Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP12_FL5_F08
(822 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
DQ026039-1|AAY87898.1| 427|Apis mellifera nicotinic acetylcholi... 36 3e-04
DQ667183-1|ABG75735.1| 463|Apis mellifera GABA-gated ion channe... 36 6e-04
DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 31 0.010
DQ667185-1|ABG75737.1| 447|Apis mellifera glutamate-gated chlor... 31 0.017
DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholi... 31 0.017
AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic ac... 29 0.039
AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic ac... 29 0.052
DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 29 0.069
DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholi... 29 0.069
AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic ac... 28 0.12
AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 27 0.16
EF127805-1|ABL67942.1| 461|Apis mellifera nicotinic acetylcholi... 26 0.48
EF127804-1|ABL67941.1| 461|Apis mellifera nicotinic acetylcholi... 26 0.48
EF127803-1|ABL67940.1| 461|Apis mellifera nicotinic acetylcholi... 26 0.48
EF127802-1|ABL67939.1| 461|Apis mellifera nicotinic acetylcholi... 26 0.48
EF127801-1|ABL67938.1| 461|Apis mellifera nicotinic acetylcholi... 26 0.48
EF127800-1|ABL67937.1| 461|Apis mellifera nicotinic acetylcholi... 26 0.48
DQ026036-1|AAY87895.1| 529|Apis mellifera nicotinic acetylcholi... 26 0.48
DQ026035-1|AAY87894.1| 529|Apis mellifera nicotinic acetylcholi... 26 0.48
DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholi... 25 0.64
DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated... 22 6.0
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 22 6.0
>DQ026039-1|AAY87898.1| 427|Apis mellifera nicotinic acetylcholine
receptor beta2subunit protein.
Length = 427
Score = 36.3 bits (80), Expect = 3e-04
Identities = 21/72 (29%), Positives = 34/72 (47%), Gaps = 5/72 (6%)
Frame = +2
Query: 113 CENATSLSLMIDS---LLATYDRDSPPDSKIV--VNLTLHLRHANIRESESTVRIQADLQ 277
C++ TS S + L YDRD P+ K ++ L ++H N+ E TV L+
Sbjct: 28 CKDITSTSALYRLKLYLFCDYDRDIIPEQKNATKIDFGLSIQHYNVDEYSHTVDFHVMLK 87
Query: 278 MNWIDKRLSWNA 313
+ W L+W +
Sbjct: 88 LMWEQSHLTWKS 99
>DQ667183-1|ABG75735.1| 463|Apis mellifera GABA-gated ion channel
protein.
Length = 463
Score = 35.5 bits (78), Expect = 6e-04
Identities = 33/149 (22%), Positives = 60/149 (40%), Gaps = 6/149 (4%)
Frame = +2
Query: 119 NATSLSLMIDSLLATYDRDSPPD---SKIVVNLTLHLRHAN-IRESESTVRIQADLQMNW 286
N +++S ++D+LL YD PD V + + +R I E + T + + +W
Sbjct: 2 NHSNISELLDNLLRGYDNSVRPDFGGPPATVEVDIMVRSMGPISEVDMTYSMDCYFRQSW 61
Query: 287 IDKRLSWNAGXVGLQHLAGVLGTLVEA*RGTAKRGCXHRRGLRSPRPCI--*XRXRLLEX 460
+D+RL++ G L +L + + + + SP + R+L
Sbjct: 62 VDRRLAFQGGKETLALSISMLARIWKPDTYFYNGKHSYLHTITSPNKFVRLYQDGRVLYS 121
Query: 461 XAWXLPLPIXMXLDTWPXDMQTCXFQXXS 547
+ M L+ +P D Q C Q S
Sbjct: 122 SRLTIKAGCPMNLENFPMDTQRCPLQFGS 150
>DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 31.5 bits (68), Expect = 0.010
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAAAXTXG-DYALRARVSNXGXVSW 455
V S+ +WRPD+VL N A A +A + + G V W
Sbjct: 105 VPSDHIWRPDIVLYNNADGNFEVTLATKATIYHQGLVEW 143
>DQ667185-1|ABG75737.1| 447|Apis mellifera glutamate-gated chloride
channel protein.
