Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP02_T7_M04
(798 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 257 1e-70
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 251 7e-69
EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-a... 202 3e-54
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 141 8e-36
AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr... 23 3.3
L01588-1|AAA27735.1| 74|Apis mellifera zinc finger protein pro... 22 7.6
AY268031-1|AAP23056.1| 810|Apis mellifera dorsal protein splice... 22 7.6
AY268030-1|AAP23055.1| 602|Apis mellifera dorsal protein protein. 22 7.6
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 257 bits (629), Expect = 1e-70
Identities = 119/132 (90%), Positives = 123/132 (93%)
Frame = -1
Query: 591 TVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKEL 412
TVPVGRVETGVLKPG +V FAPA +TTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKEL
Sbjct: 261 TVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKEL 320
Query: 411 RRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 232
RRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA+IKEK D
Sbjct: 321 RRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFADIKEKCD 380
Query: 231 RRTGKSN*SQPK 196
RR GK+ PK
Sbjct: 381 RRNGKTTEENPK 392
Score = 117 bits (282), Expect = 1e-28
Identities = 58/66 (87%), Positives = 61/66 (92%)
Frame = -2
Query: 200 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAGGGK 21
PKSIKSGDAAIV LVPSKP+C E+FQEFPPLGRFAVRDMRQTVAVGVIKAV FK+A GK
Sbjct: 391 PKSIKSGDAAIVMLVPSKPMCAEAFQEFPPLGRFAVRDMRQTVAVGVIKAVTFKDA-AGK 449
Query: 20 VTKAAE 3
VTKAAE
Sbjct: 450 VTKAAE 455
Score = 44.8 bits (101), Expect = 9e-07
Identities = 27/44 (61%), Positives = 27/44 (61%), Gaps = 2/44 (4%)
Frame = -2
Query: 707 EGK*R-KCLXE-L*CHPAPARPXDKPLRLPLQDVYKIGGXVPCP 582
EGK KCL E L P RP DK LRLPLQDVYKIGG P
Sbjct: 220 EGKVEGKCLIEALDAILPPTRPTDKALRLPLQDVYKIGGIGTVP 263
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 251 bits (614), Expect = 7e-69
Identities = 115/132 (87%), Positives = 122/132 (92%)
Frame = -1
Query: 591 TVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKEL 412
TVPVGRVETG+LKPG +V FAPA +TTEVKSVEMHHEAL EA+PGDNVGFNVKN+SVKEL
Sbjct: 261 TVPVGRVETGILKPGMLVTFAPAALTTEVKSVEMHHEALTEALPGDNVGFNVKNISVKEL 320
Query: 411 RRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 232
RRGYVAGDSKN PP+GAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEK D
Sbjct: 321 RRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKCD 380
Query: 231 RRTGKSN*SQPK 196
RRTGK+ PK
Sbjct: 381 RRTGKTTEENPK 392
Score = 111 bits (268), Expect = 5e-27
Identities = 55/66 (83%), Positives = 60/66 (90%)
Frame = -2
Query: 200 PKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAGGGK 21
PKSIKSGDAAIV L P+KP+CVE+FQEFPPLGRFAVRDMRQTVAVGVIK+V FK+ GK
Sbjct: 391 PKSIKSGDAAIVMLQPTKPMCVEAFQEFPPLGRFAVRDMRQTVAVGVIKSVTFKDT-QGK 449
Query: 20 VTKAAE 3
VTKAAE
Sbjct: 450 VTKAAE 455
Score = 43.2 bits (97), Expect = 3e-06
Identities = 19/26 (73%), Positives = 20/26 (76%)
Frame = -2
Query: 659 PARPXDKPLRLPLQDVYKIGGXVPCP 582
P+RP DK LRLPLQDVYKIGG P
Sbjct: 238 PSRPTDKALRLPLQDVYKIGGIGTVP 263
Score = 21.8 bits (44), Expect = 7.6
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +2
Query: 452 LSPGTASWRASWCISTDLTSVVMLAGAKTTMVPGFN 559
L PG A ++T++ SV M A T +PG N
Sbjct: 272 LKPGMLVTFAPAALTTEVKSVEMHHEALTEALPGDN 307
>EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor
1-alpha protein.
