Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_T7_I04
(793 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 172 4e-44
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 172 4e-44
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 172 4e-44
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 54 3e-08
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 41 2e-04
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 31 0.14
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 27 2.3
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 3.1
SPCC965.10 |||transcription factor |Schizosaccharomyces pombe|ch... 26 5.4
SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 26 5.4
SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual 26 7.1
SPCC417.10 |||membrane transporter|Schizosaccharomyces pombe|chr... 26 7.1
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 9.4
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 172 bits (419), Expect = 4e-44
Identities = 89/164 (54%), Positives = 104/164 (63%), Gaps = 1/164 (0%)
Frame = -1
Query: 619 RRFGYNPAAVAFVPISGWHGDNMLEPSTKMPLVQGMAGGA*-GRQS*RKMPH*SSRCHPA 443
++ G+NP V FVP+SG+ GDNM+EP+T MP QG G + + P
Sbjct: 177 KKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPP 236
Query: 442 TCPPH*QAPCVFPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHH 263
P P PLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHH
Sbjct: 237 ARPT--DKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHH 294
Query: 262 EALQEAVPGDNVGFNVKNVSVQGIASWLCCW*LQKQPT*GCCRF 131
E+L +PGDNVGFNVKNVSV+ I C + P GC F
Sbjct: 295 ESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF 338
Score = 99.1 bits (236), Expect = 6e-22
Identities = 59/149 (39%), Positives = 78/149 (52%), Gaps = 2/149 (1%)
Frame = -2
Query: 528 PWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPPASSPCKTYTK--SVVLVPCPSA 355
PW++GWQ E K G GK L+EA+D+I PPARPTDKP P + K + VP
Sbjct: 207 PWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKP-LRLPLQDVYKIGGIGTVPVGRV 265
Query: 354 ELKLVC*NQVXXXXXXXXXXXXXXXLWRCTTKLSKKLYLETM*VST*RTCPXKELRRGYV 175
E ++ + + L L + + + + K++RRG V
Sbjct: 266 ETGVIKPGMIVTFAPAGVTTEVKSVEMHHES-LDAGLPGDNVGFNV-KNVSVKDIRRGNV 323
Query: 174 AGDSKNNPPKGAADFTAQVIVLNHPGQIS 88
GDSKN+PP G A FTAQVI+LNHPGQIS
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQIS 352
Score = 47.6 bits (108), Expect = 2e-06
Identities = 37/106 (34%), Positives = 51/106 (48%), Gaps = 1/106 (0%)
Frame = -3
Query: 719 QQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIWLQPSCCRFRAHFWMARRQHVGAF 540
+QLIV VNKMD+T +S+ RFEEI KE S++IKK+ P F +
Sbjct: 146 KQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPT 203
Query: 539 NQNALGSRDGRWSVRKAKLTENASLKLSMPSCHLPA-PLTSPLRLP 405
+ G KA + + +L ++ S PA P PLRLP
Sbjct: 204 TN--MPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLP 247
Score = 44.8 bits (101), Expect = 1e-05
Identities = 17/18 (94%), Positives = 18/18 (100%)
Frame = -3
Query: 83 GYTPVLDCHTAHIACKFA 30
GY+PVLDCHTAHIACKFA
Sbjct: 354 GYSPVLDCHTAHIACKFA 371
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 172 bits (419), Expect = 4e-44
Identities = 89/164 (54%), Positives = 104/164 (63%), Gaps = 1/164 (0%)
Frame = -1
Query: 619 RRFGYNPAAVAFVPISGWHGDNMLEPSTKMPLVQGMAGGA*-GRQS*RKMPH*SSRCHPA 443
++ G+NP V FVP+SG+ GDNM+EP+T MP QG G + + P
Sbjct: 177 KKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPP 236
Query: 442 TCPPH*QAPCVFPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHH 263
P P PLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHH
Sbjct: 237 ARPT--DKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHH 294
Query: 262 EALQEAVPGDNVGFNVKNVSVQGIASWLCCW*LQKQPT*GCCRF 131
E+L +PGDNVGFNVKNVSV+ I C + P GC F
Sbjct: 295 ESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF 338
Score = 99.1 bits (236), Expect = 6e-22
Identities = 59/149 (39%), Positives = 78/149 (52%), Gaps = 2/149 (1%)
Frame = -2
