Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_T7_E22
(805 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 283 1e-76
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 69 4e-12
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 69 4e-12
At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P329... 33 0.29
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 33 0.29
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 31 0.68
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 31 1.2
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 31 1.2
At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family prot... 29 3.6
At1g16410.2 68414.m01962 cytochrome P450, putative similar to gb... 29 3.6
At1g16410.1 68414.m01963 cytochrome P450, putative similar to gb... 29 3.6
At5g47010.1 68418.m05794 RNA helicase, putative similar to type ... 28 6.3
At2g44760.1 68415.m05571 expressed protein 28 8.3
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 283 bits (693), Expect = 1e-76
Identities = 132/201 (65%), Positives = 156/201 (77%)
Frame = -3
Query: 752 DTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMF 573
+ GN+IGKHRKNHIPRVGDFNES YYMEG+TGHPVF T +GKIAVNIC+GRHH LNW+ F
Sbjct: 202 NNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETVFGKIAVNICYGRHHPLNWLAF 261
Query: 572 GQNGAEIVFNPSATIAGEGGSEYMWNVEARNAAITNCYFTAAINRVGYEEFPNEFTSADG 393
G NGAEIVFNPSAT+ GE SE MW +EARNAAI N YF +INRVG E FPN FTS DG
Sbjct: 262 GLNGAEIVFNPSATV-GEL-SEPMWPIEARNAAIANSYFVGSINRVGTEVFPNPFTSGDG 319
Query: 392 KPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDLNLNRQIRDRRCYYMTQRLD 213
KP H D G FYGSS+F PD P LSR +DGLLI+ +DLNL RQ +D+ + MT R +
Sbjct: 320 KPQHNDFGHFYGSSHFSAPDASCTPSLSRYKDGLLISDMDLNLCRQYKDKWGFRMTARYE 379
Query: 212 MYVNSLSKVLELDYKPQVVHE 150
+Y + L+K ++ D+KPQVV +
Sbjct: 380 VYADLLAKYIKPDFKPQVVSD 400
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 68.9 bits (161), Expect = 4e-12
Identities = 53/185 (28%), Positives = 82/185 (44%), Gaps = 5/185 (2%)
Frame = -3
Query: 746 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQ 567
G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC+ +
Sbjct: 141 GTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVL 200
Query: 566 NGAEIVFNPSATIAGEGGSEYM-----WNVEARNAAITNCYFTAAINRVGYEEFPNEFTS 402
GAEI+F P+A I E + + W + A N A NR+G E
Sbjct: 201 QGAEILFYPTA-IGSEPQDQGLDSRDHWRRVMQGHAGANVVPLVASNRIG-----KEIIE 254
Query: 401 ADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDLNLNRQIRDRRCYYMTQ 222
+ P+ FYG+S+ GP G + +L+A DL++ + R + +
Sbjct: 255 TEHGPSQI---TFYGTSFIAGPTGEIVAEADDKSEAVLVAQFDLDMIKSKRQSWGVFRDR 311
Query: 221 RLDMY 207
R D+Y
Sbjct: 312 RPDLY 316
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 68.9 bits (161), Expect = 4e-12
Identities = 53/185 (28%), Positives = 82/185 (44%), Gaps = 5/185 (2%)
Frame = -3
Query: 746 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQ 567
G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC+ +
Sbjct: 114 GTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVL 173
Query: 566 NGAEIVFNPSATIAGEGGSEYM-----WNVEARNAAITNCYFTAAINRVGYEEFPNEFTS 402
GAEI+F P+A I E + + W + A N A NR+G E
Sbjct: 174 QGAEILFYPTA-IGSEPQDQGLDSRDHWRRVMQGHAGANVVPLVASNRIG-----KEIIE 227
Query: 401 ADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDLNLNRQIRDRRCYYMTQ 222
+ P+ FYG+S+ GP G + +L+A DL++ + R + +
Sbjct: 228 TEHGPSQI---TFYGTSFIAGPTGEIVAEADDKSEAVLVAQFDLDMIKSKRQSWGVFRDR 284
