Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP09_FL5_J24
(815 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 341 6e-95
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 337 9e-94
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 175 8e-45
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 114 1e-26
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 32 0.11
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 28 1.8
SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 25 9.7
SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 25 9.7
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 341 bits (839), Expect = 6e-95
Identities = 153/214 (71%), Positives = 181/214 (84%)
Frame = +3
Query: 54 FIQNNLLLGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 233
F ++ GKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 91 FHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTG 150
Query: 234 XXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 413
LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDN
Sbjct: 151 SGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDN 210
Query: 414 EAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIH 593
EA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIH
Sbjct: 211 EACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIH 270
Query: 594 FPLVTYAPVISAEKAYHEQLSVAEITNACFEPAN 695
FPLVTY+P++SA KA+HE SV EITN CFEP N
Sbjct: 271 FPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYN 304
Score = 29.9 bits (64), Expect = 0.45
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +2
Query: 32 TGTYRQLFHPEQLIT 76
TG Y+ LFHPEQ++T
Sbjct: 84 TGPYKDLFHPEQMVT 98
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 337 bits (829), Expect = 9e-94
Identities = 152/214 (71%), Positives = 180/214 (84%)
Frame = +3
Query: 54 FIQNNLLLGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 233
F L+ GKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 87 FHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTG 146
Query: 234 XXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 413
LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDN
Sbjct: 147 SGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDN 206
Query: 414 EAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIH 593
E+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIH
Sbjct: 207 ESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIH 266
Query: 594 FPLVTYAPVISAEKAYHEQLSVAEITNACFEPAN 695
FPLVTYAP++SA KA+HE SV EITN CFEP N
Sbjct: 267 FPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYN 300
Score = 32.3 bits (70), Expect = 0.085
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +2
Query: 32 TGTYRQLFHPEQLIT 76
TG YR LFHPEQLIT
Sbjct: 80 TGPYRDLFHPEQLIT 94
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 175 bits (425), Expect = 8e-45
Identities = 81/214 (37%), Positives = 127/214 (59%)
Frame = +3
Query: 54 FIQNNLLLGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 233
F +N++ G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H
Sbjct: 85 FRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTG 144
Query: 234 XXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 413
LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DN
Sbjct: 145 SGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDN 204
Query: 414 EAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIH 593
EA+ I L I+ P+Y +LN L+ +++ +T S RF G LN DL + N+VP+PR+H
Sbjct: 205 EALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLH 264
Query: 594 FPLVTYAPVISAEKAYHEQLSVAEITNACFEPAN 695
F +V +AP+ + + + +SV E+T F+ N
Sbjct: 265 FFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANN 298
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 114 bits (275), Expect = 1e-26
Identities = 64/193 (33%), Positives = 102/193 (52%), Gaps = 3/193 (1%)
Frame = +3
Query: 48 SCFIQNNLLLGKED--AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXX 221
S + N+L+ K A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 84 SLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIA 142
Query: 222 XXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCA 398
L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D
Sbjct: 143 GGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSV 202
Query: 399 FMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVP 578
++DN A+ I L + PT+ N+L+ ++S+ T +LR+ G +N DL +L+P
Sbjct: 203 VVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIP 262
Query: 579 YPRIHFPLVTYAP 617
PR HF L +Y P
Sbjct: 263 SPRCHFLLTSYTP 275
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 31.9 bits (69), Expect = 0.11
Identities = 17/54 (31%), Positives = 30/54 (55%)
Frame = -1
Query: 578 RHQVSLELGEVHIQSAVESQRSSDRGDNLSNETIQVGVGWALNVEITAADVIDG 417
R + E + H ++ + Q+SSD ++ ++ T VG+G AL I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 27.9 bits (59), Expect = 1.8
Identities = 31/111 (27%), Positives = 47/111 (42%), Gaps = 8/111 (7%)
Frame = -1
Query: 611 VRDQWEVDTGVRHQVSLELGEVHIQSAVESQRSSDRGDNLSNETIQVGVGWALNVEITAA 432
V D VDT V + +A ES R++ D S+ Q + E T A
Sbjct: 454 VADDDAVDTSVTATDDFD-ASASSSNAYESYRNTYTDDMDSSSIYQTSLSNVKTEETTPA 512
Query: 431 DV---IDGFIV-----DHESTVRVLKGCVGGEDRVVGLDDGSGNLRRGVDG 303
+ +DG ++ +HE V+ G E ++ DDGSG +R +DG
Sbjct: 513 EPASKVDGVVLYDFTGEHEG---VITASEGQEFTLLEPDDGSGWVRVKIDG 560
>SPAC6G9.04 |mug79||meiotically upregulated gene
Mug79|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 25.4 bits (53), Expect = 9.7
Identities = 15/58 (25%), Positives = 27/58 (46%)
Frame = -1
Query: 572 QVSLELGEVHIQSAVESQRSSDRGDNLSNETIQVGVGWALNVEITAADVIDGFIVDHE 399
Q ++ VH ++A E R N + QVG +N ++ D+ + F+ D+E
Sbjct: 601 QSQIKSQSVHHENAAEEIRKMRNLRNSRINSQQVGEKVFVNPDVKTMDIQETFLQDYE 658
>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 538
Score = 25.4 bits (53), Expect = 9.7
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = -1
Query: 587 TGVRHQVSLELGEVHIQSAVESQRSSDRGDNLSNETIQVGVG 462
TG+ ++ E I+S+ E ++S+D + SN+ +GVG
Sbjct: 340 TGIIYEAVTEASAPKIKSSAEKKKSADPEADRSNDD-SIGVG 380
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,134,535
Number of Sequences: 5004
Number of extensions: 63878
Number of successful extensions: 200
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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