + L D+  Y+ D +DR     Y 
Sbjct: 149 RKHTSQPNEPFSPEIYEAVHALTLDTKLRTVQSCGTNLSLLDNFYYYQDHIDRIFDPQYI 208

Query: 820 PPNK-IS*DXSQTTGI 864
           P ++ I     +TTGI
Sbjct: 209 PSDQDILHCRIKTTGI 224


>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
           Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 354

 Score = 31.1 bits (67), Expect = 0.21
 Identities = 26/119 (21%), Positives = 53/119 (44%), Gaps = 1/119 (0%)
 Frame = +1

Query: 469 VRIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRAMPNLGIIYGNRDRESDGKMVFDVIQ 648
           ++I++++GF+ E+   + PV+  N ++S   +++ +   GI     + +  G    ++I+
Sbjct: 54  IKILNKNGFSQEEIMTFIPVIRRNLLESAKTLVKIIVQKGI-----NLDPLGTHNCEIIE 108

Query: 649 RMEDTEPVQ*GAS-RRDEKALGPTLAFRSALVVQRVPLNDSAKYFLDDLDRXGXKDYSP 822
           +   T      A+  +   +L    + RS        L DSA YF    D    + Y P
Sbjct: 109 KFNPTPGELINANIGQAITSLWSANSVRSCTYGNDSVLIDSAPYFFSRADEICSRHYVP 167


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 26.2 bits (55), Expect = 6.1
 Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 2/45 (4%)
 Frame = -1

Query: 360  PISAVLPMLWPQAA--PTPEPGALSPISQYALYTRPVAPALPEPP 232
            P S + P+  P A   P P P    P+ + ++   PV P    PP
Sbjct: 1174 PSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPP 1218


>SPAC31A2.16 |gef2||RhoGEF Gef2|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1101

 Score = 26.2 bits (55), Expect = 6.1
 Identities = 15/59 (25%), Positives = 30/59 (50%)
 Frame = +1

Query: 400  KQIDR*ELEREWYSGIKRHQAIVVRIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRAM 576
            K+I   ++ERE Y  ++R   + +  + +    NED K+  P++Y      L  I +++
Sbjct: 1006 KEILPGQIEREAYEDLRRKYHLTLARLAQMTRLNEDSKKSIPLLYDRFNHDLKLIKQSV 1064


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 14/39 (35%), Positives = 18/39 (46%), Gaps = 1/39 (2%)
 Frame = -1

Query: 345 LPMLWPQAAPTPEPGALSPISQYAL-YTRPVAPALPEPP 232
           LPM  P A P P    ++P     +    P+ PA P PP
Sbjct: 439 LPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPP 477


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,577,470
Number of Sequences: 5004
Number of extensions: 75022
Number of successful extensions: 226
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)