Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BmNP12_T7_O22
         (860 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual      45   1e-05
SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual      34   0.030
SPBC28F2.03 |ppi1|cyp2|cyclophilin family peptidyl-prolyl cis-tr...    27   2.6  
SPAC637.10c |rpn10|pus1|19S proteasome regulatory subunit Rpn10|...    27   2.6  
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch...    27   3.4  

>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 272

 Score = 45.2 bits (102), Expect = 1e-05
 Identities = 41/171 (23%), Positives = 71/171 (41%), Gaps = 3/171 (1%)
 Frame = -2

Query: 670 FNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEG 491
           F+    + +  +  P+F T +GK+ V IC+         +   NGA+++      +A   
Sbjct: 122 FDTERKHFKKGSDFPIFETSFGKLGVMICWDTAFPEVARIHALNGADLL-----VVATNW 176

Query: 490 GSEYM--WNVEARNAAITNCYFTAAINRVGYEEFPNEFTSADGKPAHKDLGLFYGSSYFC 317
            + Y   W++  +  A  NC    A NRVG +E  +                F+G S   
Sbjct: 177 ENPYSDDWDLVTKARAFENCIPLVAANRVGTDEKLS----------------FFGHSKII 220

Query: 316 GPDGVRCPGLSRTRDGLLIAAVDLNLNRQIRDRRCYYMTQRL-DMYVNSLS 167
           GP G     L   ++G++   VDL+  + +R     +   R+ D+Y   LS
Sbjct: 221 GPTGKVIKALDEEKEGVISYTVDLDDAKPLRKNYYTFFEDRMPDLYKRLLS 271


>SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 276

 Score = 33.9 bits (74), Expect = 0.030
 Identities = 42/150 (28%), Positives = 56/150 (37%), Gaps = 3/150 (2%)
 Frame = -2

Query: 664 ESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQNGAEIVFNPSATIAGEGGS 485
           ESN  + G    P   T  GK+   ICF        +     GA I+  PSA     G +
Sbjct: 130 ESNTTLRGEAILPPCKTPLGKVGSAICFDIRFPEQAIKLRNMGAHIITYPSAFTEKTGAA 189

Query: 484 EYMWNVEARNAAI-TNCYFTAAINRVGYEEFPNEFTSADGKPAHKDLGLFYGSSYFCGPD 308
              W V  R  A+ + CY  A               +  GK  H +    YG S    P 
Sbjct: 190 H--WEVLLRARALDSQCYVIA--------------PAQGGK--HNEKRASYGHSMIVDPW 231

Query: 307 GVRCPGLS--RTRDGLLIAAVDLNLNRQIR 224
           G      S   + +GL+ A +DLNL   +R
Sbjct: 232 GTVIAQYSDISSPNGLIFADLDLNLVDHVR 261


>SPBC28F2.03 |ppi1|cyp2|cyclophilin family peptidyl-prolyl cis-trans
           isomerase Cyp2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 162

 Score = 27.5 bits (58), Expect = 2.6
 Identities = 14/31 (45%), Positives = 16/31 (51%)
 Frame = +2

Query: 566 DVVPSEADVHRDLAVSGREYRMAGVTFHVVV 658
           DVVP  A   R L    + Y  AG TFH V+
Sbjct: 25  DVVPKTAANFRALCTGEKGYGYAGSTFHRVI 55


>SPAC637.10c |rpn10|pus1|19S proteasome regulatory subunit
           Rpn10|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 243

 Score = 27.5 bits (58), Expect = 2.6
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +3

Query: 441 FVIAAFLASTFHMYSLPPSPAIVAD 515
           F+ AA  + + H+ S+PPSP +++D
Sbjct: 156 FIDAANSSDSCHLVSIPPSPQLLSD 180


>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
            Mok12|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2352

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +3

Query: 558  QFKTWCRPKQMFTAILPYLVANTGWPVLPS 647
            +F T    K +FTA+ P+L A T + ++PS
Sbjct: 1521 RFPTRVLCKPVFTAVPPFLFAGTDFALIPS 1550


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,142,982
Number of Sequences: 5004
Number of extensions: 62022
Number of successful extensions: 171
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)