Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_FL5_E22
(854 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 64 3e-11
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 40 4e-04
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 32 0.090
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 29 0.84
SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 28 1.5
SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual 27 4.5
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 26 7.8
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 26 7.8
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 63.7 bits (148), Expect = 3e-11
Identities = 37/120 (30%), Positives = 66/120 (55%)
Frame = +3
Query: 396 NIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEK 575
N+I F EL + C + + AE A +GP+ + LA KY + I+ E++EK
Sbjct: 39 NLILFPELITSGYE-CGNT---FTQIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEK 94
Query: 576 HSDILWNTAVVISDTGNVIGKHRKNHIPXVGDFNESNYYMERYTGHPVFATXYGKIAVNI 755
S+I++N+ + I++ GN+ G +RK H+ F+ + ++ + P+F T +GK+ V I
Sbjct: 95 QSNIIYNSCIYITENGNLGGVYRKVHL-----FDTERKHFKKGSDFPIFETSFGKLGVMI 149
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 39.9 bits (89), Expect = 4e-04
Identities = 44/170 (25%), Positives = 74/170 (43%), Gaps = 5/170 (2%)
Frame = +3
Query: 267 RIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCT 446
R ++G++Q +A D+ N Q + K+++ A + G N+I E++N P+ T
Sbjct: 42 RAFRIGLVQ--LANTKDKSENLQLARL-----KVLEAA-KNGSNVIVLPEIFNSPYG--T 91
Query: 447 REKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 626
+ E E E P+ L +A + + E+ L+NTA+V +G
Sbjct: 92 GYFNQYAEPIE--ESSPSYQALSSMAKDTKTYLFGGSIP--ERKDGKLYNTAMVFDPSGK 147
Query: 627 VIGKHRKNH-----IPXVGDFNESNYYMERYTGHPVFATXYGKIAVNIXF 761
+I HRK H IP F ES+ + + T YGK + I +
Sbjct: 148 LIAVHRKIHLFDIDIPGGVSFRESD-SLSPGDAMTMVDTEYGKFGLGICY 196
>SPBC336.05c |||S-adenosylmethionine-
dependentmethyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 378
Score = 32.3 bits (70), Expect = 0.090
Identities = 21/64 (32%), Positives = 31/64 (48%)
Frame = -2
Query: 508 KVVVGPSSADSANSHHGCFSLVQNAKGIFHNSWKQMMLTPSWPATSMIFLTLLKIAFFCS 329
++ V S ++A SH CF QN+ + + + +P T F+ LLK AFF
Sbjct: 232 QIAVFHQSKNNAASH--CFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDH 289
Query: 328 LTGR 317
L GR
Sbjct: 290 LFGR 293
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 29.1 bits (62), Expect = 0.84
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = +3
Query: 480 SAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDIL 590
SAE+ + + + +++VS++LE DEKH D++
Sbjct: 979 SAENTTSFSIFAAQGLTDFLIVVSNLLEMDEKHVDVV 1015
>SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1496
Score = 28.3 bits (60), Expect = 1.5
Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
Frame = -1
Query: 290 NYSYLHNSRRSGLLVLGRESVC---GDVEVSLLSCSDRGF 180
++S N++R+G L +G ++VC GD + LSC G+
Sbjct: 885 DFSRSVNNQRNGHLTVGSDAVCLSLGDSQFHRLSCDSVGY 924
>SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1202
Score = 26.6 bits (56), Expect = 4.5
Identities = 29/116 (25%), Positives = 48/116 (41%)
Frame = -1
Query: 461 RLLLPCAEREGHIPQLLETDDVNTLLAGNIDDLLDFIENCFLLLVDWTIGGHRDGMLNYS 282
R LL C +R P +L DV ++DD + F+ T GH++ + N S
Sbjct: 119 RALLSCCKRSKD-PSILFPTDVPC----SLDDDVSFL----------TFKGHKNHLENRS 163
Query: 281 YLHNSRRSGLLVLGRESVCGDVEVSLLSCSDRGFFQFDFKVIPPPKMNSVELFQVA 114
+ H+S V+ E + L DR K++ P N ++L +V+
Sbjct: 164 FFHDSESDNFKVVLSNCAINSKEDNNLVTEDR--VNLGAKLLLVPVQNLIKLLKVS 217
>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 502
Score = 25.8 bits (54), Expect = 7.8
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3
Query: 99 NNLTGRDLEEFNRIHFGRRNNLEI 170
NN+ R +EE N I G+R LE+
Sbjct: 8 NNILKRHIEEDNNIDNGKRKKLEL 31
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +3
Query: 69 ETHSLESII-NNNLTGRDLEEFNRIHFGRRNNLEIKLK 179
E H +E +I + N+T DL F + FG+ N ++ K
Sbjct: 369 EFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFK 406
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,212,809
Number of Sequences: 5004
Number of extensions: 65251
Number of successful extensions: 219
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 205
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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