Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP08_FL5_H17
(878 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 67 3e-12
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 51 2e-07
SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual 34 0.023
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 32 0.094
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 29 0.87
SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 28 1.5
SPBC28F2.03 |ppi1|cyp2|cyclophilin family peptidyl-prolyl cis-tr... 27 2.7
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 3.5
SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual 27 4.7
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 26 8.1
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 26 8.1
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 67.3 bits (157), Expect = 3e-12
Identities = 38/122 (31%), Positives = 67/122 (54%)
Frame = +1
Query: 391 NIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEK 570
N+I F EL + C + + AE A +GP+ + LA KY + I+ E++EK
Sbjct: 39 NLILFPELITSGYE-CGNT---FTQIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEK 94
Query: 571 HSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNI 750
S+I++N+ + I++ GN+ G +RK H+ F+ + + + P+F T +GK+ V I
Sbjct: 95 QSNIIYNSCIYITENGNLGGVYRKVHL-----FDTERKHFKKGSDFPIFETSFGKLGVMI 149
Query: 751 CF 756
C+
Sbjct: 150 CW 151
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 50.8 bits (116), Expect = 2e-07
Identities = 50/192 (26%), Positives = 84/192 (43%), Gaps = 5/192 (2%)
Frame = +1
Query: 262 RIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCT 441
R ++G++Q +A D+ N Q + K+++ A + G N+I E++N P+ T
Sbjct: 42 RAFRIGLVQ--LANTKDKSENLQLARL-----KVLEAA-KNGSNVIVLPEIFNSPYG--T 91
Query: 442 REKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 621
+ E E E P+ L +A + + E+ L+NTA+V +G
Sbjct: 92 GYFNQYAEPIE--ESSPSYQALSSMAKDTKTYLFGGSIP--ERKDGKLYNTAMVFDPSGK 147
Query: 622 VIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMM 786
+I HRK H IP F ES+ G+ + T YGK + IC+ M+
Sbjct: 148 LIAVHRKIHLFDIDIPGGVSFRESDSLSPGD-AMTMVDTEYGKFGLGICYDIRFPELAMI 206
Query: 787 FGQNGXXIVFIP 822
+NG ++ P
Sbjct: 207 AARNGCSVMIYP 218
>SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 276
Score = 34.3 bits (75), Expect = 0.023
Identities = 40/170 (23%), Positives = 64/170 (37%), Gaps = 6/170 (3%)
Frame = +1
Query: 355 KKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAM 534
K++I A +G I F E + F E + +RE A K++
Sbjct: 23 KELISQAAAKGAKCIFFPEASD----FIAHNSDEAIELTNHPDCSKFIRDVRESATKHS- 77
Query: 535 VIVSSILERDEKHSDILWNTAVVISDT-GNVIGKHRKNHIPRVGDFN-----ESNYYMEG 696
+ V+ + K + L N+++ I G +I ++ K H+ V N ESN + G
Sbjct: 78 IFVNICVHEPSKVKNKLLNSSLFIEPLHGEIISRYSKAHLFDVEIKNGPTLKESNTTLRG 137
Query: 697 NTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQNGXXIVFIPSXXIAEKG 846
P T GK+ ICF + G I+ PS + G
Sbjct: 138 EAILPPCKTPLGKVGSAICFDIRFPEQAIKLRNMGAHIITYPSAFTEKTG 187
>SPBC336.05c |||S-adenosylmethionine-
dependentmethyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 378
Score = 32.3 bits (70), Expect = 0.094
Identities = 21/64 (32%), Positives = 31/64 (48%)
Frame = -1
Query: 503 KVVVGPSSADSANSHHGCFSLVQNAKGIFHNSWKQMMLTPSWPATSMIFLTLLKIAFFCS 324
++ V S ++A SH CF QN+ + + + +P T F+ LLK AFF
Sbjct: 232 QIAVFHQSKNNAASH--CFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDH 289
Query: 323 LTGR 312
L GR
Sbjct: 290 LFGR 293
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 29.1 bits (62), Expect = 0.87
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = +1
Query: 475 SAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDIL 585
SAE+ + + + +++VS++LE DEKH D++
Sbjct: 979 SAENTTSFSIFAAQGLTDFLIVVSNLLEMDEKHVDVV 1015
>SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1496
Score = 28.3 bits (60), Expect = 1.5
Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
Frame = -3
Query: 285 NYSYLHNSRRSGLLVLGRESVC---GDVEVSLLSCSDRGF 175
++S N++R+G L +G ++VC GD + LSC G+
Sbjct: 885 DFSRSVNNQRNGHLTVGSDAVCLSLGDSQFHRLSCDSVGY 924
>SPBC28F2.03 |ppi1|cyp2|cyclophilin family peptidyl-prolyl cis-trans
isomerase Cyp2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 162
Score = 27.5 bits (58), Expect = 2.7
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = -3
Query: 771 DVVPSEADVHRDLAVSGREYRMAGVTFHVVV 679
DVVP A R L + Y AG TFH V+
Sbjct: 25 DVVPKTAANFRALCTGEKGYGYAGSTFHRVI 55
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.1 bits (57), Expect = 3.5
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -1
Query: 779 QFKTWCRPKQMFTAILPYLVANTGWPVLPS 690
+F T K +FTA+ P+L A T + ++PS
Sbjct: 1521 RFPTRVLCKPVFTAVPPFLFAGTDFALIPS 1550
>SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1202
Score = 26.6 bits (56), Expect = 4.7
Identities = 29/116 (25%), Positives = 48/116 (41%)
Frame = -3
Query: 456 RLLLPCAEREGHIPQLLETDDVNTLLAGNIDDLLDFIENCFLLLVDWTIGGHRDGMLNYS 277
R LL C +R P +L DV ++DD + F+ T GH++ + N S
Sbjct: 119 RALLSCCKRSKD-PSILFPTDVPC----SLDDDVSFL----------TFKGHKNHLENRS 163
Query: 276 YLHNSRRSGLLVLGRESVCGDVEVSLLSCSDRGFFQFDFKVIPPPKMNSVELFQVA 109
+ H+S V+ E + L DR K++ P N ++L +V+
Sbjct: 164 FFHDSESDNFKVVLSNCAINSKEDNNLVTEDR--VNLGAKLLLVPVQNLIKLLKVS 217
>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 502
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 94 NNLTGRDLEEFNRIHFGRRNNLEI 165
NN+ R +EE N I G+R LE+
Sbjct: 8 NNILKRHIEEDNNIDNGKRKKLEL 31
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +1
Query: 64 ETHSLESII-NNNLTGRDLEEFNRIHFGRRNNLEIKLK 174
E H +E +I + N+T DL F + FG+ N ++ K
Sbjct: 369 EFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFK 406
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,454,553
Number of Sequences: 5004
Number of extensions: 72668
Number of successful extensions: 225
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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