Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP02_T7_M04
(798 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 225 6e-60
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 225 6e-60
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 225 6e-60
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 67 3e-12
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 47 3e-06
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.11
SPCC417.10 |||membrane transporter|Schizosaccharomyces pombe|chr... 31 0.25
SPCC364.03 |rpl1702|rpl17-2, rpl17|60S ribosomal protein L17|Sch... 28 1.8
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.1
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 26 5.4
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 25 9.5
SPBC2F12.04 |rpl1701|rpl17, rpl17-1|60S ribosomal protein L17|Sc... 25 9.5
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 225 bits (550), Expect = 6e-60
Identities = 102/132 (77%), Positives = 114/132 (86%)
Frame = -1
Query: 591 TVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKEL 412
TVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++
Sbjct: 259 TVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDI 318
Query: 411 RRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 232
RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+D
Sbjct: 319 RRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKID 378
Query: 231 RRTGKSN*SQPK 196
RR+GK PK
Sbjct: 379 RRSGKKIEESPK 390
Score = 97.9 bits (233), Expect = 1e-21
Identities = 46/65 (70%), Positives = 52/65 (80%)
Frame = -2
Query: 200 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAGGGK 21
PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV G K
Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAK 448
Query: 20 VTKAA 6
VTKAA
Sbjct: 449 VTKAA 453
Score = 47.6 bits (108), Expect = 2e-06
Identities = 21/26 (80%), Positives = 21/26 (80%)
Frame = -2
Query: 659 PARPXDKPLRLPLQDVYKIGGXVPCP 582
PARP DKPLRLPLQDVYKIGG P
Sbjct: 236 PARPTDKPLRLPLQDVYKIGGIGTVP 261
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 225 bits (550), Expect = 6e-60
Identities = 102/132 (77%), Positives = 114/132 (86%)
Frame = -1
Query: 591 TVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKEL 412
TVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++
Sbjct: 259 TVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDI 318
Query: 411 RRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 232
RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+D
Sbjct: 319 RRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKID 378
Query: 231 RRTGKSN*SQPK 196
RR+GK PK
Sbjct: 379 RRSGKKIEESPK 390
Score = 97.9 bits (233), Expect = 1e-21
Identities = 46/65 (70%), Positives = 52/65 (80%)
Frame = -2
Query: 200 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAGGGK 21
PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV G K
Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAK 448
Query: 20 VTKAA 6
VTKAA
Sbjct: 449 VTKAA 453
Score = 47.6 bits (108), Expect = 2e-06
Identities = 21/26 (80%), Positives = 21/26 (80%)
Frame = -2
Query: 659 PARPXDKPLRLPLQDVYKIGGXVPCP 582
PARP DKPLRLPLQDVYKIGG P
Sbjct: 236 PARPTDKPLRLPLQDVYKIGGIGTVP 261
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 225 bits (550), Expect = 6e-60
Identities = 102/132 (77%), Positives = 114/132 (86%)
Frame = -1
Query: 591 TVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKEL 412
TVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++
Sbjct: 259 TVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDI 318
Query: 411 RRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 232
RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+D
Sbjct: 319 RRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKID 378
Query: 231 RRTGKSN*SQPK 196
RR+GK PK
Sbjct: 379 RRSGKKIEESPK 390
Score = 97.9 bits (233), Expect = 1e-21
Identities = 46/65 (70%), Positives = 52/65 (80%)
Frame = -2
Query: 200 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAGGGK 21
PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV G K
Sbjct: 389 PKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAPGAAK 448
Query: 20 VTKAA 6
