Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BmNP04_T7_O01
         (786 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo...   122   8e-29
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo...   121   1e-28
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    28   1.3  
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||...    28   1.3  
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch...    28   1.3  
SPBC902.06 |mto2||MT organizer Mto2|Schizosaccharomyces pombe|ch...    28   1.7  
SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3|...    27   4.0  
SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp...    25   9.3  

>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  122 bits (293), Expect = 8e-29
 Identities = 71/160 (44%), Positives = 96/160 (60%), Gaps = 2/160 (1%)
 Frame = -1

Query: 750 HRCXIQRKGPLHNFQQGSGFDLAXSATFPGVEXXXXXXXXXXXLAPGGHLGRFVIWTQSA 571
           +R  +QR+GPL  F + +G   A     PGVE           LAPGGHLGRFVIWT+SA
Sbjct: 198 NRRHVQRRGPLVVFNEDAGIVKAFR-NIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSA 256

Query: 570 FGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TR 397
           FG LD +FGS    ++ KKN+ LP+  ++N D+TRL+ SDEI+ +++A    RV RA  +
Sbjct: 257 FGLLDSVFGSTTEAAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQ 316

Query: 396 KLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKALAD 277
           K NPL N   + +LNPYA     KA ++L   K  KA  +
Sbjct: 317 KKNPLKNKAVLARLNPYAKAY--KANVKLNTGKTPKAAGE 354


>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  121 bits (291), Expect = 1e-28
 Identities = 70/160 (43%), Positives = 96/160 (60%), Gaps = 2/160 (1%)
 Frame = -1

Query: 750 HRCXIQRKGPLHNFQQGSGFDLAXSATFPGVEXXXXXXXXXXXLAPGGHLGRFVIWTQSA 571
           +R  +QR+GPL  F + +G   A     PGVE           LAPGGHLGRFVIWT+SA
Sbjct: 198 NRRHVQRRGPLVVFNEDTGIVKAFR-NIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSA 256

Query: 570 FGRLDPLFGSWKTPSKQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TR 397
           FG LD +FGS    ++ KKN+ LP+  ++N D+TRL+ SDEI+ +++A    RV RA  +
Sbjct: 257 FGLLDSVFGSTTEVAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQ 316

Query: 396 KLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKALAD 277
           K NPL N   + +LNPYA     KA +++   K  KA  +
Sbjct: 317 KKNPLKNKAVLSRLNPYAKAY--KANVKINSEKTPKAAGE 354


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
            Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 27/109 (24%), Positives = 46/109 (42%)
 Frame = -1

Query: 525  KQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPY 346
            KQK + +  Q K     LT+   S E + VL       +R T+K   LTN+  ++K +P 
Sbjct: 730  KQKAHLSADQCKQLANVLTQA--SPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSPT 787

Query: 345  AAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLRERRRK 199
              +  ++   +      +  L   ++    L+KRN   K +      RK
Sbjct: 788  PQITVKQNTTKSSSAPRMGMLEQLKQE--YLTKRNFESKLKSSAVSSRK 834


>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1158

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 19/54 (35%), Positives = 33/54 (61%)
 Frame = +1

Query: 343 RVRIEFQHRLVIGERVQFACSTDHAFVGSTEDLPDLIRLEKTCEVSVGHLWLGQ 504
           +V +EF+ RL IG+RV+       AF+GS E +  L+ + +T + ++  L LG+
Sbjct: 207 QVAVEFRKRLNIGDRVKDGLLYKDAFLGS-EAVDVLMHIVRTTDRNLA-LLLGR 258


>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
           Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 19/90 (21%), Positives = 43/90 (47%), Gaps = 2/90 (2%)
 Frame = -1

Query: 459 KSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKA-- 286
           +S  +  +    +K+ IR  R+L+P    ++  + N Y+ + +  ++   R R       
Sbjct: 659 RSRSVTPINNINHKKYIRKDRELSPRGRERSSNR-NSYSDLSRSSSLSRGRSRSYTPEGR 717

Query: 285 LADAEKSGLKLSKRNPAMKAEKLRERRRKN 196
           L ++E  G +    +PA +  + R+R R++
Sbjct: 718 LIESEDKGYRSRSSSPASRKYRSRQRYRRS 747


>SPBC902.06 |mto2||MT organizer Mto2|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 397

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 16/45 (35%), Positives = 23/45 (51%)
 Frame = -2

Query: 602 LDVSSSGLSPHSAGLTPYSGHGRHHRNKRRTSTCPSQRWPTLTSH 468
           +D+ S+ LS H+   T +S   R H      ST PSQ + +  SH
Sbjct: 299 VDLQSNELSHHNVRTTLFSDDSRFHSKIHTHSTPPSQMY-SAASH 342


>SPCC14G10.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 497

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 12/26 (46%), Positives = 14/26 (53%)
 Frame = -2

Query: 596 VSSSGLSPHSAGLTPYSGHGRHHRNK 519
           VS S    HS    P+S  GRH+R K
Sbjct: 120 VSRSSSIGHSGSTAPWSSVGRHNRKK 145


>SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex
           subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 500

 Score = 25.4 bits (53), Expect = 9.3
 Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 1/93 (1%)
 Frame = -1

Query: 477 DLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAM-LKLNPYAAVLKRKAILELRRR 301
           ++T  LK DE+RK+    N +       L  + +++   + L+    + K +  +EL   
Sbjct: 366 EVTETLKGDELRKISLQVNVKFSEEEVTLEDVDDDEIEDILLDKDEILTKTQVWMEL--- 422

Query: 300 KNLKALADAEKSGLKLSKRNPAMKAEKLRERRR 202
            N   LA+ E   LKL +        + R+RRR
Sbjct: 423 -NKDYLAEEEAKNLKLQEDLKKGIVRQPRKRRR 454


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,502,023
Number of Sequences: 5004
Number of extensions: 45461
Number of successful extensions: 140
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 381366860
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)