Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_FL5_J04
(874 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 293 8e-80
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 71 8e-13
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 70 2e-12
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 60 2e-09
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 60 2e-09
At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas... 33 0.19
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 33 0.25
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 32 0.44
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 32 0.44
At5g06970.1 68418.m00789 expressed protein 30 1.8
At5g67240.1 68418.m08475 exonuclease family protein contains exo... 29 4.1
At2g44760.1 68415.m05571 expressed protein 29 5.4
At5g45720.1 68418.m05621 hypothetical protein 28 7.1
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 28 7.1
At5g40740.1 68418.m04944 expressed protein 28 9.4
At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N domain-... 28 9.4
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 293 bits (720), Expect = 8e-80
Identities = 143/250 (57%), Positives = 176/250 (70%), Gaps = 3/250 (1%)
Frame = +1
Query: 73 SLESIINNNLTGRDLEEFNRIHFGR---RNNLEIKLKESSIXXXXXXXXXXXXXXFPAKD 243
SL +++ NL +E NR+ GR R+ +I L ES+ F A
Sbjct: 22 SLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAASFSADK 81
Query: 244 EQTRPPRIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNM 423
EQ R PR+V+VG+IQ+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W M
Sbjct: 82 EQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTM 141
Query: 424 PFAFCTREKQPWCEFAESAEDGPTTTFLRELAIRYAMVIVSSILERDEKHSDILWNTAVV 603
PFAFCTRE++ WCEFAE DG +T FL+ELA +Y MVIVS ILERD H ++LWNTAV+
Sbjct: 142 PFAFCTRERR-WCEFAEPV-DGESTKFLQELAKKYNMVIVSPILERDIDHGEVLWNTAVI 199
Query: 604 ISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFAXRYGKIAVXICFGRDHVLNWM 783
I + GN+IGKHRKNHIPRVGDFNES YYMEG+TGHPVF +GKIAV IC+GR H LNW+
Sbjct: 200 IGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETVFGKIAVNICYGRHHPLNWL 259
Query: 784 MFGXNGXEIV 813
FG NG EIV
Sbjct: 260 AFGLNGAEIV 269
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 71.3 bits (167), Expect = 8e-13
Identities = 42/135 (31%), Positives = 74/135 (54%), Gaps = 1/135 (0%)
Frame = +1
Query: 355 KKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIRYAM 534
++++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA +
Sbjct: 31 ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89
Query: 535 VI-VSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHP 711
VI VS E + H +N+ +I G +G +RK+HIP + E Y+ G+TG
Sbjct: 90 VIPVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFK 145
Query: 712 VFAXRYGKIAVXICF 756
VF ++ KI V IC+
Sbjct: 146 VFQTKFAKIGVAICW 160
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 70.1 bits (164), Expect = 2e-12
Identities = 42/133 (31%), Positives = 72/133 (54%), Gaps = 1/133 (0%)
Frame = +1
Query: 361 IIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIRYAMVI 540
++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA +VI
Sbjct: 60 LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118
Query: 541 -VSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVF 717
VS E + H +N+ +I G +G +RK+HIP + E Y+ G+TG VF
Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVF 174
Query: 718 AXRYGKIAVXICF 756
++ KI V IC+
Sbjct: 175 QTKFAKIGVAICW 187
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 60.1 bits (139), Expect = 2e-09
Identities = 44/153 (28%), Positives = 69/153 (45%), Gaps = 7/153 (4%)
