Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_T7_F14
(800 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g30450.1 68416.m03852 hypothetical protein similar to At2g049... 33 0.22
At5g23080.1 68418.m02698 SWAP (Suppressor-of-White-APricot)/surp... 31 0.89
At4g16820.1 68417.m02539 lipase class 3 family protein similar t... 31 1.2
At5g40450.1 68418.m04905 expressed protein 30 1.6
At3g30640.1 68416.m03878 Ulp1 protease family protein contains P... 30 1.6
At4g14290.1 68417.m02202 expressed protein contains Interpro ent... 30 2.1
At2g14140.1 68415.m01575 hypothetical protein similar to At2g049... 30 2.1
At5g34895.1 68418.m04113 hypothetical protein similar to At2g049... 29 2.7
At5g25500.1 68418.m03034 expressed protein ; expression supporte... 29 2.7
At5g25520.2 68418.m03037 transcription elongation factor-related... 29 3.6
At5g25520.1 68418.m03036 transcription elongation factor-related... 29 3.6
At4g35170.1 68417.m05000 hypothetical protein predicted protein,... 29 4.7
At2g33460.1 68415.m04101 p21-rho-binding domain-containing prote... 28 6.3
At1g49340.2 68414.m05531 phosphatidylinositol 3- and 4-kinase fa... 28 6.3
At1g49340.1 68414.m05530 phosphatidylinositol 3- and 4-kinase fa... 28 6.3
At5g18510.1 68418.m02185 hypothetical protein 28 8.3
At5g10190.1 68418.m01179 transporter-related low similarity to s... 28 8.3
At4g26330.1 68417.m03786 subtilase family protein contains simil... 28 8.3
At2g28310.2 68415.m03438 expressed protein contains Pfam profile... 28 8.3
At2g28310.1 68415.m03437 expressed protein contains Pfam profile... 28 8.3
At1g18670.1 68414.m02330 protein kinase family protein contains ... 28 8.3
At1g08040.1 68414.m00878 expressed protein contains Pfam profile... 28 8.3
>At3g30450.1 68416.m03852 hypothetical protein similar to At2g04970,
At2g15200, At1g32830, At2g14140, At4g03990, At5g34895,
At3g47270, At2g02200
Length = 800
Score = 33.1 bits (72), Expect = 0.22
Identities = 39/150 (26%), Positives = 56/150 (37%), Gaps = 5/150 (3%)
Frame = -2
Query: 580 EPVKIDTRPVSVTSTSNKTELVGAPKRMTVAELFLKESEQNKNEGVQVTETINKFDKIDL 401
E K++ R T +TE PK+ E+ E E+ K E V+ + N D D
Sbjct: 478 EQEKVEYRDHHSTCNVEETEKQENPKQGD-EEM---EREEGKEEKVEKHDEYN--DAADQ 531
Query: 400 YKSIFLSDSEDEDVGQTKNNDRDFADAPKNLERNNSPPRGIFANIDFHEINSWKRPDKD- 224
I LSD ED D T+ + + L+ N + E D+D
Sbjct: 532 EAYINLSDDEDNDTAPTEKESQQKKEETNVLKEENVEEHDEHDETEDQEAYVILSDDEDN 591
Query: 223 ----VHEQKPKDADGQTEVDKVENVPAPDE 146
++ + TEV K ENV DE
Sbjct: 592 GTAPTEKESQPQKEETTEVPKEENVEEHDE 621
>At5g23080.1 68418.m02698 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 930
Score = 31.1 bits (67), Expect = 0.89
Identities = 18/54 (33%), Positives = 27/54 (50%)
Frame = -2
Query: 523 ELVGAPKRMTVAELFLKESEQNKNEGVQVTETINKFDKIDLYKSIFLSDSEDED 362
