BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BmNP01_FL5_B09 (856 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholi... 69 4e-14 AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic ac... 69 4e-14 DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholi... 65 7e-13 AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic ac... 65 9e-13 DQ026037-1|AAY87896.1| 431|Apis mellifera nicotinic acetylcholi... 64 1e-12 DQ026036-1|AAY87895.1| 529|Apis mellifera nicotinic acetylcholi... 64 1e-12 DQ026035-1|AAY87894.1| 529|Apis mellifera nicotinic acetylcholi... 64 1e-12 EF127800-1|ABL67937.1| 461|Apis mellifera nicotinic acetylcholi... 64 2e-12 DQ667183-1|ABG75735.1| 463|Apis mellifera GABA-gated ion channe... 64 2e-12 DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 63 3e-12 EF127801-1|ABL67938.1| 461|Apis mellifera nicotinic acetylcholi... 63 4e-12 EF127805-1|ABL67942.1| 461|Apis mellifera nicotinic acetylcholi... 62 5e-12 AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic ac... 62 5e-12 EF127803-1|ABL67940.1| 461|Apis mellifera nicotinic acetylcholi... 62 8e-12 EF127804-1|ABL67941.1| 461|Apis mellifera nicotinic acetylcholi... 61 1e-11 EF127802-1|ABL67939.1| 461|Apis mellifera nicotinic acetylcholi... 61 1e-11 DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholi... 61 1e-11 DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 61 1e-11 DQ026039-1|AAY87898.1| 427|Apis mellifera nicotinic acetylcholi... 57 2e-10 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 54 2e-09 DQ667185-1|ABG75737.1| 447|Apis mellifera glutamate-gated chlor... 54 2e-09 DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated... 52 5e-09 DQ667186-1|ABG75738.1| 447|Apis mellifera glutamate-gated chlor... 51 2e-08 DQ667182-1|ABG75734.1| 445|Apis mellifera GABA-gated chloride c... 46 3e-07 DQ667181-1|ABG75733.1| 445|Apis mellifera GABA-gated chloride c... 45 8e-07 DQ667184-1|ABG75736.1| 489|Apis mellifera GABA-gated ion channe... 42 1e-05 DQ667192-1|ABG75744.1| 489|Apis mellifera pH-sensitive chloride... 39 7e-05 DQ667191-1|ABG75743.1| 475|Apis mellifera pH-sensitive chloride... 39 7e-05 DQ667189-1|ABG75741.1| 458|Apis mellifera pH-sensitive chloride... 39 7e-05 DQ667188-1|ABG75740.1| 383|Apis mellifera histamine-gated chlor... 38 9e-05 AF094822-1|AAC63381.1| 365|Apis mellifera GABA receptor Rdl sub... 38 2e-04 DQ667187-1|ABG75739.1| 428|Apis mellifera histamine-gated chlor... 36 5e-04 DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride... 34 0.002 DQ667195-1|ABG75747.1| 469|Apis mellifera cys-loop ligand-gated... 31 0.014 DQ667194-1|ABG75746.1| 391|Apis mellifera cys-loop ligand-gated... 28 0.095 >DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholine receptor beta1subunit protein. Length = 520 Score = 69.3 bits (162), Expect = 4e-14 Identities = 42/159 (26%), Positives = 79/159 (49%), Gaps = 11/159 (6%) Frame = +3 Query: 192 KIVVNLTL-HLRHANIRESESTVRILADLQMNWIDKRLSWNAGEWG-YSTWLVSSERLWR 365 K+ VN L ++ N+ E ++ L+ W D +L W+ ++G + +++W+ Sbjct: 51 KVHVNFGLAFVQLINVNEKNQIMKSNVWLRFIWTDYQLQWDEADYGGIGVLRLPPDKVWK 110 Query: 366 PDVVLLNAAATTAGDYALRAR----VSNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTF 533 PD+VL N A G+Y +R + + NG V W+ + ++ + +P D QTC Sbjct: 111 PDIVLFNNAD---GNYEVRYKSNVLIYPNGDVLWVPPAIYQSSCTIDVTYFPFDQQTCIM 167 Query: 534 KFGSRMHNSDEMDFVI--DKRIYSM---FESGAWDVTDL 635 KFGS N D++ + +K + ++SG WD+ ++ Sbjct: 168 KFGSWTFNGDQVSLALYNNKNFVDLSDYWKSGTWDIINV 206 >AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic acetylcholine receptorApisa2 subunit protein. Length = 541 Score = 69.3 bits (162), Expect = 4e-14 Identities = 51/168 (30%), Positives = 76/168 (45%), Gaps = 9/168 (5%) Frame = +3 Query: 96 IASAAECENATSLSLMIDSLLATYDRESPPDSK----IVVNLTLHLRHA---NIRESEST 254 I SA C + D LL+ Y+R P S +VV L L L N+++ T Sbjct: 9 ILSARICHGNPDAKRLYDDLLSNYNRLIRPVSNNNDTVVVKLGLRLSQLIDLNLKDQILT 68 Query: 255 VRILADLQMNWIDKRLSWNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTAGDYAL-RAR 428 + L+ W D + W+ E+G T L V SE +W PD+VL N A G + +A Sbjct: 69 TNVW--LEHEWQDHKFQWDPAEYGGVTELYVPSEHIWLPDIVLYNNADGEYGVTTMTKAI 126 Query: 429 VSNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMD 572 + G V W + + + +P D QTC KFGS ++ ++D Sbjct: 127 LHYTGKVLWTPPAIFKSSCEIDVRYFPFDQQTCFMKFGSWTYDGIQID 174 >DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholine receptor alpha3subunit protein. Length = 566 Score = 65.3 bits (152), Expect = 7e-13 Identities = 42/162 (25%), Positives = 74/162 (45%), Gaps = 9/162 (5%) Frame = +3 Query: 114 CENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLR-------HANIRESESTVRILAD 272 C + D LL+ Y++ P + LT+ ++ N++ T + Sbjct: 19 CSGNPDAKRLYDDLLSNYNKLVRPVVNVTDALTVKIKLKLSQLIDVNLKNQIMTTNLW-- 76 Query: 273 LQMNWIDKRLSWNAGEWGYSTWL-VSSERLWRPDVVLLNAA-ATTAGDYALRARVSNNGS 446 ++ +W D +L W+ E+G L V S+ +WRPD+VL N A A +A ++ G Sbjct: 77 VEQSWYDYKLKWDPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGR 136 Query: 447 VSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMD 572 V W + + ++ +P D QTC KFGS ++ ++D Sbjct: 137 VEWKPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVD 178 >AF514804-1|AAM51823.1| 537|Apis mellifera neuronal nicotinic acetylcholine receptoralpha-3 protein. Length = 537 Score = 64.9 bits (151), Expect = 9e-13 Identities = 46/154 (29%), Positives = 74/154 (48%), Gaps = 10/154 (6%) Frame = +3 Query: 141 MIDSLLATYDRESPPDSKIVVNLT--LHLRHANIRESESTVRILAD---LQMNWIDKRLS 305 + D LL+ Y+R P LT L L+ + + E +++ ++ W D +L Sbjct: 37 LYDDLLSNYNRLIRPVMNNTETLTVQLGLKLSQLIEMNLKNQVMTTNVWVEQRWNDYKLK 96 Query: 306 WNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTAGDYAL----RARVSNNGSVSWIKRLD 470 WN E+G L V SE +W PD+VL N A G+Y + +A + G VSW Sbjct: 97 WNPEEYGGVEMLYVPSENIWLPDIVLYNNAD---GNYEVTLMTKATLKYTGDVSWKPPAI 153 Query: 471 ISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMD 572 + + ++ +P D Q+C KFGS +N ++D Sbjct: 154 YKSSCEINVEYFPFDEQSCIMKFGSWTYNGAQVD 187 >DQ026037-1|AAY87896.1| 431|Apis mellifera nicotinic acetylcholine receptor alpha9subunit protein. Length = 431 Score = 64.5 bits (150), Expect = 1e-12 Identities = 43/170 (25%), Positives = 79/170 (46%), Gaps = 8/170 (4%) Frame = +3 Query: 117 ENATSLSLMIDSLLATYDRESPPDS--KIVVNLTLHL--RHANIRESESTVRILADLQMN 284 ++ T+L + L YD P S +I N+T+ L + + S + + + + + Sbjct: 39 DHPTTLLKLKRYLFCEYDPNVRPISSHQIANNVTMQLLPKLMEFDDWTSVMELHSWMTLM 98 Query: 285 WIDKRLSWNAGEW-GYSTWLVSSERLWRPDVVLLNAAATT---AGDYALRARVSNNGSVS 452 W D LSW ++ G + V S+ +W PD+ + N+ T G V ++GSVS Sbjct: 99 WTDSHLSWKPSDFDGINYIYVKSDDIWVPDISVYNSGDMTFDQTGIPPTTCLVFSSGSVS 158 Query: 453 WIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFVIDKRIYSM 602 + + + +WP D C FGS +H+ +E++ +DK+ + M Sbjct: 159 CVPSVKHVAKCATDFSSWPYDTHRCRINFGSWVHSGEEVNIFLDKKGFHM 208 >DQ026036-1|AAY87895.1| 529|Apis mellifera nicotinic acetylcholine receptor alpha6subunit protein. Length = 529 Score = 64.5 bits (150), Expect = 1e-12 Identities = 39/154 (25%), Positives = 77/154 (50%), Gaps = 7/154 (4%) Frame = +3 Query: 141 MIDSLLATYDRESPP----DSKIVVNLTLHLRHA-NIRESESTVRILADLQMNWIDKRLS 305 +++ LL++Y+ P + V + L+ ++ E + A L++ W+D L Sbjct: 58 LLNELLSSYNTLERPVANESEPLEVKFGITLQQIIDVDEKNQILTTNAWLKLEWVDYNLQ 