Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_FL5_N10
(883 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g59990.2 68416.m06698 methionyl aminopeptidase, putative / me... 287 9e-78
At3g59990.1 68416.m06697 methionyl aminopeptidase, putative / me... 287 9e-78
At2g44180.1 68415.m05496 methionyl aminopeptidase, putative / me... 280 8e-76
At3g51800.2 68416.m05681 metallopeptidase M24 family protein sim... 60 3e-09
At3g51800.1 68416.m05680 metallopeptidase M24 family protein sim... 60 3e-09
At4g37040.1 68417.m05246 metallopeptidase M24 family protein sim... 33 0.33
At2g34920.1 68415.m04287 ubiquitin-protein ligase-related contai... 31 1.3
At1g10130.1 68414.m01142 calcium-transporting ATPase 3, endoplas... 29 4.1
At4g36760.2 68417.m05215 aminopeptidase P similar to Xaa-Pro ami... 28 7.2
At4g36760.1 68417.m05216 aminopeptidase P similar to Xaa-Pro ami... 28 7.2
At4g21240.1 68417.m03071 F-box family protein contains F-box dom... 28 9.5
>At3g59990.2 68416.m06698 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to Methionine aminopeptidase 2 (EC
3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo
sapiens} SP|P50579; contains Pfam profile PF00557:
metallopeptidase family M24; supporting cDNA
gi|11344921|gb|AF300880.1|AF300880
Length = 439
Score = 287 bits (703), Expect = 9e-78
Identities = 135/232 (58%), Positives = 167/232 (71%)
Frame = +3
Query: 102 QTVPPTIPVAELFPDGNFPEGQIMDHGPAEGIDERTAKNRFTSEEKRALDRLHKDIYQEI 281
QT PP+IPV ELFP G FPEG+I ++ D+ R TSEEKR L+R K IY +
Sbjct: 78 QTDPPSIPVVELFPSGEFPEGEIQEYK-----DDNLW--RTTSEEKRELERFEKPIYNSV 130
Query: 282 RHAAEAHRQTRKHIRNWIKPGMTMIDICEELEKTARRLIGEDGLKAGLAFPTGCSRNHCA 461
R AAE HRQ RK++R+ +KPGM M DICE LE T R+LI E+GL+AG+AFPTGCS N A
Sbjct: 131 RRAAEVHRQVRKYVRSIVKPGMLMTDICETLENTVRKLISENGLQAGIAFPTGCSLNWVA 190
Query: 462 AHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHFNPRYDPLVKGVQXATEAGIK 641
AH+TPN+GD TVL+YDDV+K+DFGTHI+G IIDCAFT+ FNP +DPL+ + AT GIK
Sbjct: 191 AHWTPNSGDKTVLQYDDVMKLDFGTHIDGHIIDCAFTVAFNPMFDPLLAASREATYTGIK 250
Query: 642 ASGVDVRLCDVXAAVQXVMXSHXXEXXGXMYXXKPXRNLNGHXXEPYXIXXG 797
+G+DVRLCD+ AA+Q VM S+ E G ++ K RNLNGH PY I G
Sbjct: 251 EAGIDVRLCDIGAAIQEVMESYEVEINGKVFQVKSIRNLNGHSIGPYQIHAG 302
>At3g59990.1 68416.m06697 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to Methionine aminopeptidase 2 (EC
3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo
sapiens} SP|P50579; contains Pfam profile PF00557:
metallopeptidase family M24; supporting cDNA
gi|11344921|gb|AF300880.1|AF300880
Length = 439
Score = 287 bits (703), Expect = 9e-78