Length = 119
Score = 202 bits (493), Expect = 3e-54
Identities = 92/103 (89%), Positives = 96/103 (93%)
Frame = -1
Query: 504 KSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNH 325
KSVEMHHEAL EA+PGDNVGFNVKN+SVKELRRGYVAGDSKN PP+GAADFTAQVIVLNH
Sbjct: 1 KSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNH 60
Query: 324 PGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSN*SQPK 196
PGQISNGYTPVLDCHTAHIACKFAEIKEK DRRTGK+ PK
Sbjct: 61 PGQISNGYTPVLDCHTAHIACKFAEIKEKCDRRTGKTTEENPK 103
Score = 32.3 bits (70), Expect = 0.005
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = -2
Query: 200 PKSIKSGDAAIVNLVPSK 147
PKSIKSGDAAIV L P+K
Sbjct: 102 PKSIKSGDAAIVMLQPTK 119
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 141 bits (341), Expect = 8e-36
Identities = 66/71 (92%), Positives = 68/71 (95%)
Frame = -1
Query: 591 TVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKEL 412
TVPVGRVETGVLKPG +V FAPA +TTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKEL
Sbjct: 204 TVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKEL 263
Query: 411 RRGYVAGDSKN 379
RRGYVAGDSKN
Sbjct: 264 RRGYVAGDSKN 274
Score = 44.8 bits (101), Expect = 9e-07
Identities = 27/44 (61%), Positives = 27/44 (61%), Gaps = 2/44 (4%)
Frame = -2
Query: 707 EGK*R-KCLXE-L*CHPAPARPXDKPLRLPLQDVYKIGGXVPCP 582
EGK KCL E L P RP DK LRLPLQDVYKIGG P
Sbjct: 163 EGKVEGKCLIEALDAILPPTRPTDKALRLPLQDVYKIGGIGTVP 206
>AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor
protein.
Length = 1370
Score = 23.0 bits (47), Expect = 3.3
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -1
Query: 426 SVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPG 319
S ++LR ++A + + PKG Q++VLN G
Sbjct: 283 STRDLREIHLAYNGLRDLPKGIFTRLEQLLVLNLAG 318
>L01588-1|AAA27735.1| 74|Apis mellifera zinc finger protein
protein.
Length = 74
Score = 21.8 bits (44), Expect = 7.6
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = -3
Query: 307 RLHTSLGLPHCPHC 266
RLHT HC HC
Sbjct: 30 RLHTGEKPYHCSHC 43
>AY268031-1|AAP23056.1| 810|Apis mellifera dorsal protein splice
variant B protein.
Length = 810
Score = 21.8 bits (44), Expect = 7.6
Identities = 18/63 (28%), Positives = 24/63 (38%)
Frame = +1
Query: 223 STTVNFFFDFCKFAGNVGSVAIQDWCVTV*DLTRMVKHNDLSCKICSTLRWVVFGVTSNI 402
ST+ N F K G G + CVT R HN + + C V + N+
Sbjct: 77 STSENKTFPTIKIVGYKGRALVVVSCVTKDQPYRPHPHNLVGKEACKQGVCTVEVSSENM 136
Query: 403 TTT 411
T T
Sbjct: 137 TVT 139
>AY268030-1|AAP23055.1| 602|Apis mellifera dorsal protein protein.
Length = 602
Score = 21.8 bits (44), Expect = 7.6
Identities = 18/63 (28%), Positives = 24/63 (38%)
Frame = +1
Query: 223 STTVNFFFDFCKFAGNVGSVAIQDWCVTV*DLTRMVKHNDLSCKICSTLRWVVFGVTSNI 402
ST+ N F K G G + CVT R HN + + C V + N+
Sbjct: 77 STSENKTFPTIKIVGYKGRALVVVSCVTKDQPYRPHPHNLVGKEACKQGVCTVEVSSENM 136
Query: 403 TTT 411
T T
Sbjct: 137 TVT 139
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 214,016
Number of Sequences: 438
Number of extensions: 4719
Number of successful extensions: 18
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 25246416
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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