Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP03_FL5_I02
(847 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 269 4e-73
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 262 6e-71
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 132 5e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 115 7e-27
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 26 5.8
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.7
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.7
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 26 7.7
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 269 bits (659), Expect = 4e-73
Identities = 118/177 (66%), Positives = 143/177 (80%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 466 RIRKLADQCTGLQGFLIFRXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQV 636
+IR++AD C+GLQGFL+F LL+ERL+++Y KKSKL+F++YPAPQV
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQV 177
Score = 39.9 bits (89), Expect = 4e-04
Identities = 16/24 (66%), Positives = 18/24 (75%)
Frame = +2
Query: 680 HNPLKHSDCAFMVDNEAIYDICRR 751
H L +DC FMVDNE+ YDICRR
Sbjct: 192 HATLDLADCTFMVDNESCYDICRR 215
Score = 32.3 bits (70), Expect = 0.089
Identities = 20/56 (35%), Positives = 24/56 (42%)
Frame = +3
Query: 621 PRASGSTAVVEPYNSIXXXXXXXXXXXXXXXXXXMKPSMTSAAXNLDIERPTYTNL 788
P ST+VVEPYNS+ + NLDIERP+Y NL
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDN-ESCYDICRRNLDIERPSYENL 227
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 262 bits (641), Expect = 6e-71
Identities = 119/182 (65%), Positives = 145/182 (79%), Gaps = 5/182 (2%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 271 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 450
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 451 DLVLDRIRKLADQCTGLQGFLIFRXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 630
D VL+RIR++AD C+GLQGFL+F LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 631 QV 636
QV
Sbjct: 180 QV 181
Score = 44.0 bits (99), Expect = 3e-05
Identities = 18/24 (75%), Positives = 19/24 (79%)
Frame = +2
Query: 680 HNPLKHSDCAFMVDNEAIYDICRR 751
H L +SDC FMVDNEA YDICRR
Sbjct: 196 HATLDNSDCTFMVDNEACYDICRR 219
Score = 33.9 bits (74), Expect = 0.029
Identities = 21/56 (37%), Positives = 24/56 (42%)
Frame = +3
Query: 621 PRASGSTAVVEPYNSIXXXXXXXXXXXXXXXXXXMKPSMTSAAXNLDIERPTYTNL 788
P ST+VVEPYNS+ + NLDIERPTY NL
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDN-EACYDICRRNLDIERPTYENL 231
Score = 26.6 bits (56), Expect = 4.4
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1
Query: 790 NRLIGQIVSSITAS 831
NRLI Q+VSSITAS
Sbjct: 232 NRLIAQVVSSITAS 245
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 132 bits (320), Expect = 5e-32
Identities = 65/176 (36%), Positives = 95/176 (53%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 466 RIRKLADQCTGLQGFLIFRXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 633
+R+ A+ C LQGF + LL+ ++ +Y + F++ PAP+
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 115 bits (277), Expect = 7e-27
Identities = 62/177 (35%), Positives = 98/177 (55%), Gaps = 2/177 (1%)
Frame = +1
Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 289 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 462
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 463 DRIRKLADQCTGLQGFLIFRXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 633
D I + AD L+GF + L+ERL+ Y KK ++++P Q
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2244
Score = 26.2 bits (55), Expect = 5.8
Identities = 11/40 (27%), Positives = 18/40 (45%)
Frame = +2
Query: 584 TTARSLNWSSPSTPRLRFHCRRRALQLYPHHPHNPLKHSD 703
T + W +P+T L + +LY HP +K +D
Sbjct: 220 TAFEDIPWKNPNTENLTSQVSIKEPKLYEPHPTTAIKKAD 259
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.7
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 185 STASSLMARCPQTRPSGVETILSTLSSARPELAST 289
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.7
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 25.8 bits (54), Expect = 7.7
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +2
Query: 665 YPHHPHNPLKHSDCAFMVDNEAIYDI 742
YP HP + L+H D A +NE D+
Sbjct: 714 YPSHPDSLLEHQDHAGDTENEMHDDV 739
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,385,001
Number of Sequences: 5004
Number of extensions: 69617
Number of successful extensions: 233
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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