Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_FL5_E22
(854 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 272 1e-73
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 64 1e-10
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 63 3e-10
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 54 9e-08
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 54 9e-08
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 33 0.32
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 33 0.32
At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas... 31 0.74
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 31 1.3
At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to ... 31 1.3
At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to ... 31 1.3
At5g06970.1 68418.m00789 expressed protein 30 1.7
At5g67240.1 68418.m08475 exonuclease family protein contains exo... 29 3.9
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 29 5.2
At1g34070.1 68414.m04224 hypothetical protein 28 6.9
At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N domain-... 28 9.1
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 272 bits (668), Expect = 1e-73
Identities = 135/240 (56%), Positives = 166/240 (69%), Gaps = 3/240 (1%)
Frame = +3
Query: 78 SLESIINNNLTGRDLEEFNRIHFGR---RNNLEIKLKESSIXXXXXXXXXXXXXXFPAKD 248
SL +++ NL +E NR+ GR R+ +I L ES+ F A
Sbjct: 22 SLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAASFSADK 81
Query: 249 EQTRPPRIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNM 428
EQ R PR+V+VG+IQ+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W M
Sbjct: 82 EQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTM 141
Query: 429 PFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVV 608
PFAFCTRE++ WCEFAE DG +T FL+ELA KY MVIVS ILERD H ++LWNTAV+
Sbjct: 142 PFAFCTRERR-WCEFAEPV-DGESTKFLQELAKKYNMVIVSPILERDIDHGEVLWNTAVI 199
Query: 609 ISDTGNVIGKHRKNHIPXVGDFNESNYYMERYTGHPVFATXYGKIAVNIXFGRXXXLNWM 788
I + GN+IGKHRKNHIP VGDFNES YYME TGHPVF T +GKIAVNI +GR LNW+
Sbjct: 200 IGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETVFGKIAVNICYGRHHPLNWL 259
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 64.1 bits (149), Expect = 1e-10
Identities = 41/133 (30%), Positives = 71/133 (53%), Gaps = 1/133 (0%)
Frame = +3
Query: 360 KKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAM 539
++++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA + +
Sbjct: 31 ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89
Query: 540 VI-VSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPXVGDFNESNYYMERYTGHP 716
VI VS E + H +N+ +I G +G +RK+HIP + E Y+ TG
Sbjct: 90 VIPVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFK 145
Query: 717 VFATXYGKIAVNI 755
VF T + KI V I
Sbjct: 146 VFQTKFAKIGVAI 158
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 62.9 bits (146), Expect = 3e-10
Identities = 41/131 (31%), Positives = 69/131 (52%), Gaps = 1/131 (0%)
Frame = +3
Query: 366 IIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVI 545
++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA + +VI
Sbjct: 60 LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118
Query: 546 -VSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPXVGDFNESNYYMERYTGHPVF 722
VS E + H +N+ +I G +G +RK+HIP + E Y+ TG VF
Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVF 174
Query: 723 ATXYGKIAVNI 755
T + KI V I
Sbjct: 175 QTKFAKIGVAI 185
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 54.4 bits (125), Expect = 9e-08
Identities = 42/153 (27%), Positives = 68/153 (44%), Gaps = 7/153 (4%)
Frame = +3
Query: 324 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 497
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 498 TTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNH-----IPX 662
+T L E++ + + I+ + E+ D L+NT V G + KHRK H IP
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPG 211
Query: 663 VGDFNESNYYMERYTGHPVFATXYGKIAVNIXF 761
F ES T + T G+I + I +
Sbjct: 212 KITFMESKTLTAGET-PTIVDTDVGRIGIGICY 243
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 54.4 bits (125), Expect = 9e-08
Identities = 42/153 (27%), Positives = 68/153 (44%), Gaps = 7/153 (4%)
Frame = +3
Query: 324 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 497
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 498 TTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNH-----IPX 662
+T L E++ + + I+ + E+ D L+NT V G + KHRK H IP
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHLFDIDIPG 211
Query: 663 VGDFNESNYYMERYTGHPVFATXYGKIAVNIXF 761
F ES T + T G+I + I +
Sbjct: 212 KITFMESKTLTAGET-PTIVDTDVGRIGIGICY 243
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 32.7 bits (71), Expect = 0.32
Identities = 42/142 (29%), Positives = 59/142 (41%), Gaps = 13/142 (9%)
Frame = +3
Query: 261 PPRIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELW------ 422
P V+V I+Q S V D P A +K +K I A +G ++ F E +
Sbjct: 21 PSSTVRVTIVQSS-TVYNDTP------ATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPR 73
