BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BmNP01_FL5_M18 (916 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholi... 33 0.004 AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic ac... 33 0.004 AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic ac... 31 0.015 DQ667183-1|ABG75735.1| 463|Apis mellifera GABA-gated ion channe... 27 0.31 DQ667185-1|ABG75737.1| 447|Apis mellifera glutamate-gated chlor... 26 0.42 DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholi... 26 0.42 DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholi... 26 0.55 DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 25 0.73 DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 25 0.73 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 25 0.96 DQ667186-1|ABG75738.1| 447|Apis mellifera glutamate-gated chlor... 23 2.9 >DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholine receptor alpha1subunit protein. Length = 601 Score = 33.1 bits (72), Expect = 0.004 Identities = 22/77 (28%), Positives = 35/77 (45%), Gaps = 2/77 (2%) Frame = +2 Query: 134 RTIGTPPPXSKIVVNLTL-HLRHANIRESESTVRIQADLQMNXIDKRLSXNXGEWG-CST 307 R +G + + L L L N++ T + + + N D +L N ++G T Sbjct: 37 RPVGNNSDRLTVKMGLRLSQLIDVNLKNQIMTTNVWVEQEWN--DYKLKWNPDDYGGVDT 94 Query: 308 WLVSSERLWMPDXVLLN 358 V SE +W+PD VL N Sbjct: 95 LHVPSEHIWLPDIVLYN 111 >AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic acetylcholine receptoralpha-3 protein. Length = 537 Score = 33.1 bits (72), Expect = 0.004 Identities = 22/77 (28%), Positives = 31/77 (40%), Gaps = 1/77 (1%) Frame = +2 Query: 203 NIRESESTVRIQADLQMNXIDKRLSXNXGEWGCSTWL-VSSERLWMPDXVLLNXXXXTXG 379 N++ T + + + N D +L N E+G L V SE +W+PD VL N Sbjct: 74 NLKNQVMTTNVWVEQRWN--DYKLKWNPEEYGGVEMLYVPSENIWLPDIVLYNNADGNYE 131 Query: 380 GLRSPXPRYLXTGSXSW 430 TG SW Sbjct: 132 VTLMTKATLKYTGDVSW 148 >AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic acetylcholine receptorApisa2 subunit protein. Length = 541 Score = 31.1 bits (67), Expect = 0.015 Identities = 26/92 (28%), Positives = 37/92 (40%), Gaps = 4/92 (4%) Frame = +2 Query: 167 IVVNLTLHLRHA---NIRESESTVRIQADLQMNXIDKRLSXNXGEWGCSTWL-VSSERLW 334 +VV L L L N+++ T + L+ D + + E+G T L V SE +W Sbjct: 46 VVVKLGLRLSQLIDLNLKDQILTTNVW--LEHEWQDHKFQWDPAEYGGVTELYVPSEHIW 103 Query: 335 MPDXVLLNXXXXTXGGLRSPXPRYLXTGSXSW 430 +PD VL N G TG W Sbjct: 104 LPDIVLYNNADGEYGVTTMTKAILHYTGKVLW 135 >DQ667183-1|ABG75735.1| 463|Apis mellifera GABA-gated ion channel protein. Length = 463 Score = 26.6 bits (56), Expect = 0.31 Identities = 14/63 (22%), Positives = 26/63 (41%) Frame = +2 Query: 206 IRESESTVRIQADLQMNXIDKRLSXNXGEWGCSTWLVSSERLWMPDXVLLNXXXXTXGGL 385 I E + T + + + +D+RL+ G+ + + R+W PD N + Sbjct: 44 ISEVDMTYSMDCYFRQSWVDRRLAFQGGKETLALSISMLARIWKPDTYFYNGKHSYLHTI 103 Query: 386 RSP 394 SP Sbjct: 104 TSP 106 >DQ667185-1|ABG75737.1| 447|Apis mellifera glutamate-gated chloride channel protein. Length = 447 Score = 26.2 bits (55), Expect = 0.42 Identities = 19/66 (28%), Positives = 30/66 (45%), Gaps = 3/66 (4%) Frame = +2 Query: 170 VVNLTLHLRH-ANIRESESTVRIQADLQMNXIDKRLSXNX--GEWGCSTWLVSSERLWMP 340 +V + L +R A I + + +Q + +D+RL N G T L + R+WMP Sbjct: 60 IVRVNLFVRSIATISDIKMEYSVQLTFREQWLDERLRFNDFGGRLKYLT-LTDASRVWMP 118 Query: 341 DXVLLN 358 D N Sbjct: 119 DLFFSN 124 >DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholine receptor beta1subunit protein. Length = 520 Score = 26.2 bits (55), Expect = 0.42 Identities = 16/67 (23%), Positives = 31/67 (46%), Gaps = 2/67 (2%) Frame = +2 Query: 164 KIVVNLTL-HLRHANIRESESTVRIQADLQMNXIDKRLSXNXGEWG-CSTWLVSSERLWM 337 K+ VN L ++ N+ E ++ L+ D +L + ++G + +++W Sbjct: 51 KVHVNFGLAFVQLINVNEKNQIMKSNVWLRFIWTDYQLQWDEADYGGIGVLRLPPDKVWK 110 Query: 338 PDXVLLN 358 PD VL N Sbjct: 111 PDIVLFN 117 >DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholine receptor alpha3subunit protein. Length = 566 Score = 25.8 bits (54), Expect = 0.55 Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 1/39 (2%) Frame = +2 Query: 245 LQMNXIDKRLSXNXGEWGCSTWL-VSSERLWMPDXVLLN 358 ++ + D +L + E+G L V S+ +W PD VL N Sbjct: 77 VEQSWYDYKLKWDPKEYGGVEMLHVPSDHIWRPDIVLYN 115 >DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 25.4 bits (53), Expect = 0.73 Identities = 18/67 (26%), Positives = 31/67 (46%), Gaps = 2/67 (2%) Frame = +2 Query: 164 KIVVNLTL-HLRHANIRESESTVRIQADLQMNXIDKRLSXNXGEWGCSTWL-VSSERLWM 337 ++ + L L L N++ T + ++ + D +L E+G L V S+ +W Sbjct: 55 RVCIKLKLSQLIDVNLKNQIMTTNLW--VEQSWYDYKLRWEPKEYGGVKMLHVPSDHIWR 112 Query: 338 PDXVLLN 358 PD VL N Sbjct: 113 PDIVLYN 119 >DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 25.4 bits (53), Expect = 0.73 Identities = 18/67 (26%), Positives = 31/67 (46%), Gaps = 2/67 (2%) Frame = +2 Query: 164 KIVVNLTL-HLRHANIRESESTVRIQADLQMNXIDKRLSXNXGEWGCSTWL-VSSERLWM 337 ++ + L L L N++ T + ++ + D +L E+G L V S+ +W Sbjct: 55 RVCIKLKLSQLIDVNLKNQIMTTNLW--VEQSWYDYKLRWEPKEYGGVKMLHVPSDHIWR 112 Query: 338 PDXVLLN 358 PD VL N Sbjct: 113 PDIVLYN 119 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 25.0 bits (52), Expect = 0.96 Identities = 17/65 (26%), Positives = 26/65 (40%), Gaps = 1/65 (1%) Frame = +2 Query: 167 IVVNLTLHLRHANIRESESTVRIQADLQMNXIDKRLSXNXGEW-GCSTWLVSSERLWMPD 343 +V L+LH ++ E + + D L N E+ G V R+W PD Sbjct: 33 VVFGLSLH-HIIDVDEKNQILTTNCWVTQIWTDHHLKWNASEFAGIRVIRVPYNRVWRPD 91 Query: 344 XVLLN 358 +L N Sbjct: 92 TILYN 96 >DQ667186-1|ABG75738.1| 447|Apis mellifera glutamate-gated chloride channel protein. Length = 447 Score = 23.4 bits (48), Expect = 2.9 Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 3/66 (4%) Frame = +2 Query: 170 VVNLTLHLRH-ANIRESESTVRIQADLQMNXIDKRLSXNX--GEWGCSTWLVSSERLWMP 340 VV + + +R + I + +Q + +D+RL N G T L + R+WMP Sbjct: 60 VVRVNIFVRSISKIDDVTMEYSVQLTFREQWLDERLRFNDFGGRLKYLT-LTDASRVWMP 118 Query: 341 DXVLLN 358 D N Sbjct: 119 DLFFSN 124 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 143,712 Number of Sequences: 438 Number of extensions: 2586 Number of successful extensions: 11 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 11 length of database: 146,343 effective HSP length: 58 effective length of database: 120,939 effective search space used: 29750994 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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