117 Query: 306 WNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTA-GDYALRARVSNNGSVSWIKRLDIST 479 WN E+G L ++ +LW+PD+++ N+A G Y V++NGS ++ + Sbjct: 118 WNESEYGGVKDLRITPNKLWKPDILMYNSADEGFDGTYQTNVVVTHNGSCLYVPPGIFKS 177 Query: 480 PISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFVI 581 + + +P D Q C KFGS ++ +++D V+ Sbjct: 178 TCKIDITWFPFDDQHCDMKFGSWTYDGNQVDLVL 211 >DQ026035-1|AAY87894.1| 529|Apis mellifera nicotinic acetylcholine receptor alpha6subunit protein. Length = 529 Score = 64.5 bits (150), Expect = 1e-12 Identities = 39/154 (25%), Positives = 77/154 (50%), Gaps = 7/154 (4%) Frame = +3 Query: 141 MIDSLLATYDRESPP----DSKIVVNLTLHLRHA-NIRESESTVRILADLQMNWIDKRLS 305 +++ LL++Y+ P + V + L+ ++ E + A L++ W+D L Sbjct: 58 LLNELLSSYNTLERPVANESEPLEVKFGITLQQIIDVDEKNQILTTNAWLKLEWVDYNLQ 117 Query: 306 WNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTA-GDYALRARVSNNGSVSWIKRLDIST 479 WN E+G L ++ +LW+PD+++ N+A G Y V++NGS ++ + Sbjct: 118 WNESEYGGVKDLRITPNKLWKPDILMYNSADEGFDGTYQTNVVVTHNGSCLYVPPGIFKS 177 Query: 480 PISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFVI 581 + + +P D Q C KFGS ++ +++D V+ Sbjct: 178 TCKIDITWFPFDDQHCDMKFGSWTYDGNQVDLVL 211 >EF127800-1|ABL67937.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 1 protein. Length = 461 Score = 63.7 bits (148), Expect = 2e-12 Identities = 33/119 (27%), Positives = 62/119 (52%), Gaps = 2/119 (1%) Frame = +3 Query: 231 NIRESESTVRILADLQMNWIDKRLSWNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTA- 404 ++ E + A L++ W+D L WN E+G L ++ +LW+PD+++ N+A Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYGGVKDLRITPNKLWKPDILMYNSADEGFD 84 Query: 405 GDYALRARVSNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFVI 581 G Y V++NGS ++ + + + +P D Q C KFGS ++ +++D V+ Sbjct: 85 GTYQTSVVVTHNGSCLYVPPGIFKSTCKIDITWFPFDDQHCDMKFGSWTYDGNQVDLVL 143 >DQ667183-1|ABG75735.1| 463|Apis mellifera GABA-gated ion channel protein. Length = 463 Score = 63.7 bits (148), Expect = 2e-12 Identities = 41/149 (27%), Positives = 70/149 (46%), Gaps = 7/149 (4%) Frame = +3 Query: 120 NATSLSLMIDSLLATYDRESPPD---SKIVVNLTLHLRHAN-IRESESTVRILADLQMNW 287 N +++S ++D+LL YD PD V + + +R I E + T + + +W Sbjct: 2 NHSNISELLDNLLRGYDNSVRPDFGGPPATVEVDIMVRSMGPISEVDMTYSMDCYFRQSW 61 Query: 288 IDKRLSWNAGEWGYSTWLVSSERLWRPDVVLLNAAAT---TAGDYALRARVSNNGSVSWI 458 +D+RL++ G+ + + R+W+PD N + T R+ +G V + Sbjct: 62 VDRRLAFQGGKETLALSISMLARIWKPDTYFYNGKHSYLHTITSPNKFVRLYQDGRVLYS 121 Query: 459 KRLDISTPISMQLDNWPNDMQTCTFKFGS 545 RL I M L+N+P D Q C +FGS Sbjct: 122 SRLTIKAGCPMNLENFPMDTQRCPLQFGS 150 >DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 63.3 bits (147), Expect = 3e-12 Identities = 40/151 (26%), Positives = 75/151 (49%), Gaps = 7/151 (4%) Frame = +3 Query: 141 MIDSLLATYDRESPP--DSKIVVNLTLHLRHANIRESESTVRILAD---LQMNWIDKRLS 305 + D LL+ Y++ P ++ V+ + + L+ + + + +I+ ++ +W D +L Sbjct: 32 LYDDLLSNYNKLVRPVVNTSDVLRVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLR 91 Query: 306 WNAGEWGYSTWL-VSSERLWRPDVVLLNAA-ATTAGDYALRARVSNNGSVSWIKRLDIST 479 W E+G L V S+ +WRPD+VL N A A +A + + G V W + Sbjct: 92 WEPKEYGGVKMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATIYHQGLVEWKPPAIYKS 151 Query: 480 PISMQLDNWPNDMQTCTFKFGSRMHNSDEMD 572 + ++ +P D QTC KFGS ++ ++D Sbjct: 152 SCEIDVEYFPFDEQTCVLKFGSWTYDGFKVD 182 >EF127801-1|ABL67938.