Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_FL5_L08
(830 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g21630.1 68414.m02708 calcium-binding EF hand family protein ... 56 4e-08
At3g20290.1 68416.m02571 calcium-binding EF hand family protein ... 55 7e-08
At1g20760.1 68414.m02600 calcium-binding EF hand family protein ... 55 7e-08
At4g12750.1 68417.m02002 expressed protein 29 2.9
At5g41500.1 68418.m05039 F-box family protein contains Pfam PF00... 29 3.8
At1g56130.1 68414.m06445 leucine-rich repeat family protein / pr... 29 3.8
At1g76650.1 68414.m08919 calcium-binding EF hand family protein ... 29 5.0
At5g18700.1 68418.m02219 protein kinase-related contains protein... 28 6.6
At4g03560.1 68417.m00488 two-pore calcium channel (TPC1) identic... 28 6.6
At3g50360.1 68416.m05507 caltractin / centrin identical to caltr... 28 6.6
At2g38410.1 68415.m04718 VHS domain-containing protein / GAT dom... 28 6.6
At2g36180.1 68415.m04440 calmodulin-related protein, putative si... 28 6.6
At5g57190.1 68418.m07144 phosphatidylserine decarboxylase, putat... 28 8.7
At4g26540.1 68417.m03823 protein kinase family protein Three fal... 28 8.7
At3g19890.1 68416.m02518 F-box family protein contains F-box dom... 28 8.7
At1g56140.1 68414.m06446 leucine-rich repeat family protein / pr... 28 8.7
>At1g21630.1 68414.m02708 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain; ESTs gb|T44428 and gb|AA395440 come from this
gene
Length = 1218
Score = 55.6 bits (128), Expect = 4e-08
Identities = 27/72 (37%), Positives = 40/72 (55%)
Frame = +3
Query: 204 NGNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADGKLDLKEFSIACKIINLKLRGLEVP 383
+G +TG QA+ L +LP L Q+W L+D + D L L+EF IA ++ G +P
Sbjct: 440 DGKITGNQARNLFLSWRLPRDALKQVWDLSDQDNDSMLSLREFCIAVYLMERYREGRPLP 499
Query: 384 KMLPPSLIASLS 419
+ P S+I S S
Sbjct: 500 PVFPSSIIHSES 511
>At3g20290.1 68416.m02571 calcium-binding EF hand family protein
similar to EH-domain containing protein 1 from {Mus
musculus} SP|Q9WVK4 and {Homo sapiens} SP|Q9H4M9,
receptor-mediated endocytosis 1 from [Caenorhabditis
elegans] GI:13487775, GI:13487777, GI:13487779; contains
INTERPRO:IPR002048 calcium-binding EF-hand domain
Length = 545
Score = 54.8 bits (126), Expect = 7e-08
Identities = 24/59 (40%), Positives = 36/59 (61%)
Frame = +3
Query: 204 NGNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADGKLDLKEFSIACKIINLKLRGLEV 380
+G +TG A +F S LP P L QIW++AD+ G L KEF +A ++++L G E+
Sbjct: 31 DGRITGNDAIKFFTMSNLPRPELKQIWAIADSKRQGYLGFKEFIVAMQLVSLAQTGHEI 89
>At1g20760.1 68414.m02600 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 1019
Score = 54.8 bits (126), Expect = 7e-08
Identities = 29/92 (31%), Positives = 50/92 (54%), Gaps = 2/92 (2%)
Frame = +3
Query: 138 PWT-IQAHEHAKFSEHFRNL-GPVNGNLTGEQAKRFMLQSQLPPPVLGQIWSLADTNADG 311
PW ++ + K+++ F + +G +TGEQA+ L +LP VL +W L+D + D
Sbjct: 351 PWPKMKPSDVQKYTKVFMEVDSDKDGKITGEQARNLFLSWRLPREVLKHVWELSDQDNDT 410
Query: 312 KLDLKEFSIACKIINLKLRGLEVPKMLPPSLI 407
L L+EF I+ ++ G +P LP S++
Sbjct: 411 MLSLREFCISLYLMERYREGRPLPTALPSSIM 442
>At4g12750.1 68417.m02002 expressed protein
Length = 1108
Score = 29.5 bits (63), Expect = 2.9
Identities = 14/27 (51%), Positives = 17/27 (62%)
Frame = -1
Query: 632 DRGNPLTSSGF*PRRGSHVGCKSARGV 552
+RGNPLT S P GSH GC +G+
Sbjct: 276 ERGNPLTCSCHHPSSGSH-GCFLCKGI 301
>At5g41500.1 68418.m05039 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 403
Score = 29.1 bits (62), Expect = 3.8
Identities = 17/44 (38%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = -1
Query: 365 EFQVYYLASYRELLQIQLTVGVR-VRQ*PYLSQDRWRKLRL*HK 237
EF +Y + Y LL L R V PYL Q+RW ++R H+
Sbjct: 95 EFFIYRVIHYEGLLLCILKDATRIVVWNPYLGQERWIQIRYSHR 138
>At1g56130.1 68414.m06445 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 1032
Score = 29.1 bits (62), Expect = 3.8
Identities = 15/43 (34%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
Frame = +3
Query: 333 SIACKIINLKLRGLEVPKMLPPSL--IASLSPTGRGPNLLRGS 455
S C+I N+K+ ++V +PP L + L+ G N+L GS
Sbjct: 96 STICRITNIKVYAIDVVGPIPPELWTLTYLTNLNLGQNVLTGS 138
>At1g76650.1 68414.m08919 calcium-binding EF hand family protein
similar to regulator of gene silencing
calmodulin-related protein GI:12963415 from [Nicotiana
tabacum]; contains INTERPRO:IPR002048 calcium-binding
EF-hand domain
Length = 177
Score = 28.7 bits (61), Expect = 5.0
Identities = 20/61 (32%), Positives = 33/61 (54%), Gaps = 2/61 (3%)
Frame = +3
Query: 204 NGNLTGEQA-KRFM-LQSQLPPPVLGQIWSLADTNADGKLDLKEFSIACKIINLKLRGLE 377
+G ++ E+ K FM L QL L+DT+ DG LD +EFS K+ + + + +E
Sbjct: 56 DGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLIKVDDEEEKKME 115
Query: 378 V 380
+
Sbjct: 116 L 116
>At5g18700.1 68418.m02219 protein kinase-related contains protein
kinase domain, INTERPRO:IPR000719
Length = 1366
Score = 28.3 bits (60), Expect = 6.6
Identities = 15/35 (42%), Positives = 24/35 (68%)
Frame = -1
Query: 239 KSFRLFARQISIDRSKVSKVLRELRMLVCLYSPRV 135
K+ FA + S+D+S+ +KVL+E+R+L L P V
Sbjct: 25 KTIEYFACK-SVDKSRKNKVLQEVRILHSLNHPNV 58
>At4g03560.1 68417.m00488 two-pore calcium channel (TPC1) identical
to two-pore calcium channel (TPC1) [Arabidopsis
thaliana] gi|14041819|dbj|BAB55460
Length = 733
Score = 28.3 bits (60), Expect = 6.6
Identities = 15/56 (26%), Positives = 26/56 (46%)
Frame = +3
Query: 222 EQAKRFMLQSQLPPPVLGQIWSLADTNADGKLDLKEFSIACKIINLKLRGLEVPKM 389
EQ + ++ G I+ D D K++ EF+ C+ I L+ + EVP +
Sbjct: 352 EQLTNYRTLPKISKEEFGLIFDELDDTRDFKINKDEFADLCQAIALRFQKEEVPSL 407
>At3g50360.1 68416.m05507 caltractin / centrin identical to
caltractin; centrin GI:3688162 from [Arabidopsis
thaliana]
Length = 169
Score = 28.3 bits (60), Expect = 6.6
Identities = 10/39 (25%), Positives = 23/39 (58%)
Frame = +3
Query: 276 QIWSLADTNADGKLDLKEFSIACKIINLKLRGLEVPKML 392
+ + L DT+ G +D KE ++A + + ++ ++ KM+
Sbjct: 30 EAFELFDTDGSGTIDAKELNVAMRALGFEMTEEQINKMI 68
>At2g38410.1 68415.m04718 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 671
Score = 28.3 bits (60), Expect = 6.6
Identities = 24/79 (30%), Positives = 32/79 (40%), Gaps = 1/79 (1%)
Frame = +3
Query: 552 DSPGTLTPDVRSPPRSEPR*GKGVTAVNTTTRQRGSPQGRPADGAFETNRRL*NVXRLGT 731
D+ +TP V PP +P+ G GV Q G G P G + + G
Sbjct: 149 DASPIITPPVSHPPLRQPQGGYGVPPAGYGVHQAG--YGVPQAGY--------GIPQAGY 198
Query: 732 AVRQAAXTVPXVS-GIPXG 785
V QA +P V G+P G
Sbjct: 199 GVPQAGYGIPQVGYGMPSG 217
>At2g36180.1 68415.m04440 calmodulin-related protein, putative
similar to calmodulin-related protein 2, touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 144
Score = 28.3 bits (60), Expect = 6.6
Identities = 12/40 (30%), Positives = 23/40 (57%)
Frame = +3
Query: 270 LGQIWSLADTNADGKLDLKEFSIACKIINLKLRGLEVPKM 389
+ +I+ D N DGK+ EF+ A ++ + ++ E+ KM
Sbjct: 1 MAEIFESVDKNKDGKILWDEFAEAIRVFSPQITSEEIDKM 40
>At5g57190.1 68418.m07144 phosphatidylserine decarboxylase, putative
similar to SP|P53037 Phosphatidylserine decarboxylase
proenzyme 2 precursor (EC 4.1.1.65) {Saccharomyces
cerevisiae}; contains Pfam profile PF02666:
phosphatidylserine decarboxylase
Length = 615
Score = 27.9 bits (59), Expect = 8.7
Identities = 21/85 (24%), Positives = 33/85 (38%), Gaps = 2/85 (2%)
Frame = +3
Query: 87 TTAKVQRNVFNSEMMADPWTIQAHEHAKFSEHFRNLGPVNGNLTGEQAKRFMLQSQLPPP 266
+T +V RN D + E + F L P + N+ G ++ +
Sbjct: 96 STNRVARNKIIGYCELDIFDFVVQEPESTCKSFNLLDPTSSNVVGSIFLSCAIEDPVETE 155
Query: 267 --VLGQIWSLADTNADGKLDLKEFS 335
+I S+ D N DG+L EFS
Sbjct: 156 RRFAKRILSIVDYNEDGQLSFSEFS 180
>At4g26540.1 68417.m03823 protein kinase family protein Three false
introns were added with non-consensus splice sites to
circumenvent frameshifts likely due to sequencing
errors; this is extremely unusual and is under
investigation.
Length = 1089
Score = 27.9 bits (59), Expect = 8.7
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = -2
Query: 394 GSIFGTSKPRSFKFIILQAIENSFKSSLP 308
GS+ GT+ P+S KFI +N+ S+LP
Sbjct: 514 GSLLGTTLPKSLKFIDFS--DNALSSTLP 540
>At3g19890.1 68416.m02518 F-box family protein contains F-box domain
Pfam:PF00646
Length = 410
Score = 27.9 bits (59), Expect = 8.7
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = -1
Query: 284 PYLSQDRWRKLRL*HKSFRLFARQISIDRSKVSKVLR 174
PYL Q +W + R H +FA + D+ K K+LR
Sbjct: 122 PYLGQTKWIEAREPHDESDMFA--LGYDKDKNHKILR 156
>At1g56140.1 68414.m06446 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 1032
Score = 27.9 bits (59), Expect = 8.7
Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = +3
Query: 327 EFSIACKIINLKLRGLEVPKMLPPSL--IASLSPTGRGPNLLRGS 455
E S C+I N+K+ +EV +P L + L+ G N+L GS
Sbjct: 93 ENSTICRITNIKVYAMEVVGSIPQQLWTLEYLTNLNLGQNVLTGS 137
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,008,244
Number of Sequences: 28952
Number of extensions: 405361
Number of successful extensions: 1205
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1204
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1911862400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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