Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_FL5_M21
(863 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 245 6e-66
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 241 7e-65
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 127 2e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 111 9e-26
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.9
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 245 bits (600), Expect = 6e-66
Identities = 107/161 (66%), Positives = 129/161 (80%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDY 588
+IR++AD C+GLQGFL+FH LL+ERL+++Y
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEY 161
Score = 52.0 bits (119), Expect = 1e-07
Identities = 24/41 (58%), Positives = 29/41 (70%)
Frame = +3
Query: 591 QKSKLEFXIYPAPQVSTAVVXPYNSYPHHHXXLXHSDXAFM 713
+KSKL+F +YPAPQVST+VV PYNS H L +D FM
Sbjct: 163 KKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFM 203
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 241 bits (591), Expect = 7e-65
Identities = 110/171 (64%), Positives = 134/171 (78%), Gaps = 5/171 (2%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 271 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 450
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 451 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGRSLN 603
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YG+ N
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSN 170
Score = 52.8 bits (121), Expect = 6e-08
Identities = 24/41 (58%), Positives = 29/41 (70%)
Frame = +3
Query: 591 QKSKLEFXIYPAPQVSTAVVXPYNSYPHHHXXLXHSDXAFM 713
+KS L+F +YPAPQVST+VV PYNS H L +SD FM
Sbjct: 167 KKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFM 207
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 127 bits (306), Expect = 2e-30
Identities = 62/161 (38%), Positives = 88/161 (54%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDY 588
+R+ A+ C LQGF + H LL+ ++ +Y
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEY 159
Score = 31.5 bits (68), Expect = 0.16
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +3
Query: 609 FXIYPAPQVSTAVVXPYNSYPHHHXXLXHSDXAF 710
F + PAP+ S VV PYN+ H + +SD F
Sbjct: 167 FSVAPAPKSSDTVVEPYNATLSMHQLVENSDETF 200
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 111 bits (268), Expect = 9e-26
Identities = 59/166 (35%), Positives = 93/166 (56%), Gaps = 2/166 (1%)
Frame = +1
Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 289 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 462
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 463 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGRSL 600
D I + AD L+GF + H L+ERL+ Y + +
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKI 165
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.9
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 185 STASSLMARCPQTRPSGVETILSTLSSARPELAST 289
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,079,628
Number of Sequences: 5004
Number of extensions: 61539
Number of successful extensions: 163
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 430470850
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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