BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BmNP01_FL5_D03 (849 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 160 3e-40 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 154 2e-38 SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosacc... 57 3e-09 SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 35 0.013 SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc... 33 0.068 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 31 0.16 SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 28 1.5 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 27 2.5 SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosac... 27 3.4 SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 27 4.4 SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pom... 26 5.9 SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces... 26 5.9 SPAC1002.05c |jmj2||histone demethylase Jmj2 |Schizosaccharomyce... 26 5.9 SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm... 26 5.9 SPAC824.09c |||GTPase activating protein |Schizosaccharomyces po... 26 7.8 SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 26 7.8 >SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 160 bits (388), Expect = 3e-40 Identities = 83/207 (40%), Positives = 128/207 (61%), Gaps = 2/207 (0%) Frame = +3 Query: 147 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRV--NVIHARQQVRSPVTNIARTSFFHI 320 M +FV N KG+V+ISR YR DI + V+ F + + Q +P ++ +I Sbjct: 1 MASAIFVLNLKGKVIISRDYRADIPMSVVEKFLPLKSEVEEEQGFSTPCLTHEGINYIYI 60 Query: 321 KRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDF 500 +++L A++K N +A + FL K+ DV YF ++ EE+I++NFVL+YELLDEI+DF Sbjct: 61 HHNDVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEIMDF 120 Query: 501 GYPQNSDTGVLKTFITQQGIKSASKEEQAQITSQVTGQIGWRREGIKYRRNELFLDVLXY 680 G+PQ ++T +L+ +ITQ S + ++ A +T I WR EGI YR+NE+FLDV+ Sbjct: 121 GFPQTTETKILQEYITQ---TSNTVKKHAPPPIAMTNAISWRSEGIHYRKNEVFLDVIES 177 Query: 681 VNLLMSPQGQVLSAHVAGKVVMXSYLS 761 VNL+ + G V+ + + GKV + YLS Sbjct: 178 VNLIAAADGTVIQSEILGKVRLKCYLS 204 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 154 bits (373), Expect = 2e-38 Identities = 74/215 (34%), Positives = 130/215 (60%), Gaps = 10/215 (4%) Frame = +3 Query: 147 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHIKR 326 MI GLF++N KG+ LI + +R D+ ++ + FRV ++ R P+ +I +++ + K Sbjct: 1 MISGLFIFNLKGDTLICKTFRHDLKKSVTEIFRVAIL-TNTDYRHPIVSIGSSTYIYTKH 59 Query: 327 ANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGY 506 ++++ A+TK N N +V EFL +I + YFGK++E +K+N I+ELLDE++D+G Sbjct: 60 EDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKDNVSFIFELLDEMIDYGI 119 Query: 507 PQNSDTGVLKTFITQQGIK------SASKEEQAQI----TSQVTGQIGWRREGIKYRRNE 656 Q ++ L ++ +K S + +Q+ +S++ G + WRR GIKYR+N Sbjct: 120 IQTTEPDALARSVSITAVKKKGNALSLKRSHSSQLAHTTSSEIPGSVPWRRAGIKYRKNS 179 Query: 657 LFLDVLXYVNLLMSPQGQVLSAHVAGKVVMXSYLS 761 +++D++ +NLL+S G VL + V+G V M + LS Sbjct: 180 IYIDIVERMNLLISSTGNVLRSDVSGVVKMRAMLS 214 >SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 425 Score = 57.2 bits (132), Expect = 3e-09 Identities = 30/131 (22%), Positives = 61/131 (46%), Gaps = 