BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP04_T7_P14
(798 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 31 0.14
SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity... 31 0.19
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 29 1.0
SPBC4B4.07c |usp102|mud1|U1 snRNP-associated protein Usp102|Schi... 29 1.0
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 28 1.8
SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 27 3.1
SPBC1703.12 |ubp9||ubiquitin C-terminal hydrolase Ubp9|Schizosac... 26 5.4
SPCC306.06c |||ER membrane protein, BIG1 family |Schizosaccharom... 26 7.2
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 26 7.2
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 25 9.5
>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 422
Score = 31.5 bits (68), Expect = 0.14
Identities = 15/66 (22%), Positives = 32/66 (48%)
Frame = -3
Query: 523 PSKVLHFFNTPPDVSDEQLLQVFKDYGVTPPHTISKFPLKSERSSSGLMEFQNISQSVMA 344
PS V++ N P ++++EQ++ +FK G + P + G E+ + + + A
Sbjct: 3 PSCVVYVGNIPYEMAEEQVIDIFKQSGPVKSFQLVIDPESGQPKGYGFCEYHDPATAASA 62
Query: 343 IMACNH 326
+ N+
Sbjct: 63 VRNLNN 68
>SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ctf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 363
Score = 31.1 bits (67), Expect = 0.19
Identities = 15/61 (24%), Positives = 27/61 (44%)
Frame = -3
Query: 499 NTPPDVSDEQLLQVFKDYGVTPPHTISKFPLKSERSSSGLMEFQNISQSVMAIMACNHAT 320
N P DVS++Q+ ++F G + P G EF + + MA+ N++
Sbjct: 13 NIPYDVSEQQMTEIFNQVGPVKTFKLVLDPETGSGKGYGFCEFFDSETTAMAVRKLNNSE 72
Query: 319 I 317
+
Sbjct: 73 L 73
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 28.7 bits (61), Expect = 1.0
Identities = 24/84 (28%), Positives = 37/84 (44%), Gaps = 3/84 (3%)
Frame = -3
Query: 562 LTPASIHKNRIQPPSKVLHFFNTPP--DVSDEQLLQVFKDYGVTPPHTIS-KFPLKSERS 392
LTP HKN+ HFF+ P VS + L+ G++ +TIS F + +
Sbjct: 177 LTPQETHKNKSFERDDQKHFFDRPAFLTVSTQLHLEALA-LGLSRVYTISPAFRAEQSHT 235
Query: 391 SSGLMEFQNISQSVMAIMACNHAT 320
S L EF + V + + + T
Sbjct: 236 SRHLAEFWMLEAEVAFMTSLSQLT 259
>SPBC4B4.07c |usp102|mud1|U1 snRNP-associated protein
Usp102|Schizosaccharomyces pombe|chr 2|||Manual
Length = 249
Score = 28.7 bits (61), Expect = 1.0
Identities = 16/53 (30%), Positives = 31/53 (58%)
Frame = -3
Query: 604 PSFKDYVGNKNNRFLTPASIHKNRIQPPSKVLHFFNTPPDVSDEQLLQVFKDY 446
P+ K V +N ++I+++ + PP+KVL N P +V+ + L Q+F+ +
Sbjct: 146 PTHKKPVPKRNVGAERKSTINEDLL-PPNKVLLLQNIPQEVNADVLTQIFEAF 197
>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1327
Score = 27.9 bits (59), Expect = 1.8
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +2
Query: 527 LYSVLVYRGRR*EAIVLVTHVIFERRTLVGKRI 625
L +V+ + RR AI L V FER T VGK +
Sbjct: 626 LQTVVCTQPRRISAISLAERVAFERDTTVGKEV 658
>SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 561
Score = 27.1 bits (57), Expect = 3.1
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = -3
Query: 559 TPASIHKNRIQPPSKVLHFFNTPPDVSDEQLLQVF 455
TP + + PP ++ N PP S+E+L +F
Sbjct: 412 TPTNTNPADQNPPCNTIYVGNLPPSTSEEELKVLF 446
>SPBC1703.12 |ubp9||ubiquitin C-terminal hydrolase
Ubp9|Schizosaccharomyces pombe|chr 2|||Manual
Length = 585
Score = 26.2 bits (55), Expect = 5.4
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -3
Query: 634 MNPYPLPDKSPSFKDYVGNKNNRFLTPASIHKNR 533
+N YPLP P+FK V K N + +S H +
Sbjct: 69 LNSYPLPSAPPNFKS-VCTKTNHPESSSSRHSKK 101
>SPCC306.06c |||ER membrane protein, BIG1 family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 311
Score = 25.8 bits (54), Expect = 7.2
Identities = 19/58 (32%), Positives = 25/58 (43%), Gaps = 3/58 (5%)
Frame = -3
Query: 469 LLQVFKDYG-VTPPHTISKFPLKSERSSSGLMEFQNISQSVMAIMACNHAT--IQHPG 305
L+ FKD + T SK P S+ SS ++ A+ CN AT I PG
Sbjct: 14 LVSAFKDTSPIFLFSTNSKLPKSSDDSSISTCPMMFFDAAIEALSTCNTATLIIHQPG 71
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 25.8 bits (54), Expect = 7.2
Identities = 22/114 (19%), Positives = 48/114 (42%), Gaps = 6/114 (5%)
Frame = -3
Query: 634 MNPYPLPDKSPSFKDYVGNKNNRFLTPASIHKNRIQPPSKVLHFFNTPPD----VSD--E 473
M+P P+P F ++ +L+P S ++N + S+ + P +S+ +
Sbjct: 270 MSP-PIPPTPSQFSAFMYQNQQSYLSPQSHYENPLSISSRPSPCCPSTPSSAVTLSNGFD 328
Query: 472 QLLQVFKDYGVTPPHTISKFPLKSERSSSGLMEFQNISQSVMAIMACNHATIQH 311
+ + G PP + KFP +S + + Q S+ ++ + ++H
Sbjct: 329 SQSNAYNNSGTGPP-MLYKFPQRSYTAPNTSFNSQRRMSSITSLPTASFCPVKH 381
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -3
Query: 514 VLHFFNTPPDVSDEQLLQ 461
VLHFF T DV D++L +
Sbjct: 819 VLHFFGTMTDVHDQKLAE 836
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,030,479
Number of Sequences: 5004
Number of extensions: 58380
Number of successful extensions: 168
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 389395636
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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