Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP08_T7_E12
(831 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 203 3e-53
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 96 6e-21
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 93 6e-20
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 92 1e-19
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 74 2e-14
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 60 4e-10
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 49 7e-07
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 47 3e-06
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 44 2e-05
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 28 1.9
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.9
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 7.5
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 7.5
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 26 7.5
SPAC1B3.17 |clr2||chromatin silencing protein Clr2|Schizosacchar... 25 10.0
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 203 bits (495), Expect = 3e-53
Identities = 92/104 (88%), Positives = 97/104 (93%)
Frame = -2
Query: 638 AAXHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIA 459
A HE TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADRMQKEI ALAPS+MK+KI+A
Sbjct: 272 AGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVA 331
Query: 458 PPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 327
PPERKYSVWIGGSILASLSTFQQMWISKQEYDESGP IV+RKCF
Sbjct: 332 PPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375
Score = 60.9 bits (141), Expect = 2e-10
Identities = 27/34 (79%), Positives = 28/34 (82%)
Frame = -1
Query: 735 YEFPDGQVIXIGNERFRCPEALFQPSFLGMEACG 634
YE PDGQVI IGNERFR PEALFQPS LG+E G
Sbjct: 240 YELPDGQVITIGNERFRAPEALFQPSALGLENAG 273
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 95.9 bits (228), Expect = 6e-21
Identities = 45/119 (37%), Positives = 75/119 (63%)
Frame = -2
Query: 683 AQRLXSNPRSWVWKLAAXHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKE 504
A + NP + + H + SI+ D+D+R LY+N VLSGG+T+ G +R E
Sbjct: 262 APEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISE 321
Query: 503 ITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 327
+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ +EY ++ +++ R+ F
Sbjct: 322 LRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379
Score = 42.3 bits (95), Expect = 8e-05
Identities = 17/34 (50%), Positives = 20/34 (58%)
Frame = -1
Query: 735 YEFPDGQVIXIGNERFRCPEALFQPSFLGMEACG 634
Y+ PD Q I +G E F PE LF P +G EA G
Sbjct: 245 YQLPDNQTITLGTECFSAPEVLFNPEMMGSEASG 278
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 92.7 bits (220), Expect = 6e-20
Identities = 45/101 (44%), Positives = 68/101 (67%), Gaps = 6/101 (5%)
Frame = -2
Query: 614 NSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERK 444
N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A ER
Sbjct: 333 NCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERS 391
Query: 443 YSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 330
Y+ W+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C
Sbjct: 392 YASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432
Score = 33.1 bits (72), Expect = 0.050
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = -1
Query: 735 YEFPDGQVIXIGNERFRCPEALFQP 661
+EFPDG + G ER++ PE LF P
Sbjct: 264 FEFPDGSRMMFGAERYQIPEHLFVP 288
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 91.9 bits (218), Expect = 1e-19
Identities = 43/94 (45%), Positives = 60/94 (63%), Gaps = 2/94 (2%)
Frame = -2
Query: 629 HETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKII-AP 456
HE Y SI+ CD ++R L N V++GGT++ PG+++R+Q E+ LA S + +
Sbjct: 325 HELVYQSILACDSELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETA 384
Query: 455 PERKYSVWIGGSILASLSTFQQMWISKQEYDESG 354
+VW GGSILASL FQ +W+SKQEYDE G
Sbjct: 385 SATSNAVWFGGSILASLDNFQHLWVSKQEYDEVG 418
Score = 37.5 bits (83), Expect = 0.002
Identities = 16/27 (59%), Positives = 17/27 (62%)
Frame = -1
Query: 735 YEFPDGQVIXIGNERFRCPEALFQPSF 655
+EFPDG G ERFR E LF PSF
Sbjct: 281 FEFPDGVTHKFGQERFRISEILFNPSF 307
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 74.1 bits (174), Expect = 2e-14
Identities = 42/103 (40%), Positives = 64/103 (62%), Gaps = 12/103 (11%)
Frame = -2
Query: 626 ETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITAL--------APSTM-- 477
E +++I DVDIRK LY VLSGG++MY G+ R++KEI L P+ +
Sbjct: 276 EFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPN 335
Query: 476 -KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKQEYDESG 354
K+KI P R+++V+IGG++LA ++ MW+SK E++E G