Query: 528 PWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPPASSPCKTYTK--SVVLVPCPSA 355
PW++GWQ E K G GK L+EA+D+I PPARPTDKP P + K + VP
Sbjct: 207 PWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKP-LRLPLQDVYKIGGIGTVPVGRV 265
Query: 354 ELKLVC*NQVXXXXXXXXXXXXXXXLWRCTTKLSKKLYLETM*VST*RTCPXKELRRGYV 175
E ++ + + L L + + + + K++RRG V
Sbjct: 266 ETGVIKPGMIVTFAPAGVTTEVKSVEMHHES-LDAGLPGDNVGFNV-KNVSVKDIRRGNV 323
Query: 174 AGDSKNNPPKGAADFTAQVIVLNHPGQIS 88
GDSKN+PP G A FTAQVI+LNHPGQIS
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQIS 352
Score = 47.6 bits (108), Expect = 2e-06
Identities = 37/106 (34%), Positives = 51/106 (48%), Gaps = 1/106 (0%)
Frame = -3
Query: 719 QQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIWLQPSCCRFRAHFWMARRQHVGAF 540
+QLIV VNKMD+T +S+ RFEEI KE S++IKK+ P F +
Sbjct: 146 KQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPT 203
Query: 539 NQNALGSRDGRWSVRKAKLTENASLKLSMPSCHLPA-PLTSPLRLP 405
+ G KA + + +L ++ S PA P PLRLP
Sbjct: 204 TN--MPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLP 247
Score = 44.8 bits (101), Expect = 1e-05
Identities = 17/18 (94%), Positives = 18/18 (100%)
Frame = -3
Query: 83 GYTPVLDCHTAHIACKFA 30
GY+PVLDCHTAHIACKFA
Sbjct: 354 GYSPVLDCHTAHIACKFA 371
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 172 bits (419), Expect = 4e-44
Identities = 89/164 (54%), Positives = 104/164 (63%), Gaps = 1/164 (0%)
Frame = -1
Query: 619 RRFGYNPAAVAFVPISGWHGDNMLEPSTKMPLVQGMAGGA*-GRQS*RKMPH*SSRCHPA 443
++ G+NP V FVP+SG+ GDNM+EP+T MP QG G + + P
Sbjct: 177 KKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPP 236
Query: 442 TCPPH*QAPCVFPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHH 263
P P PLQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHH
Sbjct: 237 ARPT--DKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHH 294
Query: 262 EALQEAVPGDNVGFNVKNVSVQGIASWLCCW*LQKQPT*GCCRF 131
E+L +PGDNVGFNVKNVSV+ I C + P GC F
Sbjct: 295 ESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF 338
Score = 99.1 bits (236), Expect = 6e-22
Identities = 59/149 (39%), Positives = 78/149 (52%), Gaps = 2/149 (1%)
Frame = -2
Query: 528 PWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPPASSPCKTYTK--SVVLVPCPSA 355
PW++GWQ E K G GK L+EA+D+I PPARPTDKP P + K + VP
Sbjct: 207 PWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKP-LRLPLQDVYKIGGIGTVPVGRV 265
Query: 354 ELKLVC*NQVXXXXXXXXXXXXXXXLWRCTTKLSKKLYLETM*VST*RTCPXKELRRGYV 175
E ++ + + L L + + + + K++RRG V
Sbjct: 266 ETGVIKPGMIVTFAPAGVTTEVKSVEMHHES-LDAGLPGDNVGFNV-KNVSVKDIRRGNV 323
Query: 174 AGDSKNNPPKGAADFTAQVIVLNHPGQIS 88
GDSKN+PP G A FTAQVI+LNHPGQIS
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQIS 352
Score = 47.6 bits (108), Expect = 2e-06
Identities = 37/106 (34%), Positives = 51/106 (48%), Gaps = 1/106 (0%)
Frame = -3
Query: 719 QQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIWLQPSCCRFRAHFWMARRQHVGAF 540
+QLIV VNKMD+T +S+ RFEEI KE S++IKK+ P F +
Sbjct: 146 KQLIVAVNKMDTTG--WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPT 203
Query: 539 NQNALGSRDGRWSVRKAKLTENASLKLSMPSCHLPA-PLTSPLRLP 405
+ G KA + + +L ++ S PA P PLRLP
Sbjct: 204 TN--MPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLP 247
Score = 44.8 bits (101), Expect = 1e-05
Identities = 17/18 (94%), Positives = 18/18 (100%)
Frame = -3
Query: 83 GYTPVLDCHTAHIACKFA 30
GY+PVLDCHTAHIACKFA
Sbjct: 354 GYSPVLDCHTAHIACKFA 371
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 53.6 bits (123), Expect = 3e-08
Identities = 28/73 (38%), Positives = 42/73 (57%), Gaps = 2/73 (2%)
Frame = -1
Query: 418 PCVFPLQDVYKIGGIGTVPVGRVETGVLKPGT--IVVFAPANITTEVKSVEMHHEALQEA 245
P + ++DV+ I G GTV GRVE G LK G +V +++ T V +EM + L A
Sbjct: 252 PFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAA 311
Query: 244 VPGDNVGFNVKNV 206
V GDN G ++++
Sbjct: 312 VAGDNCGLLLRSI 324
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 41.1 bits (92), Expect = 2e-04
Identities = 22/71 (30%), Positives = 38/71 (53%), Gaps = 1/71 (1%)
