BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP01_FL5_D16
(857 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 30 0.37
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 27 4.5
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 7.9
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 30.3 bits (65), Expect = 0.37
Identities = 23/88 (26%), Positives = 41/88 (46%), Gaps = 1/88 (1%)
Frame = +2
Query: 200 FNKIMSTLKLISEVRSGLYSHWRDVFEHVRSTAKIKHYADQNTSSCTPQEREC-SIRKSC 376
F+K++ST IS + L WRD +HV + + +S C
Sbjct: 384 FSKMLST---ISNEITNLQGDWRDQLDHVEFLQEHLGPLEVELASVKVLYDNCFQAGIEE 440
Query: 377 NNVSVTSMEDLSHEEIVTSYVLAHVAQF 460
N+ ++ S EDL HE +T+ ++A+ ++
Sbjct: 441 NDYTMFSYEDLEHEFGITANIIANKIKY 468
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 26.6 bits (56), Expect = 4.5
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +2
Query: 377 NNVSVTSMEDLSHEEIVTSYVLAHVAQFDCRRHHMAFTNGNT 502
+N+SVT+ L + +V++Y L CR AFTNG T
Sbjct: 853 SNISVTNENRLQYIHLVSNYYLNARLSRQCR----AFTNGFT 890
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 7.9
Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 10/78 (12%)
Frame = -3
Query: 681 IHSLMSWFTVLVPYNSS----SSHWLKNDSCRN----YPLVMTDPLLRS--KFHLRFALA 532
++SL+SW + +NS S+ +L+ D C+N Y ++ L + FHL A
Sbjct: 53 VNSLLSWCMLSSWFNSKLSKLSAGYLQFD-CQNDGKCYSVIAVHRLSFTLVMFHLFLAFI 111
Query: 531 LISCNSLLRVVFPLVNAM 478
L CN+ RV + N +
Sbjct: 112 LSLCNTRSRVAIKIQNGL 129
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,344,999
Number of Sequences: 5004
Number of extensions: 65664
Number of successful extensions: 157
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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