Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP08_T7_M22
(822 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 246 3e-66
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 241 6e-65
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 100 3e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 1e-07
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 32 0.11
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 29 0.80
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.4
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 26 5.6
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.6
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 9.8
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 246 bits (602), Expect = 3e-66
Identities = 107/133 (80%), Positives = 122/133 (91%)
Frame = -1
Query: 528 PRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 349
PR G+YMA C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG
Sbjct: 311 PRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGS 370
Query: 348 DLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLA 169
+AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLA
Sbjct: 371 GIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLA 430
Query: 168 ALEKDYEEVGMDS 130
ALE+DYEEVG DS
Sbjct: 431 ALERDYEEVGQDS 443
Score = 60.5 bits (140), Expect = 3e-10
Identities = 39/96 (40%), Positives = 44/96 (45%), Gaps = 2/96 (2%)
Frame = -3
Query: 808 RNXXXGXPTXTNLNFXSXQIVSSITXXLXFXGAFKXDLTXVPXXXGALXPVSXSHWSRTR 629
RN PT NLN Q+VSSIT L F G+ DL L P H+
Sbjct: 219 RNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTN---LVPYPRIHFPLVT 275
Query: 628 QSXL--PRRPTMNSFPXAEITNACFEPANQMVKCDP 527
S + + S EITN CFEP NQMVKCDP
Sbjct: 276 YSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP 311
Score = 40.7 bits (91), Expect = 2e-04
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = -2
Query: 662 PRIXFPLVTYAPVXSAEKAYHEQLS 588
PRI FPLVTY+P+ SA KA+HE S
Sbjct: 267 PRIHFPLVTYSPIVSAAKAFHESNS 291
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 241 bits (591), Expect = 6e-65
Identities = 106/133 (79%), Positives = 120/133 (90%)
Frame = -1
Query: 528 PRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGG 349
PR G+YMA C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G
Sbjct: 307 PRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGS 366
Query: 348 DLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLA 169
++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLA
Sbjct: 367 EIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLA 426
Query: 168 ALEKDYEEVGMDS 130
ALE+DYEEVG DS
Sbjct: 427 ALERDYEEVGQDS 439
Score = 57.6 bits (133), Expect = 2e-09
Identities = 37/96 (38%), Positives = 44/96 (45%), Gaps = 2/96 (2%)
Frame = -3
Query: 808 RNXXXGXPTXTNLNFXSXQIVSSITXXLXFXGAFKXDLTXVPXXXGALXPVSXSHWSRTR 629
RN P+ NLN Q+VSSIT L F G+ DL L P H+
Sbjct: 215 RNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTN---LVPYPRIHFPLVT 271
Query: 628 QSXL--PRRPTMNSFPXAEITNACFEPANQMVKCDP 527
+ + + S EITN CFEP NQMVKCDP
Sbjct: 272 YAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP 307
Score = 41.9 bits (94), Expect = 1e-04
Identities = 18/25 (72%), Positives = 20/25 (80%)
Frame = -2
Query: 662 PRIXFPLVTYAPVXSAEKAYHEQLS 588
PRI FPLVTYAP+ SA KA+HE S
Sbjct: 263 PRIHFPLVTYAPIVSAAKAFHESNS 287
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 100 bits (239), Expect = 3e-22
Identities = 51/137 (37%), Positives = 79/137 (57%), Gaps = 3/137 (2%)
Frame = -1
Query: 534 ATPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVP 355
A PRHG+Y+ L+RG V K+V+ I +++TK + FV+W P + PP
Sbjct: 303 ADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK--- 359
Query: 354 GGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR-- 181
DL + + + N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA
Sbjct: 360 --DL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESN 414
Query: 180 -EDLAALEKDYEEVGMD 133
DL + + Y+E G+D
Sbjct: 415 MNDLVSEYQQYQEAGID 431
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 52.0 bits (119), Expect = 1e-07
Identities = 31/119 (26%), Positives = 61/119 (51%), Gaps = 3/119 (2%)
Frame = -1
Query: 495 LYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCM 316
+ +G+ P DV+ ++ I+ +R F+ W P +V ++ + P + ++ + M
Sbjct: 323 IIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL-----M 377
Query: 315 LSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE---GEFSEAREDLAALEKDYE 148
L+N T+IA + R ++D + + AF+ Y E + E EF +R+ +A L +YE
Sbjct: 378 LANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYE 436
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 31.9 bits (69), Expect = 0.11
Identities = 20/66 (30%), Positives = 26/66 (39%)
Frame = -2
Query: 551 QPDGEMRPPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 372
QP P + S VV P + RP++P P LS V PV+ V +
Sbjct: 531 QPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVP 590
Query: 371 HPPWCP 354
PP P
Sbjct: 591 QPPVAP 596
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 29.1 bits (62), Expect = 0.80
Identities = 12/29 (41%), Positives = 13/29 (44%)
Frame = +3
Query: 471 WVRRHHGTAYSKPCTCHDGGSHFTIWLAG 557
W R H Y+KPC DGG W G
Sbjct: 16 WRRDHPFGFYAKPCKSSDGGLDLMNWKVG 44
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 28.3 bits (60), Expect = 1.4
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -2
Query: 284 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 111
G+ TS TS T S S++ S+P P W P+ S+ TP+ V
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207
Query: 110 EPKSTK 93
EP+ TK
Sbjct: 208 EPRFTK 213
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 26.2 bits (55), Expect = 5.6
Identities = 12/44 (27%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = -2
Query: 635 YAPVXSAEKAYHEQLSXXRDHKRMLRARQPDGE-MRPPVMASTW 507
Y+P+ + AY+ +S +HK ++ GE + P +A +W
Sbjct: 214 YSPLANQNDAYNSDISKLIEHKTLVDIANARGEGITPANIAISW 257
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.6
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -2
Query: 452 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 366
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 9.8
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = -2
Query: 455 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 360
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,816,162
Number of Sequences: 5004
Number of extensions: 56988
Number of successful extensions: 207
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 402440190
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -