Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP02_FL5_M04
(851 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 478 e-137
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 461 e-132
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 359 e-101
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 326 1e-91
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 2e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.5
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.6
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.7
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 478 bits (1178), Expect = e-137
Identities = 226/236 (95%), Positives = 228/236 (96%)
Frame = +2
Query: 68 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 247
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 248 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 427
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 428 GEFEAGISKNGQTRERALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 607
GEFEAGISKNGQTRE ALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 608 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVXRKESKXDGKCLIEALDAIL 775
IGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW V RKE K +GKCLIEALDAIL
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAIL 236
Score = 29.5 bits (63), Expect = 0.041
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +3
Query: 777 PPGRPTDKPLRXPLQ 821
PP RPTDK LR PLQ
Sbjct: 237 PPTRPTDKALRLPLQ 251
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 461 bits (1136), Expect = e-132
Identities = 217/236 (91%), Positives = 223/236 (94%)
Frame = +2
Query: 68 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 247
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 248 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 427
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 428 GEFEAGISKNGQTRERALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 607
GEFEAGISKNGQTRE ALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 608 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVXRKESKXDGKCLIEALDAIL 775
IGYN A+VAFVPISGWHGDNMLEPS K PW+KGW+V RK+ DGK LIEALDAIL
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAIL 236
Score = 30.3 bits (65), Expect = 0.024
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +3
Query: 777 PPGRPTDKPLRXPLQ 821
PP RPTDK LR PLQ
Sbjct: 237 PPSRPTDKALRLPLQ 251
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 359 bits (884), Expect = e-101
Identities = 170/179 (94%), Positives = 172/179 (96%)
Frame = +2
Query: 239 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 418
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 419 AGTGEFEAGISKNGQTRERALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 598
AGTGEFEAGISKNGQTRE ALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 599 IKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVXRKESKXDGKCLIEALDAIL 775
IKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW V RKE K +GKCLIEALDAIL
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAIL 179
Score = 29.5 bits (63), Expect = 0.041
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +3
Query: 777 PPGRPTDKPLRXPLQ 821
PP RPTDK LR PLQ
Sbjct: 180 PPTRPTDKALRLPLQ 194
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 326 bits (802), Expect = 1e-91
Identities = 154/163 (94%), Positives = 156/163 (95%)
Frame = +2
Query: 287 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 466
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 467 RERALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 646
RE ALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 647 SGWHGDNMLEPSTKMPWFKGWQVXRKESKXDGKCLIEALDAIL 775
SGWHGDNMLE S+KMPWFKGW V RKE K +GKCLIEALDAIL
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAIL 163
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.5 bits (83), Expect = 2e-04
Identities = 36/117 (30%), Positives = 52/117 (44%)
Frame = +2
Query: 326 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRERALLAFTLGVK 505
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 506 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 676
+IV +NK+D + ++E K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.67
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 80 KTHINIVVIGHVDSGKST 133
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.5
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2
Query: 89 INIVVIGHVDSGKST 133
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.6
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 311 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 412
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.7
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 311 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 412
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 238,273
Number of Sequences: 438
Number of extensions: 5097
Number of successful extensions: 20
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 27431202
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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