Query: 221 RLDMY 207
R D+Y
Sbjct: 285 RPDLY 289
>At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 224
Score = 32.7 bits (71), Expect = 0.29
Identities = 41/164 (25%), Positives = 62/164 (37%), Gaps = 5/164 (3%)
Frame = -3
Query: 746 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQ 567
G +GKHRK +P + + PV+ T GK+ IC+ L
Sbjct: 22 GQFLGKHRKL-MPT--SLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYA 78
Query: 566 NGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAAINRVGYEEFPNE----FTS 402
G E+ P+A +G E W + AI C+ +A + FP+ FT
Sbjct: 79 KGIELYCAPTA----DGSKE--WQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTD 132
Query: 401 ADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDL 270
H + + G S P G G + +GL+ A +DL
Sbjct: 133 WYDDKEHDSI-VSQGGSVIISPLGQVLAGPNFESEGLVTADIDL 175
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 32.7 bits (71), Expect = 0.29
Identities = 41/164 (25%), Positives = 62/164 (37%), Gaps = 5/164 (3%)
Frame = -3
Query: 746 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQ 567
G +GKHRK +P + + PV+ T GK+ IC+ L
Sbjct: 144 GQFLGKHRKL-MPT--SLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYA 200
Query: 566 NGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAAINRVGYEEFPNE----FTS 402
G E+ P+A +G E W + AI C+ +A + FP+ FT
Sbjct: 201 KGIELYCAPTA----DGSKE--WQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTD 254
Query: 401 ADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDL 270
H + + G S P G G + +GL+ A +DL
Sbjct: 255 WYDDKEHDSI-VSQGGSVIISPLGQVLAGPNFESEGLVTADIDL 297
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 31.5 bits (68), Expect = 0.68
Identities = 45/164 (27%), Positives = 65/164 (39%), Gaps = 5/164 (3%)
Frame = -3
Query: 746 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICF-GRHHVLNWMMFG 570
G +GKHRK +P + + +G+T PVF T GKI IC+ R L M+
Sbjct: 155 GLFLGKHRKL-MPTALERCIWGFG-DGST-IPVFDTPIGKIGAAICWENRMPSLRTAMYA 211
Query: 569 QNGAEIVFNPSATIAGEGGSEYMWNVEARNAAITNCYFTAAIN----RVGYEEFPNEFTS 402
+ G EI P+A S W + A+ F + N R Y P S
Sbjct: 212 K-GIEIYCAPTA------DSRETWLASMTHIALEGGCFVLSANQFCRRKDYPSPPEYMFS 264
Query: 401 ADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDL 270
+ D + G S P G+ G + + L+ A +DL
Sbjct: 265 GSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDL 308
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 30.7 bits (66), Expect = 1.2
Identities = 42/164 (25%), Positives = 59/164 (35%), Gaps = 5/164 (3%)
Frame = -3
Query: 746 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQ 567
G +GKHRK +P + + PV+ T GKI IC+ L
Sbjct: 144 GQFLGKHRKV-MPT--SLERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYRTALYA 200
Query: 566 NGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAAINRVGYEEFPNE----FTS 402
G EI P+A + E W + A+ C+ +A EFP F
Sbjct: 201 KGIEIYCAPTADYSLE------WQASMIHIAVEGGCFVLSAHQFCKRREFPEHPDYLFND 254
Query: 401 ADGKPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDL 270
H D + G S P G G + +GL+ A +DL
Sbjct: 255 IVDTKEH-DPTVSGGGSVIISPLGKVLAGPNYESEGLVTADLDL 297
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 30.7 bits (66), Expect = 1.2
Identities = 40/163 (24%), Positives = 63/163 (38%), Gaps = 4/163 (2%)
Frame = -3
Query: 746 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQ 567
G +GKHRK +P + + PV+ T GK+ IC+ L
Sbjct: 137 GQFLGKHRKL-MPT--SLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYA 193
Query: 566 NGAEIVFNPSATIAGEGGSEYMWNVEARNAAIT-NCYFTAAINRVGYEEFPN--EFTSAD 396
