BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BmNP01_FL5_D16 (857 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 30 0.37 SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 27 4.5 SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 7.9 >SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr 1|||Manual Length = 621 Score = 30.3 bits (65), Expect = 0.37 Identities = 23/88 (26%), Positives = 41/88 (46%), Gaps = 1/88 (1%) Frame = +2 Query: 200 FNKIMSTLKLISEVRSGLYSHWRDVFEHVRSTAKIKHYADQNTSSCTPQEREC-SIRKSC 376 F+K++ST IS + L WRD +HV + + +S C Sbjct: 384 FSKMLST---ISNEITNLQGDWRDQLDHVEFLQEHLGPLEVELASVKVLYDNCFQAGIEE 440 Query: 377 NNVSVTSMEDLSHEEIVTSYVLAHVAQF 460 N+ ++ S EDL HE +T+ ++A+ ++ Sbjct: 441 NDYTMFSYEDLEHEFGITANIIANKIKY 468 >SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1029 Score = 26.6 bits (56), Expect = 4.5 Identities = 16/42 (38%), Positives = 23/42 (54%) Frame = +2 Query: 377 NNVSVTSMEDLSHEEIVTSYVLAHVAQFDCRRHHMAFTNGNT 502 +N+SVT+ L + +V++Y L CR AFTNG T Sbjct: 853 SNISVTNENRLQYIHLVSNYYLNARLSRQCR----AFTNGFT 890 >SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 441 Score = 25.8 bits (54), Expect = 7.9 Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 10/78 (12%) Frame = -3 Query: 681 IHSLMSWFTVLVPYNSS----SSHWLKNDSCRN----YPLVMTDPLLRS--KFHLRFALA 532 ++SL+SW + +NS S+ +L+ D C+N Y ++ L + FHL A Sbjct: 53 VNSLLSWCMLSSWFNSKLSKLSAGYLQFD-CQNDGKCYSVIAVHRLSFTLVMFHLFLAFI 111 Query: 531 LISCNSLLRVVFPLVNAM 478 L CN+ RV + N + Sbjct: 112 LSLCNTRSRVAIKIQNGL 129 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,344,999 Number of Sequences: 5004 Number of extensions: 65664 Number of successful extensions: 157 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 151 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 157 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 426466470 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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