+ V A V+E + + E + E E N +DLYK+IF DSED++
Sbjct: 679 DTVKAASARQVSESQVPKKETSIEEPEVEVEVENVERPVDLYKAIFSDDSEDDE 732
>At4g16820.1 68417.m02539 lipase class 3 family protein similar to
DEFECTIVE IN ANTHER DEHISCENCE1 [Arabidopsis thaliana]
GI:16215706; contains Pfam profile PF01764: Lipase
Length = 517
Score = 30.7 bits (66), Expect = 1.2
Identities = 16/53 (30%), Positives = 29/53 (54%)
Frame = -2
Query: 454 NEGVQVTETINKFDKIDLYKSIFLSDSEDEDVGQTKNNDRDFADAPKNLERNN 296
++GV+V +N D + IF ++++ GQ++NN R + +ERNN
Sbjct: 371 SKGVKVLRVVNSQDVVTKVPGIF---ADNDKQGQSRNNGRSPGGIMEMVERNN 420
>At5g40450.1 68418.m04905 expressed protein
Length = 2910
Score = 30.3 bits (65), Expect = 1.6
Identities = 20/91 (21%), Positives = 38/91 (41%), Gaps = 4/91 (4%)
Frame = -2
Query: 619 EQVSFSGSKSLNREPVKIDTRP----VSVTSTSNKTELVGAPKRMTVAELFLKESEQNKN 452
E+V K ++E +KID P + T N ++ P+ + E + ESE K
Sbjct: 528 ERVLLEAEKEEDKEEIKIDEEPSLNAIEKAETENVKIVIEEPEIVNNEETSVHESESLKE 587
Query: 451 EGVQVTETINKFDKIDLYKSIFLSDSEDEDV 359
V N + + + + +++ED+
Sbjct: 588 NAEPVEAVKNSDGTEQISREVTVDRAKEEDI 618
>At3g30640.1 68416.m03878 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain
Length = 661
Score = 30.3 bits (65), Expect = 1.6
Identities = 22/77 (28%), Positives = 35/77 (45%)
Frame = -2
Query: 538 TSNKTELVGAPKRMTVAELFLKESEQNKNEGVQVTETINKFDKIDLYKSIFLSDSEDEDV 359
TS K + + K V+E K+ + + +EGV E + K + K + + EDED
Sbjct: 44 TSEKKKKLVKDKDADVSETPAKKQKVSHSEGVHSREKDAQKKKKNKKKEVAVKSDEDED- 102
Query: 358 GQTKNNDRDFADAPKNL 308
Q ++ DA NL
Sbjct: 103 EQDVRGEQSLVDAVSNL 119
>At4g14290.1 68417.m02202 expressed protein contains Interpro entry
IPR000379
Length = 534
Score = 29.9 bits (64), Expect = 2.1
Identities = 18/52 (34%), Positives = 25/52 (48%)
Frame = -2
Query: 199 ADGQTEVDKVENVPAPDETYGPKIPDNFKPNLASQTCKQDVTLEIDSSSSSD 44
A G T D + V ++P N N +S K+ +LE+ SSSSSD
Sbjct: 347 APGSTS-DAINEVRLKRPMSRTEVPSNDPSNQSSSETKEKESLEVSSSSSSD 397
>At2g14140.1 68415.m01575 hypothetical protein similar to At2g04970,
At2g15200, At1g32830, At3g30450, At4g03990, At5g34895,
At3g47270, At2g02200
Length = 847
Score = 29.9 bits (64), Expect = 2.1
Identities = 29/110 (26%), Positives = 45/110 (40%)
Frame = -2
Query: 472 ESEQNKNEGVQVTETINKFDKIDLYKSIFLSDSEDEDVGQTKNNDRDFADAPKNLERNNS 293
E E+ K E V+ + N D D I LSD ED D T+ + P+ E
Sbjct: 605 EREEGKEEKVEEHDEYN--DAADQEAYINLSDDEDNDTAPTEKESQ-----PQKEETTEV 657
Query: 292 PPRGIFANIDFHEINSWKRPDKDVHEQKPKDADGQTEVDKVENVPAPDET 143
P N++ H+ + + D++ + D D T + E+ P ET
Sbjct: 658 PKE---ENVEEHDEHD-ETEDQEAYVILSDDEDNGTAPTEKESQPQKVET 703
>At5g34895.1 68418.m04113 hypothetical protein similar to At2g04970,
At2g15200, At1g32830, At2g14140, At3g30450, At4g03990,
At3g47270, At2g02200
Length = 490
Score = 29.5 bits (63), Expect = 2.7
