Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP10_FL5_C05
(794 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 244 9e-66
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 235 5e-63
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 129 5e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 110 2e-25
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 30 0.33
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.4
SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 26 7.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 244 bits (598), Expect = 9e-66
Identities = 107/164 (65%), Positives = 133/164 (81%)
Frame = +3
Query: 42 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 221
CLEHGIQP+G M + D F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR
Sbjct: 25 CLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYR 84
Query: 222 QLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXX 401
LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 85 DLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGG 144
Query: 402 XXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSI 533
LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+
Sbjct: 145 TGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSV 188
Score = 100 bits (239), Expect = 3e-22
Identities = 49/77 (63%), Positives = 53/77 (68%)
Frame = +1
Query: 562 SDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRIRRRSECGPHRVPX 741
+DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLR
Sbjct: 198 ADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQT 257
Query: 742 *LGALPPYHFPLVTYAP 792
L P HFPLVTYAP
Sbjct: 258 NLVPYPRIHFPLVTYAP 274
Score = 28.7 bits (61), Expect = 1.0
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +2
Query: 11 QIGNACWELY 40
QIGNACWELY
Sbjct: 15 QIGNACWELY 24
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 235 bits (575), Expect = 5e-63
Identities = 106/169 (62%), Positives = 134/169 (79%), Gaps = 5/169 (2%)
Frame = +3
Query: 42 CLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVR 206
CLEHGI PDG PT+ K +D F TFFSETG GK VPR+++VDLEP V+D+VR
Sbjct: 25 CLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVR 83
Query: 207 TGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 386
TG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 84 TGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFH 143
Query: 387 XXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSI 533
LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+
Sbjct: 144 SFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSV 192
Score = 104 bits (249), Expect = 2e-23
Identities = 51/78 (65%), Positives = 55/78 (70%)
Frame = +1
Query: 559 HSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRIRRRSECGPHRVP 738
+SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASLR +
Sbjct: 201 NSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQ 260
Query: 739 X*LGALPPYHFPLVTYAP 792
L P HFPLVTY+P
Sbjct: 261 TNLVPYPRIHFPLVTYSP 278
Score = 30.7 bits (66), Expect = 0.25
Identities = 15/31 (48%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = +2
Query: 8 VQIGNACWELY-LPGARHPA*WPDAHRQDHR 97
VQIGNACWELY L P +P + + H+
Sbjct: 14 VQIGNACWELYCLEHGIGPDGFPTENSEVHK 44
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 129 bits (311), Expect = 5e-31
Identities = 63/161 (39%), Positives = 91/161 (56%)
Frame = +3
Query: 48 EHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQL 227
EHG+ G T + N +F+E GK+VPRAV VDLEP +D V++G + L
Sbjct: 27 EHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNL 84
Query: 228 FHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 407
F P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H
Sbjct: 85 FRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTG 144
Query: 408 XXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNS 530
LL+ ++ +Y + F++ PAP+ S VVEPYN+
Sbjct: 145 SGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNA 185
Score = 50.0 bits (114), Expect = 4e-07
Identities = 24/79 (30%), Positives = 41/79 (51%)
Frame = +1
Query: 556 KHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRIRRRSECGPHRV 735
++SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T S R ++
Sbjct: 194 ENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKL 253
Query: 736 PX*LGALPPYHFPLVTYAP 792
+ P HF +V +AP
Sbjct: 254 AVNMVPFPRLHFFMVGFAP 272
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 110 bits (265), Expect = 2e-25
Identities = 61/167 (36%), Positives = 95/167 (56%), Gaps = 3/167 (1%)
Frame = +3
Query: 42 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 221
CLEHGI PDG + + T G D + FF ++ +++PRA+ +DLEP VV+ + + TY
Sbjct: 26 CLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYG 83
Query: 222 QLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXX 395
L++PE ++ K A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 84 SLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIA 142
Query: 396 XXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSI 533
L+ERL+ Y KK ++++P Q VS VV+PYNS+
Sbjct: 143 GGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSL 189
Score = 35.9 bits (79), Expect = 0.007
Identities = 21/78 (26%), Positives = 36/78 (46%)
Frame = +1
Query: 559 HSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRIRRRSECGPHRVP 738
++D ++DN A+ I L + PT+ N+L+ ++S+ T +LR +
Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSII 257
Query: 739 X*LGALPPYHFPLVTYAP 792
L P HF L +Y P
Sbjct: 258 ASLIPSPRCHFLLTSYTP 275
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 30.3 bits (65), Expect = 0.33
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = -3
Query: 714 RAPSNSQRSSDRGDNLSNETIQVGVGWALNVEITAADVIDG 592
R+ SN ++ S D N+T VG+G AL I + D +DG
Sbjct: 177 RSNSNDKQKSSDNDEDPNDTT-VGIGAALAASILSVDSVDG 216
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.2 bits (55), Expect = 5.4
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +1
Query: 40 LAWSTASSLMARCPQTRPSGVETILSTLSSARPELAST 153
L ST SSL + ++PS T ST SSA P S+
Sbjct: 170 LTSSTFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 879
Score = 25.8 bits (54), Expect = 7.1
Identities = 13/27 (48%), Positives = 14/27 (51%)
Frame = -3
Query: 750 TKLVWNSVRSTFRAPSNSQRSSDRGDN 670
T+L W S ST A S SSDR N
Sbjct: 760 TQLSWPSTHSTLPATSRELASSDRNIN 786
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 68 WPDAHRQDHRGWRRFFQHFLQR 133
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,326,567
Number of Sequences: 5004
Number of extensions: 67779
Number of successful extensions: 218
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 199
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 387388442
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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