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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BmNP01_FL5_N12
         (880 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_14332| Best HMM Match : Ribosomal_L3 (HMM E-Value=8.6e-34)         197   1e-50
SB_51931| Best HMM Match : Ribosomal_L3 (HMM E-Value=0)               196   2e-50
SB_23052| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   2.8  
SB_14298| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   8.7  

>SB_14332| Best HMM Match : Ribosomal_L3 (HMM E-Value=8.6e-34)
          Length = 347

 Score =  197 bits (480), Expect = 1e-50
 Identities = 85/123 (69%), Positives = 103/123 (83%)
 Frame = +2

Query: 29  PACRTENFQHPXHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFIGYKADMTHVVR 208
           P      F+ P HGS+GF P+KR +RHRGKVK+FPKDD + P HLTAFIG+KA MTH++R
Sbjct: 46  PKMSHRKFEAPRHGSLGFLPRKRCKRHRGKVKSFPKDDNTLPPHLTAFIGFKAGMTHILR 105

Query: 209 EPDRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAEHMSEDCRRRF 388
           E ++PGSK+NKKE VEAVTIIETPPM+ VGVVGYIETP G+R L T+WAEH+SE+C+RRF
Sbjct: 106 EVEKPGSKLNKKEKVEAVTIIETPPMMVVGVVGYIETPRGMRVLKTIWAEHLSEECKRRF 165

Query: 389 YKN 397
           YKN
Sbjct: 166 YKN 168



 Score = 48.4 bits (110), Expect = 8e-06
 Identities = 19/31 (61%), Positives = 24/31 (77%)
 Frame = +3

Query: 582 WQDELGRKSIEKDFKKMIRYCSVVRVIAHTQ 674
           W D+ G+KSIE+DF  M +YC V+RVI HTQ
Sbjct: 184 WADDDGKKSIEEDFNTMKKYCKVIRVICHTQ 214



 Score = 28.7 bits (61), Expect = 6.6
 Identities = 13/24 (54%), Positives = 14/24 (58%)
 Frame = +1

Query: 22  LSSSMSHRKFSAPRSWVYGILSQK 93
           L   MSHRKF APR    G L +K
Sbjct: 44  LEPKMSHRKFEAPRHGSLGFLPRK 67


>SB_51931| Best HMM Match : Ribosomal_L3 (HMM E-Value=0)
          Length = 338

 Score =  196 bits (478), Expect = 2e-50
 Identities = 84/116 (72%), Positives = 102/116 (87%)
 Frame = +2

Query: 50  FQHPXHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFIGYKADMTHVVREPDRPGS 229
           F+ P HGS+GF P+KR +RHRGKVK+FPKDD + P HLTAFIG+KA MTH++RE ++PGS
Sbjct: 5   FEAPRHGSLGFLPRKRCKRHRGKVKSFPKDDNTLPPHLTAFIGFKAGMTHILREVEKPGS 64

Query: 230 KINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAEHMSEDCRRRFYKN 397
           K+NKKE VEAVTIIETPPM+ VGVVGYIETP G+R L T+WAEH+SE+C+RRFYKN
Sbjct: 65  KLNKKEKVEAVTIIETPPMMVVGVVGYIETPRGMRVLKTIWAEHLSEECKRRFYKN 120



 Score =  111 bits (267), Expect = 7e-25
 Identities = 54/100 (54%), Positives = 67/100 (67%), Gaps = 2/100 (2%)
 Frame = +3

Query: 582 WQDELGRKSIEKDFKKMIRYCSVVRVIAHTQMKLLKQRQKKAHIMEIQLNGG-TIEDKVK 758
           W D+ G+KSIE+DF  M +YC V+RVI HTQ KLLK RQKKAHIMEIQ+NGG  + +KV 
Sbjct: 136 WADDDGKKSIEEDFNTMKKYCKVIRVICHTQQKLLKMRQKKAHIMEIQVNGGKDVAEKVD 195

Query: 759 WAXXHLEKPIPVDSVLXXMK*LXAL-VSQGQGSKGVXSRW 875
           W    LE P PV  V    + +  + V++G G KGV  RW
Sbjct: 196 WCRERLENPAPVRKVFSPDEMIDVIGVTKGHGFKGVTYRW 235


>SB_23052| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 479

 Score = 29.9 bits (64), Expect = 2.8
 Identities = 17/43 (39%), Positives = 25/43 (58%)
 Frame = +2

Query: 29  PACRTENFQHPXHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPV 157
           P C T+  QHP   S+ F  KK +  ++G  K++ K  P+KPV
Sbjct: 191 PVCDTDGQQHPNLCSLHFQGKKLA--YKGFCKSYCK-SPTKPV 230


>SB_14298| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 427

 Score = 28.3 bits (60), Expect = 8.7
 Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
 Frame = -3

Query: 428 NKALIFREESSFCRSDVDSLQTYAP-PRQSTER 333
           N A+I +    +C  D+   Q YAP  RQ++ R
Sbjct: 392 NDAVILKRRECYCEKDLSKTQLYAPSDRQASNR 424


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 27,109,317
Number of Sequences: 59808
Number of extensions: 575162
Number of successful extensions: 1129
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 1003
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1127
length of database: 16,821,457
effective HSP length: 82
effective length of database: 11,917,201
effective search space used: 2502612210
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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