Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP08_FL5_O09
(867 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 162 5e-41
SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 162 5e-41
SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosacc... 57 3e-09
SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 35 0.013
SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc... 31 0.21
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 29 0.86
SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 29 1.1
SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 27 2.6
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 27 2.6
SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosac... 27 3.5
SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pom... 27 4.6
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 27 4.6
SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces... 26 6.0
SPAC1002.05c |jmj2||histone demethylase Jmj2 |Schizosaccharomyce... 26 6.0
SPAC824.09c |||GTPase activating protein |Schizosaccharomyces po... 26 8.0
SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 26 8.0
>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 446
Score = 162 bits (394), Expect = 5e-41
Identities = 79/247 (31%), Positives = 139/247 (56%), Gaps = 10/247 (4%)
Frame = +1
Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHIKR 327
MI GLF++N KG+ LI + +R D+ ++ + FRV ++ R P+ +I +++ + K
Sbjct: 1 MISGLFIFNLKGDTLICKTFRHDLKKSVTEIFRVAIL-TNTDYRHPIVSIGSSTYIYTKH 59
Query: 328 ANVWLAAVTKQNVNAAMVFEFVLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGY 507
++++ A+TK N N +V EF+ +I + YFGK++E +K+N I+ELLDE++D+G
Sbjct: 60 EDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKDNVSFIFELLDEMIDYGI 119
Query: 508 PQNSDTGVLKTFITQQGIK------SASKEEQAQI----TSQVTGQIGWRREGIKYRRNE 657
Q ++ L ++ +K S + +Q+ +S++ G + WRR GIKYR+N
Sbjct: 120 IQTTEPDALARSVSITAVKKKGNALSLKRSHSSQLAHTTSSEIPGSVPWRRAGIKYRKNS 179
Query: 658 LFXXXXXXXXXXXXPQGQVLSAHVAGKVVMKSYLSGMPXCKFGINDKSXWNPXKXKWRXS 837
++ G VL + V+G V M++ LSGMP C+FG+NDK + + + +
Sbjct: 180 IYIDIVERMNLLISSTGNVLRSDVSGVVKMRAMLSGMPECQFGLNDKLDFKLKQSESKSK 239
Query: 838 GNXDXDP 858
N +P
Sbjct: 240 SNNSRNP 246
>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 162 bits (394), Expect = 5e-41
Identities = 85/219 (38%), Positives = 129/219 (58%), Gaps = 2/219 (0%)
Frame = +1
Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRV--NVIHARQQVRSPVTNIARTSFFHI 321
M +FV N KG+V+ISR YR DI + V+ F + + Q +P ++ +I
Sbjct: 1 MASAIFVLNLKGKVIISRDYRADIPMSVVEKFLPLKSEVEEEQGFSTPCLTHEGINYIYI 60
Query: 322 KRANVWLAAVTKQNVNAAMVFEFVLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDF 501
+V+L A++K N +A + F+ K+ DV YF ++ EE+I++NFVL+YELLDEI+DF
Sbjct: 61 HHNDVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEIMDF 120
Query: 502 GYPQNSDTGVLKTFITQQGIKSASKEEQAQITSQVTGQIGWRREGIKYRRNELFXXXXXX 681
G+PQ ++T +L+ +ITQ S + ++ A +T I WR EGI YR+NE+F
Sbjct: 121 GFPQTTETKILQEYITQ---TSNTVKKHAPPPIAMTNAISWRSEGIHYRKNEVFLDVIES 177
Query: 682 XXXXXXPQGQVLSAHVAGKVVMKSYLSGMPXCKFGINDK 798
G V+ + + GKV +K YLSGMP + G+NDK
Sbjct: 178 VNLIAAADGTVIQSEILGKVRLKCYLSGMPELRLGLNDK 216
>SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 425
Score = 57.2 bits (132), Expect = 3e-09
Identities = 34/150 (22%), Positives = 63/150 (42%), Gaps = 3/150 (2%)
Frame = +1
Query: 331 NVWLAAVTKQNVNAAMVFEFVLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYP 510
+V L T + + + + +I+DV++++FG + ++ N +I +LL E++D+GY
Sbjct: 66 DVRLCIPTTCDTEPLYIHDIMRRIVDVVKTFFGGFNASKVEKNVCVIVQLLAEMIDYGYA 125
Query: 511 QNSDTGVLKTFITQQGIKS---ASKEEQAQITSQVTGQIGWRREGIKYRRNELFXXXXXX 681
+ L+ + + A Q + + WR KY NE F
Sbjct: 126 TCMEPNALQDIVPLPSFMNKFMAVTGLQTNTPTLARDTVPWRTAKAKYATNEFFIHVLER 185
Query: 682 XXXXXXPQGQVLSAHVAGKVVMKSYLSGMP 771
P G++ V + K +SGMP
Sbjct: 186 VSAVYQPNGKLAFGTVKSDMECKCQISGMP 215
>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 143
Score = 35.1 bits (77), Expect = 0.013
Identities = 25/119 (21%), Positives = 54/119 (45%), Gaps = 3/119 (2%)
Frame = +1
Query: 148 MIGGLFVYNHKGEVLISRVYR--DDIGRNAVDAFRVNVIHAR-QQVRSPVTNIARTSFFH 318
MI + + N G+ +S+ Y DD + + A +I R Q+ ++ + +
Sbjct: 1 MIQFILIQNRHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVY 60
Query: 319 IKRANVWLAAVTKQNVNAAMVFEFVLKIIDVMQSYFGKISEENIKNNFVLIYELLDEIL 495
+ A ++ N + E + ++++ S+FG + E ++ NF + +LDEI+
Sbjct: 61 RRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEII 119
>SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 240
Score = 31.1 bits (67), Expect = 0.21
Identities = 27/140 (19%), Positives = 57/140 (40%), Gaps = 2/140 (1%)
Frame = +1
Query: 166 VYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTS--FFHIKRANVW 339
+ N G+ +ISR +R+ + R V++ ++ A +S T + + F + ++
Sbjct: 8 IVNRGGKAIISRQFRE-MSRVRVESL-LSSFPALVSEKSQNTTVESDNVRFVYQPLDELY 65
Query: 340 LAAVTKQNVNAAMVFEFVLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQNS 519
+ +T N + + + V+ S + E I I+ DE GY N
Sbjct: 66 IVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLGYRDNV 125
Query: 520 DTGVLKTFITQQGIKSASKE 579
+KT++ + + +E
Sbjct: 126 SLTQIKTYLEMESHEEKIQE 145
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 29.1 bits (62), Expect = 0.86
Identities = 18/51 (35%), Positives = 26/51 (50%)
Frame = -1
Query: 507 VTKVQDLIKQLIDQNKVILDVLFRNLAKIRLHHINDLQHELKYHGRVHILL 355
+ +++ L K L+ + K D F LAKI N HEL + G +H LL
Sbjct: 858 LVQLKQLSKHLLSETKQ--DESFSELAKIFQEGSNITSHELLHSGLIHNLL 906
>SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 483
Score = 28.7 bits (61), Expect = 1.1
Identities = 12/46 (26%), Positives = 29/46 (63%)
Frame = +1
Query: 316 HIKRANVWLAAVTKQNVNAAMVFEFVLKIIDVMQSYFGKISEENIK 453
+I+ N + + +++ + A++ E +++DV QS+ G + EEN++
Sbjct: 414 NIEITNPIIVSPVERSRSQALLNEIQTRVLDVRQSHGGVVLEENVR 459
>SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 879
Score = 27.5 bits (58), Expect = 2.6
Identities = 15/49 (30%), Positives = 25/49 (51%)
Frame = -3
Query: 517 NSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTRTQIPWP 371
+S + RS AT +S++S S + S T S+ ++ TQ+ WP
Sbjct: 719 DSGSRRVTRSQNATSQSQESGS--EIGSSIAGTAGSYNVGTSNTQLSWP 765
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 27.5 bits (58), Expect = 2.6
Identities = 14/50 (28%), Positives = 20/50 (40%)
Frame = -1
Query: 249 HSECVHSITTNIISVDSRDQHLAFMVINEQAPDHCG*GLTLFQNWFQCNC 100
