BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BmNP01_FL5_B19 (871 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB8E5.06c |rpl302|rpl3-2, rpl3-b|60S ribosomal protein L3|Sch... 131 1e-31 SPAC17A5.03 |rpl301|rpl3-1, rpl3|60S ribosomal protein L3 |Schiz... 130 2e-31 SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 28 1.5 SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 27 3.5 SPBC1685.07c |||amino acid transporter |Schizosaccharomyces pomb... 26 8.0 >SPAPB8E5.06c |rpl302|rpl3-2, rpl3-b|60S ribosomal protein L3|Schizosaccharomyces pombe|chr 1|||Manual Length = 388 Score = 131 bits (316), Expect = 1e-31 Identities = 57/73 (78%), Positives = 68/73 (93%) Frame = +3 Query: 180 YKAGMTHVVREPDRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAE 359 YKAGMTH+VR+ DRPGSK++K+EI+EAVTIIETPPMV VGVVGY+ETP GLR+L TVWAE Sbjct: 49 YKAGMTHIVRDLDRPGSKMHKREILEAVTIIETPPMVVVGVVGYVETPRGLRSLTTVWAE 108 Query: 360 HMSEDCRRRFYKN 398 H+SE+ +RRFYKN Sbjct: 109 HLSEEVKRRFYKN 121 Score = 74.9 bits (176), Expect = 1e-14 Identities = 31/42 (73%), Positives = 36/42 (85%) Frame = +2 Query: 50 FQHPXHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFI 175 F+ P HGS+GF P+KR+ R RGKVKAFPKDD SKPVHLTAF+ Sbjct: 6 FEQPRHGSLGFLPRKRASRQRGKVKAFPKDDASKPVHLTAFL 47 >SPAC17A5.03 |rpl301|rpl3-1, rpl3|60S ribosomal protein L3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 388 Score = 130 bits (315), Expect = 2e-31 Identities = 56/73 (76%), Positives = 68/73 (93%) Frame = +3 Query: 180 YKAGMTHVVREPDRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAE 359 YKAGMTH+VR+ DRPGSK++K+EI+EAVT+IETPPMV VGVVGY+ETP GLR+L TVWAE Sbjct: 49 YKAGMTHIVRDLDRPGSKMHKREILEAVTVIETPPMVVVGVVGYVETPRGLRSLTTVWAE 108 Query: 360 HMSEDCRRRFYKN 398 H+SE+ +RRFYKN Sbjct: 109 HLSEEVKRRFYKN 121 Score = 74.9 bits (176), Expect = 1e-14 Identities = 31/42 (73%), Positives = 36/42 (85%) Frame = +2 Query: 50 FQHPXHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFI 175 F+ P HGS+GF P+KR+ R RGKVKAFPKDD SKPVHLTAF+ Sbjct: 6 FEQPRHGSLGFLPRKRASRQRGKVKAFPKDDASKPVHLTAFL 47 >SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharomyces pombe|chr 1|||Manual Length = 706 Score = 28.3 bits (60), Expect = 1.5 Identities = 11/46 (23%), Positives = 25/46 (54%) Frame = -2 Query: 501 PSSLIVERRLLGNYMHKVTYSRHINKALIFREESSFCRSDVDSLQT 364 P++ + ++LGNY+ +T+ ++ A F E +C+ + + T Sbjct: 580 PTTSVQPSQILGNYVTGITFMNKVDYATYFSAE-EYCKQGIHMIPT 624 >SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosaccharomyces pombe|chr 2|||Manual Length = 658 Score = 27.1 bits (57), Expect = 3.5 Identities = 12/42 (28%), Positives = 20/42 (47%), Gaps = 2/42 (4%) Frame = +2 Query: 38 RTENFQHPXH--GSMGFYPKKRSRRHRGKVKAFPKDDPSKPV 157 ++E QHP +M FY + + G FP++ KP+ Sbjct: 186 KSEQVQHPQAVLDAMAFYSQSKKYLEEGAKPPFPRESTEKPL 227 >SPBC1685.07c |||amino acid transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 420 Score = 25.8 bits (54), Expect = 8.0 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = -3 Query: 470 LVTICTK*HTLGILIKLSSFERRAVFVEATSTVFRHMLRPDS 345 ++T+C T + + LSS E FV +T + F + P S Sbjct: 328 VITLCILLFTHSLALLLSSLEMVLAFVGSTGSTFISFILPGS 369 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,974,062 Number of Sequences: 5004 Number of extensions: 55850 Number of successful extensions: 125 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 121 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 125 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 434475230 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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