Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BmNP01_FL5_L08
         (830 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.10c |||EPS15 repeat family actin cortical patch componen...    94   2e-20
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo...    60   3e-10
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    57   3e-09
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha...    47   3e-06
SPCC645.13 |||transcription elongation regulator|Schizosaccharom...    29   0.61 
SPAC11E3.06 |map1||MADS-box transcription factor Map1|Schizosacc...    27   2.5  
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|...    27   4.3  
SPAC1F12.09 |gpi17||pig-S|Schizosaccharomyces pombe|chr 1|||Manual     26   5.7  
SPAC1F7.09c |||allantoicase |Schizosaccharomyces pombe|chr 1|||M...    26   7.5  
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe...    26   7.5  
SPAC29E6.02 |prp3|SPAC30.06|U4/U6 x U5 tri-snRNP complex subunit...    26   7.5  
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    25   10.0 
SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Sch...    25   10.0 
SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr...    25   10.0 

>SPBC800.10c |||EPS15 repeat family actin cortical patch component
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1116

 Score = 94.3 bits (224), Expect = 2e-20
 Identities = 43/99 (43%), Positives = 68/99 (68%), Gaps = 1/99 (1%)
 Frame = +3

Query: 129 MADPWTIQAHEHAKFSEHFRNLGPVN-GNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNA 305
           ++DPW I + +   F + F N+   + G ++G +A  F L S+LP  VL QIW L+DTN+
Sbjct: 249 VSDPWAIPSQDLTSFCQLFSNVDKAHKGYVSGSEAYSFFLASKLPEDVLAQIWDLSDTNS 308

Query: 306 DGKLDLKEFSIACKIINLKLRGLEVPKMLPPSLIASLSP 422
           +GKL++ EF I+  +I LKL G E+PK+LP S+++S++P
Sbjct: 309 NGKLNIGEFCISLYLIKLKLSGKELPKVLPSSMLSSVAP 347



 Score = 61.7 bits (143), Expect = 1e-10
 Identities = 32/95 (33%), Positives = 55/95 (57%), Gaps = 2/95 (2%)
 Frame = +3

Query: 147 IQAHEHAKFSEHFRNLGPVNGNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADGKLDLK 326
           I + E  ++ + F  + P +G + G++A     ++ L   +L ++W+L DT+  G LD++
Sbjct: 130 ISSDEMTRYQQMFTTVCPTDGLMDGDRASSIFGRAPLSTEILARVWNLVDTHKRGALDIR 189

Query: 327 EFSIACKIINLKLRG-LEVPKM-LPPSLIASLSPT 425
           EF+    IINL L G L+ P + + PS IAS + T
Sbjct: 190 EFNTGMHIINLLLNGSLKSPPVSISPSFIASAAST 224



 Score = 48.8 bits (111), Expect = 9e-07
 Identities = 27/72 (37%), Positives = 39/72 (54%), Gaps = 1/72 (1%)
 Frame = +3

Query: 147 IQAHEHAKFSEHFRNLGPVN-GNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADGKLDL 323
           + A E   F + F+     + G +TGE+A  F+ +S L P VLGQIW +AD    G L  
Sbjct: 5   LSAEEQTAFDQLFKIADKQDIGVITGEEAVPFLEKSGLAPQVLGQIWQIADAENRGFLTF 64

Query: 324 KEFSIACKIINL 359
             F IA +++ L
Sbjct: 65  SGFVIAMRLVAL 76


>SPBC11G11.02c |end3||actin cortical patch component End3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 375

 Score = 60.5 bits (140), Expect = 3e-10
 Identities = 34/87 (39%), Positives = 49/87 (56%), Gaps = 2/87 (2%)
 Frame = +3

Query: 159 EHAKFSEHFRNLGPVNGNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADGKLDLKEFSI 338
           E  K+ E FR L P NG L+G +A   +  S+L    L +IW LAD + DG  D  EF+I
Sbjct: 5   EKNKYWEIFRGLNPENGYLSGSKAAGVLRSSKLSSDKLEKIWDLADIDDDGMFDFDEFAI 64

Query: 339 ACKIINLKLRGL--EVPKMLPPSLIAS 413
           A KI    + G+   VP  +P +L+++
Sbjct: 65  AMKITFDLINGVYKTVPDRVPEALVST 91


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 57.2 bits (132), Expect = 3e-09
 Identities = 34/91 (37%), Positives = 42/91 (46%), Gaps = 1/91 (1%)
 Frame = +3

Query: 138  PWTIQAHEHAKFSEHFRNLGPVN-GNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADGK 314
            PW I   E   + + F        G L G        QS+L    L  IW+L D    G 
Sbjct: 814  PWAISKEEKRIYDQIFDAWDKERKGTLGGNAVLEIFGQSKLTRTELEHIWNLCDHGDKGS 873

