Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_FL5_D22
(861 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 250 7e-67
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 249 2e-66
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 30 1.7
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 30 1.7
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 29 4.0
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 29 4.0
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 28 7.0
At1g18670.1 68414.m02330 protein kinase family protein contains ... 28 7.0
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 9.2
At5g01010.1 68418.m00001 expressed protein 28 9.2
At2g47550.1 68415.m05934 pectinesterase family protein contains ... 28 9.2
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 250 bits (613), Expect = 7e-67
Identities = 120/218 (55%), Positives = 144/218 (66%), Gaps = 1/218 (0%)
Frame = +2
Query: 143 TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSGQPYCVSKEAGHQTSAESWGTGRAVAR 322
T Q LP V AP+RPD+VN VH +S NS QPY VSK+AGHQTSAESWGTGRAV+R
Sbjct: 22 TDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKAGHQTSAESWGTGRAVSR 81
Query: 323 IPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXXXXXXXXXXXXXXXX 502
IPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 82 IPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPA 141
Query: 503 XXQARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGK 682
ARGH IE +PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGK
Sbjct: 142 LVMARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGK 201
Query: 683 MRNRRRIQRKGPLIIFNKDQG-LTRAFRNIPGVXLLNV 793
MRNRR I RKGPL++F + + +AFRN+PGV L +V
Sbjct: 202 MRNRRYISRKGPLVVFGTEGAKIVKAFRNLPGVELCHV 239
Score = 39.1 bits (87), Expect = 0.004
Identities = 18/27 (66%), Positives = 19/27 (70%)
Frame = +1
Query: 769 PGCGXPECNKLNLLKLAPGXHLGRFVI 849
PG +LNLLKLAPG HLGRFVI
Sbjct: 232 PGVELCHVERLNLLKLAPGGHLGRFVI 258
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 249 bits (610), Expect = 2e-66
Identities = 119/218 (54%), Positives = 145/218 (66%), Gaps = 1/218 (0%)
Frame = +2
Query: 143 TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSGQPYCVSKEAGHQTSAESWGTGRAVAR 322
T Q + LP V AP+RPD+VN VH +S NS QPY VSK+AGHQTSAESWGTGRAV+R
Sbjct: 21 TDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKAGHQTSAESWGTGRAVSR 80
Query: 323 IPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXXXXXXXXXXXXXXXX 502
IPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 81 IPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPA 140
Query: 503 XXQARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGK 682
ARGH IE +PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGK
Sbjct: 141 LVMARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGK 200
Query: 683 MRNRRRIQRKGPLIIFNKD-QGLTRAFRNIPGVXLLNV 793
MRNRR I RKGPL+++ + + +AFRN+PGV L +V
Sbjct: 201 MRNRRYISRKGPLVVYGTEGSKIVKAFRNLPGVELCHV 238
Score = 39.1 bits (87), Expect = 0.004
Identities = 18/27 (66%), Positives = 19/27 (70%)
Frame = +1
Query: 769 PGCGXPECNKLNLLKLAPGXHLGRFVI 849
PG +LNLLKLAPG HLGRFVI
Sbjct: 231 PGVELCHVERLNLLKLAPGGHLGRFVI 257
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 30.3 bits (65), Expect = 1.7
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +1
Query: 388 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCCYRRPSARSG*RTHY*KDSRASLGCSRQ 567
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC-PSPLSQSSKQHHIRLDNHFDTSTPRS 523
Query: 568 S 570
S
Sbjct: 524 S 524
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 30.3 bits (65), Expect = 1.7
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +1
Query: 388 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCCYRRPSARSG*RTHY*KDSRASLGCSRQ 567
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC-PSPLSQSSKQHHIRLDNHFDTSTPRS 522
Query: 568 S 570
S
Sbjct: 523 S 523
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 29.1 bits (62), Expect = 4.0
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -2
Query: 404 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 285
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 29.1 bits (62), Expect = 4.0
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -2
Query: 404 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 285
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 28.3 bits (60), Expect = 7.0
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = -1
Query: 594 CLVLLISWTLSATTKGSSGIFS 529
CLVLL+S +A TK SGIF+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFT 30
>At1g18670.1 68414.m02330 protein kinase family protein contains
Protein kinases ATP-binding region signature,
PROSITE:PS00107 and Serine/Threonine protein kinases
active-site signature, PROSITE:PS00108
Length = 662
Score = 28.3 bits (60), Expect = 7.0
Identities = 18/54 (33%), Positives = 26/54 (48%)
Frame = +1
Query: 505 RSG*RTHY*KDSRASLGCSRQSPRDQQDQTGCHLPEAPQGMV*YP*GVQVSASS 666
R+G H DS ++L Q P + + H+ A QG V + +QVS SS
Sbjct: 491 RNGHSVHNSIDSDSTLFEKMQKPSNHEKDEASHVKNASQGDVPFSGPLQVSVSS 544
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 9.2
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -2
Query: 392 HHDTCYRRHPDRTYEYHHHGHAEF 321
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At5g01010.1 68418.m00001 expressed protein
Length = 409
Score = 27.9 bits (59), Expect = 9.2
Identities = 16/45 (35%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Frame = -3
Query: 289 STGLVTSLLAHAVGLPRVLGHRNVNIIDQVR-TDGRLEHEREGLG 158
+TG+ +L+ + VG+P+VL ++ I Q+ DG +E +RE G
Sbjct: 203 ATGVYKTLVKYLVGVPQVL----LDFIRQINDDDGPMEEQRERYG 243
>At2g47550.1 68415.m05934 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 560
Score = 27.9 bits (59), Expect = 9.2
Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 2/79 (2%)
Frame = -3
Query: 754 ASQTLILVED--YEGPLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGLV 581
A T+ +ED + LT+D + T STKTL L +D+ L A ++ +
Sbjct: 93 AKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQT--CL 150
Query: 580 DLLDFVGYNQGKLGNLFNN 524
+ L G G+LFN+
Sbjct: 151 EGLKSTASENGLSGDLFND 169
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,329,772
Number of Sequences: 28952
Number of extensions: 378881
Number of successful extensions: 1197
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1096
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1182
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2009406400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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