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 2 protein. Length = 461 Score = 62.9 bits (146), Expect = 4e-12 Identities = 33/119 (27%), Positives = 62/119 (52%), Gaps = 2/119 (1%) Frame = +3 Query: 231 NIRESESTVRILADLQMNWIDKRLSWNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTA- 404 ++ E + A L++ W+D L WN E+G L ++ +LW+PD+++ N+A Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYGGVKDLRITPNKLWKPDILMYNSADEGFD 84 Query: 405 GDYALRARVSNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFVI 581 G Y V+++GS ++ + M + +P D Q C KFGS ++ +++D V+ Sbjct: 85 GTYQTSVVVTHDGSCLYVPPGIFKSTCKMDIAWFPFDDQHCDMKFGSWTYDGNQVDLVL 143 >EF127805-1|ABL67942.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 6 protein. Length = 461 Score = 62.5 bits (145), Expect = 5e-12 Identities = 33/119 (27%), Positives = 62/119 (52%), Gaps = 2/119 (1%) Frame = +3 Query: 231 NIRESESTVRILADLQMNWIDKRLSWNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTA- 404 ++ E + A L++ W+D L WN E+G L ++ +LW+PD+++ N+A Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYGGVKDLRITPNKLWKPDILMYNSADEGFD 84 Query: 405 GDYALRARVSNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFVI 581 G Y V+++GS ++ + M + +P D Q C KFGS ++ +++D V+ Sbjct: 85 GTYQTSVVVTHDGSCLYVPPGIFKSTCKMDVAWFPFDDQHCDMKFGSWTYDGNQVDLVL 143 >AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic acetylcholine receptoralpha7-1 protein. Length = 555 Score = 62.5 bits (145), Expect = 5e-12 Identities = 47/189 (24%), Positives = 87/189 (46%), Gaps = 11/189 (5%) Frame = +3 Query: 93 AIASAAECENATSLSLMIDSLLATYD-RESPPDSK---IVVNLTLHLRHA-NIRESESTV 257 A+A++ + +++ LL TY+ E P ++ +V++ L L ++ E + Sbjct: 11 ALAASGLVNGGSHEKRLLNDLLDTYNVLERPVGNESEPLVLSFGLTLMQIIDVDEKNQLL 70 Query: 258 RILADLQMNWIDKRLSWNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTA-GDYALRARV 431 L++ W D + WN ++G L + RLW+PDV++ N+A G Y V Sbjct: 71 ITNLWLKLEWNDVNMRWNVSDYGGVRDLRIPPHRLWKPDVLMYNSADEGFDGTYPTNVVV 130 Query: 432 SNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFVIDKR----IYS 599 NNG+ ++ + + + +P D Q C KFGS ++ ++D + I S Sbjct: 131 KNNGTCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDEAGGDISS 190 Query: 600 MFESGAWDV 626 +G WD+ Sbjct: 191 FITNGEWDL 199 >EF127803-1|ABL67940.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 4 protein. Length = 461 Score = 61.7 bits (143), Expect = 8e-12 Identities = 32/119 (26%), Positives = 62/119 (52%), Gaps = 2/119 (1%) Frame = +3 Query: 231 NIRESESTVRILADLQMNWIDKRLSWNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTA- 404 ++ E + A L++ W+D L WN E+G L ++ +LW+PD+++ N+A Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYGGVKDLRITPNKLWKPDILMYNSADEGFD 84 Query: 405 GDYALRARVSNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFVI 581 G Y V+++GS ++ + + + +P D Q C KFGS ++ +++D V+ Sbjct: 85 GTYQTSVVVTHDGSCLYVPPGIFKSTCKIDITWFPFDDQHCDMKFGSWTYDGNQVDLVL 143 >EF127804-1|ABL67941.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 5 protein. Length = 461 Score = 61.3 bits (142), Expect = 1e-11 Identities = 32/119 (26%), Positives = 62/119 (52%), Gaps = 2/119 (1%) Frame = +3 Query: 231 NIRESESTVRILADLQMNWIDKRLSWNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTA- 404 ++ E + A L++ W+D L WN E+G L ++ +LW+PD+++ N+A Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYGGVKDLRITPNKLWKPDILMYNSADEGFD 84 Query: 405 GDYALRARVSNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFVI 581 G Y V+++GS ++ + + + +P D Q C KFGS ++ +++D V+ Sbjct: 85 GTYQTSVVVTHDGSCLYVPPGIFKSTCKIDIAWFPFDDQHCDMKFGSWTYDGNQVDLVL 143 >EF127802-1|ABL67939.