Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP04_FL5_I24
(848 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 305 4e-84
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 301 6e-83
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 151 9e-38
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 95 8e-21
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 32 0.12
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 28 1.9
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 305 bits (750), Expect = 4e-84
Identities = 136/171 (79%), Positives = 153/171 (89%)
Frame = +2
Query: 332 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 511
+YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDI
Sbjct: 164 EYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDI 223
Query: 512 ERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAE 691
ERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA
Sbjct: 224 ERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAA 283
Query: 692 KAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPXDVNA 844
KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P DV A
Sbjct: 284 KAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQA 334
Score = 157 bits (382), Expect = 1e-39
Identities = 64/88 (72%), Positives = 81/88 (92%)
Frame = +1
Query: 7 FFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIG 186
FFSETG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+G
Sbjct: 56 FFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVG 115
Query: 187 KEIVDLVLDRIRKLADQCTGLQGFLIFH 270
KE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 116 KEMIDSVLERIRRMADNCSGLQGFLVFH 143
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 301 bits (740), Expect = 6e-83
Identities = 134/171 (78%), Positives = 153/171 (89%)
Frame = +2
Query: 332 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 511
+Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDI
Sbjct: 160 EYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDI 219
Query: 512 ERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAE 691
ERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA
Sbjct: 220 ERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAA 279
Query: 692 KAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPXDVNA 844
KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P DV A
Sbjct: 280 KAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQA 330
Score = 160 bits (388), Expect = 3e-40
Identities = 67/88 (76%), Positives = 81/88 (92%)
Frame = +1
Query: 7 FFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIG 186
FFSETG GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+G
Sbjct: 52 FFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVG 111
Query: 187 KEIVDLVLDRIRKLADQCTGLQGFLIFH 270
KE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 112 KELVDEVTDKIRRIADNCSGLQGFLVFH 139
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 151 bits (367), Expect = 9e-38
Identities = 68/170 (40%), Positives = 105/170 (61%)
Frame = +2
Query: 332 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 511
+Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I L I
Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKI 217
Query: 512 ERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAE 691
+ P+Y +LN L+ +++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ +
Sbjct: 218 KSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIG 277
Query: 692 KAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPXDVN 841
+ + +SV E+T F+ N MV DPRHG+Y+ L+RG V +V+
Sbjct: 278 SSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVD 327
Score = 99.5 bits (237), Expect = 5e-22
Identities = 43/88 (48%), Positives = 60/88 (68%)
Frame = +1
Query: 7 FFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIG 186
+F+E GK+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G
Sbjct: 50 YFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEG 109
Query: 187 KEIVDLVLDRIRKLADQCTGLQGFLIFH 270
E+ D VLD +R+ A+ C LQGF + H
Sbjct: 110 AELADAVLDVVRREAEACDALQGFQLTH 137
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 95.5 bits (227), Expect = 8e-21
Identities = 53/174 (30%), Positives = 91/174 (52%), Gaps = 5/174 (2%)
Frame = +2
Query: 335 YGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDI 511
Y KK ++++P Q VS VV+PYNS+L ++D ++DN A+ I L
Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHT 220
Query: 512 ERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS-- 685
+ PT+ N+L+ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P +
Sbjct: 221 QNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQ 280
Query: 686 -AEKAYHEQLSVAEITNACFEPANQMVKCDP-RHGKYMACCMLYRGDVVPXDVN 841
E + +V ++ P NQMV +P + +++ + +G+ P DV+
Sbjct: 281 VEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVH 334
Score = 67.3 bits (157), Expect = 3e-12
Identities = 32/90 (35%), Positives = 55/90 (61%), Gaps = 2/90 (2%)
Frame = +1
Query: 7 FFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 180
FF ++ +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+
Sbjct: 51 FFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 181 IGKEIVDLVLDRIRKLADQCTGLQGFLIFH 270
+ I + ++D I + AD L+GF + H
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLH 139
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 31.9 bits (69), Expect = 0.12
Identities = 17/54 (31%), Positives = 30/54 (55%)
Frame = -2
Query: 637 RHQVSLELGEVHIQSAVESQRSSDRGDNLSNETIQVGVGWALNVEITAADVIDG 476
R + E + H ++ + Q+SSD ++ ++ T VG+G AL I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 27.9 bits (59), Expect = 1.9
Identities = 31/111 (27%), Positives = 47/111 (42%), Gaps = 8/111 (7%)
Frame = -2
Query: 670 VRDQWEVDTGVRHQVSLELGEVHIQSAVESQRSSDRGDNLSNETIQVGVGWALNVEITAA 491
V D VDT V + +A ES R++ D S+ Q + E T A
Sbjct: 454 VADDDAVDTSVTATDDFD-ASASSSNAYESYRNTYTDDMDSSSIYQTSLSNVKTEETTPA 512
Query: 490 DV---IDGFIV-----DHESTVRVLKGCVGGEDRVVGLDDGSGNLRRGVDG 362
+ +DG ++ +HE V+ G E ++ DDGSG +R +DG
Sbjct: 513 EPASKVDGVVLYDFTGEHEG---VITASEGQEFTLLEPDDGSGWVRVKIDG 560
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,469,137
Number of Sequences: 5004
Number of extensions: 72611
Number of successful extensions: 242
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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