BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BmNP01_FL5_E15 (854 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g05130.1 68418.m00544 SNF2 domain-containing protein / helica... 29 3.9 At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 29 5.2 At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 29 5.2 At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 28 6.9 At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 28 6.9 At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 28 6.9 At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 28 6.9 >At5g05130.1 68418.m00544 SNF2 domain-containing protein / helicase domain-containing protein / RING finger domain-containing protein similar to transcription factor RUSH-1alpha [Oryctolagus cuniculus] GI:1655930; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain, PF00097: Zinc finger, C3HC4 type (RING finger) Length = 862 Score = 29.1 bits (62), Expect = 3.9 Identities = 12/58 (20%), Positives = 32/58 (55%), Gaps = 1/58 (1%) Frame = -3 Query: 189 LKCRVRLTTILESGGDSRSYVARSESIM-RDSDVAFSHSAALAIAQVRRNGNQNNISR 19 + C++ + LE+ +S ++R ++ +SD +F S A+ + + NG++ ++ + Sbjct: 126 IPCQIHVFAKLEASSTVKSTISRGGLVLISESDTSFGLSEAVVVKEQMGNGDKRSVDK 183 >At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (PRCFC) identical to 20S proteasome beta subunit PBB2 [Arabidopsis thaliana] GI:3421104, cDNA proteasome subunit prcfc GI:2511575 Length = 274 Score = 28.7 bits (61), Expect = 5.2 Identities = 17/56 (30%), Positives = 28/56 (50%) Frame = +2 Query: 26 ILFWLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 193 I + P + C +AA+ E T M+ S L + ++ DS++V LTL +H Sbjct: 73 IHYMAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVVTALTLLKKH 125 >At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (PRCFC) identical to 20S proteasome beta subunit PBB2 [Arabidopsis thaliana] GI:3421104, cDNA proteasome subunit prcfc GI:2511575 Length = 274 Score = 28.7 bits (61), Expect = 5.2 Identities = 17/56 (30%), Positives = 28/56 (50%) Frame = +2 Query: 26 ILFWLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 193 I + P + C +AA+ E T M+ S L + ++ DS++V LTL +H Sbjct: 73 IHYMAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVVTALTLLKKH 125 >At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A subunit region (PF03178); contains weak WD-40 repeat (PF00400); similar to Splicing factor 3B subunit 3 (SF3b130)/spliceosomal protein/Splicing factor 3B subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens, EMBL:HSAJ1443_1 Length = 1214 Score = 28.3 bits (60), Expect = 6.9 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = -1 Query: 392 GRGERSPRRWLQPRLAVPRQASTSVPRTPA 303 GRG RS R L+P LA+ A + +P P+ Sbjct: 426 GRGPRSSLRILRPGLAITEMAVSQLPGQPS 455 >At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A subunit region (PF03178); contains weak WD-40 repeat (PF00400); similar to Splicing factor 3B subunit 3 (SF3b130)/spliceosomal protein/Splicing factor 3B subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens, EMBL:HSAJ1443_1 Length = 1214 Score = 28.3 bits (60), Expect = 6.9 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = -1 Query: 392 GRGERSPRRWLQPRLAVPRQASTSVPRTPA 303 GRG RS R L+P LA+ A + +P P+ Sbjct: 426 GRGPRSSLRILRPGLAITEMAVSQLPGQPS 455 >At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) identical to 20S proteasome beta subunit PBB1 (PBB1) GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2), 677-692 (1998)); contains Pfam profile: PF00227 proteasome A-type and B-type; Length = 273 Score = 28.3 bits (60), Expect = 6.9 Identities = 16/56 (28%), Positives = 28/56 (50%) Frame = +2 Query: 26 ILFWLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 193 I + P + C +AA+ E T M+ S L + ++ DS+++ LTL +H Sbjct: 73 IHYMAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVITALTLLKKH 125 >At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) identical to 20S proteasome beta subunit PBB1 (PBB1) GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2), 677-692 (1998)); contains Pfam profile: PF00227 proteasome A-type and B-type; Length = 267 Score = 28.3 bits (60), Expect = 6.9 Identities = 16/56 (28%), Positives = 28/56 (50%) Frame = +2 Query: 26 ILFWLPFLLTCAIASAAECENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRH 193 I + P + C +AA+ E T M+ S L + ++ DS+++ LTL +H Sbjct: 73 IHYMAPNIYCCGAGTAADTEAVTD---MVSSQLRLHRYQTGRDSRVITALTLLKKH 125 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,059,388 Number of Sequences: 28952 Number of extensions: 370553 Number of successful extensions: 1066 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 998 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1062 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1989897600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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