Identities = 135/232 (58%), Positives = 167/232 (71%)
Frame = +3
Query: 102 QTVPPTIPVAELFPDGNFPEGQIMDHGPAEGIDERTAKNRFTSEEKRALDRLHKDIYQEI 281
QT PP+IPV ELFP G FPEG+I ++ D+ R TSEEKR L+R K IY +
Sbjct: 78 QTDPPSIPVVELFPSGEFPEGEIQEYK-----DDNLW--RTTSEEKRELERFEKPIYNSV 130
Query: 282 RHAAEAHRQTRKHIRNWIKPGMTMIDICEELEKTARRLIGEDGLKAGLAFPTGCSRNHCA 461
R AAE HRQ RK++R+ +KPGM M DICE LE T R+LI E+GL+AG+AFPTGCS N A
Sbjct: 131 RRAAEVHRQVRKYVRSIVKPGMLMTDICETLENTVRKLISENGLQAGIAFPTGCSLNWVA 190
Query: 462 AHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHFNPRYDPLVKGVQXATEAGIK 641
AH+TPN+GD TVL+YDDV+K+DFGTHI+G IIDCAFT+ FNP +DPL+ + AT GIK
Sbjct: 191 AHWTPNSGDKTVLQYDDVMKLDFGTHIDGHIIDCAFTVAFNPMFDPLLAASREATYTGIK 250
Query: 642 ASGVDVRLCDVXAAVQXVMXSHXXEXXGXMYXXKPXRNLNGHXXEPYXIXXG 797
+G+DVRLCD+ AA+Q VM S+ E G ++ K RNLNGH PY I G
Sbjct: 251 EAGIDVRLCDIGAAIQEVMESYEVEINGKVFQVKSIRNLNGHSIGPYQIHAG 302
>At2g44180.1 68415.m05496 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to SP|P50579 Methionine aminopeptidase
2 (EC 3.4.11.18) (MetAP 2) {Homo sapiens}; contains Pfam
profile PF00557: metallopeptidase family M24
Length = 441
Score = 280 bits (687), Expect = 8e-76
Identities = 134/229 (58%), Positives = 167/229 (72%)
Frame = +3
Query: 102 QTVPPTIPVAELFPDGNFPEGQIMDHGPAEGIDERTAKNRFTSEEKRALDRLHKDIYQEI 281
QT PP+IPV ELFP G+FP+G+I + D+ R TSEEKR ++RL K IY +
Sbjct: 80 QTDPPSIPVLELFPSGDFPQGEIQQYN-----DDNLW--RTTSEEKREMERLQKPIYNSL 132
Query: 282 RHAAEAHRQTRKHIRNWIKPGMTMIDICEELEKTARRLIGEDGLKAGLAFPTGCSRNHCA 461
R AAE HRQ RK++R+ +KPGM MID+CE LE T R+LI E+GL+AG+AFPTGCS N+ A
Sbjct: 133 RQAAEVHRQVRKYMRSILKPGMLMIDLCETLENTVRKLISENGLQAGIAFPTGCSLNNVA 192
Query: 462 AHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHFNPRYDPLVKGVQXATEAGIK 641
AH+TPN+GD TVL+YDDV+K+DFGTHI+G I+D AFT+ FNP +DPL+ + AT GIK
Sbjct: 193 AHWTPNSGDKTVLQYDDVMKLDFGTHIDGHIVDSAFTVAFNPMFDPLLAASRDATYTGIK 252
Query: 642 ASGVDVRLCDVXAAVQXVMXSHXXEXXGXMYXXKPXRNLNGHXXEPYXI 788
+GVDVRLCDV AAVQ VM S+ E G +Y K RNLNGH Y I
Sbjct: 253 EAGVDVRLCDVGAAVQEVMESYEVEINGKVYQVKSIRNLNGHSIGRYQI 301
>At3g51800.2 68416.m05681 metallopeptidase M24 family protein
similar to SP|P50580 Proliferation-associated protein
2G4 {Mus musculus}; contains Pfam profile PF00557:
metallopeptidase family M24
Length = 401
Score = 59.7 bits (138), Expect = 3e-09
Identities = 44/169 (26%), Positives = 79/169 (46%), Gaps = 11/169 (6%)
Frame = +3
Query: 234 EKRALDRLHKDIYQEIRHAAEAHRQTRKHIRNWIKPGMTMIDICEE-----LEKTARRLI 398
+++ L ++ + + AAE + + + KP ++DICE+ E+TA
Sbjct: 8 DEKELSLTSPEVVTKYKSAAEIVNKALQVVLAECKPKAKIVDICEKGDSFIKEQTASMYK 67