Sbjct: 336 FKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378
Score = 48.8 bits (111), Expect = 9e-07
Identities = 19/34 (55%), Positives = 24/34 (70%)
Frame = -1
Query: 735 YEFPDGQVIXIGNERFRCPEALFQPSFLGMEACG 634
Y PDG+VI +G+ER+ CPE LFQP +G E G
Sbjct: 240 YTLPDGRVIKVGSERYECPECLFQPHLVGSEQPG 273
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 60.1 bits (139), Expect = 4e-10
Identities = 32/111 (28%), Positives = 55/111 (49%), Gaps = 14/111 (12%)
Frame = -2
Query: 626 ETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST----------- 480
E N + +D+RK LY N VLSGG+T++ +R+Q+++ +
Sbjct: 309 ELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAK 368
Query: 479 ---MKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 336
+ + +I+ ++ +VW GGS+LA F +K +Y+E G SI R
Sbjct: 369 SGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 49.2 bits (112), Expect = 7e-07
Identities = 29/102 (28%), Positives = 47/102 (46%)
Frame = -2
Query: 638 AAXHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIA 459
A E S+ +I+ L N V GG +PG R+ E+ +LAP+ ++K+
Sbjct: 298 AGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFE 357
Query: 458 PPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRK 333
P + W S + L + I++ EY E G +I+ RK
Sbjct: 358 PSDPICFPWKKASHM-PLEHWNANKITRSEYSEHGANIMTRK 398
Score = 33.5 bits (73), Expect = 0.038
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = -1
Query: 717 QVIXIGNERFRCPEALFQPSFLGMEACGXP 628
QV+ + NERF PE LF PS + + G P
Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIP 301
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 47.2 bits (107), Expect = 3e-06
Identities = 20/89 (22%), Positives = 45/89 (50%)
Frame = -2
Query: 608 IMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWI 429
+ + ++ ++ L +N +++GG PG+ R+++E+T++ P I + W
Sbjct: 623 LQRHSLEEQQKLVSNVLITGGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWK 682
Query: 428 GGSILASLSTFQQMWISKQEYDESGPSIV 342
G S + F+ ++++EY E GP +
Sbjct: 683 GASEWSVTEKFKAAKVTREEYLEKGPEYI 711
Score = 26.6 bits (56), Expect = 4.3
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -1
Query: 696 ERFRCPEALFQPSFLGMEACG 634
E+ R PE +F PS +G++ G
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAG 614
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 44.4 bits (100), Expect = 2e-05
Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%)
Frame = -2
Query: 581 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 411
K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586
Query: 410 SLSTFQQMWISKQEYDESGPSIVHRK 333
+ ++WI ++ G ++ K
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612
>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 27.9 bits (59), Expect = 1.9
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = +1
Query: 574 KSLRMSTSHFMMELYVVSWXAASFHTQERGLEXSLWATESFVSD 705
++L ST H + EL V + H Q L L T SFV+D
Sbjct: 151 ETLLSSTQHQLTELKDVMHSMSDSHEQNNALHPPLSRTTSFVAD 194
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 27.9 bits (59), Expect = 1.9
Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Frame = -2
Query: 434 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 333
++GGSI+A S + + +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.8 bits (54), Expect = 7.5
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = -3
Query: 442 TPYGSVDRSSPPSLPSNRCGSRNRSTTSLAPP 347
TP + PPS+PS+R R S ++ APP
Sbjct: 223 TPTSTSAPPIPPSIPSSRPPERVPSLSAPAPP 254
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 25.8 bits (54), Expect = 7.5
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -2
Query: 509 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 399
KEI++ A S + + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 25.8 bits (54), Expect = 7.5
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = -1
Query: 345 CTQEVLLNAPRVAVSSSPRPAAQFRLVISVNLIVRKI 235
C +LL+AP + V+ +P +F+L ++ + R I
Sbjct: 1973 CQSPILLDAPTIFVTMNPGYLGRFKLPSNLKKLFRPI 2009
>SPAC1B3.17 |clr2||chromatin silencing protein
Clr2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 537
Score = 25.4 bits (53), Expect = 10.0
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = -1
Query: 552 RWYHHVPWNRRPYAKGNHSSRPIDNED*DHCSSREEVLRMDRWIDPRLPLYLPTDVDLET 373
RWY PW + KG+ + +E + SSR + + W+D YL +++D+ +
Sbjct: 483 RWYE--PW----FVKGDLN---YTSEVKERTSSRLSAVGSENWVDDDFYEYLLSEIDMVS 533
Query: 372 GV 367
V
Sbjct: 534 AV 535
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,888,183
Number of Sequences: 5004
Number of extensions: 57186
Number of successful extensions: 164
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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