Frame = -1
Query: 421 APCVFPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEA 245
AP + P+ YK +GT+ G++E G +K + V+ P N T EV ++ + E + +
Sbjct: 467 APFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSS 524
Query: 244 VPGDNVGFNVK 212
+ GD V V+
Sbjct: 525 ICGDQVRLRVR 535
Score = 32.3 bits (70), Expect = 0.082
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -2
Query: 792 GEFEAGIXKXGQTREHA 742
GEFEAG + GQTREHA
Sbjct: 352 GEFEAGFERGGQTREHA 368
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.5 bits (68), Expect = 0.14
Identities = 15/35 (42%), Positives = 24/35 (68%)
Frame = -3
Query: 716 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKI 612
+++V VNK+D +SE RF+EIK VS ++ K+
Sbjct: 317 EIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKM 349
Score = 26.6 bits (56), Expect = 4.1
Identities = 31/124 (25%), Positives = 45/124 (36%), Gaps = 1/124 (0%)
Frame = -2
Query: 786 FEAGIXKXGQTREHAXXL-SPSVSTAHRRSKQNGFH*TTIQ*AQI*GNQEGSILIHQEDL 610
FE G + GQTREHA L + +S + + Q N LI
Sbjct: 293 FERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMSWSEDRFQEIKNIVSDFLIKMVGF 352
Query: 609 ATTQLLSLSCPFLDGTETTCWSLQPKCPWFKGWQVERKEGKADGKCLIEALDAILPPARP 430
T+ + + + GT W+K G L+ ALD ++PP +P
Sbjct: 353 KTSNVHFVPISAISGTNLIQKDSSDLYKWYK------------GPTLLSALDQLVPPEKP 400
Query: 429 TDKP 418
KP
Sbjct: 401 YRKP 404
>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1323
Score = 27.5 bits (58), Expect = 2.3
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = -2
Query: 156 NPPKGAADFTAQVIVLNHPGQISNXLHTSLGLP 58
NP K + + +A ++ N P Q++ L L LP
Sbjct: 1092 NPKKQSGNTSADQVLFNQPKQVTEKLGVQLNLP 1124
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 3.1
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +3
Query: 285 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 401
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPCC965.10 |||transcription factor |Schizosaccharomyces pombe|chr
3|||Manual
Length = 525
Score = 26.2 bits (55), Expect = 5.4
Identities = 13/52 (25%), Positives = 24/52 (46%), Gaps = 3/52 (5%)
Frame = -3
Query: 332 TRYHCCLCPRQHHY*SQV---CGDAPRSSPRSCTWRQCRFQRKERVRXRNCV 186
+RY C +C +V CG ++ + C W+Q +RK + + R +
Sbjct: 17 SRYGCLICRSMRKKCDEVHPQCGRCLKAG-KQCIWKQPGTERKNKTKWRKAM 67
>SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase
Cdc7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1062
Score = 26.2 bits (55), Expect = 5.4
Identities = 17/58 (29%), Positives = 25/58 (43%)
Frame = -3
Query: 584 RAHFWMARRQHVGAFNQNALGSRDGRWSVRKAKLTENASLKLSMPSCHLPAPLTSPLR 411
R H +G+ +GS+D S + L+E+ SLK + H PL LR
Sbjct: 515 RTHLASLLSSLLGSLRDKNIGSKDTTVSQIASILSEDLSLKREIIQAHGILPLLETLR 572
>SPAC12G12.13c |cid14||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 684
Score = 25.8 bits (54), Expect = 7.1
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = -2
Query: 531 CPWFKGWQVERKEGKADGKCLIEALDAILP 442
CPW + ++VER+ + + ++ +D I P
Sbjct: 225 CPWHRQYKVEREVSRIFHQDILHFIDYITP 254
>SPCC417.10 |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 508
Score = 25.8 bits (54), Expect = 7.1
Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 2/58 (3%)
Frame = +3
Query: 279 DLTSVVMLAGAKTTMVP--GFNTPVSTLPTGTVPIPPILYTSCRGKTQGACQWGGQVA 446
D+ + +M + +P G T S L GT+ L T G GAC++GG +A
Sbjct: 289 DIRTWIMFVSSVLLNIPNGGIGTFSSLLIKGTMGYDT-LQTLLMGLPAGACEFGGLIA 345
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 9.4
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +3
Query: 336 NTPVSTLPTGTVPIPPIL 389
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,545,589
Number of Sequences: 5004
Number of extensions: 79350
Number of successful extensions: 272
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 237
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 262
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 385381248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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