Query: 399 G-EDGLKAGLAFPTGCSRNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHING--RIIDCAF 569
+ ++ G+AFPT S N+ H++P D +VLE D+VKID G HI+G ++
Sbjct: 68 NSKKKIERGVAFPTCISVNNTVGHFSPLASDESVLEDGDMVKIDMGCHIDGFIALVGHTH 127
Query: 570 TLHFNP---RYDPLVKGVQXATEAGIKASGVDVRLCDVXAAVQXVMXSH 707
L P R ++ A + ++ + DV A+Q V ++
Sbjct: 128 VLQEGPLSGRKADVIAAANTAADVALRLVRPGKKNTDVTEAIQKVAAAY 176
>At3g51800.1 68416.m05680 metallopeptidase M24 family protein
similar to SP|P50580 Proliferation-associated protein
2G4 {Mus musculus}; contains Pfam profile PF00557:
metallopeptidase family M24
Length = 392
Score = 59.7 bits (138), Expect = 3e-09
Identities = 44/169 (26%), Positives = 79/169 (46%), Gaps = 11/169 (6%)
Frame = +3
Query: 234 EKRALDRLHKDIYQEIRHAAEAHRQTRKHIRNWIKPGMTMIDICEE-----LEKTARRLI 398
+++ L ++ + + AAE + + + KP ++DICE+ E+TA
Sbjct: 8 DEKELSLTSPEVVTKYKSAAEIVNKALQVVLAECKPKAKIVDICEKGDSFIKEQTASMYK 67
Query: 399 G-EDGLKAGLAFPTGCSRNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHING--RIIDCAF 569
+ ++ G+AFPT S N+ H++P D +VLE D+VKID G HI+G ++
Sbjct: 68 NSKKKIERGVAFPTCISVNNTVGHFSPLASDESVLEDGDMVKIDMGCHIDGFIALVGHTH 127
Query: 570 TLHFNP---RYDPLVKGVQXATEAGIKASGVDVRLCDVXAAVQXVMXSH 707
L P R ++ A + ++ + DV A+Q V ++
Sbjct: 128 VLQEGPLSGRKADVIAAANTAADVALRLVRPGKKNTDVTEAIQKVAAAY 176
>At4g37040.1 68417.m05246 metallopeptidase M24 family protein
similar to SP|O33343 Methionine aminopeptidase (EC
3.4.11.18) (Peptidase M) {Mycobacterium tuberculosis};
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 350
Score = 32.7 bits (71), Expect = 0.33
Identities = 33/143 (23%), Positives = 59/143 (41%), Gaps = 9/143 (6%)
Frame = +3
Query: 306 QTRKHIRNWIKPGMTMIDICEELEKTARRLIGEDGLKAGL----AFPTG-C-SRNHCAAH 467
+ R + +KPG+T +E+++ +I E+G FP C S N C H
Sbjct: 125 RVRDYAGTLVKPGVTT----DEIDEAVHNMIIENGAYPSPLGYGGFPKSVCTSVNECICH 180
Query: 468 YTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTL---HFNPRYDPLVKGVQXATEAGI 638
P D+ LE D++ ID ++NG D + T + + + LV+ + + + I
Sbjct: 181 GIP---DSRPLEDGDIINIDVTVYLNGYHGDTSATFFCGNVDEKAKKLVEVTKESLDKAI 237
Query: 639 KASGVDVRLCDVXAAVQXVMXSH 707
G V + + + H
Sbjct: 238 SICGPGVEYKKIGKVIHDLADKH 260
>At2g34920.1 68415.m04287 ubiquitin-protein ligase-related contains
weak similarity to Ubiquitin-protein ligase E3 Mdm2 (EC
6.3.2.-) (p53-binding protein Mdm2) (Oncoprotein Mdm2)
(Double minute 2 protein) (Swiss-Prot:P23804) [Mus
musculus]
Length = 785
Score = 30.7 bits (66), Expect = 1.3
Identities = 23/82 (28%), Positives = 34/82 (41%)
Frame = +3
Query: 177 HGPAEGIDERTAKNRFTSEEKRALDRLHKDIYQEIRHAAEAHRQTRKHIRNWIKPGMTMI 356
