BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP12_FL5_M14
(830 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 34 0.022
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.11
SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 27 4.3
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 25 10.0
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 34.3 bits (75), Expect = 0.022
Identities = 21/67 (31%), Positives = 21/67 (31%), Gaps = 7/67 (10%)
Frame = -1
Query: 470 PPPPPPXXXXXGGXPPXXGXXXFXXPPPPXXXXXFXGG-------XXXXXXPPPXXXXGG 312
PPPPPP G P G PPPP G PPP
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPS 371
Query: 311 XGGXPPP 291
G PPP
Sbjct: 372 TGRQPPP 378
Score = 33.1 bits (72), Expect = 0.050
Identities = 22/69 (31%), Positives = 22/69 (31%), Gaps = 8/69 (11%)
Frame = -3
Query: 471 PPPPPPXXXXXGGXPP-XXGXGXXXXXPPPXKXXXFXGG-------XXXXXXPPPXXXXG 316
PPPPPP G PP G PPP G PPP
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAP 370
Query: 315 GXGGXPPPL 289
G PPPL
Sbjct: 371 STGRQPPPL 379
Score = 29.1 bits (62), Expect = 0.81
Identities = 19/68 (27%), Positives = 20/68 (29%)
Frame = -3
Query: 495 GXGGXXXXPPPPPPXXXXXGGXPPXXGXGXXXXXPPPXKXXXFXGGXXXXXXPPPXXXXG 316
G PPPPPP G P G PPP + G PPP
Sbjct: 330 GSSNSSLPPPPPPPRSNAAGSI-PLPPQGRSAPPPPPPRSAPSTG-----RQPPPLSSSR 383
Query: 315 GXGGXPPP 292
P P
Sbjct: 384 AVSNPPAP 391
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.9 bits (69), Expect = 0.11
Identities = 14/36 (38%), Positives = 14/36 (38%)
Frame = +2
Query: 410 PXPXXGGXPPXXXXXGGGGGGXXXXPPXPXXXXXGG 517
P P GG PP G G G PP P GG
Sbjct: 754 PAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789
Score = 30.7 bits (66), Expect = 0.26
Identities = 18/58 (31%), Positives = 18/58 (31%), Gaps = 5/58 (8%)
Frame = -1
Query: 470 PPPPPPXXXXXGGXP-----PXXGXXXFXXPPPPXXXXXFXGGXXXXXXPPPXXXXGG 312
PPPPPP P P PPPP G PPP GG
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789
Score = 30.3 bits (65), Expect = 0.35
Identities = 16/50 (32%), Positives = 16/50 (32%), Gaps = 3/50 (6%)
Frame = -2
Query: 469 PPPPPXXXXXG---GXXPXPXXXXXFXXPPPPXXXXFXXGGXXXXXPPPP 329
PPPPP P P PPPP G PPPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 30.3 bits (65), Expect = 0.35
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = -3
Query: 516 PPXXXXXGXGGXXXXPPPPPPXXXXXGG 433
PP G G PPPPPP GG
Sbjct: 762 PPPPPPPGVAGAGPPPPPPPPPAVSAGG 789
Score = 29.5 bits (63), Expect = 0.61
Identities = 15/45 (33%), Positives = 15/45 (33%)
Frame = -1
Query: 395 PPPPXXXXXFXGGXXXXXXPPPXXXXGGXGGXPPPFXKKXRGXPP 261
PPPP PPP GG PPP G PP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
Score = 29.1 bits (62), Expect = 0.81
Identities = 14/46 (30%), Positives = 14/46 (30%)
Frame = -1
Query: 467 PPPPPXXXXXGGXPPXXGXXXFXXPPPPXXXXXFXGGXXXXXXPPP 330
PPP P PP G PPPP G P P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/46 (30%), Positives = 14/46 (30%)
Frame = -2
Query: 469 PPPPPXXXXXGGXXPXPXXXXXFXXPPPPXXXXFXXGGXXXXXPPP 332
PPP P P P PPPP GG P P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
Score = 26.6 bits (56), Expect = 4.3
Identities = 20/60 (33%), Positives = 20/60 (33%)
Frame = -3
Query: 471 PPPPPPXXXXXGGXPPXXGXGXXXXXPPPXKXXXFXGGXXXXXXPPPXXXXGGXGGXPPP 292
PPPPPP P PPP GG PPP G G PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPI------PVPPPAP---IMGGPPP---PPPPPGVAGAGPPPPP 779
Score = 26.6 bits (56), Expect = 4.3
Identities = 14/44 (31%), Positives = 14/44 (31%)
Frame = -3
Query: 393 PPPXKXXXFXGGXXXXXXPPPXXXXGGXGGXPPPLFXKXKGXPP 262
PPP PPP GG PPP G PP
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
>SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol
3-phosphatidyltransferase Pis1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 26.6 bits (56), Expect = 4.3
Identities = 15/36 (41%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = -1
Query: 146 SFFXEXPDTKVGLPIDSFRYFSEAIPLKY-TLIAVI 42
SF P LP+ SF Y + +PL Y TL+AV+
Sbjct: 178 SFTPRTPPKLGYLPVPSFIYSTGELPLSYPTLLAVL 213
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 25.4 bits (53), Expect = 10.0
Identities = 19/62 (30%), Positives = 19/62 (30%), Gaps = 3/62 (4%)
Frame = -1
Query: 464 PPPPXXXXXGGX---PPXXGXXXFXXPPPPXXXXXFXGGXXXXXXPPPXXXXGGXGGXPP 294
PPPP G PP PPPP PPP G PP
Sbjct: 286 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEP-----GEHMPPPPMHHEPGEHMPPP 340
Query: 293 PF 288
PF
Sbjct: 341 PF 342
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,964,086
Number of Sequences: 5004
Number of extensions: 34912
Number of successful extensions: 187
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 42
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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