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 3 protein. Length = 461 Score = 61.3 bits (142), Expect = 1e-11 Identities = 32/119 (26%), Positives = 62/119 (52%), Gaps = 2/119 (1%) Frame = +3 Query: 231 NIRESESTVRILADLQMNWIDKRLSWNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTA- 404 ++ E + A L++ W+D L WN E+G L ++ +LW+PD+++ N+A Sbjct: 25 DVDEKNQILTTNAWLKLEWVDYNLQWNESEYGGVKDLRITPNKLWKPDILMYNSADEGFD 84 Query: 405 GDYALRARVSNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFVI 581 G Y V+++GS ++ + + + +P D Q C KFGS ++ +++D V+ Sbjct: 85 GTYQTSVVVTHDGSCLYVPPGIFGSTCKIDIAWFPFDDQHCDMKFGSWTYDGNQVDLVL 143 >DQ026031-1|AAY87890.1| 601|Apis mellifera nicotinic acetylcholine receptor alpha1subunit protein. Length = 601 Score = 61.3 bits (142), Expect = 1e-11 Identities = 46/174 (26%), Positives = 78/174 (44%), Gaps = 12/174 (6%) Frame = +3 Query: 87 TCAIASAAECENATSLSLMIDSLLATYDRESPP----DSKIVVNLTLHLRH---ANIRES 245 +C +A + + D LL+ Y+R P ++ V + L L N++ Sbjct: 6 SCLVAPFPGASANSEAKRLYDDLLSNYNRLIRPVGNNSDRLTVKMGLRLSQLIDVNLKNQ 65 Query: 246 ESTVRILADLQMNWIDKRLSWNAGEWG-YSTWLVSSERLWRPDVVLLNAAATTAGDYAL- 419 T + ++ W D +L WN ++G T V SE +W PD+VL N A G+Y + Sbjct: 66 IMTTNVW--VEQEWNDYKLKWNPDDYGGVDTLHVPSEHIWLPDIVLYNNA---DGNYEVT 120 Query: 420 ---RARVSNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMD 572 +A + + G V W + + ++ +P D QTC KFGS ++ +D Sbjct: 121 IMTKAILHHTGKVVWKPPAIYKSFCEIDVEYFPFDEQTCFMKFGSWTYDGYTVD 174 >DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 60.9 bits (141), Expect = 1e-11 Identities = 42/154 (27%), Positives = 79/154 (51%), Gaps = 10/154 (6%) Frame = +3 Query: 141 MIDSLLATYDRESPP--DSKIVVNLTLHLRHANIRESESTVRILAD---LQMNWIDKRLS 305 + D LL+ Y++ P ++ V+ + + L+ + + + +I+ ++ +W D +L Sbjct: 32 LYDDLLSNYNKLVRPVVNTSDVLRVCIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLR 91 Query: 306 WNAGEWGYSTWL-VSSERLWRPDVVLLNAAATTAGDYAL----RARVSNNGSVSWIKRLD 470 W E+G L V S+ +WRPD+VL N A G+Y + +A V +G V W Sbjct: 92 WEPKEYGGVKMLHVPSDHIWRPDIVLYNNAD---GNYEVTLMTKATVYYSGLVVWQPPAV 148 Query: 471 ISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMD 572 + S+ ++ +P D+QTC K GS ++ ++D Sbjct: 149 YKSSCSIDVEFFPYDVQTCVLKLGSWTYDGFKVD 182 >DQ026039-1|AAY87898.1| 427|Apis mellifera nicotinic acetylcholine receptor beta2subunit protein. Length = 427 Score = 57.2 bits (132), Expect = 2e-10 Identities = 42/162 (25%), Positives = 76/162 (46%), Gaps = 10/162 (6%) Frame = +3 Query: 114 CENATSLSLMIDS---LLATYDRESPPDSKIV--VNLTLHLRHANIRESESTVRILADLQ 278 C++ TS S + L YDR+ P+ K ++ L ++H N+ E TV L+ Sbjct: 28 CKDITSTSALYRLKLYLFCDYDRDIIPEQKNATKIDFGLSIQHYNVDEYSHTVDFHVMLK 87 Query: 279 MNWIDKRLSWNAGEW-GYSTWLVSSERLWRPDVVL--LNAAATTAGDYALRARVSNNGSV 449 + W L+W + E+ ++ V S +W PD+V+ + + ++ V N+G++ Sbjct: 88 LMWEQSHLTWKSSEFDSINSIRVKSYEIWVPDIVMHSVTSVGIDLEMPSVECIVFNSGTI 147 Query: 450 SWIKRLDISTPISMQLDN--WPNDMQTCTFKFGSRMHNSDEM 569 + TP+ + D+ WP D+ CT S H S+E+ Sbjct: 148 LCVP-FTTYTPV-CEYDHTWWPYDILNCTIHIASWSHGSNEI 187 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 54.0 bits (124), Expect = 2e-09 Identities = 33/129 (25%), Positives = 56/129 (43%), Gaps = 3/129 (2%) Frame = +3 Query: 195 IVVNLTLHLRHANIRESESTVRILADLQMNWIDKRLSWNAGEW-GYSTWLVSSERLWRPD 371 +V L+LH ++ E + + W D L WNA E+ G V R+WRPD Sbjct: 33 VVFGLSLH-HIIDVDEKNQILTTNCWVTQIWTDHHLKWNASEFAGIRVIRVPYNRVWRPD 91 Query: 372 VVLLNAAATTAGDYALRARV--SNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGS 545 +L N A + V S+ G V W+ + + ++ +P D Q C K+ S Sbjct: 92 TILYNNADPQYSSAVINTNVIVSHTGEVVWLSHGIFRSSCDIDVEFFPFDEQRCVLKWAS 151 Query: 546 RMHNSDEMD 572 ++ +++ Sbjct: 152 WTYDGYQLE 160 >DQ667185-1|ABG75737.1| 447|Apis mellifera glutamate-gated chloride channel protein. Length = 447 Score = 53.6 bits (123), Expect = 2e-09 Identities = 40/158 (25%), Positives = 72/158 (45%), Gaps = 12/158 (7%) Frame = +3 Query: 141 MIDSLLATYDR------ESPPDSKIVVNLTLHLRH-ANIRESESTVRILADLQMNWIDKR 