H E + R A +RF + R L + + HR T K N +P T++
Sbjct: 354 HRELESLLGRNAVSRFP-QRGRLQSMLRLRSLKRGLAIQDRHRGTTKSDLNRFQPSSTIL 412
Query: 357 DICEELEKTARRLIGEDGLKAG 422
+ E+L + A E GLK G
Sbjct: 413 HLREKLREKAANAAAEAGLKKG 434
>At1g10130.1 68414.m01142 calcium-transporting ATPase 3, endoplasmic
reticulum-type (ACA6) (ECA3) nearly identical to
SP|Q9SY55 Calcium-transporting ATPase 3, endoplasmic
reticulum-type (EC 3.6.3.8) {Arabidopsis thaliana);
contains InterPro Accession IPR006069: Cation
transporting ATPase
Length = 998
Score = 29.1 bits (62), Expect = 4.1
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = -3
Query: 326 ADVFSCLPVGFSSMSDFLINIFM*SIKSSFLLTCKTIFCSSLINTLSRTMV 174
A V +CL +G M+ +N + S+ S L C T+ CS TL+ M+
Sbjct: 309 AVVTTCLALGTKKMAR--LNAIVRSLPSVETLGCTTVICSDKTGTLTTNMM 357
>At4g36760.2 68417.m05215 aminopeptidase P similar to Xaa-Pro
aminopeptidase 2 [Lycopersicon esculentum] GI:15384991;
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 519
Score = 28.3 bits (60), Expect = 7.2
Identities = 17/56 (30%), Positives = 23/56 (41%), Gaps = 2/56 (3%)
Frame = +3
Query: 420 GLAFPTGCS--RNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHF 581
GL+FPT S N HY+P ++ D + D G D T+HF
Sbjct: 392 GLSFPTISSVGSNAAVIHYSPEPEACAEMDPDKIYLCDSGAQYLDGTTDITRTVHF 447
>At4g36760.1 68417.m05216 aminopeptidase P similar to Xaa-Pro
aminopeptidase 2 [Lycopersicon esculentum] GI:15384991;
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 634
Score = 28.3 bits (60), Expect = 7.2
Identities = 17/56 (30%), Positives = 23/56 (41%), Gaps = 2/56 (3%)
Frame = +3
Query: 420 GLAFPTGCS--RNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHF 581
GL+FPT S N HY+P ++ D + D G D T+HF
Sbjct: 392 GLSFPTISSVGSNAAVIHYSPEPEACAEMDPDKIYLCDSGAQYLDGTTDITRTVHF 447
>At4g21240.1 68417.m03071 F-box family protein contains F-box domain
Pfam:PF00646
Length = 417
Score = 27.9 bits (59), Expect = 9.5
Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +3
Query: 456 CAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDC-AFTLHFNPRYD 596
C +Y P +G +EY+ + +F IN I++C FT+ + P ++
Sbjct: 358 CVLYYDPKSGSERRIEYEGIGDREF--CINTGILECKRFTIDWFPNHN 403
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,483,802
Number of Sequences: 28952
Number of extensions: 286538
Number of successful extensions: 800
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 777
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 795
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2077687200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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