BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP12_FL5_H13
(851 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.013
SPAC4F8.01 |did4|SPAC644.03c, vps2|vacuolar sorting protein Did4... 30 0.36
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 1.1
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 1.9
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 4.5
SPAC56E4.02c |alg13||N-acetylglucosaminyldiphosphodolichol N-ace... 27 4.5
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 35.1 bits (77), Expect = 0.013
Identities = 25/91 (27%), Positives = 26/91 (28%)
Frame = +1
Query: 577 PPXRQPEFPAXXXPXXXXXVXXPPPXXGAPSGXXXXXGAPXPSXXPXXXRXXXPPPHXAX 756
PP R + P PPP APS P S PPP
Sbjct: 341 PPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQP---PPLSSSRAVSNPPAPPPAIPG 397
Query: 757 XXXXXXXPXGXPSRXHTPPXXXPDPXXPGXP 849
P G SR TPP P P P
Sbjct: 398 RSAPALPPLGNASRTSTPPVPTPPSLPPSAP 428
Score = 26.6 bits (56), Expect = 4.5
Identities = 13/42 (30%), Positives = 15/42 (35%)
Frame = +2
Query: 539 PXPXXPXXXAPXPPPXXNPNSXPAPSPXXXPXXXDPPRXXXP 664
P P AP PPP P++ SP P P P
Sbjct: 241 PPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNP 282
>SPAC4F8.01 |did4|SPAC644.03c, vps2|vacuolar sorting protein
Did4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 30.3 bits (65), Expect = 0.36
Identities = 11/25 (44%), Positives = 19/25 (76%)
Frame = +2
Query: 224 VRVHRANTGRSSNELDRQTTELERR 298
+R H+ + GR+ ELDR+ T+L++R
Sbjct: 18 LRAHQRSLGRAERELDRERTKLDQR 42
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 28.7 bits (61), Expect = 1.1
Identities = 23/102 (22%), Positives = 25/102 (24%)
Frame = +2
Query: 524 PRXXXPXPXXPXXXAPXPPPXXNPNSXPAPSPXXXPXXXDPPRXXXPXAXXXHXXXPXLP 703
P P P P P P P P P P P P P +P
Sbjct: 1136 PSGAPPVPKPSVAAPPVPAPSGAP---PVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVP 1192
Query: 704 XXSPXXXXXXRPXXIXPXXPXXXRXPXGXPXGXTXPPXXXPT 829
S + P P P P PP PT
Sbjct: 1193 PPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAG-LPPVPVPT 1233
Score = 28.3 bits (60), Expect = 1.5
Identities = 25/124 (20%), Positives = 32/124 (25%)
Frame = +2
Query: 458 PXAPKXLSRFSTXXAQRXXAXXPRXXXPXPXXPXXXAPXPPPXXNPNSXPAPSPXXXPXX 637
P PK A P+ P P P P + + P P+P P
Sbjct: 1102 PPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAP-P 1160
Query: 638 XDPPRXXXPXAXXXHXXXPXLPXXSPXXXXXXRPXXIXPXXPXXXRXPXGXPXGXTXPPX 817
P P P +P + P P P P T PP
Sbjct: 1161 VPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPP-VPPPSTAPPV 1219
Query: 818 XXPT 829
P+
Sbjct: 1220 PTPS 1223
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.9 bits (59), Expect = 1.9
Identities = 13/37 (35%), Positives = 13/37 (35%)
Frame = +2
Query: 539 PXPXXPXXXAPXPPPXXNPNSXPAPSPXXXPXXXDPP 649
P P P P P P P PAP P PP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 4.5
Identities = 17/57 (29%), Positives = 18/57 (31%), Gaps = 1/57 (1%)
Frame = +2
Query: 545 PXXPXXXAPXPPPXXNPNSXPAPSPXXXPXXXDPPRXXXP-XAXXXHXXXPXLPXXS 712
P P P P P AP+P P PP P A P LP S
Sbjct: 1683 PAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASS 1739
Score = 26.6 bits (56), Expect = 4.5
Identities = 15/49 (30%), Positives = 19/49 (38%), Gaps = 5/49 (10%)
Frame = +2
Query: 488 STXXAQRXXAXXPRXXXPXPXXPXXXAPXPP-----PXXNPNSXPAPSP 619
ST + A P+ P P P P PP P P++ P P P
Sbjct: 1688 STPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPAC56E4.02c |alg13||N-acetylglucosaminyldiphosphodolichol
N-acetylglucosaminyltransferase Alg13
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 26.6 bits (56), Expect = 4.5
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = -2
Query: 148 YVARSESIMRDSDVAFSHSAALAIAQVRRNGNQ 50
Y ES + D+ + SH+ A +I Q R+G +
Sbjct: 63 YAPEIESYIHDASIVISHAGAGSILQTLRSGKR 95
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,346,601
Number of Sequences: 5004
Number of extensions: 39725
Number of successful extensions: 157
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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