299 ++D++L YD E+ D +V + L +R A I + + + + W+D+R Sbjct: 35 VLDNILGGYDARIRPSGENATDGPAIVRVNLFVRSIATISDIKMEYSVQLTFREQWLDER 94 Query: 300 LSWN--AGEWGYSTWLVSSERLWRPDVVLLNAAATTAGDYALR---ARVSNNGSVSWIKR 464 L +N G Y T L + R+W PD+ N + + R+ NGSV + R Sbjct: 95 LRFNDFGGRLKYLT-LTDASRVWMPDLFFSNEKEGHFHNIIMPNVYIRIFPNGSVLYSIR 153 Query: 465 LDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFV 578 + ++ M L +P D Q C+ + S +D++ F+ Sbjct: 154 ISLTLSCPMNLKLYPLDRQVCSLRMASYGWTTDDLVFL 191 >DQ667193-1|ABG75745.1| 510|Apis mellifera cys-loop ligand-gated ion channel subunit protein. Length = 510 Score = 52.4 bits (120), Expect = 5e-09 Identities = 36/147 (24%), Positives = 65/147 (44%), Gaps = 8/147 (5%) Frame = +3 Query: 129 SLSLMIDSLLATYDRESPPD----SKIVVNLTLHLRHAN-IRESESTVRILADLQMNWID 293 ++++++++LL Y+ P + VV + +R + E + + + +W D Sbjct: 36 NITMVLENLLMNYENNQLPTHGKGTPTVVKTNILIRSMGPVSELDMDYSMDCYFRQSWRD 95 Query: 294 KRLSWNAGEWGYSTWLVSSERLWRPDVVLLNAAATTAGDYALR---ARVSNNGSVSWIKR 464 RLS+ S + ER+WRPD N + + R+S +G + + R Sbjct: 96 SRLSFLGPIKSLSLSIKMLERIWRPDTYFYNGKHSYVHTITVPNKLLRISQDGDILYSMR 155 Query: 465 LDISTPISMQLDNWPNDMQTCTFKFGS 545 L I M+L N+P D Q+C GS Sbjct: 156 LTIKAKCPMELRNFPMDRQSCPLILGS 182 >DQ667186-1|ABG75738.1| 447|Apis mellifera glutamate-gated chloride channel protein. Length = 447 Score = 50.8 bits (116), Expect = 2e-08 Identities = 39/158 (24%), Positives = 71/158 (44%), Gaps = 12/158 (7%) Frame = +3 Query: 141 MIDSLLATYDR------ESPPDSKIVVNLTLHLRH-ANIRESESTVRILADLQMNWIDKR 299 ++D++L YD E+ D VV + + +R + I + + + W+D+R Sbjct: 35 VLDNILGGYDARIRPSGENATDGPAVVRVNIFVRSISKIDDVTMEYSVQLTFREQWLDER 94 Query: 300 LSWN--AGEWGYSTWLVSSERLWRPDVVLLNAAATTAGDYALR---ARVSNNGSVSWIKR 464 L +N G Y T L + R+W PD+ N + + R+ NGSV + R Sbjct: 95 LRFNDFGGRLKYLT-LTDASRVWMPDLFFSNEKEGHFHNIIMPNVYIRIFPNGSVLYSIR 153 Query: 465 LDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFV 578 + ++ M L +P D Q C+ + S +D++ F+ Sbjct: 154 ISLTLSCPMNLKLYPLDRQVCSLRMASYGWTTDDLVFL 191 >DQ667182-1|ABG75734.1| 445|Apis mellifera GABA-gated chloride channel protein. Length = 445 Score = 46.4 bits (105), Expect = 3e-07 Identities = 40/155 (25%), Positives = 72/155 (46%), Gaps = 13/155 (8%) Frame = +3 Query: 120 NATSLSLMIDSLLATYDRESPPD---SKIVVNLTLH-LRHANIRESESTVRILADLQMNW 287 N ++S ++DS +YD+ P+ + V +T++ L +++ E + + + W Sbjct: 8 NDVNISAILDSFSVSYDKRVRPNYGGPPVEVGVTMYVLSISSLSEVKMDFTLDFYFRQFW 67 Query: 288 IDKRLSWNAGEWGYSTWLVSSE---RLWRPDVVLLNAA------ATTAGDYALRARVSNN 440 D RL++ G T V SE +W PD +N ATT+ ++ R+ ++ Sbjct: 68 TDPRLAFKK-RTGVETLSVGSEFIKNIWVPDTFFVNEKQSYFHIATTSNEFI---RIHHS 123 Query: 441 GSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGS 545 GS++ RL I+ M L +P D Q C + S Sbjct: 124 GSITRSIRLTITASCPMNLQYFPMDRQLCHIEIES 158 >DQ667181-1|ABG75733.1| 445|Apis mellifera GABA-gated chloride channel protein. Length = 445 Score = 45.2 bits (102), Expect = 8e-07 Identities = 40/155 (25%), Positives = 71/155 (45%), Gaps = 13/155 (8%) Frame = +3 Query: 120 NATSLSLMIDSLLATYDRESPPD---SKIVVNLTLH-LRHANIRESESTVRILADLQMNW 287 N ++S ++DS +YD+ P+ + V +T++ L +++ E + + W Sbjct: 8 NDVNISAILDSFSVSYDKRVRPNYGGPPVEVGVTMYVLSISSVSEVLMDFTLDFYFRQFW 67 Query: 288 IDKRLSWNAGEWGYSTWLVSSE---RLWRPDVVLLNAA------ATTAGDYALRARVSNN 440 D RL++ G T V SE +W PD +N ATT+ ++ R+ ++ Sbjct: 68 TDPRLAFKK-RTGVETLSVGSEFIKNIWVPDTFFVNEKQSYFHIATTSNEFI---RIHHS 123 Query: 441 GSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGS 545 GS++ RL I+ M L +P D Q C + S Sbjct: 124 GSITRSIRLTITASCPMNLQYFPMDRQLCHIEIES 158 >DQ667184-1|ABG75736.1| 489|Apis mellifera GABA-gated ion channel protein. Length = 489 Score = 41.5 bits (93), Expect = 1e-05 Identities = 37/141 (26%), Positives = 60/141 (42%), Gaps = 7/141 (4%) Frame = +3 Query: 144 IDSLLATYDRESPPD---SKIVVNLTLHLRHAN-IRESESTVRILADLQMNWIDKRLSWN 311 I +L YD P+ ++V + L + + I E I L W D+RL+++ Sbjct: 41 ISRILDGYDIRLRPNFGGEPLLVGMDLTIASFDAISEVNMDYTITMYLNQYWKDERLAFS 100 Query: 312 AGEWGYSTWLVSSERLWRPDVVLLNAAATTAGDYALR---ARVSNNGSVSWIKRLDISTP 482 E + +E++W PD N + D R R+S +GSV++ R + Sbjct: 101 QEEEVLTLSGDFAEKIWVPDTFFANDKNSFLHDVTERNKLVRLSGDGSVTYGMRFTTTLA 160 Query: 483 ISMQLDNWPNDMQTCTFKFGS 545 M L +P D Q CT + S Sbjct: 161 CMMDLHYYPLDSQNCTVEIES 181 >DQ667192-1|ABG75744.1| 489|Apis mellifera pH-sensitive chloride channel variant 4 protein. Length = 489 Score = 38.7 bits (86), Expect = 7e-05 Identities = 37/162 (22%), Positives = 70/162 (43%), Gaps = 8/162 (4%) Frame = +3 Query: 117 ENATSLSLMIDSLLAT--YDRE--SPPDSKIVVNLT-LHLRHANIRESESTVRILADLQM 281 E+ S ++D+LL + YD+ P + VN++ L L A+ ES + LQ Sbjct: 56 EDGKSDKEILDNLLLSTRYDKRLLPPVQGTLTVNVSVLLLSLASPDESSLKYEVEFLLQQ 115 Query: 282 NWIDKRLSW-NAGEWGYSTWLVSSERLWRPDV--VLLNAAATTAGDYALRARVSNNGSVS 452 W D RL + N ++ + + + +W PD ++ R+ NG+V+ Sbjct: 116 QWYDPRLRYSNRSQYEFLNAIHHYDDIWLPDTYFIMHGDFKDPLIPVHFALRIYRNGTVN 175 Query: 453 WIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFV 578 ++ R + +L+ +P D C+F S + + +V Sbjct: 176 YLMRRHLILSCQGRLNIFPFDDPLCSFAIESISYEQTAITYV 217 >DQ667191-1|ABG75743.1| 475|Apis mellifera pH-sensitive chloride channel variant 3 protein. Length = 475 Score = 38.7 bits (86), Expect = 7e-05 Identities = 37/162 (22%), Positives = 70/162 (43%), Gaps = 8/162 (4%) Frame = +3 Query: 117 ENATSLSLMIDSLLAT--YDRE--SPPDSKIVVNLT-LHLRHANIRESESTVRILADLQM 281 E+ S ++D+LL + YD+ P + VN++ L L A+ ES + LQ Sbjct: 56 EDGKSDKEILDNLLLSTRYDKRLLPPVQGTLTVNVSVLLLSLASPDESSLKYEVEFLLQQ 115 Query: 282 NWIDKRLSW-NAGEWGYSTWLVSSERLWRPDV--VLLNAAATTAGDYALRARVSNNGSVS 452 W D RL + N ++ + + + +W PD ++ R+ NG+V+ Sbjct: 116 QWYDPRLRYSNRSQYEFLNAIHHYDDIWLPDTYFIMHGDFKDPLIPVHFALRIYRNGTVN 175 Query: 453 WIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFV 578 ++ R + +L+ +P D C+F S + + +V Sbjct: 176 YLMRRHLILSCQGRLNIFPFDDPLCSFAIESISYEQTAITYV 217 >DQ667189-1|ABG75741.1| 458|Apis mellifera pH-sensitive chloride channel protein. Length = 458 Score = 38.7 bits (86), Expect = 7e-05 Identities = 37/162 (22%), Positives = 70/162 (43%), Gaps = 8/162 (4%) Frame = +3 Query: 117 ENATSLSLMIDSLLAT--YDRE--SPPDSKIVVNLT-LHLRHANIRESESTVRILADLQM 281 E+ S ++D+LL + YD+ P + VN++ L L A+ ES + LQ Sbjct: 56 EDGKSDKEILDNLLLSTRYDKRLLPPVQGTLTVNVSVLLLSLASPDESSLKYEVEFLLQQ 115 Query: 282 NWIDKRLSW-NAGEWGYSTWLVSSERLWRPDV--VLLNAAATTAGDYALRARVSNNGSVS 452 W D RL + N ++ + + + +W PD ++ R+ NG+V+ Sbjct: 116 QWYDPRLRYSNRSQYEFLNAIHHYDDIWLPDTYFIMHGDFKDPLIPVHFALRIYRNGTVN 175 Query: 453 WIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFV 578 ++ R + +L+ +P D C+F S + + +V Sbjct: 176 YLMRRHLILSCQGRLNIFPFDDPLCSFAIESISYEQTAITYV 217 >DQ667188-1|ABG75740.1| 383|Apis mellifera histamine-gated chloride channel protein. Length = 383 Score = 38.3 bits (85), Expect = 9e-05 Identities = 37/166 (22%), Positives = 74/166 (44%), Gaps = 12/166 (7%) Frame = +3 Query: 117 ENATSLSLMIDSLLATYDRESPPDSKIVVNLTLHLRHANIRE----SESTVRILAD--LQ 278 E++ SL ++ YD+ P K + T+ H + +E ++ +AD L Sbjct: 1 ESSLSLKDILPLNPKLYDKHRAP--KFLGQPTIVYFHVTVLSIDSINEESMTYVADIFLA 58 Query: 279 MNWIDKRLSW--NAGEWGYSTWLVS-SERLWRPDVVLLNAAATTAGDYALRAR---VSNN 440 +W D RL N E Y V +WRPD NA T + ++ + ++ Sbjct: 59 QSWRDSRLRLPENMSE-DYRILDVDWLHNIWRPDCFFKNAKKVTFHEMSIPNHYLWLYHD 117 Query: 441 GSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGSRMHNSDEMDFV 578 ++ ++ +L + +M+ +++P+D Q C+ S H + ++ F+ Sbjct: 118 KTLLYMSKLTLVLSCAMKFESYPHDTQICSMMIESLSHTTQDLVFI 163 >AF094822-1|AAC63381.1| 365|Apis mellifera GABA receptor Rdl subunit protein. Length = 365 Score = 37.5 bits (83), Expect = 2e-04 Identities = 29/96 (30%), Positives = 44/96 (45%), Gaps = 9/96 (9%) Frame = +3 Query: 285 WIDKRLSWNAGEWGYSTWLVSSE---RLWRPDVVLLNAA------ATTAGDYALRARVSN 437 W D RL++ G T V SE +W PD +N ATT+ ++ R+ + Sbjct: 6 WTDPRLAFKK-RTGVETLSVGSEFIKNIWVPDTFFVNEKQSYFHIATTSNEFI---RIHH 61 Query: 438 NGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFGS 545 +GS++ RL I+ M L +P D Q C + S Sbjct: 62 SGSITRSIRLTITASCPMNLQYFPMDRQLCHIEIES 97 >DQ667187-1|ABG75739.1| 428|Apis mellifera histamine-gated chloride channel protein. Length = 428 Score = 35.9 bits (79), Expect = 5e-04 Identities = 20/81 (24%), Positives = 37/81 (45%), Gaps = 3/81 (3%) Frame = +3 Query: 351 ERLWRPDVVLLNAAATTAGDYALRAR---VSNNGSVSWIKRLDISTPISMQLDNWPNDMQ 521 + +WRPD NA + T + + + ++ ++ +L + +M +P+D Q Sbjct: 116 KNMWRPDSFFKNAKSVTFQTMTIPNHYLWLYKDKTILYMVKLTLKLSCAMNFLIYPHDTQ 175 Query: 522 TCTFKFGSRMHNSDEMDFVID 584 C + S H +DEM F D Sbjct: 176 ECKLQMESLSHTTDEMIFQWD 196 >DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride channel variant 1 protein. Length = 509 Score = 33.9 bits (74), Expect = 0.002 Identities = 29/132 (21%), Positives = 56/132 (42%), Gaps = 4/132 (3%) Frame = +3 Query: 195 IVVNLT-LHLRHANIRESESTVRILADLQMNWIDKRLSW-NAGEWGYSTWLVSSERLWRP 368 + VN++ L L A+ ES + LQ W D RL + N ++ + + + +W P Sbjct: 137 LTVNVSVLLLSLASPDESSLKYEVEFLLQQQWYDPRLRYSNRSQYEFLNAIHHYDDIWLP 196 Query: 369 DV--VLLNAAATTAGDYALRARVSNNGSVSWIKRLDISTPISMQLDNWPNDMQTCTFKFG 542 D ++ R+ NG+V+++ R + +L+ +P D C+F Sbjct: 197 DTYFIMHGDFKDPLIPVHFALRIYRNGTVNYLMRRHLILSCQGRLNIFPFDDPLCSFAIE 256 Query: 543 SRMHNSDEMDFV 578 S + + +V Sbjct: 257 SISYEQTAITYV 268 >DQ667195-1|ABG75747.1| 469|Apis mellifera cys-loop ligand-gated ion channel subunit protein. Length = 469 Score = 31.1 bits (67), Expect = 0.014 Identities = 20/74 (27%), Positives = 35/74 (47%), Gaps = 3/74 (4%) Frame = +3 Query: 357 LWRPDVVLLN--AAATTAGDYA-LRARVSNNGSVSWIKRLDISTPISMQLDNWPNDMQTC 527 +W P V + N ++A T + + + +G V RL + ++L+ +P D+Q C Sbjct: 97 IWTPTVYVSNEPSSAITGNNVKDVLVSIDPSGMVRLNTRLQATLNCGLRLEKFPFDVQEC 156 Query: 528 TFKFGSRMHNSDEM 569 F S HN +M Sbjct: 157 PLIFESWTHNVLDM 170 >DQ667194-1|ABG75746.1| 391|Apis mellifera cys-loop ligand-gated ion channel subunit protein. Length = 391 Score = 28.3 bits (60), Expect = 0.095 Identities = 28/136 (20%), Positives = 57/136 (41%), Gaps = 11/136 (8%) Frame = +3 Query: 195 IVVNLTLHLRHAN-IRESESTVRILADLQMNWIDKRLS-----WNAGEWGYSTWLVSSER 356 ++V+ + + N I + R+ ++ +WI+ RL + G+ + + Sbjct: 11 VLVDFNIFVADINSINVEDMDFRVDMFVRQSWIESRLDMPDYIFEEGDDYVTLPPEFFDS 70 Query: 357 LWRPDVVLLNAAATTAGDYALRARVSN-----NGSVSWIKRLDISTPISMQLDNWPNDMQ 521 LW+PD LNA + AL + S+ N +V + R+ M+ +P D+Q Sbjct: 71 LWQPDPYFLNAKVSEIA--ALNHKFSSVTLYRNKTVKYSARMHAIIACQMEFQLYPMDIQ 128 Query: 522 TCTFKFGSRMHNSDEM 569 C S ++ ++ Sbjct: 129 ICPIYIESFSYHKQKL 144 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 210,275 Number of Sequences: 438 Number of extensions: 4790 Number of successful extensions: 95 Number of sequences better than 10.0: 35 Number of HSP's better than 10.0 without gapping: 50 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 55 length of database: 146,343 effective HSP length: 57 effective length of database: 121,377 effective search space used: 27552579 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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