Identities = 27/110 (24%), Positives = 45/110 (40%)
Frame = -2
Query: 472 ESEQNKNEGVQVTETINKFDKIDLYKSIFLSDSEDEDVGQTKNNDRDFADAPKNLERNNS 293
E E+ K E V+ N D D + LSD+ED T+ + P+ E
Sbjct: 248 EREEGKEEKVEEHNEYN--DAADQEAYVILSDNEDNGTAPTEKESQ-----PQKEETTEV 300
Query: 292 PPRGIFANIDFHEINSWKRPDKDVHEQKPKDADGQTEVDKVENVPAPDET 143
P N++ H+ + + D++ + D D T + E+ P +ET
Sbjct: 301 PKE---ENVEEHDEHD-ETEDQEAYVILSDDEDNGTAPTEKESQPQKEET 346
>At5g25500.1 68418.m03034 expressed protein ; expression supported
by MPSS
Length = 420
Score = 29.5 bits (63), Expect = 2.7
Identities = 14/43 (32%), Positives = 26/43 (60%)
Frame = -3
Query: 423 INSIKSICTNRYFSVTAKTKTSVRLKIMIAISRTRQRIWRGTI 295
+NS+ +C + +V+A+ + VR + + T+ RIWRGT+
Sbjct: 133 VNSLTKVCN--FLNVSAQQRKLVRSTVCSQV--TQYRIWRGTL 171
>At5g25520.2 68418.m03037 transcription elongation factor-related
contains weak similarity to transcription elongation
factors
Length = 997
Score = 29.1 bits (62), Expect = 3.6
Identities = 41/186 (22%), Positives = 72/186 (38%), Gaps = 18/186 (9%)
Frame = -2
Query: 601 GSKSLNREPVKIDTRPVSVTSTSNKTELVGAPKRMTVAELFLKESEQN-----KNEGVQV 437
G + +PV T VS TSN A + + LK+++ N N+G
Sbjct: 462 GEFQVEIDPVDSGTVDVSAEITSNSKPRAKAKSSKSSTKATLKKNDSNDKNIKSNQGTSS 521
Query: 436 TETINKFDKIDLYKSIFLSDSEDEDVG------------QTKNNDRDFADAPKNLERNNS 293
T+ ++ID + + + D E +DVG ++ N++ F ++
Sbjct: 522 AVTLPPTEEIDPMQGLSM-DDEMKDVGFLPPIVSLDEFMESLNSEPPFGSPHEHPPGKED 580
Query: 292 PPRGIFANIDFHEINSWKRPDKDVHEQKPKDADGQTEVDKVENVPAPDETYGPKIP-DNF 116
P + D S R K ++ + +TE++K NV +P G ++ D
Sbjct: 581 PASEKSDSKDGSHSKSPSRSPKQSPKEPSESVSSKTELEKT-NVISPKPDAGDQLDGDVS 639
Query: 115 KPNLAS 98
KP S
Sbjct: 640 KPENTS 645
>At5g25520.1 68418.m03036 transcription elongation factor-related
contains weak similarity to transcription elongation
factors
Length = 735
Score = 29.1 bits (62), Expect = 3.6
Identities = 41/186 (22%), Positives = 72/186 (38%), Gaps = 18/186 (9%)
Frame = -2
Query: 601 GSKSLNREPVKIDTRPVSVTSTSNKTELVGAPKRMTVAELFLKESEQN-----KNEGVQV 437
G + +PV T VS TSN A + + LK+++ N N+G
Sbjct: 462 GEFQVEIDPVDSGTVDVSAEITSNSKPRAKAKSSKSSTKATLKKNDSNDKNIKSNQGTSS 521
Query: 436 TETINKFDKIDLYKSIFLSDSEDEDVG------------QTKNNDRDFADAPKNLERNNS 293
T+ ++ID + + + D E +DVG ++ N++ F ++
Sbjct: 522 AVTLPPTEEIDPMQGLSM-DDEMKDVGFLPPIVSLDEFMESLNSEPPFGSPHEHPPGKED 580
Query: 292 PPRGIFANIDFHEINSWKRPDKDVHEQKPKDADGQTEVDKVENVPAPDETYGPKIP-DNF 116
P + D S R K ++ + +TE++K NV +P G ++ D
Sbjct: 581 PASEKSDSKDGSHSKSPSRSPKQSPKEPSESVSSKTELEKT-NVISPKPDAGDQLDGDVS 639
Query: 115 KPNLAS 98
KP S
Sbjct: 640 KPENTS 645
>At4g35170.1 68417.m05000 hypothetical protein predicted protein,
Arabidopsis thaliana
Length = 163
Score = 28.7 bits (61), Expect = 4.7
Identities = 21/94 (22%), Positives = 35/94 (37%), Gaps = 2/94 (2%)
Frame = -2