3/131 (2%) Frame = +3 Query: 330 NIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYP 509 ++ L T + + + + +I+DV++++FG + ++ N +I +LL E++D+GY Sbjct: 66 DVRLCIPTTCDTEPLYIHDIMRRIVDVVKTFFGGFNASKVEKNVCVIVQLLAEMIDYGYA 125 Query: 510 QNSDTGVLKTFITQQGIKS---ASKEEQAQITSQVTGQIGWRREGIKYRRNELFLDVLXY 680 + L+ + + A Q + + WR KY NE F+ VL Sbjct: 126 TCMEPNALQDIVPLPSFMNKFMAVTGLQTNTPTLARDTVPWRTAKAKYATNEFFIHVLER 185 Query: 681 VNLLMSPQGQV 713 V+ + P G++ Sbjct: 186 VSAVYQPNGKL 196 >SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 35.1 bits (77), Expect = 0.013 Identities = 25/119 (21%), Positives = 54/119 (45%), Gaps = 3/119 (2%) Frame = +3 Query: 147 MIGGLFVYNHKGEVLISRVYR--DDIGRNAVDAFRVNVIHAR-QQVRSPVTNIARTSFFH 317 MI + + N G+ +S+ Y DD + + A +I R Q+ ++ + + Sbjct: 1 MIQFILIQNRHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVY 60 Query: 318 IKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEIL 494 + A ++ N + E + ++++ S+FG + E ++ NF + +LDEI+ Sbjct: 61 RRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEII 119 >SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 240 Score = 32.7 bits (71), Expect = 0.068 Identities = 28/140 (20%), Positives = 57/140 (40%), Gaps = 2/140 (1%) Frame = +3 Query: 165 VYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTS--FFHIKRANIW 338 + N G+ +ISR +R+ + R V++ ++ A +S T + + F + ++ Sbjct: 8 IVNRGGKAIISRQFRE-MSRVRVESL-LSSFPALVSEKSQNTTVESDNVRFVYQPLDELY 65 Query: 339 LAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQNS 518 + +T N + L + V+ S + E I I+ DE GY N Sbjct: 66 IVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLGYRDNV 125 Query: 519 DTGVLKTFITQQGIKSASKE 578 +KT++ + + +E Sbjct: 126 SLTQIKTYLEMESHEEKIQE 145 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 31.5 bits (68), Expect = 0.16 Identities = 17/55 (30%), Positives = 28/55 (50%) Frame = -1 Query: 516 NSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTGTQIPWPRSHFAL 352 +S + RS AT +S++S S + S T S+ ++ TQ+ WP +H L Sbjct: 719 DSGSRRVTRSQNATSQSQESGS--EIGSSIAGTAGSYNVGTSNTQLSWPSTHSTL 771 >SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual Length = 483 Score = 28.3 bits (60), Expect = 1.5 Identities = 12/46 (26%), Positives = 29/46 (63%) Frame = +3 Query: 315 HIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIK 452 +I+ N + + +++ + A++ E +++DV QS+ G + EEN++ Sbjct: 414 NIEITNPIIVSPVERSRSQALLNEIQTRVLDVRQSHGGVVLEENVR 459 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 27.5 bits (58), Expect = 2.5 Identities = 14/50 (28%), Positives = 20/50 (40%) Frame = -2 Query: 248 HSECVHSITTNIISVDSRDQHLAFMVINEQAPDHCG*GLTLFQNWFQCNC 99 H +C + T IS S + IN + P H + NW C+C Sbjct: 442 HKKCYPKVVTKCISKSSDSASSEYEKINHRIPHHFESHTNIGANWC-CHC 490 >SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 165 Score = 27.1 bits (57), Expect = 3.4 Identities = 21/127 (16%), Positives = 55/127 (43%), Gaps = 8/127 (6%) Frame = +3 Query: 147 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPV------TNI--AR 302 MI +F++N+KG+ +++ Y ++ + I+A R P +N+ + Sbjct: 1 MIYAVFIFNNKGKPRLTKFYTPI--DESIQQKLIGDIYAAVSTRPPTACNFLESNLIAGK 58 Query: 303 TSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQSYFGKISEENIKNNFVLIYELL 482 + + A ++ V + + + + + ++ + F + E ++ F I+ +L Sbjct: 59 NRIIYRQYATLYFVFVVDEGESELGILDLIQVFVEALDRCFNNVCELDLVFKFQEIHAIL 118 Query: 483 DEILDFG 503 E++ G Sbjct: 119 AEVVSGG 125 >SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces pombe|chr 3|||Manual Length = 1364 Score = 26.6 bits (56), Expect = 4.4 Identities = 15/47 (31%), Positives = 22/47 (46%) Frame = +3 Query: 414 QSYFGKISEENIKNNFVLIYELLDEILDFGYPQNSDTGVLKTFITQQ 554 + + KI EE +K N +L YE D +S ++KTF Q Sbjct: 952 KDFVTKIEEETLKENPLLFYEYRKASSDMRVLMDSQFLMMKTFARLQ 998 >SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 1188 Score = 26.2 bits (55), Expect = 5.9 Identities = 23/102 (22%), Positives = 48/102 (47%), Gaps = 11/102 (10%) Frame = +3 Query: 237 AFRVNVIHARQQVRSPVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIIDVMQ 416 + RVN+ + +S +N + T+ +H++ + + +A+VFEF K + Sbjct: 839 SIRVNIAGVKITAKSSTSNSSSTAEYHVRSRHAVIP--VNNRYRSAVVFEF-RKQLQRKH 895 Query: 417 SYFGKI----SEENIKNNF-VLIY------ELLDEILDFGYP 509 + F + E+N++ N V I+ +L ++DF +P Sbjct: 896 NVFAMVWLVDLEDNVEQNIRVPIFTSSKPAHVLQNMIDFDHP 937 >SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 180 Score = 26.2 bits (55), Expect = 5.9 Identities = 11/37 (29%), Positives = 23/37 (62%) Frame = +3 Query: 408 VMQSYFGKISEENIKNNFVLIYELLDEILDFGYPQNS 518 V++++ G+I+++ ++ V+IY + D L FG S Sbjct: 113 VLKAHVGRITDDTPQHQGVVIYSMNDTPLGFGVTARS 149 >SPAC1002.05c |jmj2||histone demethylase Jmj2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 715 Score = 26.2 bits (55), Expect = 5.9 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = -2 Query: 353 CDCCQPNICTLDMEERCACNVGNR 282 C C +C L +E C C++ N+ Sbjct: 675 CSCKSITVCPLHIEYLCKCDLSNK 698 >SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1292 Score = 26.2 bits (55), Expect = 5.9 Identities = 17/54 (31%), Positives = 24/54 (44%) Frame = -1 Query: 537 SSGPQYQNSEGNQSPRSHQATHRSEQSYS*CSLQKSCQNTIASHQ*SSTGTQIP 376 SS P +SE NQ P S Q +Y+ ++ S + SS+GT P Sbjct: 10 SSRPPSVHSERNQKPSSSQFLGVPSSNYNQRENSSRSGSSTISREPSSSGTMYP 63 >SPAC824.09c |||GTPase activating protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 25.8 bits (54), Expect = 7.8 Identities = 11/34 (32%), Positives = 18/34 (52%) Frame = +1 Query: 271 RCDHLLPTLHAHLSSISSVQIFGWQQSQSKM*TR 372 RC + +L H+S + SV + W Q++ TR Sbjct: 51 RCSGVHRSLGVHVSRVKSVDLDSWTDEQTENMTR 84 >SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 878 Score = 25.8 bits (54), Expect = 7.8 Identities = 16/58 (27%), Positives = 28/58 (48%) Frame = +3 Query: 63 YVQFLKGCNR*IAITLKPILKECEASPTMIGGLFVYNHKGEVLISRVYRDDIGRNAVD 236 +V ++ G R I + L P+L PT+ +NH G++ + R + G N+ D Sbjct: 737 HVSYVTGVIRSIDLQL-PVLLSKFMKPTIFDSYDFFNHWGQMGVEREAQLTFGLNSKD 793 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,400,201 Number of Sequences: 5004 Number of extensions: 69668 Number of successful extensions: 188 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 180 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 183 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 420459900 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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