Frame = +1
Query: 319 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 492
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 493 TTTFLRELAIRYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNH-----IPR 657
+T L E++ R + I+ + E+ D L+NT V G + KHRK H IP
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPG 211
Query: 658 VGDFNESNYYMEGNTGHPVFAXRYGKIAVXICF 756
F ES G T + G+I + IC+
Sbjct: 212 KITFMESKTLTAGET-PTIVDTDVGRIGIGICY 243
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 60.1 bits (139), Expect = 2e-09
Identities = 44/153 (28%), Positives = 69/153 (45%), Gaps = 7/153 (4%)
Frame = +1
Query: 319 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 492
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 493 TTTFLRELAIRYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNH-----IPR 657
+T L E++ R + I+ + E+ D L+NT V G + KHRK H IP
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPG 211
Query: 658 VGDFNESNYYMEGNTGHPVFAXRYGKIAVXICF 756
F ES G T + G+I + IC+
Sbjct: 212 KITFMESKTLTAGET-PTIVDTDVGRIGIGICY 243
>At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1
[Mus musculus] GI:3228668; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 307
Score = 33.5 bits (73), Expect = 0.19
Identities = 39/146 (26%), Positives = 65/146 (44%), Gaps = 8/146 (5%)
Frame = +1
Query: 343 FNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAI 522
F +++ A G +ICF E F+F ++ + AE DGP LA
Sbjct: 54 FATCSRLVQEAALAGAKLICFPE----NFSFVGDKEGESVKIAEPL-DGPVMERYCSLA- 107
Query: 523 RYAMVIVS--SILER-DEKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNES 678
R + + +S ER D+ H L NT VVI D G + ++K H +P + ES
Sbjct: 108 RDSNIWLSLGGFQERFDDTH---LCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKES 164
Query: 679 NYYMEGNTGHPVFAXRYGKIAVXICF 756
++ + G V + G++ + +C+
Sbjct: 165 SFTVPGTKIVSVDSP-VGRLGLTVCY 189
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 33.1 bits (72), Expect = 0.25
Identities = 49/180 (27%), Positives = 72/180 (40%), Gaps = 13/180 (7%)
Frame = +1
Query: 256 PPRIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELW------ 417
P V+V I+Q S V D P A +K +K I A +G ++ F E +
Sbjct: 21 PSSTVRVTIVQSS-TVYNDTP------ATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPR 73
Query: 418 --NMPFAFCTREKQPWCEFAE---SA--EDGPTTTFLRELAIRYAMVIVSSILERDEKHS 576
A ++ EF SA GP L ELA + + +V +E+D
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKD---G 130
Query: 577 DILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFAXRYGKIAVXICF 756
L+ TA+ S G +GKHRK +P + + PV+ GKI IC+
Sbjct: 131 YTLYCTALFFSPQGQFLGKHRK-VMPT--SLERCIWGQGDGSTIPVYDTPIGKIGAAICW 187
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 32.3 bits (70), Expect = 0.44
Identities = 37/153 (24%), Positives = 64/153 (41%), Gaps = 13/153 (8%)
Frame = +1
Query: 337 AIFNKVKKIIDVAGQEGVNIICFQELW--------NMPFAFCTREKQPWCEFAE---SAE 483
A +K ++++ A + G ++ F E + A +R + +F + SA
Sbjct: 52 ATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAI 111
Query: 484 D--GPTTTFLRELAIRYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPR 657
D GP L +A +Y + +V ++ER+ L+ T + G +GKHRK +P
Sbjct: 112 DVPGPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHRK-LMPT 167
Query: 658 VGDFNESNYYMEGNTGHPVFAXRYGKIAVXICF 756
+ + PVF GKI IC+
Sbjct: 168 A--LERCIWGFGDGSTIPVFDTPIGKIGAAICW 198
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 32.3 bits (70), Expect = 0.44
Identities = 26/90 (28%), Positives = 40/90 (44%)
Frame = +1
Query: 487 GPTTTFLRELAIRYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGD 666
GP L ELA + + +V +E+D L+ TA+ S G +GKHRK +P
