BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP12_FL5_I24
(862 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.01 |did4|SPAC644.03c, vps2|vacuolar sorting protein Did4... 30 0.49
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 29 1.1
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 27 3.4
SPAC56E4.02c |alg13||N-acetylglucosaminyldiphosphodolichol N-ace... 27 4.5
SPAC1F7.09c |||allantoicase |Schizosaccharomyces pombe|chr 1|||M... 26 6.0
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 26 7.9
SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1 |Sc... 26 7.9
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 26 7.9
>SPAC4F8.01 |did4|SPAC644.03c, vps2|vacuolar sorting protein
Did4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 29.9 bits (64), Expect = 0.49
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +1
Query: 223 VRVHRADTGRSSNELDRQTTELERR 297
+R H+ GR+ ELDR+ T+L++R
Sbjct: 18 LRAHQRSLGRAERELDRERTKLDQR 42
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 28.7 bits (61), Expect = 1.1
Identities = 14/48 (29%), Positives = 29/48 (60%)
Frame = +2
Query: 176 IVVNPTLHLRHANIRESESTVRILADLQMNWIDKRLSWNAGGMGLQHL 319
+++NPTLH+ N+ +E +I +++ W+ ++ + GG GL+ L
Sbjct: 992 LLLNPTLHVSEENLPMTEELSKIRIEME-EWL---VNHSTGGSGLKGL 1035
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 27.1 bits (57), Expect = 3.4
Identities = 13/56 (23%), Positives = 24/56 (42%)
Frame = -3
Query: 173 WSTGGDSRSYVARSESIMRDSDVAFSHSAALAIAQVRRNGNQNNISRRHFMTTDLF 6
WS G + +ESI+ + +L ++ R + +N S H + T +F
Sbjct: 242 WSHGLTPEVFWQNTESILTCPEEQLEQKISLLLSSTRNSPTMSNSSLTHLLPTPIF 297
>SPAC56E4.02c |alg13||N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase Alg13
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 26.6 bits (56), Expect = 4.5
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = -3
Query: 146 YVARSESIMRDSDVAFSHSAALAIAQVRRNGNQ 48
Y ES + D+ + SH+ A +I Q R+G +
Sbjct: 63 YAPEIESYIHDASIVISHAGAGSILQTLRSGKR 95
>SPAC1F7.09c |||allantoicase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 342
Score = 26.2 bits (55), Expect = 6.0
Identities = 9/22 (40%), Positives = 15/22 (68%)
Frame = -1
Query: 292 VPTQSFVDPIHLKICQYPHGGL 227
+P Q+F H+++C YP GG+
Sbjct: 153 IPQQNFT---HVRLCMYPDGGI 171
>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
pombe|chr 1||Partial|Manual
Length = 1887
Score = 25.8 bits (54), Expect = 7.9
Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 4/65 (6%)
Frame = -3
Query: 209 HVSNAGSG*LRFWSTGGDSRSYVARSESIMR----DSDVAFSHSAALAIAQVRRNGNQNN 42
H+S G R ++ GG SY A+ + R D+ V ++ SAAL + + R + ++
Sbjct: 433 HLSGMTMGMRRMFTQGG---SYSAQERGLCRLEQKDTVVRYAQSAALYLIFLLRRPSADS 489
Query: 41 ISRRH 27
RRH
Sbjct: 490 GIRRH 494
>SPBC1347.01c |rev1|SPBC215.16c|deoxycytidyl transferase Rev1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 935
Score = 25.8 bits (54), Expect = 7.9
Identities = 10/40 (25%), Positives = 22/40 (55%)
Frame = +1
Query: 406 PVYLITAPSPVIKRLDISTPISMQVRXLXQRHADLHVXVR 525
P++++ P+P+ ++ + I+M R + HAD + R
Sbjct: 732 PLFMVELPTPIRNEVNDNHEIAMNKRLSLKSHADNKIDER 771
>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase
Tlh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1919
Score = 25.8 bits (54), Expect = 7.9
Identities = 20/65 (30%), Positives = 33/65 (50%), Gaps = 4/65 (6%)
Frame = -3
Query: 209 HVSNAGSG*LRFWSTGGDSRSYVARSESIMR----DSDVAFSHSAALAIAQVRRNGNQNN 42
H+S G R ++ GG SY A+ + R D+ V ++ SAAL + + R + ++
Sbjct: 433 HLSGMTMGMRRMFTQGG---SYSAQERGLCRLEQKDTVVRYAQSAALYLIFLLRRPSADS 489
Query: 41 ISRRH 27
RRH
Sbjct: 490 GIRRH 494
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,816,541
Number of Sequences: 5004
Number of extensions: 51162
Number of successful extensions: 148
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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