Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP08_T7_M20
(782 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 227 1e-60
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 84 2e-17
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 64 2e-11
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 36 0.005
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 32 0.081
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 29 0.75
SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pom... 27 2.3
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 27 2.3
SPBP8B7.07c |set6||histone lysine methyltransferase Set6 |Schizo... 27 3.0
SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces... 27 4.0
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 227 bits (555), Expect = 1e-60
Identities = 108/186 (58%), Positives = 130/186 (69%), Gaps = 1/186 (0%)
Frame = -1
Query: 671 DRVLASPEGQ-LVYELXXXXXXXXXXXKRMTYIEAIEYLRANNITKEDGSFYEFGEDIPE 495
DR+L P L+ +L R++Y +AI+YL +NI +G ++FG+DI E
Sbjct: 382 DRLLDDPIATPLIKQLNPDFVKPSRPFMRLSYEDAIKYLNEHNILTPEGEQHKFGDDIAE 441
Query: 494 MPERKMTDAIGVPILLCKFPAEIKSFYMSRCVDDRRLTESVDVLMPGVGEIVGGSMRIWD 315
ERKMTD I PI L FP EIKSFYM R VD LTESVD LMP VGEIVGGSMRI D
Sbjct: 442 AAERKMTDQINRPIFLTYFPLEIKSFYMKRVVDRPELTESVDCLMPNVGEIVGGSMRISD 501
Query: 314 HEELMEGYKREGIDPSPYYWYTDQRKFGSVPHGGYGLGLERFLCWLLDRYHIRDVCLYPR 135
+EL+ YKREGIDP+PYYW+T+QRK+G+ HGG GLGLERFL WL DRY +R+ CL+PR
Sbjct: 502 IQELLAAYKREGIDPAPYYWFTEQRKYGTTEHGGCGLGLERFLAWLCDRYTVRECCLFPR 561
Query: 134 FLERCT 117
F ERCT
Sbjct: 562 FTERCT 567
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 83.8 bits (198), Expect = 2e-17
Identities = 53/153 (34%), Positives = 84/153 (54%), Gaps = 2/153 (1%)
Frame = -1
Query: 587 MTYIEAIEYLRANNITKEDGSFYEFGEDIPEMPERKMTDAIG-VPILLCKFPAEIKSFYM 411
MTY EAIE L A T + ++G D+ E+ + + + P+ + +P +IK FYM
Sbjct: 293 MTYSEAIEELSAVKKTWKYPP--KWGNDLSSEHEKYLCEILHKTPVFVTDYPQKIKPFYM 350
Query: 410 SRCVDDRRLTESVDVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTDQRKFG 231
D +VD+L+P VGE+ GGS+R DH ++ YK P WY D K+
Sbjct: 351 KSSGPDT--VAAVDLLVPQVGELAGGSLRK-DH---LDEYKTY---PPELQWYLDLMKYS 401
Query: 230 SVPHGGYGLGLERFLCWLL-DRYHIRDVCLYPR 135
+ PHGG+GLG+ER + +L + ++++ +PR
Sbjct: 402 NAPHGGFGLGIERLIAFLEGENTNVKETIPFPR 434
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 64.5 bits (150), Expect = 2e-11
Identities = 38/104 (36%), Positives = 54/104 (51%), Gaps = 1/104 (0%)
Frame = -1
Query: 443 KFPAEIKSFYMSRCVDDRRLTESVDVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSP 264
K+P+ ++ FY +D R + S D M G EI+ G+ RI D E L+E K G+ P
Sbjct: 471 KYPSSVRPFYTMPDPEDPRYSNSYDFFMKGQ-EIMSGAQRIHDPELLVERMKALGVSPDV 529
Query: 263 -YYWYTDQRKFGSVPHGGYGLGLERFLCWLLDRYHIRDVCLYPR 135
Y D G PH G G+GLER + + L+ +IR +PR
Sbjct: 530 GLQQYIDAFAIGCPPHAGGGIGLERVVMFYLNLPNIRLASSFPR 573
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 36.3 bits (80), Expect = 0.005
Identities = 14/34 (41%), Positives = 24/34 (70%)
Frame = -1
Query: 239 KFGSVPHGGYGLGLERFLCWLLDRYHIRDVCLYP 138
++G P GG+G+G++R + +L D IR+V L+P
Sbjct: 542 EYGLPPTGGWGMGVDRLVMFLTDSNTIREVLLFP 575
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 32.3 bits (70), Expect = 0.081
Identities = 24/85 (28%), Positives = 40/85 (47%), Gaps = 6/85 (7%)
Frame = -1
Query: 371 DVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTDQRKF------GSVPHGGY 210
D+++ G+ E+ GGS+RI H ++ + + + P Y G PHGG
Sbjct: 495 DIVVNGI-ELGGGSIRI--HNPDIQRFVLKDVLKLPENRYATFEHLIRVLSSGCPPHGGI 551
Query: 209 GLGLERFLCWLLDRYHIRDVCLYPR 135
LG +R L + IR+V +P+
Sbjct: 552 ALGFDRLAALLTNAPGIREVIAFPK 576
>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2176
Score = 29.1 bits (62), Expect = 0.75
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +2
Query: 440 TCTAVSARQWRPSSSALAFPGCPLQTRRRI 529
TC + R W S FP CP + RR+
Sbjct: 1152 TCKMIEKRLWPTMSPLRQFPNCPSEVIRRV 1181
>SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 781
Score = 27.5 bits (58), Expect = 2.3
Identities = 9/22 (40%), Positives = 12/22 (54%)
Frame = -1
Query: 236 FGSVPHGGYGLGLERFLCWLLD 171
FG+ P Y + F+CWL D
Sbjct: 201 FGTSPFNAYQQNTDNFVCWLFD 222
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 27.5 bits (58), Expect = 2.3
Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = -1
Query: 380 ESVDVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTD--QRKFGSV 225
++V + G+ EI+ + D E+L + Y +EG+ P WY D +R F ++
Sbjct: 989 KTVAAKLSGMKEILFPKSNLADWEQLPD-YVKEGLTGVPVAWYDDVFKRVFSNI 1041
>SPBP8B7.07c |set6||histone lysine methyltransferase Set6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 483
Score = 27.1 bits (57), Expect = 3.0
Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 3/26 (11%)
Frame = -1
Query: 236 FGSVPH---GGYGLGLERFLCWLLDR 168
FG + H G Y + L+ F+ WLLDR
Sbjct: 396 FGGLHHCILGRYSISLDDFVLWLLDR 421
>SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 249
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +3
Query: 132 EARVETHVPDVVPVQEPAEEALQTQPVAAVRHRAELA 242
+ R + +PD+V V +P EE T+P + E A
Sbjct: 196 KTRQKKSLPDIVVVLDPKEEEPITKPYTVINQPVEAA 232
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,724,391
Number of Sequences: 5004
Number of extensions: 51611
Number of successful extensions: 140
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 379359666
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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