Length = 447
Score = 30.7 bits (66), Expect = 0.017
Identities = 39/173 (22%), Positives = 66/173 (38%), Gaps = 10/173 (5%)
Frame = +2
Query: 59 ILFWLPFLLTCAIASAAECENATSLSLMIDSLLATYDRDSPP------DSKIVVNLTLHL 220
+L L FLL + + + ++D++L YD P D +V + L +
Sbjct: 8 LLVLLTFLLHPSRCTQGKVNYREKEKEVLDNILGGYDARIRPSGENATDGPAIVRVNLFV 67
Query: 221 RH-ANIRESESTVRIQADLQMNWIDKRLSWNAGXVGLQHLAGVLGTLV---EA*RGTAKR 388
R A I + + +Q + W+D+RL +N L++L + V + K
Sbjct: 68 RSIATISDIKMEYSVQLTFREQWLDERLRFNDFGGRLKYLTLTDASRVWMPDLFFSNEKE 127
Query: 389 GCXHRRGLRSPRPCI*XRXRLLEXXAWXLPLPIXMXLDTWPXDMQTCXFQXXS 547
G H + + I +L L L M L +P D Q C + S
Sbjct: 128 GHFHNIIMPNVYIRIFPNGSVLYSIRISLTLSCPMNLKLYPLDRQVCSLRMAS 180
>DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholine
receptor alpha3subunit protein.
Length = 566
Score = 30.7 bits (66), Expect = 0.017
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAAAXTXG-DYALRARVSNXGXVSW 455
V S+ +WRPD+VL N A A +A ++ G V W
Sbjct: 101 VPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEW 139
>AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic
acetylcholine receptoralpha-3 protein.
Length = 537
Score = 29.5 bits (63), Expect = 0.039
Identities = 18/48 (37%), Positives = 24/48 (50%), Gaps = 4/48 (8%)
Frame = +3
Query: 324 GCSTWLVSSERLWRPDVVLLNAAAXTXGDYAL----RARVSNXGXVSW 455
G V SE +W PD+VL N A G+Y + +A + G VSW
Sbjct: 104 GVEMLYVPSENIWLPDIVLYNNA---DGNYEVTLMTKATLKYTGDVSW 148
>AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic
acetylcholine receptorApisa2 subunit protein.
Length = 541
Score = 29.1 bits (62), Expect = 0.052
Identities = 19/48 (39%), Positives = 25/48 (52%), Gaps = 2/48 (4%)
Frame = +3
Query: 318 EWGCSTWL-VSSERLWRPDVVLLNAAAXTXGDYAL-RARVSNXGXVSW 455
E+G T L V SE +W PD+VL N A G + +A + G V W
Sbjct: 88 EYGGVTELYVPSEHIWLPDIVLYNNADGEYGVTTMTKAILHYTGKVLW 135
>DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 28.7 bits (61), Expect = 0.069
Identities = 17/42 (40%), Positives = 23/42 (54%), Gaps = 4/42 (9%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAAAXTXGDYAL----RARVSNXGXVSW 455
V S+ +WRPD+VL N A G+Y + +A V G V W
Sbjct: 105 VPSDHIWRPDIVLYNNA---DGNYEVTLMTKATVYYSGLVVW 143
>DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholine
receptor alpha1subunit protein.
Length = 601
Score = 28.7 bits (61), Expect = 0.069
Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 4/48 (8%)
Frame = +3
Query: 324 GCSTWLVSSERLWRPDVVLLNAAAXTXGDYAL----RARVSNXGXVSW 455
G T V SE +W PD+VL N A G+Y + +A + + G V W
Sbjct: 91 GVDTLHVPSEHIWLPDIVLYNNA---DGNYEVTIMTKAILHHTGKVVW 135
>AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic
acetylcholine receptoralpha7-1 protein.
Length = 555
Score = 27.9 bits (59), Expect = 0.12
Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA-AXTXGDYALRARVSNXG 443
+ RLW+PDV++ N+A G Y V N G
Sbjct: 100 IPPHRLWKPDVLMYNSADEGFDGTYPTNVVVKNNG 134
Score = 22.2 bits (45), Expect = 6.0
Identities = 17/83 (20%), Positives = 34/83 (40%), Gaps = 5/83 (6%)
Frame = +2
Query: 92 AIASAAECENATSLSLMIDSLLATYDRDSPP----DSKIVVNLTLHLRHA-NIRESESTV 256
A+A++ + +++ LL TY+ P +V++ L L ++ E +
Sbjct: 11 ALAASGLVNGGSHEKRLLNDLLDTYNVLERPVGNESEPLVLSFGLTLMQIIDVDEKNQLL 70
Query: 257 RIQADLQMNWIDKRLSWNAGXVG 325
L++ W D + WN G
Sbjct: 71 ITNLWLKLEWNDVNMRWNVSDYG 93
>AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic
acetylcholine Apisa7-2 subunit protein.
Length = 461
Score = 27.5 bits (58), Expect = 0.16
Identities = 14/40 (35%), Positives = 18/40 (45%), Gaps = 2/40 (5%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAAAXTXGDYALRAR--VSNXGXVSW 455
V R+WRPD +L N A + VS+ G V W
Sbjct: 82 VPYNRVWRPDTILYNNADPQYSSAVINTNVIVSHTGEVVW 121
>EF127805-1|ABL67942.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 6 protein.
Length = 461
Score = 25.8 bits (54), Expect = 0.48
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRIQADLQMNWIDKRLSWNAGXVG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 25.0 bits (52), Expect = 0.85
Identities = 10/35 (28%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA-AXTXGDYALRARVSNXG 443
++ +LW+PD+++ N+A G Y V++ G
Sbjct: 63 ITPNKLWKPDILMYNSADEGFDGTYQTSVVVTHDG 97
>EF127804-1|ABL67941.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 5 protein.
Length = 461
Score = 25.8 bits (54), Expect = 0.48
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRIQADLQMNWIDKRLSWNAGXVG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 25.0 bits (52), Expect = 0.85
Identities = 10/35 (28%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA-AXTXGDYALRARVSNXG 443
++ +LW+PD+++ N+A G Y V++ G
Sbjct: 63 ITPNKLWKPDILMYNSADEGFDGTYQTSVVVTHDG 97
>EF127803-1|ABL67940.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 4 protein.
Length = 461
Score = 25.8 bits (54), Expect = 0.48
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRIQADLQMNWIDKRLSWNAGXVG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 25.0 bits (52), Expect = 0.85
Identities = 10/35 (28%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA-AXTXGDYALRARVSNXG 443
++ +LW+PD+++ N+A G Y V++ G
Sbjct: 63 ITPNKLWKPDILMYNSADEGFDGTYQTSVVVTHDG 97
>EF127802-1|ABL67939.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 3 protein.
Length = 461
Score = 25.8 bits (54), Expect = 0.48
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRIQADLQMNWIDKRLSWNAGXVG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 25.0 bits (52), Expect = 0.85
Identities = 10/35 (28%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA-AXTXGDYALRARVSNXG 443
++ +LW+PD+++ N+A G Y V++ G
Sbjct: 63 ITPNKLWKPDILMYNSADEGFDGTYQTSVVVTHDG 97
>EF127801-1|ABL67938.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 2 protein.
Length = 461
Score = 25.8 bits (54), Expect = 0.48
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRIQADLQMNWIDKRLSWNAGXVG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 25.0 bits (52), Expect = 0.85
Identities = 10/35 (28%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA-AXTXGDYALRARVSNXG 443
++ +LW+PD+++ N+A G Y V++ G
Sbjct: 63 ITPNKLWKPDILMYNSADEGFDGTYQTSVVVTHDG 97
>EF127800-1|ABL67937.1| 461|Apis mellifera nicotinic acetylcholine
receptor subunitalpha 6 transcript variant 1 protein.