Query: 475 KESEQNKNEGVQVTETI--NKFDKIDLYKSIFLSDSEDEDVGQTKNNDRDFADAPKNLER 302
+ S QN + V V + DKI S D GQ N + R
Sbjct: 7 RSSPQNTRKPVYVVHSPPNTDVDKISTGSGFSPFGSPLNDQGQVSNFQHHSVAESSSYPR 66
Query: 301 NNSPPRGIFANIDFHEINSWKRPDKDVHEQKPKD 200
++ P R ++++ H+++ D+D E D
Sbjct: 67 SSGPLRNEYSSVQVHDLDRRTHEDEDYDEMDGPD 100
>At2g33460.1 68415.m04101 p21-rho-binding domain-containing protein
contains Pfam PF00786: P21-Rho-binding domain
Length = 224
Score = 28.3 bits (60), Expect = 6.3
Identities = 19/66 (28%), Positives = 26/66 (39%), Gaps = 2/66 (3%)
Frame = -2
Query: 301 NNSPPRGIFANI-DFHEINSWKRPDKDVHEQKPKDADGQTEVDKVEN-VPAPDETYGPKI 128
N SPPR N E + R ++ H D + V + +PAPD +I
Sbjct: 117 NGSPPRKSSGNAASSDEPSKHSRHNRSAHGSTDSSNDQEPSVRRRRGGIPAPDTEVPNQI 176
Query: 127 PDNFKP 110
PD P
Sbjct: 177 PDGSAP 182
>At1g49340.2 68414.m05531 phosphatidylinositol 3- and 4-kinase family
protein contains similarity to phosphatidylinositol
4-kinase 230 GI:2326227 from [Homo sapiens], from [Bos
taurus] GI:2198791; contains Pfam profiles PF00454:
Phosphatidylinositol 3- and 4-kinase, PF00613:
Phosphoinositide 3-kinase family, accessory domain
Length = 2028
Score = 28.3 bits (60), Expect = 6.3
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = -3
Query: 750 RFNVPXTRWREYRRKTFKSDPKIXYSVFS 664
R + +W EY + F DP+I SV S
Sbjct: 1474 RLKISSEKWTEYAKTAFSVDPRIALSVAS 1502
>At1g49340.1 68414.m05530 phosphatidylinositol 3- and 4-kinase family
protein contains similarity to phosphatidylinositol
4-kinase 230 GI:2326227 from [Homo sapiens], from [Bos
taurus] GI:2198791; contains Pfam profiles PF00454:
Phosphatidylinositol 3- and 4-kinase, PF00613:
Phosphoinositide 3-kinase family, accessory domain
Length = 2028
Score = 28.3 bits (60), Expect = 6.3
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = -3
Query: 750 RFNVPXTRWREYRRKTFKSDPKIXYSVFS 664
R + +W EY + F DP+I SV S
Sbjct: 1474 RLKISSEKWTEYAKTAFSVDPRIALSVAS 1502
>At5g18510.1 68418.m02185 hypothetical protein
Length = 702
Score = 27.9 bits (59), Expect = 8.3
Identities = 22/102 (21%), Positives = 41/102 (40%)
Frame = -2
Query: 469 SEQNKNEGVQVTETINKFDKIDLYKSIFLSDSEDEDVGQTKNNDRDFADAPKNLERNNSP 290
SE+ + E + + N FD +D D +D D+ + + LE +
Sbjct: 436 SEEIQKESTEAFDARNTFDHLD-------KDDDDGDI-DVSTKVPPLSQVVQKLEEGFTA 487
Query: 289 PRGIFANIDFHEINSWKRPDKDVHEQKPKDADGQTEVDKVEN 164
R I +K + DVH ++ ++AD + DK ++
Sbjct: 488 KRRISRM--HRSAKRYKITESDVHMKRAREADAEISTDKEDD 527
>At5g10190.1 68418.m01179 transporter-related low similarity to
spinster membrane proteins from [Drosophila
melanogaster] GI:12003974, GI:12003976, GI:12003972,
GI:12003970; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 488
Score = 27.9 bits (59), Expect = 8.3
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +3
