Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_FL5_L11
(889 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 233 9e-62
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 54 1e-07
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 54 1e-07
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 51 1e-06
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 50 3e-06
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 33 0.33
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 33 0.33
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 31 1.4
At5g06970.1 68418.m00789 expressed protein 30 1.8
At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas... 30 2.4
At5g67240.1 68418.m08475 exonuclease family protein contains exo... 29 4.1
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 29 5.4
At5g40740.1 68418.m04944 expressed protein 28 9.5
At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N domain-... 28 9.5
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 233 bits (571), Expect = 9e-62
Identities = 117/210 (55%), Positives = 145/210 (69%), Gaps = 3/210 (1%)
Frame = +2
Query: 74 SLESIINNNLTGRDLEEFNRIHFGR---RNNLEIKLKESSIXXXXXXXXXXXXXXFPAKD 244
SL +++ NL +E NR+ GR R+ +I L ES+ F A
Sbjct: 22 SLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAASFSADK 81
Query: 245 EQTRPPRIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNM 424
EQ R PR+V+VG+IQ+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W M
Sbjct: 82 EQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTM 141
Query: 425 PFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVV 604
PFAFCTRE++ WCEFAE DG +T FL+ELA KY MVIVS ILERD H ++LWNTAV+
Sbjct: 142 PFAFCTRERR-WCEFAEPV-DGESTKFLQELAKKYNMVIVSPILERDIDHGEVLWNTAVI 199
Query: 605 ISDTGNVIGKHRKNHIPRVGDFNESNYYME 694
I + GN+IGKHRKNHIPRVGDFNES YYME
Sbjct: 200 IGNNGNIIGKHRKNHIPRVGDFNESTYYME 229
Score = 77.8 bits (183), Expect = 9e-15
Identities = 36/63 (57%), Positives = 42/63 (66%)
Frame = +3
Query: 693 KGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGXNGAEIVLIRXRXSPEKGXQLXWNXE 872
+G+TGHPVF T +GKIAVNIC+GRHH LNW+ FG NGAEIV E + W E
Sbjct: 229 EGDTGHPVFETVFGKIAVNICYGRHHPLNWLAFGLNGAEIVFNPSATVGELSEPM-WPIE 287
Query: 873 XRN 881
RN
Sbjct: 288 ARN 290
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 54.0 bits (124), Expect = 1e-07
Identities = 32/113 (28%), Positives = 55/113 (48%), Gaps = 2/113 (1%)
Frame = +2
Query: 320 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 493
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 494 TTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHI 652
+T L E++ + + I+ + E+ D L+NT V G + KHRK H+
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHL 204
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 54.0 bits (124), Expect = 1e-07
Identities = 32/113 (28%), Positives = 55/113 (48%), Gaps = 2/113 (1%)
Frame = +2
Query: 320 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 493
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 494 TTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHI 652
+T L E++ + + I+ + E+ D L+NT V G + KHRK H+
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHL 204
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 50.8 bits (116), Expect = 1e-06
Identities = 32/112 (28%), Positives = 61/112 (54%), Gaps = 1/112 (0%)
Frame = +2
Query: 356 KKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAM 535
++++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA + +
Sbjct: 31 ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89
Query: 536 VI-VSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYY 688
VI VS E + H +N+ +I G +G +RK+HIP + E Y+
Sbjct: 90 VIPVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYF 137
Score = 28.7 bits (61), Expect = 5.4
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +3
Query: 696 GNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGXNGAEIV 815
G+TG VF T++ KI V IC+ + GAEI+
Sbjct: 140 GDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQGAEIL 179
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 49.6 bits (113), Expect = 3e-06
Identities = 32/110 (29%), Positives = 59/110 (53%), Gaps = 1/110 (0%)
Frame = +2
Query: 362 IIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVI 541
++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA + +VI
Sbjct: 60 LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118
Query: 542 -VSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYY 688
VS E + H +N+ +I G +G +RK+HIP + E Y+
Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYF 164
Score = 28.7 bits (61), Expect = 5.4
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +3
Query: 696 GNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGXNGAEIV 815
G+TG VF T++ KI V IC+ + GAEI+
Sbjct: 167 GDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVLQGAEIL 206
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 32.7 bits (71), Expect = 0.33
Identities = 42/142 (29%), Positives = 59/142 (41%), Gaps = 13/142 (9%)
Frame = +2
Query: 257 PPRIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELW------ 418
P V+V I+Q S V D P A +K +K I A +G ++ F E +
Sbjct: 21 PSSTVRVTIVQSS-TVYNDTP------ATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPR 73
Query: 419 --NMPFAFCTREKQPWCEFAE---SA--EDGPTTTFLRELAIKYAMVIVSSILERDEKHS 577
A ++ EF SA GP L ELA K + +V +E+D
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKD---G 130
Query: 578 DILWNTAVVISDTGNVIGKHRK 643
L+ TA+ S G +GKHRK
Sbjct: 131 YTLYCTALFFSPQGQFLGKHRK 152
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 32.7 bits (71), Expect = 0.33
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = +2
Query: 488 GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 643
GP L ELA K + +V +E+D L+ TA+ S G +GKHRK
Sbjct: 97 GPEVEKLAELAGKNNVYLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRK 145
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 30.7 bits (66), Expect = 1.4
Identities = 29/115 (25%), Positives = 51/115 (44%), Gaps = 13/115 (11%)
Frame = +2
Query: 338 AIFNKVKKIIDVAGQEGVNIICFQELW--------NMPFAFCTREKQPWCEFAE---SAE 484
A +K ++++ A + G ++ F E + A +R + +F + SA
Sbjct: 52 ATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAI 111
Query: 485 D--GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 643
D GP L +A KY + +V ++ER+ L+ T + G +GKHRK
Sbjct: 112 DVPGPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHRK 163
>At5g06970.1 68418.m00789 expressed protein
Length = 1101
Score = 30.3 bits (65), Expect = 1.8
Identities = 14/59 (23%), Positives = 27/59 (45%)
Frame = -2
Query: 639 RCFPITFPVSLMTTAVFQSMSECFSSLSNIEDTITIAYLMASSRRKVVVGPSSADSANS 463
R I P + M ++ S LS +ED++ + ++ R K+V+ S + + S
Sbjct: 834 RSINIDVPATAMLCVQLNTLHYAVSQLSKLEDSMWLRWIAKKPREKIVIRKSMVEKSKS 892
>At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1
[Mus musculus] GI:3228668; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 307
Score = 29.9 bits (64), Expect = 2.4
Identities = 30/105 (28%), Positives = 46/105 (43%), Gaps = 2/105 (1%)
Frame = +2
Query: 344 FNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAI 523
F +++ A G +ICF E F+F ++ + AE DGP LA
Sbjct: 54 FATCSRLVQEAALAGAKLICFPE----NFSFVGDKEGESVKIAEPL-DGPVMERYCSLAR 108
Query: 524 KYAMVI-VSSILER-DEKHSDILWNTAVVISDTGNVIGKHRKNHI 652
+ + + ER D+ H L NT VVI D G + ++K H+
Sbjct: 109 DSNIWLSLGGFQERFDDTH---LCNTHVVIDDAGMIRDTYQKMHL 150
>At5g67240.1 68418.m08475 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 745
Score = 29.1 bits (62), Expect = 4.1
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 496 HDLPSGTRHQVRNGDRVLDIRKGREAFGH 582
HD+PS H V NGD LD++ + G+
Sbjct: 441 HDVPSQELHGVLNGDFTLDVKPPKRKGGY 469
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 28.7 bits (61), Expect = 5.4
Identities = 38/142 (26%), Positives = 58/142 (40%), Gaps = 13/142 (9%)
Frame = +2
Query: 257 PPRIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQE--LWNMPF 430
P V+V I+Q S V D P A +K +K I A +G ++ F E + P
Sbjct: 21 PSTTVRVTIVQSS-TVYNDTP------ATIDKAEKYIVEAASKGAELVLFPEGFIGGYPR 73
Query: 431 AFC---------TREKQPWCEFAESA--EDGPTTTFLRELAIKYAMVIVSSILERDEKHS 577
F + + ++ SA GP L ++A K + +V +E K
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIE---KEG 130
Query: 578 DILWNTAVVISDTGNVIGKHRK 643
L+ T + S G +GKHRK
Sbjct: 131 YTLYCTVLFFSPQGQFLGKHRK 152
>At5g40740.1 68418.m04944 expressed protein
Length = 741
Score = 27.9 bits (59), Expect = 9.5
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +2
Query: 557 ERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESN 682
ER+ + ++ +++ NVIG N PRVG F SN
Sbjct: 8 ERELELESAMYTNCLLLGLDPNVIGLGASNGTPRVGLFRHSN 49
>At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N
domain-containing protein contains Pfam profile PF00627:
UBA/TS-N domain
Length = 649
Score = 27.9 bits (59), Expect = 9.5
Identities = 17/38 (44%), Positives = 25/38 (65%), Gaps = 2/38 (5%)
Frame = -2
Query: 615 VSLMTTAVFQSMSECFSSLSNIEDT-ITIAYL-MASSR 508
+ L + + FQ+ FSSL+NI+ T T+A L MASS+
Sbjct: 482 IGLPSQSQFQASGSSFSSLTNIDPTDFTVANLPMASSQ 519
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,462,890
Number of Sequences: 28952
Number of extensions: 392502
Number of successful extensions: 1146
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1090
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1141
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2081245872
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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