Query: 315  LDLKEFSIACKIINLKLRGLEVPKMLPPSLI 407
            LD  EF++A  +I  KL G EVP +LPP LI
Sbjct: 874  LDRDEFAVALHLIYRKLNGNEVPAVLPPELI 904



 Score = 54.4 bits (125), Expect = 2e-08
 Identities = 26/87 (29%), Positives = 47/87 (54%)
 Frame = +3

Query: 147 IQAHEHAKFSEHFRNLGPVNGNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADGKLDLK 326
           + A + AKF + F++       ++ E  K  +++S+LP   L +IW L+DT   G+L   
Sbjct: 277 VSAADQAKFEQLFKSAVGREEAMSSEIGKAILVRSKLPTVQLSKIWRLSDTTRSGRLLFP 336

Query: 327 EFSIACKIINLKLRGLEVPKMLPPSLI 407
           +F +A  + NL L G  +P  +P  ++
Sbjct: 337 QFVLAMYLCNLGLTGKPIPDKVPDGIL 363


>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
           Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 884

 Score = 47.2 bits (107), Expect = 3e-06
 Identities = 26/83 (31%), Positives = 41/83 (49%), Gaps = 1/83 (1%)
 Frame = +3

Query: 147 IQAHEHAKFSEHFRNLGPVN-GNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADGKLDL 323
           I   + + F + F  +   N G + G +A  F + S L    L +IW   DT   G +D 
Sbjct: 296 ITPEDRSNFYQLFSKIDNENKGYIVGGEAVPFFMASHLDSEELARIWDTVDTQDRGYIDK 355

Query: 324 KEFSIACKIINLKLRGLEVPKML 392
            EF++A +II L+L G  +  +L
Sbjct: 356 DEFAVAMEIIKLRLSGKSLASIL 378



 Score = 40.3 bits (90), Expect = 3e-04
 Identities = 34/135 (25%), Positives = 63/135 (46%), Gaps = 4/135 (2%)
 Frame = +3

Query: 207 GNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADGKLDLKEFSIACKIINL--KLRGLEV 380
           G +    AK  +L+S L   VL QIW+LAD+   G L++ ++ IA  ++ +  +   + +
Sbjct: 151 GCMPSSIAKPILLKSSLHYAVLAQIWNLADSAHVGYLEMYQYVIARHLVAICKQYNLIHL 210

Query: 381 PKMLPPSLIASL-SPT-GRGPNLLRGSRA*GCAVCVRLNDLQVLRAVRGPKDFRAHPLND 554
           P+ LP  +I S  S T     N+ +G          +L+  Q + + +  K       N+
Sbjct: 211 PRSLPADVIESAKSETPAMNTNIEQGVTQPSLVNTEQLSHQQSISSTKSSKLDEISADNN 270

Query: 555 SPGTLTPDVRSPPRS 599
           +   +     SPP++
Sbjct: 271 AQSAVDNKYSSPPQT 285



 Score = 34.7 bits (76), Expect = 0.016
 Identities = 21/85 (24%), Positives = 36/85 (42%), Gaps = 1/85 (1%)
 Frame = +3

Query: 138 PWTIQAHEHAKFSEHFRNLGPVNGNLT-GEQAKRFMLQSQLPPPVLGQIWSLADTNADGK 314
           P  +   E  +F     +L P   +L  G     F+L+  LP  +L +IW   D    G 
Sbjct: 3   PLQLSPIEQQEFDRLLESLHPEQKDLIPGSVIGPFLLKFGLPQKILAKIWDYCDQEDKGS 62

Query: 315 LDLKEFSIACKIINLKLRGLEVPKM 389
           LD  +     ++I+   RG  + ++
Sbjct: 63  LDRNQVYACFRLISQAQRGATLQEL 87


>SPCC645.13 |||transcription elongation
           regulator|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 721

 Score = 29.5 bits (63), Expect = 0.61
 Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%)
 Frame = +3

Query: 561 GTLTP-DVRSPPRSEPR*GKGVTAVNTTTRQRGSPQGRPADGAFET 695
           G+ TP    SPP +  R G   T    T R + +P+  P D + ET
Sbjct: 152 GSTTPKSSHSPPSTRKRRGSVGTTATHTKRSKNAPKTSPKDASNET 197


>SPAC11E3.06 |map1||MADS-box transcription factor
           Map1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 398

 Score = 27.5 bits (58), Expect = 2.5
 Identities = 17/50 (34%), Positives = 24/50 (48%)
 Frame = +2

Query: 482 ETQRSAGVESSSRSQGF*GPPTKRLPWHSYTRRAIPSEVRTQMR*GGYRG 631
           +T R   +  S +S     PP  R P    +R A+PS + T+ R   YRG
Sbjct: 292 QTPRKNKIRDSLQSSPLNFPPRDRPPLIPISRIAVPSTIETEER--QYRG 339


>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1184

 Score = 26.6 bits (56), Expect = 4.3
 Identities = 20/49 (40%), Positives = 23/49 (46%)
 Frame = +1

Query: 514 FAVPRILGPTH*TTPLALLHPTCDPLRGQNPDEVRGLPRSTLQPDSAAH 660
           FA P  LGP   T+  A    T  P  G  PDE R +  S L   SA+H
Sbjct: 160 FAFP--LGPVFATSSQAAASETALP-SGYEPDEARFIGASQLAKLSASH 205


>SPAC1F12.09 |gpi17||pig-S|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 554

 Score = 26.2 bits (55), Expect = 5.7
 Identities = 22/72 (30%), Positives = 33/72 (45%)
 Frame = +3

Query: 42  SFFRRISLKSILLGQTTAKVQRNVFNSEMMADPWTIQAHEHAKFSEHFRNLGPVNGNLTG 221
           SF+  I L   +  +TT   + ++   +M   P T+Q H   +FS  FR L     NLT 
Sbjct: 78  SFYVIILLAIPVWWKTTHYERSSLPFEDMENAPSTVQTH--LRFSPTFRILDDKGNNLTK 135

Query: 222 EQAKRFMLQSQL 257
           E  K    + Q+
Sbjct: 136 EVQKVLEAEPQI 147


>SPAC1F7.09c |||allantoicase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 342

 Score = 25.8 bits (54), Expect = 7.5
 Identities = 16/60 (26%), Positives = 22/60 (36%)
 Frame = +3

Query: 135 DPWTIQAHEHAKFSEHFRNLGPVNGNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADGK 314
           D W  + H  A        LGP +G +TG +           P V  +   L + N D K
Sbjct: 69  DGWETRRHNTAPCDWVIVKLGPSSGRVTGCEIDTTFFNGNHAPEVSVEAAFLPEGNPDAK 128


>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 551

 Score = 25.8 bits (54), Expect = 7.5
 Identities = 15/42 (35%), Positives = 18/42 (42%)
 Frame = +2

Query: 482 ETQRSAGVESSSRSQGF*GPPTKRLPWHSYTRRAIPSEVRTQ 607
           E   S G  SSSR  G    PT   PW+      +  EV T+
Sbjct: 437 ELSASPGSVSSSRHSGIFATPTFLSPWNIKNIPLVTPEVSTR 478


>SPAC29E6.02 |prp3|SPAC30.06|U4/U6 x U5 tri-snRNP complex subunit
           Prp3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 542

 Score = 25.8 bits (54), Expect = 7.5
 Identities = 15/43 (34%), Positives = 22/43 (51%), Gaps = 2/43 (4%)
 Frame = +2

Query: 584 IPSEVRTQMR*GGYRGQHYNQTARLTPRTPSRRSFR--DESKA 706
           I +EVR Q+     R +  N+  +LTP     ++FR  DE  A
Sbjct: 353 IEAEVRKQVEERRLRHERENEERKLTPEERKEKAFRKKDEDSA 395


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 25.4 bits (53), Expect = 10.0
 Identities = 10/25 (40%), Positives = 17/25 (68%)
 Frame = +3

Query: 258 PPPVLGQIWSLADTNADGKLDLKEF 332
           P   + +I+++ D N DG+L L+EF
Sbjct: 145 PEKRVNKIFNMMDKNKDGQLTLEEF 169


>SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase
           Ubp14|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 775

 Score = 25.4 bits (53), Expect = 10.0
 Identities = 14/42 (33%), Positives = 21/42 (50%)
 Frame = -2

Query: 436 GPRPVGLKEAIRDGGSIFGTSKPRSFKFIILQAIENSFKSSL 311
           G RP   K+ +  G S FGTS+ +     +L  +    KSS+
Sbjct: 384 GLRPFMFKDVVGQGHSEFGTSQQQDAYEFLLYLLGKIRKSSI 425


>SPBP35G2.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1060

 Score = 25.4 bits (53), Expect = 10.0
 Identities = 10/30 (33%), Positives = 16/30 (53%)
 Frame = -1

Query: 539 GPKILGTANCSQHLQIVESHTHRAPLCAGT 450
           GP++   ++  QHL  +  HTH  P  + T
Sbjct: 160 GPRMSSVSSGKQHLSSLSLHTHFNPSSSST 189


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,263,798
Number of Sequences: 5004
Number of extensions: 69109
Number of successful extensions: 210
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)