Query: 225 SLSGLFQEFISWKSILAKIPRGGELFLSKFFGASA 329
SL GLF ++ ++ K P GG +FLS+ SA
Sbjct: 271 SLGGLFGGYMG-DTLAKKFPNGGRIFLSQVSSGSA 304
>At4g26330.1 68417.m03786 subtilase family protein contains
similarity to SBT1, a subtilase from tomato plants
GI:1771160 from [Lycopersicon esculentum]
Length = 746
Score = 27.9 bits (59), Expect = 8.3
Identities = 14/54 (25%), Positives = 27/54 (50%)
Frame = +3
Query: 471 SFKNNSATVILLGAPTSSVLFDVDVTETGLVSILTGSRFSDFDPLNETCSFAKL 632
+ + N+ +I +PT + +VD+ T V IL G+R ++ + T K+
Sbjct: 396 AIRANALALIFAASPTRQLAEEVDMIPTVRVDILHGTRIRNYLARSPTVPMVKI 449
>At2g28310.2 68415.m03438 expressed protein contains Pfam profile
PF05212: Protein of unknown function (DUF707)
Length = 374
Score = 27.9 bits (59), Expect = 8.3
Identities = 18/59 (30%), Positives = 27/59 (45%)
Frame = -2
Query: 379 DSEDEDVGQTKNNDRDFADAPKNLERNNSPPRGIFANIDFHEINSWKRPDKDVHEQKPK 203
D ED G K + P+ E PP + A DF+ W P++D+ ++KPK
Sbjct: 61 DKSPEDFGSRKFPEIYVPTNPRGAEL--LPPGIVVAKTDFYLRRLWGEPNEDL-KKKPK 116
>At2g28310.1 68415.m03437 expressed protein contains Pfam profile
PF05212: Protein of unknown function (DUF707)
Length = 374
Score = 27.9 bits (59), Expect = 8.3
Identities = 18/59 (30%), Positives = 27/59 (45%)
Frame = -2
Query: 379 DSEDEDVGQTKNNDRDFADAPKNLERNNSPPRGIFANIDFHEINSWKRPDKDVHEQKPK 203
D ED G K + P+ E PP + A DF+ W P++D+ ++KPK
Sbjct: 61 DKSPEDFGSRKFPEIYVPTNPRGAEL--LPPGIVVAKTDFYLRRLWGEPNEDL-KKKPK 116
>At1g18670.1 68414.m02330 protein kinase family protein contains
Protein kinases ATP-binding region signature,
PROSITE:PS00107 and Serine/Threonine protein kinases
active-site signature, PROSITE:PS00108
Length = 662
Score = 27.9 bits (59), Expect = 8.3
Identities = 19/54 (35%), Positives = 26/54 (48%)
Frame = +3
Query: 441 WTPSFLFCSDSFKNNSATVILLGAPTSSVLFDVDVTETGLVSILTGSRFSDFDP 602
W P F SD+F+ +G T S +F TETG + L RF +F+P
Sbjct: 123 WVP---FRSDAFEKLEK----IGQGTYSSVFRARETETGRIVALKKVRFDNFEP 169
>At1g08040.1 68414.m00878 expressed protein contains Pfam profile
PF05212: Protein of unknown function (DUF707)
Length = 382
Score = 27.9 bits (59), Expect = 8.3
Identities = 19/63 (30%), Positives = 27/63 (42%)
Frame = -2
Query: 391 IFLSDSEDEDVGQTKNNDRDFADAPKNLERNNSPPRGIFANIDFHEINSWKRPDKDVHEQ 212
+F ED+G K+ P E PP I A DF+ W P +D+ ++
Sbjct: 64 LFSGGRSPEDIGSRKSPKIYVPTNPHGAEL--LPPGIIVAETDFYLRRLWGEPSEDL-KK 120
Query: 211 KPK 203
KPK
Sbjct: 121 KPK 123
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,981,680
Number of Sequences: 28952
Number of extensions: 339479
Number of successful extensions: 1142
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1141
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1814318400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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