H +C + T IS S + IN + P H + NW C+C
Sbjct: 442 HKKCYPKVVTKCISKSSDSASSEYEKINHRIPHHFESHTNIGANWC-CHC 490
>SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 165
Score = 27.1 bits (57), Expect = 3.5
Identities = 21/127 (16%), Positives = 55/127 (43%), Gaps = 8/127 (6%)
Frame = +1
Query: 148 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPV------TNI--AR 303
MI +F++N+KG+ +++ Y ++ + I+A R P +N+ +
Sbjct: 1 MIYAVFIFNNKGKPRLTKFYTPI--DESIQQKLIGDIYAAVSTRPPTACNFLESNLIAGK 58
Query: 304 TSFFHIKRANVWLAAVTKQNVNAAMVFEFVLKIIDVMQSYFGKISEENIKNNFVLIYELL 483
+ + A ++ V + + + + + ++ + F + E ++ F I+ +L
Sbjct: 59 NRIIYRQYATLYFVFVVDEGESELGILDLIQVFVEALDRCFNNVCELDLVFKFQEIHAIL 118
Query: 484 DEILDFG 504
E++ G
Sbjct: 119 AEVVSGG 125
>SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1188
Score = 26.6 bits (56), Expect = 4.6
Identities = 23/102 (22%), Positives = 48/102 (47%), Gaps = 11/102 (10%)
Frame = +1
Query: 238 AFRVNVIHARQQVRSPVTNIARTSFFHIKRANVWLAAVTKQNVNAAMVFEFVLKIIDVMQ 417
+ RVN+ + +S +N + T+ +H++ + + +A+VFEF K +
Sbjct: 839 SIRVNIAGVKITAKSSTSNSSSTAEYHVRSRHAVIP--VNNRYRSAVVFEF-RKQLQRKH 895
Query: 418 SYFGKI----SEENIKNNF-VLIY------ELLDEILDFGYP 510
+ F + E+N++ N V I+ +L ++DF +P
Sbjct: 896 NVFAMVWLVDLEDNVEQNIRVPIFTSSKPAHVLQNMIDFDHP 937
>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1364
Score = 26.6 bits (56), Expect = 4.6
Identities = 15/47 (31%), Positives = 22/47 (46%)
Frame = +1
Query: 415 QSYFGKISEENIKNNFVLIYELLDEILDFGYPQNSDTGVLKTFITQQ 555
+ + KI EE +K N +L YE D +S ++KTF Q
Sbjct: 952 KDFVTKIEEETLKENPLLFYEYRKASSDMRVLMDSQFLMMKTFARLQ 998
>SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 180
Score = 26.2 bits (55), Expect = 6.0
Identities = 11/37 (29%), Positives = 23/37 (62%)
Frame = +1
Query: 409 VMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQNS 519
V++++ G+I+++ ++ V+IY + D L FG S
Sbjct: 113 VLKAHVGRITDDTPQHQGVVIYSMNDTPLGFGVTARS 149
>SPAC1002.05c |jmj2||histone demethylase Jmj2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 715
Score = 26.2 bits (55), Expect = 6.0
Identities = 8/24 (33%), Positives = 13/24 (54%)
Frame = -1
Query: 354 CDCCQPNICTLDMEERCACNVGNR 283
C C +C L +E C C++ N+
Sbjct: 675 CSCKSITVCPLHIEYLCKCDLSNK 698
>SPAC824.09c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 320
Score = 25.8 bits (54), Expect = 8.0
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +2
Query: 272 RCDHLLPTLHAHLSSISSVQMFGWQQSQSKM*TR 373
RC + +L H+S + SV + W Q++ TR
Sbjct: 51 RCSGVHRSLGVHVSRVKSVDLDSWTDEQTENMTR 84
>SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 878
Score = 25.8 bits (54), Expect = 8.0
Identities = 16/58 (27%), Positives = 28/58 (48%)
Frame = +1
Query: 64 YVQFLKGCNR*IAITLKPILKECEASPTMIGGLFVYNHKGEVLISRVYRDDIGRNAVD 237
+V ++ G R I + L P+L PT+ +NH G++ + R + G N+ D
Sbjct: 737 HVSYVTGVIRSIDLQL-PVLLSKFMKPTIFDSYDFFNHWGQMGVEREAQLTFGLNSKD 793
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,342,083
Number of Sequences: 5004
Number of extensions: 66087
Number of successful extensions: 192
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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