VTKAA
Sbjct: 449 VTKAA 453
Score = 47.6 bits (108), Expect = 2e-06
Identities = 21/26 (80%), Positives = 21/26 (80%)
Frame = -2
Query: 659 PARPXDKPLRLPLQDVYKIGGXVPCP 582
PARP DKPLRLPLQDVYKIGG P
Sbjct: 236 PARPTDKPLRLPLQDVYKIGGIGTVP 261
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 66.9 bits (156), Expect = 3e-12
Identities = 39/127 (30%), Positives = 66/127 (51%), Gaps = 1/127 (0%)
Frame = -1
Query: 591 TVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKE 415
T+ G++E G +K + V+ P N T EV ++ + E + ++ GD V V+ +
Sbjct: 482 TILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SD 540
Query: 414 LRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKV 235
++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++ K+
Sbjct: 541 VQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKL 599
Query: 234 DRRTGKS 214
D+ KS
Sbjct: 600 DKTNRKS 606
Score = 40.7 bits (91), Expect = 2e-04
Identities = 20/53 (37%), Positives = 30/53 (56%), Gaps = 1/53 (1%)
Frame = -2
Query: 200 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 45
P G I L P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 610 PMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 47.2 bits (107), Expect = 3e-06
Identities = 25/67 (37%), Positives = 39/67 (58%), Gaps = 2/67 (2%)
Frame = -1
Query: 591 TVPVGRVETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVK 418
TV GRVE G LK G + V +++ T V +EM + L AV GDN G ++++ +
Sbjct: 268 TVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKRE 327
Query: 417 ELRRGYV 397
+L+RG +
Sbjct: 328 QLKRGMI 334
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.11
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -2
Query: 146 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 54
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
Score = 27.1 bits (57), Expect = 3.1
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -2
Query: 659 PARPXDKPLRLPLQDVYK 606
P +P KPLRL + DVY+
Sbjct: 397 PEKPYRKPLRLSIDDVYR 414
>SPCC417.10 |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 508
Score = 30.7 bits (66), Expect = 0.25
Identities = 19/59 (32%), Positives = 27/59 (45%), Gaps = 2/59 (3%)
Frame = +2
Query: 500 DLTSVVMLAGAKTTMVP--GFNTPVSTLPTGTVXYHRFCIRLAGEDAGACXWGGQVQDG 670
D+ + +M + +P G T S L GT+ Y L G AGAC +GG + G
Sbjct: 289 DIRTWIMFVSSVLLNIPNGGIGTFSSLLIKGTMGYDTLQTLLMGLPAGACEFGGLIAFG 347
>SPCC364.03 |rpl1702|rpl17-2, rpl17|60S ribosomal protein
L17|Schizosaccharomyces pombe|chr 3|||Manual
Length = 187
Score = 27.9 bits (59), Expect = 1.8
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 193 DFGLTSVRFTSTTVNFFFDFCKFA 264
+FG+T R+ +VNFF+D K A
Sbjct: 75 EFGVTQARWPVKSVNFFYDLLKNA 98
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.1
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 412 QFLDGHVLYVETYIVSRYSFLESFV 486
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 26.2 bits (55), Expect = 5.4
Identities = 16/48 (33%), Positives = 19/48 (39%)
Frame = +2
Query: 470 SWRASWCISTDLTSVVMLAGAKTTMVPGFNTPVSTLPTGTVXYHRFCI 613
SW SW +ST L +V + G T ST T RF I
Sbjct: 97 SWNPSWMVSTILVGLVSFMTSDEITTGGIVTSESTRRTYAKDTKRFNI 144
>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1323
Score = 25.4 bits (53), Expect = 9.5
Identities = 19/81 (23%), Positives = 36/81 (44%), Gaps = 8/81 (9%)
Frame = -1
Query: 378 NPPKGAADFTAQVIVLNHPGQISNGY--------TPVLDCHTAHIACKFAEIKEKVDRRT 223
NP K + + +A ++ N P Q++ T +L+ ++H + ++EK + +
Sbjct: 1092 NPKKQSGNTSADQVLFNQPKQVTEKLGVQLNLPKTKILEKVSSHSLAQITALREK-EPKD 1150
Query: 222 GKSN*SQPKIHQVWRCSHCQL 160
S S+ KI H QL
Sbjct: 1151 ADSTYSKEKIFSATSTVHLQL 1171
>SPBC2F12.04 |rpl1701|rpl17, rpl17-1|60S ribosomal protein
L17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 187
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +1
Query: 193 DFGLTSVRFTSTTVNFFFDFCKFA 264
+FG+T R+ +V FF+D K A
Sbjct: 75 EFGVTQARWPVKSVKFFYDLLKNA 98
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,207,344
Number of Sequences: 5004
Number of extensions: 67011
Number of successful extensions: 169
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 389395636
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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