Length = 461
Score = 25.8 bits (54), Expect = 0.48
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRIQADLQMNWIDKRLSWNAGXVG 325
++ E + A L++ W+D L WN G
Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 56
Score = 25.0 bits (52), Expect = 0.85
Identities = 10/35 (28%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA-AXTXGDYALRARVSNXG 443
++ +LW+PD+++ N+A G Y V++ G
Sbjct: 63 ITPNKLWKPDILMYNSADEGFDGTYQTSVVVTHNG 97
>DQ026036-1|AAY87895.1| 529|Apis mellifera nicotinic acetylcholine
receptor alpha6subunit protein.
Length = 529
Score = 25.8 bits (54), Expect = 0.48
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRIQADLQMNWIDKRLSWNAGXVG 325
++ E + A L++ W+D L WN G
Sbjct: 93 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 124
Score = 25.4 bits (53), Expect = 0.64
Identities = 10/35 (28%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA-AXTXGDYALRARVSNXG 443
++ +LW+PD+++ N+A G Y V++ G
Sbjct: 131 ITPNKLWKPDILMYNSADEGFDGTYQTNVVVTHNG 165
>DQ026035-1|AAY87894.1| 529|Apis mellifera nicotinic acetylcholine
receptor alpha6subunit protein.
Length = 529
Score = 25.8 bits (54), Expect = 0.48
Identities = 9/32 (28%), Positives = 15/32 (46%)
Frame = +2
Query: 230 NIRESESTVRIQADLQMNWIDKRLSWNAGXVG 325
++ E + A L++ W+D L WN G
Sbjct: 93 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYG 124
Score = 25.4 bits (53), Expect = 0.64
Identities = 10/35 (28%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 342 VSSERLWRPDVVLLNAA-AXTXGDYALRARVSNXG 443
++ +LW+PD+++ N+A G Y V++ G
Sbjct: 131 ITPNKLWKPDILMYNSADEGFDGTYQTNVVVTHNG 165
>DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholine
receptor beta1subunit protein.
Length = 520
Score = 25.4 bits (53), Expect = 0.64
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 351 ERLWRPDVVLLNAAAXTXGDYALR 422
+++W+PD+VL N A G+Y +R
Sbjct: 106 DKVWKPDIVLFNNA---DGNYEVR 126
Score = 23.8 bits (49), Expect = 2.0
Identities = 12/46 (26%), Positives = 21/46 (45%), Gaps = 1/46 (2%)
Frame = +2
Query: 191 KIVVNLTL-HLRHANIRESESTVRIQADLQMNWIDKRLSWNAGXVG 325
K+ VN L ++ N+ E ++ L+ W D +L W+ G
Sbjct: 51 KVHVNFGLAFVQLINVNEKNQIMKSNVWLRFIWTDYQLQWDEADYG 96
>DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated
ion channel subunit protein.
Length = 510
Score = 22.2 bits (45), Expect = 6.0
Identities = 7/12 (58%), Positives = 8/12 (66%)
Frame = +3
Query: 351 ERLWRPDVVLLN 386
ER+WRPD N
Sbjct: 115 ERIWRPDTYFYN 126
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 22.2 bits (45), Expect = 6.0
Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 2/71 (2%)
Frame = +2
Query: 218 LRHANIRESESTVRIQADLQMNWIDKRLSWNAGXVGLQHLAGVLGTLVEA*--RGTAKRG 391
L+ N+RE + +QA+L D + + G G LV A RGT K+G
Sbjct: 291 LKGINLRELTEAIIVQAELMDLKGDLEGLVEGVIIDCSNHIG-RGKLVTALIQRGTLKKG 349
Query: 392 CXHRRGLRSPR 424
C G+ S +
Sbjct: 350 CLLVSGIASAK 360
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 187,780
Number of Sequences: 438
Number of extensions: 3395
Number of successful extensions: 40
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 40
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 26217432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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