Sbjct: 97 GPEVEKLAELAGKNNVYLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRK-LMPT--S 150
Query: 667 FNESNYYMEGNTGHPVFAXRYGKIAVXICF 756
+ + PV+ GK+ IC+
Sbjct: 151 LERCIWGQGDGSTIPVYDTPIGKLGAAICW 180
>At5g06970.1 68418.m00789 expressed protein
Length = 1101
Score = 30.3 bits (65), Expect = 1.8
Identities = 14/59 (23%), Positives = 27/59 (45%)
Frame = -3
Query: 638 RCFPITFPVSLMTTAVFQSMSECFSSLSNIEDTITIAYLMASSRRKVVVGPSSADSANS 462
R I P + M ++ S LS +ED++ + ++ R K+V+ S + + S
Sbjct: 834 RSINIDVPATAMLCVQLNTLHYAVSQLSKLEDSMWLRWIAKKPREKIVIRKSMVEKSKS 892
>At5g67240.1 68418.m08475 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 745
Score = 29.1 bits (62), Expect = 4.1
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 495 HDLPSGTRHQVRNGDRVLDIRKGREAFGH 581
HD+PS H V NGD LD++ + G+
Sbjct: 441 HDVPSQELHGVLNGDFTLDVKPPKRKGGY 469
>At2g44760.1 68415.m05571 expressed protein
Length = 504
Score = 28.7 bits (61), Expect = 5.4
Identities = 23/89 (25%), Positives = 42/89 (47%), Gaps = 2/89 (2%)
Frame = +1
Query: 562 DEKHSDILWNTAVVISDTGNVI-GKHRKNHIP-RVGDFNESNYYMEGNTGHPVFAXRYGK 735
D D + N V++ D ++ G + + + VG+ +ESNYY+ P F ++ +
Sbjct: 257 DVMEEDSMINDVVMVFDVADMQDGSYSRGPVTIPVGEMSESNYYLT-----PTF--KFEQ 309
Query: 736 IAVXICFGRDHVLNWMMFGXNGXEIVXIR 822
V C R V++ + F G +I +R
Sbjct: 310 CLVKGCHKRLRVVHTIEFANGGADIQIMR 338
>At5g45720.1 68418.m05621 hypothetical protein
Length = 900
Score = 28.3 bits (60), Expect = 7.1
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = +1
Query: 601 VISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFAXR 726
V+ D+ IG+H +NHI + N+ N PV R
Sbjct: 9 VLKDSNGDIGEHLRNHIHLTNCIHLKNHMHNNNKQSPVLTDR 50
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 28.3 bits (60), Expect = 7.1
Identities = 44/180 (24%), Positives = 71/180 (39%), Gaps = 13/180 (7%)
Frame = +1
Query: 256 PPRIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQE--LWNMPF 429
P V+V I+Q S V D P A +K +K I A +G ++ F E + P
Sbjct: 21 PSTTVRVTIVQSS-TVYNDTP------ATIDKAEKYIVEAASKGAELVLFPEGFIGGYPR 73
Query: 430 AFC---------TREKQPWCEFAESA--EDGPTTTFLRELAIRYAMVIVSSILERDEKHS 576
F + + ++ SA GP L ++A + + +V +E K
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIE---KEG 130
Query: 577 DILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFAXRYGKIAVXICF 756
L+ T + S G +GKHRK +P + + PV+ GK+ IC+
Sbjct: 131 YTLYCTVLFFSPQGQFLGKHRK-LMPT--SLERCIWGQGDGSTIPVYDTPIGKLGAAICW 187
>At5g40740.1 68418.m04944 expressed protein
Length = 741
Score = 27.9 bits (59), Expect = 9.4
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +1
Query: 556 ERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESN 681
ER+ + ++ +++ NVIG N PRVG F SN
Sbjct: 8 ERELELESAMYTNCLLLGLDPNVIGLGASNGTPRVGLFRHSN 49
>At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N
domain-containing protein contains Pfam profile PF00627:
UBA/TS-N domain
Length = 649
Score = 27.9 bits (59), Expect = 9.4
Identities = 17/38 (44%), Positives = 25/38 (65%), Gaps = 2/38 (5%)
Frame = -3
Query: 614 VSLMTTAVFQSMSECFSSLSNIEDT-ITIAYL-MASSR 507
+ L + + FQ+ FSSL+NI+ T T+A L MASS+
Sbjct: 482 IGLPSQSQFQASGSSFSSLTNIDPTDFTVANLPMASSQ 519
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,622,731
Number of Sequences: 28952
Number of extensions: 370688
Number of successful extensions: 1041
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 996
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1036
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2048424000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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