G E+ P+A +G E W + AI C+ +A ++FP+ ++ D
Sbjct: 194 KGIELYCAPTA----DGSKE--WQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTD 247
Query: 395 G-KPAHKDLGLFYGSSYFCGPDGVRCPGLSRTRDGLLIAAVDL 270
D + G S P G G + +GL+ A +DL
Sbjct: 248 WYDDKEPDSIVSQGGSVIISPLGQVLAGPNFESEGLITADLDL 290
>At2g03980.1 68415.m00365 GDSL-motif lipase/hydrolase family protein
similar to Anther-specific proline-rich protein APG from
Brassica napus (SP|P40603 ), Arabidopsis thaliana
(GI:22599); contains Pfam profile PF00657:
Lipase/Acylhydrolase with GDSL-like motif
Length = 367
Score = 29.1 bits (62), Expect = 3.6
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
Frame = +1
Query: 463 QFVIAAFLASTFHMYSLP---PSPAIVADGLKTISAPFCPNIIQ--FKTWCRP 606
+F+ +FL S ++ Y L PS V L +++P CPN+ T C+P
Sbjct: 271 KFIKTSFLYSDYYNYMLGLRGPSSNQVGSSLLNVTSPCCPNVYDGGQLTSCKP 323
>At1g16410.2 68414.m01962 cytochrome P450, putative similar to
gb|AF069494 cytochrome P450 from Sinapis alba and is a
member of the PF|00067 Cytochrome P450 family
Length = 423
Score = 29.1 bits (62), Expect = 3.6
Identities = 25/104 (24%), Positives = 43/104 (41%), Gaps = 17/104 (16%)
Frame = -3
Query: 635 YG-KIAVNICFGRHHVLNWMMFGQNG---------AEIVFNPSATIAGEGGSEYM----- 501
YG + + + FGR HV +F +G E++FN + ++Y+
Sbjct: 194 YGYAVTMRMLFGRRHVTKENVFSDDGRLGNAEKHHLEVIFNTLNCLPSFSPADYVERWLR 253
Query: 500 -WNVEARNAAIT-NCYFTAAINRVGYEEFPNEFTSADGKPAHKD 375
WNV+ + +T NC + N +E + GK A +D
Sbjct: 254 GWNVDGQEKRVTENCNIVRSYNNPIIDERVQLWREEGGKAAVED 297
>At1g16410.1 68414.m01963 cytochrome P450, putative similar to
gb|AF069494 cytochrome P450 from Sinapis alba and is a
member of the PF|00067 Cytochrome P450 family
Length = 538
Score = 29.1 bits (62), Expect = 3.6
Identities = 25/104 (24%), Positives = 43/104 (41%), Gaps = 17/104 (16%)
Frame = -3
Query: 635 YG-KIAVNICFGRHHVLNWMMFGQNG---------AEIVFNPSATIAGEGGSEYM----- 501
YG + + + FGR HV +F +G E++FN + ++Y+
Sbjct: 194 YGYAVTMRMLFGRRHVTKENVFSDDGRLGNAEKHHLEVIFNTLNCLPSFSPADYVERWLR 253
Query: 500 -WNVEARNAAIT-NCYFTAAINRVGYEEFPNEFTSADGKPAHKD 375
WNV+ + +T NC + N +E + GK A +D
Sbjct: 254 GWNVDGQEKRVTENCNIVRSYNNPIIDERVQLWREEGGKAAVED 297
>At5g47010.1 68418.m05794 RNA helicase, putative similar to type 1
RNA helicase pNORF1 [Homo sapiens] GI:1885356
Length = 1254
Score = 28.3 bits (60), Expect = 6.3
Identities = 19/66 (28%), Positives = 36/66 (54%), Gaps = 8/66 (12%)
Frame = +3
Query: 105 RWSISAWNRLKLLFIFVYD---LRLV----IQLEYFAETVHVHVQALGHVI-TTSVSDLS 260
RW I + F+F + LRLV ++L Y + VH Q++GHVI T+ +++
Sbjct: 351 RWDIGLNKKRVAYFVFPKEENELRLVPGDELRLRYSGDAVHPSWQSVGHVIKLTAQEEVA 410
Query: 261 VQVQVH 278
++++ +
Sbjct: 411 LELRAN 416
>At2g44760.1 68415.m05571 expressed protein
Length = 504
Score = 27.9 bits (59), Expect = 8.3
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = -3
Query: 704 VGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGQNGAEI 552
VG+ +ESNYY+ P F ++ + V C R V++ + F GA+I
Sbjct: 291 VGEMSESNYYLT-----PTF--KFEQCLVKGCHKRLRVVHTIEFANGGADI 334
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,104,988
Number of Sequences: 28952
Number of extensions: 330752
Number of successful extensions: 975
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 946
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 972
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1824072800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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