BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BmNP01_FL5_I05 (899 letters) Database: tribolium 336 sequences; 122,585 total letters Searching.......................................................done Score E Sequences producing significant alignments: (bits) Value EF127808-1|ABL67945.1| 311|Tribolium castaneum nicotinic acetyl... 43 3e-06 EF127807-1|ABL67944.1| 311|Tribolium castaneum nicotinic acetyl... 43 3e-06 EF127806-1|ABL67943.1| 311|Tribolium castaneum nicotinic acetyl... 41 2e-05 EF127810-1|ABL67947.1| 311|Tribolium castaneum nicotinic acetyl... 39 5e-05 EF127809-1|ABL67946.1| 311|Tribolium castaneum nicotinic acetyl... 39 5e-05 AM292361-1|CAL23173.2| 373|Tribolium castaneum gustatory recept... 22 5.7 >EF127808-1|ABL67945.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 3 protein. Length = 311 Score = 43.2 bits (97), Expect = 3e-06 Identities = 28/97 (28%), Positives = 47/97 (48%), Gaps = 2/97 (2%) Frame = +3 Query: 141 ESPPDSKIVVNLTLHLRHANIRESESTVRILADLQMNWIDKRLXWNAGEW-GCSTWLVSS 317 ES P ++ LTL + ++ E + A L + W D L WN E+ G ++ Sbjct: 25 ESEP-LEVKFGLTLQ-QIIDVDEKNQILTTNAWLNLEWNDYNLKWNESEYGGVKDLRITP 82 Query: 318 ERLWRPDVVLLNAA-ATTAGDYALRARVSNNGSVSWI 425 +LW+PDV++ N+A G + V +NGS ++ Sbjct: 83 NKLWKPDVLMYNSADEGFDGTFQTNVVVKHNGSCLYV 119 Score = 27.9 bits (59), Expect = 0.11 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = +2 Query: 455 MQLDNWPNDMQTCTFKFGSRMHNSDEMDFVID 550 M + +P D Q C KFGS ++ +++D V++ Sbjct: 130 MDITWFPFDDQHCDMKFGSWTYDGNQLDLVLN 161 >EF127807-1|ABL67944.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 2 protein. Length = 311 Score = 43.2 bits (97), Expect = 3e-06 Identities = 28/97 (28%), Positives = 47/97 (48%), Gaps = 2/97 (2%) Frame = +3 Query: 141 ESPPDSKIVVNLTLHLRHANIRESESTVRILADLQMNWIDKRLXWNAGEW-GCSTWLVSS 317 ES P ++ LTL + ++ E + A L + W D L WN E+ G ++ Sbjct: 25 ESEP-LEVKFGLTLQ-QIIDVDEKNQILTTNAWLNLEWNDYNLKWNESEYGGVKDLRITP 82 Query: 318 ERLWRPDVVLLNAA-ATTAGDYALRARVSNNGSVSWI 425 +LW+PDV++ N+A G + V +NGS ++ Sbjct: 83 NKLWKPDVLMYNSADEGFDGTFQTNVVVKHNGSCLYV 119 Score = 27.1 bits (57), Expect = 0.20 Identities = 10/27 (37%), Positives = 18/27 (66%) Frame = +2 Query: 470 WPNDMQTCTFKFGSRMHNSDEMDFVID 550 +P D Q C KFGS ++ +++D V++ Sbjct: 135 FPFDDQHCDMKFGSWTYDGNQLDLVLN 161 >EF127806-1|ABL67943.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 1 protein. Length = 311 Score = 40.7 bits (91), Expect = 2e-05 Identities = 27/97 (27%), Positives = 46/97 (47%), Gaps = 2/97 (2%) Frame = +3 Query: 141 ESPPDSKIVVNLTLHLRHANIRESESTVRILADLQMNWIDKRLXWNAGEW-GCSTWLVSS 317 ES P ++ LTL + ++ E + A L + W D L WN E+ G ++ Sbjct: 25 ESEP-LEVKFGLTLQ-QIIDVDEKNQILTTNAWLNLEWNDYNLKWNESEYGGVKDLRITP 82 Query: 318 ERLWRPDVVLLNAA-ATTAGDYALRARVSNNGSVSWI 425 +LW+PDV++ N+A G + V + GS ++ Sbjct: 83 NKLWKPDVLMYNSADEGFDGTFQTNVVVKHGGSCLYV 119 Score = 27.9 bits (59), Expect = 0.11 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = +2 Query: 455 MQLDNWPNDMQTCTFKFGSRMHNSDEMDFVID 550 M + +P D Q C KFGS ++ +++D V++ Sbjct: 130 MDITWFPFDDQHCDMKFGSWTYDGNQLDLVLN 161 >EF127810-1|ABL67947.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 5 protein. Length = 311 Score = 39.1 bits (87), Expect = 5e-05 Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 2/64 (3%) Frame = +3 Query: 240 LQMNWIDKRLXWNAGEWGCSTWL-VSSERLWRPDVVLLNAA-ATTAGDYALRARVSNNGS 413 L + W D L WN E+G L ++ +LW+PDV++ N+A G + V + GS Sbjct: 56 LSLEWNDYNLKWNESEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTFQTNVVVKHGGS 115 Query: 414 VSWI 425 ++ Sbjct: 116 CLYV 119 Score = 27.9 bits (59), Expect = 0.11 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = +2 Query: 455 MQLDNWPNDMQTCTFKFGSRMHNSDEMDFVID 550 M + +P D Q C KFGS ++ +++D V++ Sbjct: 130 MDITWFPFDDQHCDMKFGSWTYDGNQLDLVLN 161 >EF127809-1|ABL67946.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 4 protein. Length = 311 Score = 39.1 bits (87), Expect = 5e-05 Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 2/64 (3%) Frame = +3 Query: 240 LQMNWIDKRLXWNAGEWGCSTWL-VSSERLWRPDVVLLNAA-ATTAGDYALRARVSNNGS 413 L + W D L WN E+G L ++ +LW+PDV++ N+A G + V + GS Sbjct: 56 LSLEWNDYNLKWNESEYGGVKDLRITPNKLWKPDVLMYNSADEGFDGTFQTNVVVKHGGS 115 Query: 414 VSWI 425 ++ Sbjct: 116 CLYV 119 Score = 27.9 bits (59), Expect = 0.11 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = +2 Query: 455 MQLDNWPNDMQTCTFKFGSRMHNSDEMDFVID 550 M + +P D Q C KFGS ++ +++D V++ Sbjct: 130 MDITWFPFDDQHCDMKFGSWTYDGNQLDLVLN 161 >AM292361-1|CAL23173.2| 373|Tribolium castaneum gustatory receptor candidate 40 protein. Length = 373 Score = 22.2 bits (45), Expect = 5.7 Identities = 7/11 (63%), Positives = 8/11 (72%) Frame = +2 Query: 449 ISMQLDNWPND 481 + M LDNW ND Sbjct: 295 VEMALDNWKND 305 Database: tribolium Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 122,585 Number of sequences in database: 336 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 158,931 Number of Sequences: 336 Number of extensions: 3391 Number of successful extensions: 19 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 14 length of database: 122,585 effective HSP length: 57 effective length of database: 103,433 effective search space used: 25030786 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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