Query: 423 --NMPFAFCTREKQPWCEFAE---SA--EDGPTTTFLRELAIKYAMVIVSSILERDEKHS 581
A ++ EF SA GP L ELA K + +V +E+D
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKD---G 130
Query: 582 DILWNTAVVISDTGNVIGKHRK 647
L+ TA+ S G +GKHRK
Sbjct: 131 YTLYCTALFFSPQGQFLGKHRK 152
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 32.7 bits (71), Expect = 0.32
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = +3
Query: 492 GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 647
GP L ELA K + +V +E+D L+ TA+ S G +GKHRK
Sbjct: 97 GPEVEKLAELAGKNNVYLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRK 145
>At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1
[Mus musculus] GI:3228668; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 307
Score = 31.5 bits (68), Expect = 0.74
Identities = 34/122 (27%), Positives = 52/122 (42%), Gaps = 2/122 (1%)
Frame = +3
Query: 348 FNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAI 527
F +++ A G +ICF E F+F ++ + AE DGP LA
Sbjct: 54 FATCSRLVQEAALAGAKLICFPE----NFSFVGDKEGESVKIAEPL-DGPVMERYCSLAR 108
Query: 528 KYAMVI-VSSILER-DEKHSDILWNTAVVISDTGNVIGKHRKNHIPXVGDFNESNYYMER 701
+ + + ER D+ H L NT VVI D G + ++K H+ V S+Y
Sbjct: 109 DSNIWLSLGGFQERFDDTH---LCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKESS 165
Query: 702 YT 707
+T
Sbjct: 166 FT 167
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 30.7 bits (66), Expect = 1.3
Identities = 29/115 (25%), Positives = 51/115 (44%), Gaps = 13/115 (11%)
Frame = +3
Query: 342 AIFNKVKKIIDVAGQEGVNIICFQELW--------NMPFAFCTREKQPWCEFAE---SAE 488
A +K ++++ A + G ++ F E + A +R + +F + SA
Sbjct: 52 ATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAI 111
Query: 489 D--GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 647
D GP L +A KY + +V ++ER+ L+ T + G +GKHRK
Sbjct: 112 DVPGPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHRK 163
>At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 383
Score = 30.7 bits (66), Expect = 1.3
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +3
Query: 609 ISDTGNVIGKHRKNHIPXVGDFNESNYYMERYTGHPVFATXYGKIAVN 752
I+DTGN++G +NH+P Y E + HP + G+I ++
Sbjct: 39 ITDTGNLVGLSDRNHLPVTAFLP----YGETFFHHPTGRSCNGRIIID 82
>At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 250
Score = 30.7 bits (66), Expect = 1.3
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +3
Query: 609 ISDTGNVIGKHRKNHIPXVGDFNESNYYMERYTGHPVFATXYGKIAVN 752
I+DTGN++G +NH+P Y E + HP + G+I ++
Sbjct: 39 ITDTGNLVGLSDRNHLPVTAFLP----YGETFFHHPTGRSCNGRIIID 82
>At5g06970.1 68418.m00789 expressed protein
Length = 1101
Score = 30.3 bits (65), Expect = 1.7
Identities = 14/59 (23%), Positives = 27/59 (45%)
Frame = -2
Query: 643 RCFPITFPVSLMTTAVFQSMSECFSSLSNIEDTITIAYLMASSRRKVVVGPSSADSANS 467
R I P + M ++ S LS +ED++ + ++ R K+V+ S + + S
Sbjct: 834 RSINIDVPATAMLCVQLNTLHYAVSQLSKLEDSMWLRWIAKKPREKIVIRKSMVEKSKS 892
>At5g67240.1 68418.m08475 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 745
Score = 29.1 bits (62), Expect = 3.9
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +2
Query: 500 HDLPSGTRHQVRNGDRVLDIRKGREAFGH 586
HD+PS H V NGD LD++ + G+
Sbjct: 441 HDVPSQELHGVLNGDFTLDVKPPKRKGGY 469
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 28.7 bits (61), Expect = 5.2
Identities = 38/142 (26%), Positives = 58/142 (40%), Gaps = 13/142 (9%)
Frame = +3
Query: 261 PPRIVKVGIIQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQE--LWNMPF 434
P V+V I+Q S V D P A +K +K I A +G ++ F E + P
Sbjct: 21 PSTTVRVTIVQSS-TVYNDTP------ATIDKAEKYIVEAASKGAELVLFPEGFIGGYPR 73
Query: 435 AFC---------TREKQPWCEFAESA--EDGPTTTFLRELAIKYAMVIVSSILERDEKHS 581
F + + ++ SA GP L ++A K + +V +E K
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIE---KEG 130
Query: 582 DILWNTAVVISDTGNVIGKHRK 647
L+ T + S G +GKHRK
Sbjct: 131 YTLYCTVLFFSPQGQFLGKHRK 152
>At1g34070.1 68414.m04224 hypothetical protein
Length = 308
Score = 28.3 bits (60), Expect = 6.9
Identities = 19/68 (27%), Positives = 30/68 (44%), Gaps = 3/68 (4%)
Frame = +3
Query: 603 VVISDTGNVIG-KHRKNHIPXVGDFNESNY--YMERYTGHPVFATXYGKIAVNIXFGRXX 773
VV+ + + G + K+HIP + D ESNY + E + H + G I +
Sbjct: 2 VVVMNPEQIYGVSNIKSHIPVMLDIEESNYDAWRELFLTHCLSFDVMGHIDGTLLPTNAN 61
Query: 774 XLNWMMSD 797
+NW D
Sbjct: 62 DVNWQKRD 69
>At2g33010.1 68415.m04045 ubiquitin-associated (UBA)/TS-N
domain-containing protein contains Pfam profile PF00627:
UBA/TS-N domain
Length = 649
Score = 27.9 bits (59), Expect = 9.1
Identities = 17/38 (44%), Positives = 25/38 (65%), Gaps = 2/38 (5%)
Frame = -2
Query: 619 VSLMTTAVFQSMSECFSSLSNIEDT-ITIAYL-MASSR 512
+ L + + FQ+ FSSL+NI+ T T+A L MASS+
Sbjct: 482 IGLPSQSQFQASGSSFSSLTNIDPTDFTVANLPMASSQ 519
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,136,654
Number of Sequences: 28952
Number of extensions: 355906
Number of successful extensions: 1030
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 982
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1025
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1989897600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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